全文获取类型
收费全文 | 297篇 |
免费 | 4篇 |
国内免费 | 2篇 |
专业分类
林业 | 22篇 |
农学 | 16篇 |
38篇 | |
综合类 | 12篇 |
农作物 | 14篇 |
水产渔业 | 50篇 |
畜牧兽医 | 137篇 |
园艺 | 1篇 |
植物保护 | 13篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 8篇 |
2020年 | 4篇 |
2019年 | 2篇 |
2018年 | 7篇 |
2017年 | 4篇 |
2016年 | 12篇 |
2015年 | 13篇 |
2014年 | 27篇 |
2013年 | 33篇 |
2012年 | 14篇 |
2011年 | 21篇 |
2010年 | 8篇 |
2009年 | 11篇 |
2008年 | 11篇 |
2007年 | 20篇 |
2006年 | 18篇 |
2005年 | 14篇 |
2004年 | 21篇 |
2003年 | 27篇 |
2002年 | 13篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1998年 | 3篇 |
1996年 | 1篇 |
1988年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有303条查询结果,搜索用时 15 毫秒
71.
Yasuyuki TANAHASHI Ban WANG Yuri MURAKAMI Toshihiro UNNO Hayato MATSUYAMA Hiroshi NAGANO Seiichi KOMORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):203-211
In order to investigate the effects of (SKF), which is a non-selective cationic channel blocker, on
K+ channel currents, we recorded currents through ATP sensitive K+ (IKATP),
voltage-gated K+ (IKv) and Ca2+ activated K+ channels
(IBK) in the absence and presence of SKF in single small intestinal myocytes of mice with
patch-clamp techniques. SKF (10 µM) reversibly abolished IKATP that was induced by
cromakalim (10 µM), which is a selective ATP sensitive K+ channel opener. These
inhibitory effects were induced in a concentration-dependent and voltage-independent manner. The 50%
inhibitory concentration (IC50) was 0.85 µM, which was obviously lower than that
reported for the muscarinic cationic current. In addition, SKF (1 µM ≈ the IC50
value in IKATP suppression) reversibly inhibited the IKv that was induced by repetitive
depolarizing pulses from −80 to 20 mV. However, the extent of the inhibitory effects was only ~30%. In
contrast, SKF (1 µM) had no significant effects on spontaneous transient IBK and
caffeine-induced IBK. These results indicated that SKF inhibited ATP sensitive K+
channels and voltage-gated K+ channels, with the ATP sensitive K+ channels being more
sensitive than the voltage-gated K+ channels. These inhibitory effects on K+ channels
should be considered when SKF is used as a cationic channel blocker. SKF96365相似文献
72.
Correlated responses of respiratory disease and immune capacity traits of Landrace pigs selected for Mycoplasmal pneumonia of swine (MPS) lesion 下载免费PDF全文
Toshihiro Okamura Kouki Maeda Wataru Onodera Hiroshi Kadowaki Chihiro Kojima‐Shibata Eisaku Suzuki Hirohide Uenishi Masahiro Satoh Keiichi Suzuki 《Animal Science Journal》2016,87(9):1099-1105
Five generations of Landrace pigs selected for average daily gain, backfat thickness, Mycoplasmal pneumonia of swine (MPS) lesion score, and plasma cortisol levels, was executed to decrease the MPS lesion score. Genetic parameters and correlated genetic responses for respiratory disease and peripheral blood immune traits were estimated in 1395 Landrace pigs. We estimated the negative genetic correlation of MPS lesion score with phagocytic activity (PA) at 7 weeks of age (‐0.67). The breeding values of PA at 7 weeks of age and 105 kg body weight and the correlated selection response of the ratio of granular leukocytes to lymphocytes at 105 kg body weight were significantly increased, and sheep red blood cell‐specific antibody production (AP) was significantly decreased in a selection‐dependent manner. Increasing of natural immunological indicators (e.g. PA) and decreasing of humoral immunological indicator (e.g. AP) were observed due to genetically decreasing MPS lesion score. 相似文献
73.
