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71.
Hideki TAKAHASHI Mitsuhiro SUGIYAMA SUKAMTO Akira KARASAWA Shuu HASE Yoshio EHARA 《Journal of General Plant Pathology》2000,66(4):335-344
A variant of Cucumber mosaic virus, CMV(Y/GM2), was isolated from a tobacco plant with mild green mosaic symptoms that was regenerated in vitro from a yellow strain of CMV [CMV(Y)]-infected tobacco leaves by tissue culture. CMV(Y/GM2) has two amino acid substitutions
at 36 and 111 positions in the coat protein encoded on RNA3. CMV, assembled by mixing in vitro transcribed CMV(Y) RNA1 and RNA2 plus infectious RNA3 transcribed in vitro from cDNA to RNA3 of CMV(Y/GM2), was prepared and designated as CMV(Y/GM2)tr. When tobacco (Nicotiana tabacum cv. Xanthi nc) plants were inoculated with CMV(Y/GM2)tr, large necrotic local lesions in which the virus was localized, developed
on the inoculated leaves. This host response unique to CMV(Y/GM2)tr was similar to the hypersensitive response (HR), which
is a common resistance response to avirulent pathogens and was observed in five cultivars of Nicotiana tabacum and eight Nicotiana species. The revertant virus, however, accumulated to quite different levels in the various hosts. CMV(Y/GM2)tr induced pathogenesis-related
1 (PR-1) protein accumulation and systemic acquired resistance (SAR) which were generally observed in the HR. However, when
tobaccos were inoculated with CMV(S36P)tr and CMV(V111I)tr, which have an amino acid substitution at either the 36 or 111
position in the coat protein of CMV(Y), respectively, CMV(S36P)tr was restricted to the primary infection site without necrotic
local lesion formation and PR-1 protein and SAR induction. CMV(V111I)tr, however, systemically spread and induced mild green
mosaic symptoms, while the host had the HR to CMV(Y/GM2)tr. The localization of CMV(Y/GM2)tr at the primary infection site
may not only be caused by the HR, but also by the restriction of virus systemic movement resulting from the amino acid substitution
at position 36 in the coat protein of CMV(Y).
Received 15 December 1999/ Accepted in revised form 18 April 2000 相似文献
72.
Yu FURUSAWA Masashi TAKAHASHI Mariko SHIMA-SAWA Hitoshi HATAI Noriaki MIYOSHI Osamu YAMATO Akira YABUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1363
Epithelial–mesenchymal transition (EMT) plays a crucial role in metastasis of epithelial tumors; however, it is challenging to detect EMT by cytology. In the present study, EMT was visualized by fluorescence-immunocytochemistry (FICC). Air-dried smears from epithelial tumors of dogs (n=22) and cats (n=9) were stained using mouse monoclonal anti-E-cadherin and rabbit monoclonal anti‐vimentin antibodies. Enzymatic immunohistochemistry (IHC) revealed that 51.6% (8/22 in dogs, 8/9 in cats) of the cases showed EMT. In dogs, FICC could detect EMT in 62.5% (5/8) of those cases. In cats, FICC could detect EMT in 100% (8/8) of the cases. In conclusion, the present FICC method could successfully detect EMT using conventional air-dried cytology smear slides. 相似文献
73.
Satoshi AKAGI Kazutsugu MATSUKAWA Seiya TAKAHASHI 《The Journal of reproduction and development》2014,60(5):329-335
Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle synchronization of donor
cells, enucleation, cell fusion, oocyte activation and embryo culture. Therefore, many factors are believed to contribute to
the success of embryo development following nuclear transfer. Numerous attempts to improve cloning efficiency have been
conducted since the birth of the first sheep by somatic cell nuclear transfer. However, the efficiency of somatic cell
cloning has remained low, and applications have been limited. In this review, we discuss some of the factors that affect the
developmental ability of somatic cell nuclear transfer embryos in cattle. 相似文献
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进行了可用于治沟骨干坝的3种不同坡度的台阶式泄水建筑物的模型试验。结果表明,在跌落水流时,消力池跃前断面单位能量随斜坡变陡和相对坝高Hdam/yc增加而增大,但当Hdam/yc增加到某一值后,逐渐趋于平缓。消力池跃前断面底部压力yp与堰上临界水深yc呈现正比线性相关,yp大小与台阶个数N无关。比较跃前断面压力修正系数λ>1和λ=1时的消能率,当Hdam/yc=10左右时,其消能率相对误差可达7%左右,但当Hdam/yc≥70时,其相对误差则降至1%。试验结果也同时显示出λ与yp/y1同样有着很好的线性关系,其相关系数r=0.99999。 相似文献
78.
Hiroyuki NAKAYAMA Tetsuya TAKAHASHI Kaori NAKAGAWA‐MIZUYACHI Mitsuo KAWASHIMA 《Animal Science Journal》2011,82(3):475-480
The presence of a receptor for calcitonin (CT) and the effect of chicken CT (cCT) on adrenocorticotropic hormone (ACTH) secretion stimulated by rat/human corticotropin‐releasing hormone (rhCRH) in the hen anterior pituitary were studied. The specific [125I]cCT binding component was present in the plasma membrane of hen anterior pituitary and this binding component had properties of a receptor which has binding specificity to cCT, reversibility, saturable binding, high affinity and limited capacity. When anterior pituitary cells were incubated in vitro, cCT increased the maximal secretion of chicken ACTH stimulated by rhCRH. These results suggest that CT may act directly on the anterior pituitary via its receptor binding and enhances the ACTH secretion by CRH. 相似文献
79.
Sanae FUKUSHIMA Kimie NIIMI Eiki TAKAHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):161-166
The classic piebald mutation in the endothelin receptor type B (Ednrb) gene was found on rolling Nagoya genetic background (PROD-s/s) mice with white coat spotting. To examine whether genetic background influenced the phenotype in the piebald mutant mice, we generated a congenic strain (B6.PROD-s/s), produced by repeated backcrosses to the C57BL/6J (B6) strain. Although B6.PROD-s/s mice showed white coat spotting, 7% of B6.PROD-s/s mice died between 2 and 5 weeks after birth due to megacolon. The PROD-s/s, s/s and Japanese fancy mouse 1 (JF1) strains, which also have piebald mutations on different genetic backgrounds with B6, showed only pigmentation defects without megacolon. In expression analyses, rectums of B6.PROD-s/s
with megacolon mice showed ~5% of the level of Ednrb gene expression versus B6 mice. In histological analyses, aganglionosis was detected in the rectum of megacolon animals. The aganglionic rectum was thought to lead to severe constipation and intestinal blockage, resulting in megacolon. We also observed an abnormal intestinal flora, including a marked increase in Bacteroidaceae and Erysipelotrichaceae and a marked decrease in Lactobacillus and Clostridiales, likely inducing endotoxin production and a failure of the mucosal barrier system, leading ultimately to death. These results indicate that the genetic background plays a key role in the development of enteric ganglion neurons, controlled by the Ednrb gene, and that B6 has modifier gene (s) regarding aganglionosis. 相似文献
80.