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101.
Murakami A Gao G Kim OK Omura M Yano M Ito C Furukawa H Jiwajinda S Koshimizu K Ohigashi H 《Journal of agricultural and food chemistry》1999,47(1):333-339
Three known coumarins have been isolated from Citrus hystrix DC as inhibitors of both lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma)-induced nitric oxide (NO) generation in RAW 264.7 cells. The inhibitory activity of bergamottin (IC(50) = 14 microM) was comparable to that of N-(iminoethyl)-L-ornithine (L-NIO) (IC(50) = 7. 9 microM), whereas oxypeucedanin and 5-[(6',7'-dihydroxy-3', 7'-dimethyl-2-octenyl)oxy]psoralen, structurally different from bergamottin only in their side-chain moieties, were notably less active. Using 21 coumarins, we structurally classified various types of coumarins into groups A-C: (A) bearing an isoprenyl (IP) or a geranyl (GR) group, highly active; (B) bearing an IP group cyclized to a coumarin ring, moderately active; (C) bearing an IP group modified with hydroxyl group(s) and/or having other functional groups except for the IP, completely inactive. Cellular uptake studies suggested that coumarins in group C are inactive because of poor permeability to the cell membrane. 相似文献
102.
Satoshi Furukawa Yumiko Hoshikawa Kota Irie Yusuke Kuroda Kazuya Takeuchi 《Journal of toxicologic pathology》2021,34(2):157
A swim bladder tumor was detected in one scoliotic medaka aged 22 weeks. The tumor was located in the dorsal abdominal cavity, with maximum dimension of 1,850 × 1,500 µm. No swim bladder lumen was identified, and the region where the swim bladder lumen would have been located, was replaced with adipose tissues. The tumor was a non-invasive, expansile, and encapsulated solid mass with a few cysts, and comprised a homogenous population of well-differentiated, densely packed, gas glandular epithelium-like cells. The tumor mass was connected to a rete mirabile that showed a hyperplastic capillary plexus; however, the tumor cells did not invade the rete mirabile, thereby revealing that the tumor was an adenoma originating from the gas glandular epithelium of the swim bladder. Since proliferative lesions in the swim bladder have been reported in some teleosts with skeletal deformations, including medaka, the occurrence of a spontaneous swim bladder tumor in teleosts is considered to be closely associated with various types of skeletal deformation, and spinal curvature in particular. 相似文献
103.
104.
Mitsuki Uemura Kazuhiko Ochiai Masami Morimatsu Masaki Michishita Eri Onozawa Daigo Azakami Yumiko Uno Yasunaga Yoshikawa Takanori Sasaki Masami Watanabe Toshinori Omi 《Veterinary and comparative oncology》2020,18(2):247-255
RAD51 forms a complex with BRCA2 and plays a central role in the DNA damage response pathway that is associated with homologous recombination. The structures of RAD51 and its homologues are highly conserved from prokaryotes to higher eukaryotes. Although a large number of BRCA2 mutations have been reported, there are only a few reports on the mutations of RAD51, which have been shown in humans and dogs. However, several mutations of canine RAD51 were identified from mammary gland tumour tissues in a recent study. Some of these mutations seem to have an influence on the homo‐oligomerization or interaction with “Partner and localizer of BRCA2” (PALB2). In this study, we cloned the canine PALB2 homologue and investigated the effect on its interaction with the RAD51 mutants to evaluate the alteration in the function of RAD51 mutants. The A209S and T225S mutants of RAD51 show an attenuation of the interaction between RAD51 and PALB2. These results indicate that the canine RAD51 mutations can potentially alter the homologous recombination pathways in response to DNA damage in dogs. 相似文献
105.
Furukawa S Hayashi S Usuda K Abe M Hagio S Ogawa I 《Journal of toxicologic pathology》2011,24(2):95-111
The placenta grows rapidly for a short period with high blood flow during pregnancy and has multifaceted functions, such as its barrier function, nutritional transport, drug metabolizing activity and endocrine action. Consequently, the placenta is a highly susceptible target organ for drug- or chemical-induced adverse effects, and many placenta-toxic agents have been reported. However, histopathological examination of the placenta is not generally performed, and the placental toxicity index is only the placental weight change in rat reproductive toxicity studies. The placental cells originate from the trophectoderm of the embryo and the endometrium of the dam, proliferate and differentiate into a variety of tissues with interaction each other according to the development sequence, resulting in formation of a placenta. Therefore, drug- or chemical-induced placental lesions show various histopathological features depending on the toxicants and the exposure period, and the pathogenesis of placental toxicity is complicated. Placental weight assessment appears not to be enough to evaluate placental toxicity, and reproductive toxicity studies should pay more attention to histopathological evaluation of placental tissue. The detailed histopathological approaches to investigation of the pathogenesis of placental toxicity are considered to provide an important tool for understanding the mechanism of teratogenicity and developmental toxicity with embryo lethality, and could benefit reproductive toxicity studies. 相似文献
106.