Tomoki Honryo Tokihiko Okada Michio Kurata Toshihiro Tamura Yasunori Ishibashi 《Aquaculture Research》2014,45(7):1109-1115
We have previously reported that night‐time lighting prevents the mass death of Pacific bluefin tuna (PBT) juvenile due to collision and/or contact with the walls of sea cages, immediately after transfer to the cages, and that night‐time lighting does not negatively impact fish stress levels. However, the period of night‐time lighting should be limited to minimize negative impacts on the surrounding environment and aid management. Therefore, we investigated the optimal period of night‐time lighting by evaluating the whole‐body cortisol and glucose levels as stress parameters, growth performance and survival of PBT juvenile in four cages with different periods of night‐time lighting (i.e. unlit, 4‐day, 8‐day and 12‐day lighting). The results showed that almost all fish were died 1 day after transfer to the unlit cage. In comparison, the other groups (4‐day, 8‐day and 12‐day lighting) had high survival rates (92.5–96.0%) without significant difference. However, in the 4‐day‐lighting group, an obvious stress response was recorded on day 5, and growth performance was significantly lower. In the 8‐day‐lighting group, whole‐body cortisol levels were slightly elevated on day 9; however, significantly elevation was not recorded on day 12. These results indicate that the recommended lighting period of night‐time lighting in sea cages is 8–12 days. 相似文献
74.
Tomonori Kume Yuka Onozawa Hikaru Komatsu Kenji Tsuruta Yoshinori Shinohara Toshihiro Umebayashi Kyoichi Otsuki 《Forest Ecology and Management》2010
The applicability of sap flux (Fd) measurements to bamboo forests has not been studied. This study was undertaken to establish an optimal and effective design for stand-scale transpiration (E) estimates in a Moso bamboo forest. To this aim, we validated Fd measurements in Moso bamboos in a cut bamboo experiment. In addition, we analyzed how sample sizes affect the reliability of E estimates calculated from Fd and conducting culm area (AS_b). In the cut bamboo experiments, we found that Fd measurement using a 10 mm probe was a valid means of determining the water-use behavior of a Moso bamboo, although a specific correction was needed. Furthermore, we calculated E from stand AS_b (AS_stand) and mean stand Fd (JS). Employing Monte Carlo analysis, we examined potential errors associated with sample size in E, AS_stand, and JS using an original dataset with AS_b and Fd measured for 40 and 16 individuals, respectively. Consequently, we determined the optimal sample size for both AS_stand and JS estimates as 11. The optimal sample sizes for JS were almost the same under different vapor pressure deficit and soil moisture conditions. The optimal sample size for JS at the study site was less than that of a coniferous plantation in the same region probably owing to small individual-to-individual variations in sap flux in the Moso bamboo forest. Our study concludes that sap flux measurements are an applicable technique for assessing water use in Moso bamboo forests. 相似文献
75.
Thermal denaturation and autolysis profiles of myofibrillar proteins of mantle muscle of jumbo squid Docidicus gigas 总被引:1,自引:0,他引:1
KUNIHIKO KONNO CHO YOUNG-JE TAKEYA YOSHIOKA PARK SHINHO NOBUO SEKI 《Fisheries Science》2003,69(1):204-209
ABSTRACT: Jumbo squid was very similar to Japanese common squid in terms of myofibrillar Ca2+ -, Mg2+ - and K+ (EDTA)-ATPase activities. Myofibrils of jumbo squid were significantly stabilized upon addition of Ca2+ and destabilized by increasing KCl concentration for heating. Incubation of muscle homogenate of jumbo squid induced a selective cleavage of myosin into two major fragments and the cleavage was inhibited by EDTA. Autolysis was prominent at and above 0.3 M NaCl where myosin filaments dissolve. The enzyme involved in the autolysis was proved to be unstable showing maximal autolysis rate at 25°C. Washing the homogenate partially reduced the autolysis activity. 相似文献
76.
