全文获取类型
收费全文 | 81988篇 |
免费 | 5052篇 |
国内免费 | 770篇 |
专业分类
林业 | 3144篇 |
农学 | 2489篇 |
基础科学 | 676篇 |
9797篇 | |
综合类 | 16391篇 |
农作物 | 3021篇 |
水产渔业 | 3747篇 |
畜牧兽医 | 42511篇 |
园艺 | 1122篇 |
植物保护 | 4912篇 |
出版年
2021年 | 720篇 |
2020年 | 800篇 |
2019年 | 977篇 |
2018年 | 1346篇 |
2017年 | 1552篇 |
2016年 | 1378篇 |
2015年 | 1321篇 |
2014年 | 1496篇 |
2013年 | 2899篇 |
2012年 | 2860篇 |
2011年 | 3457篇 |
2010年 | 2283篇 |
2009年 | 2166篇 |
2008年 | 3076篇 |
2007年 | 2954篇 |
2006年 | 2658篇 |
2005年 | 2579篇 |
2004年 | 2306篇 |
2003年 | 2356篇 |
2002年 | 2202篇 |
2001年 | 2484篇 |
2000年 | 2528篇 |
1999年 | 1939篇 |
1998年 | 789篇 |
1997年 | 706篇 |
1995年 | 740篇 |
1992年 | 1526篇 |
1991年 | 1716篇 |
1990年 | 1640篇 |
1989年 | 1588篇 |
1988年 | 1472篇 |
1987年 | 1526篇 |
1986年 | 1573篇 |
1985年 | 1396篇 |
1984年 | 1187篇 |
1983年 | 1015篇 |
1982年 | 679篇 |
1979年 | 1056篇 |
1978年 | 829篇 |
1977年 | 672篇 |
1976年 | 711篇 |
1975年 | 746篇 |
1974年 | 1003篇 |
1973年 | 984篇 |
1972年 | 951篇 |
1971年 | 878篇 |
1970年 | 851篇 |
1969年 | 804篇 |
1967年 | 702篇 |
1966年 | 664篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
The occurrence of Mycobacterium spp. in freshwater and marine ornamental fish was studied in Italy from June 2002 to May 2005. Two surveys were carried out, one of aquarium fish sent to the Laboratory for diagnosis, and the other of prevalence of infection by mycobacteria in ornamental fish imported into Italy. Bacterial isolation was carried out from the spleen, kidney and liver, and the isolates were subsequently identified by biochemical tests. In the first survey, 387 fish were examined and Mycobacterium spp. were isolated from 181 (46.8%) fish. In the second survey 127 batches of ornamental fish from different countries were examined. Mycobacterium spp. were isolated from 38 (29.9%) batches. The following species were found: M. fortuitum, M. peregrinum, M. chelonae, M. abscessus, M. marinum, M. gordonae, M. nonchromogenicum and M. interjectum. There was a high prevalence of infection independent of the presence of macroscopic lesions. Mycobacterium fortuitum and M. chelonae were more prevalent than M. marinum in the samples examined. 相似文献
992.
C. L. SCHULTZ B. C. LIDGERDING P. E. McALLISTER F. M. HETRICK 《Journal of fish diseases》1986,9(2):117-122
Abstract. Differential incorporation of uridine and uracil was used to assay for mycoplasma contamination in five fish cell lines: bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papillosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The method was not suitable for monitoring BF-2, CHSE-214, FHM, and RTG-2 cell lines because they incorporated uracil. Differential incorporation of uridine and uracil may be applicable for screening EPC cells because only this cell line could distinguish cultures experimentally infected with Mycoplasma orale from cultures known to be free from microbial contaminants. 相似文献
993.
Provision of a suitable feed is paramount for the ongoing success of spiny lobster culture. This study compared and evaluated the performance of seven diets for first instar juvenile spiny lobster Panulirus argus [5–6 mm carapace length (CL)] based on growth rates, survival, and feed conversion ratio. Results demonstrated that a seafood-based juvenile formulation produced the fastest growth rate (3.49% weight gain day−1 and 0.90% CL increase day−1 over a 28-day period). These growth rates were also reflected by a low feed conversion rate (3.04) for this formulated feed. Similar results were also obtained for juveniles fed a frozen seafood diet, however, frozen brine shrimp, Artemia salina (both enriched and un-enriched), frozen enriched mysis shrimp, a dry pellet, and a meal-based juvenile formulation did not produce consistent growth rates. Survival rates ranged between 38% and 85% and demonstrate that juvenile lobsters have better likelihoods of survival in captive culture environments compared to the wild. Although further nutritional refinement is recommended, the results from this research have significant implication for the possible expansion of juvenile spiny lobster growout to a larger scale. 相似文献
994.
Abstract. Samples of water, sediment and fish were collected from six commercial channel catfish, Ictalurus punctalus (Rafinesque), ponds from May to October and analysed for muddy-odour producing compounds. Three episodes of off-flavour in the fish occurred in late summer. The episodes were of 2–3 months' duration and correlated with the levels of 2-methylisoborneol in water, sediment and fish. Oscillatoria agardliii was the dominant phytoplankton in ponds with off-flavour fish. Other known muddy odour-producing compounds such as geosmin, 2-isopropyl-3-mcthoxypyrazinc, 2-isobutyl-3-methoxypyrazine and 2-isobutyl-6-methoxypyrazinc were not detected in the water sediment or at concentrations greater than 5·0 ng/l. 相似文献
995.