Takuro Ito Itaru Konno Shosei Kubota Toshinori Ochiai Takahiro Sonoda Yuko Hayashi Tatsuya Fukuda Jun Yokoyama Hokuto Nakayama Toshiaki Kameya Akira Kanno 《Euphytica》2011,182(2):285-294
Interspecific hybridization is a useful technique to introduce characteristics from wild species into crops. Garden asparagus (Asparagus officinalis, 2n = 2x = 20) is an economically important vegetable that is native to the Mediterranean region but widely cultivated in many countries. The genus Asparagus is comprised of over 100 species. Asparagus kiusianus (2n = 2x = 20) is a wild asparagus species endemic to Japan. This species occurs on the coast and is likely to be a salt-resistant species. Although the geographic distribution of these two species is not close, molecular phylogenetic analysis demonstrated that these two species are closely related. In this study, a reciprocal cross between A. officinalis and A. kiusianus was carried out by hand pollination, and progeny were obtained from both crossings. These progeny exhibited a morphologically intermediate phenotype in terms of flower shape, and restriction fragment length polymorphism analysis confirmed that these were indeed interspecific hybrids. The interspecific hybrids were fertile, and backcross progeny with garden asparagus was also generated. These interspecific hybrids are expected to supply novel traits to garden asparagus. 相似文献
107.
108.
Toshiyuki Wakatsuki Hisao Furukawa Keizaburo Kawaguchi 《Soil Science and Plant Nutrition》2013,59(4):351-360
The authors reported that the relative bonding strength between ligand of soil colloid surface and cations could be obtained easily by the measurement of MCSA, and that the MCSA corresponded to the constant of Langmuir's adsorption isotherm equation. The relative bonding strength of cations with respect to kaolinitic soil clay at pH 6 was, Cr3+>Fe3+, Al3+>Ga8+>Cu8+>Pb2+>Y3+, La3+>Mn2+>Ni2+, Co2+> Zn2+>Sr2+, Mg2+>NH4+, K+, and with respect to colloid with humus coating, Y3+, La3+>Pb2+>Cu2+, and the other orders were same. The solubility of cations in soil colloid aqueous dispersion system was calculated from the values of MCSAs, and considered as follows, Y3+, La3+, Cu3+, Pb3+, Mn2+, Ni2+, CO2+: concentration in soil solution and soil geochemical mobility may be regulated by the specific adsorption reaction, Zn2+, Mg2+, Sr2+, K+, NH4+: concentration in soil solution and soil geochemical mobility may be regulated by the non-specific adsorption reaction, but at neutral to alkaline condition, Zn2+ and Mg2+ may specifically adsorb on soil, clays, Fe3+, Cr3+, Al3+, Ga3+: concentration in soil solution and soil geochemical mobility may be regulated by the solubility of their oxide hydrates. 相似文献
109.
Charles I. Sagoe Tadao Ando Kenji Kouno Toshinori Nagaoka 《Soil Science and Plant Nutrition》2013,59(4):617-625
A series of experiments was conducted to quantify the relative contribution of protons and other mechanisms to the dissolution of phosphate rocks (PRs) from six countries in solutions of low-molecular-weight-aliphatic organic acids. The amounts of P and Ca released after 3 d of incubation at 28°C were determined in all the experiments. In the first experiment the solubility of the PRs < 500 μm particle size) in 100 M (25 mL g-1 PR) oxalic, tartaric, and citric acids was compared with that in three mineral acids and four chelating compounds. There were no differences in the amount of P released by the mineral acids, but the organic acids released more P than could be accounted for by protonation. The chelating compounds were the least effective. In the second experiment, 1 g each of Sri Lanka and Togo PRs was incubated with 2.5 mmol of the organic acids using three acid concentration (mM) : acid volume ratios (250:10, 100:25, and 25:100). The amount of P dissolved from the PRs and the amount of acid remaining in the filtrate were about the same for all three treatments. A mixture of the organic acids and the respective Na-salts was prepared at six different acid: salt ratios in the third experiment. Total acid concentration in all the solutions was 100 mM. Higher proportion of the Na-salt in the mixture decreased PR dissolution, while the amount of the acid remaining in the filtrate increased. The Ca concentration in the filtrate showed an opposite trend. The results indicated that PR dissolution by the organic acids was initially dependent on protonation, which accounted for only 13–38% of the amount of P dissolved. Most of the amount of P dissolved could therefore be attributed to other mechanisms. Removal of dissolved Ca from the solution appears to be the major factor controlling the subsequent dissolution of the PRs. The ability of tartaric and oxalic acids to dissolve effectively the PRs was attributed to the formation of an insoluble calcium compound which was precipitated from the solution. For the use of organic acids as a potential amendment for improving the P availability of PRs, the Ca binding power of the organic acids should also be considered. 相似文献
110.