Toshihiro Yamada 《Journal of Forest Research》2001,6(3):127-137
When pathogenic microorganisms invade living sapwood of woody plants, a series of defense responses occurs at the lesion margin.
Putative active defense mechanisms include constitutive and induced inhibitory compounds, cell wall alterations, and occlusion
of xylem elements. Active defenses play an important role in the sapwood, while constitutive and induced microenvironmental
conditions in the wood might also constrain pathogen development. It is necessary to develop a unified understanding, in which
these factors could act synergistically and provide effective defense barriers. 相似文献
77.
Takashi?OkuyamaEmail author Javier?Doldán Hiroyuki?Yamamoto Toshihiro?Ona 《Journal of Wood Science》2004,50(1):1-6
The occurrence of heart splitting during the crosscutting of logs was discussed in relation to the released strain on Eucalyptus spp. logs. The strains released in the longitudinal and tangential directions were measured by the strain-gauge method and were correlated with the length of the heart split measured on the same logs. There were differences in the longitudinal strain; however, no significant correlation was found with the diameter that could be converted to a mean annual increment (i.e., a relation with the growth rate). The initial splits expand with the time after felling. The longer the initial split, the longer is the length 1 week after felling. The split length was significantly smaller at the butt end of the first log of every tree than at the other end, but there were no significant differences between the split length at the top of the first logs and at either end of the second logs, although there were differences among individual trees. The length of the heart split correlated with the released strain near the pith, which was estimated using Kublers equation. The longitudinal released strain measured on the surface of logs is a good indicator of the heart splitting when crosscutting logs.Part of this paper was presented at the 52nd Annual Meeting of the Japan Wood Research Society 相似文献
78.
Fujino T Matsuo T Okada M Matsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1191-1193
Detection rates from the samples including a small number of Cryptosporidium parvum oocysts were compared between the sugar flotation and the sugar centrifugal flotation methods. As the results, the oocysts were detected from 70 and 80 of 100 samples including 6.0x10(2) and 1.0x10(3) oocysts per 1 ml by the flotation method, respectively, whereas from 52 and 53 of the same samples by the centrifugal flotation method. Therefore, it was considered that the flotation method is the most suitable method for the detection from samples including a small number of Cryptosporidium oocysts. It is also suggested that results of the sugar flotation method were reliable for samples including more than 1.0x10(3) oocysts/ml. 相似文献
79.
Mitsuhisa Baba Shintaro Abe Makiko Kasai Toshihiro Sugiura Hiroshi Kobayashi 《Journal of Forest Research》2011,16(6):446-455
Understory vegetation may affect nitrate (NO3
−) leaching, even in coniferous forests. Our objective was to estimate the contribution of understory vegetation to nutrient
cycling, especially nitrogen, in a Japanese cedar (Cryptomeria japonica) stand. We therefore cut down and removed understory vegetation in one plot of the stand (the cutting plot) to compare nutrient
budgets in the cutting plot with those in a control plot in which understory vegetation was allowed to grow. We also examined
neutralization of the acid produced due to an increase in NO3
− leaching. A monitoring study on precipitation and soil-percolated water was carried out in both plots. When the understory
vegetation was cut down, NO3
− flux at a soil depth of 10 cm increased remarkably in summer, with values significantly higher than those in the control
plot. This resulted in an increase in proton load associated with N transformation ([H+]load). The increase in [H+]load enhanced mobilization of Ca2+, Mg2+, and SiO2 ([SiO2]mob). In addition, the correlations between [SiO2]mob and mobilization of each base cation were distinct in the cutting plot. These results indicated that the acids produced
because of N transformation were buffered not only by ion exchange but also by chemical weathering. The contribution of understory
vegetation to minimizing NO3
− leaching suggested that understory vegetation might reduce the risk of N saturation because of chronic atmospheric N inputs. 相似文献