S.H.S. Dananjaya H.P.S.U. Chandrarathna L.A.U. Nayanaransi M. Edussuriya A.S. Dissanayake Ilson Whang Mahanama De Zoysa 《Aquaculture Research》2020,51(8):3109-3118
Pectin is a biodegradable polysaccharide, and it has been recently applied as a gene delivery, drug delivery, wound healing and tissue engineering agent. In this study, pectin was extracted from pomelo (Citrus maxima) peel and characterized. The extraction recovery of pectin form pomelo peel was 14.5%, and it had 72.56% degree of esterification, 1,245.56 equivalent weight, 7.82% methoxyl and 68.27% anhydrouronic acid contents. Use of pomelo pectin as a hatching enhancing agent for fish embryos and its effect on hatching enzyme 1 (ZHE1) was investigated. The pectin‐exposed zebrafish embryos (100 µg/ml) showed significantly (p < .05) higher hatching rate (96.6%) compared with untreated (control) embryos (66.6%) at 60 hpf. The mRNA expression of ZHE1 was also significantly (p < .05) elevated up to 55.6‐fold in pectin‐exposed embryos at 24 hpf. In situ hybridization results revealed remarkably strong expression of ZHE1 in pectin‐exposed embryos compared with the control group. In addition, considerably larger size of the hatching gland was observed in pectin‐exposed larvae than that of the unexposed larvae group. These results clearly indicate that pectin isolated from pomelo peel has an ability to enhance the hatching process of zebrafish embryos via upregulation of ZHE1. 相似文献
996.
997.
Solid waste recovered from clam processing wash water was evaluated for its utilization as a replacement protein for fish meal. Three diets, diets 1 and 2 formulated to simulate the Oregon Moist Pellet (OMP) and diet 3 (Rangen), were fed to rainbow trout (Salmo gairdneri) for eight weeks. The effects of the clam waste meal on fish growth, food conversion, carcass composition and whole body fatty acid content were determined. The clam waste diet supported maximum growth and food conversion. Carcass analysis of moisture, protein and lipid contents were similar among the moist feeds. The dietary levels of 20:5w3 + 22:6w3 and total polyunsaturated fatty acids (PUFAs) were significantly higher in the clam waste diet pellet. 相似文献
998.
L.Y. Zhou B. Senthilkumaran D.S. Wang C.C. Sudhakumari T. Kobayashi H. Kajiura-Kobayashi M. Matsuda M. Yoshikuni Y. Nagahama 《Fish physiology and biochemistry》2003,28(1-4):381-382
A 548 bp partial cDNA fragment of 17β-hydroxysteroid dehydrogenase (17β-HSD1) was obtained by RT-PCR from the ovary of Nile Tilapia. The expression of 17β-HSD1 was high from 0 to 11 days after spawning, but there was a sharp decline at the day of spawning (day 14) indicating its involvement in ovarian cycle. 相似文献
999.
Abstract The tremendous loss of life and assets resulting from the 2004 tsunami dealt a devastating blow to the coastal communities of Aceh Province, Indonesia. An assessment of the fishing fleet structure pre- and post-tsunami, including associated pattern in boat aid, in 15 coastal communities was conducted and compared with data on boat relief efforts over 17 districts of the Province. Aid was found to be not proportionally allocated to losses incurred by communities and was in many cases below what could be seen as a trend toward overcapacity. The distribution of aid appeared to be done without consideration of the former structure of the fleet, which changed significantly over time (pre- and post-tsunami), and resulted in a new fleet of reduced diversity with a strong focus on the smaller boat categories. If this situation perpetuates after the initial post-disaster period, it may further exacerbate the pre-existing economic and geographic marginalisation of some remote communities, and reduce the capacities of the fleets to diversify and develop multi-species harvesting strategies; thus, increasing the risk of ecologically unsustainable exploitation in near shore areas. 相似文献
1000.
J. M. S. GONÇALVES L. BENTES R. COELHO P. MONTEIRO J. RIBEIRO C. CORREIA P. G. LINO & K. ERZINI 《Fisheries Management and Ecology》2008,15(3):199-210
Abstract Non-commercial invertebrate discards in an experimental trammel net fishery were studied in relation to selectivity of the gear, depth, soak time and season. Forty experimental fishing trials were carried out over a 1-year period with six combinations of small mesh (100, 120 and 140 mm) inner and large mesh (600 and 800 mm) outer panels. On average, 43.8 ± 12.2 (SD) (individuals 1000 m−1 of net) of non-commercial invertebrates were discarded, accounting for 48% and 65% of the total catch and total discards by numbers, respectively. Within non-commercial invertebrates discards, the six most abundant species by number were Phallusia mammillata (Cuvier) (27.5%), Cymbium olla (L.) (13.0%), Sphaerechinus granularis (Lamarck) (11.3%), Paracentrotus lividus (Lamarck) (10.9%), Astropartus mediterraneus (Risso) (8.2%) and Astropecten aranciacus (L.) (8.1%); Echinoderms (43.1%) particularly important. The highest and lowest discard ratios were found in autumn and winter, respectively. Discards generally decreased with depth, varied considerably in relation to soak time and were not related to mesh size combinations. Trammel nets seem to be the most important gear in terms of ecological impacts on benthic invertebrates compared with other coastal fishing gears and at the depths studied (15–60 m). 相似文献