山羊痘病毒SS株5个ANK基因缺失的重组病毒构建

为研究锚定蛋白基因(Ankyrin,ANK)对山羊痘病毒的影响,试验采用融合PCR和Overlap PCR技术扩增山羊痘病毒SS株5个ANK基因(ANK010、ANK138、ANK 140、ANK141.2和ANK145)两端侧翼序列和绿色荧光蛋白(GFP)基因,并将其产物连接Trans1-T 1载体构建ANK缺失的转...     

小菊'类锌指蛋白'基因植物表达载体构建及对烟草的转化

为了建立耐盐碱小菊(Chrysanthemum morifolium)中克隆到的'类锌指蛋白基因'CmSTZF(DQ864730)的烟草遗传转化体系,并研究其抗逆特性。本研究借助Gateway技术构建了小菊'类锌指蛋白'基因CmSTZF的过量表达载体pH7WG2D-CmSTZF,并以烟草NC89(N.plumbaginifolia'NC89')的叶片为试材,通过农杆菌介导的转化,成功地建立了烟草遗传转化体系,经PCR-Southern鉴定获得CmSTZF的转基因烟草株系。     

稳定作业下道床动态行为的离散元模拟与试验研究

针对稳定作用下有砟道床的研究.采用具有真实几何形状的簇颗粒模拟道碎,建立简化缩小比1:8稳定作业下道床三维离散元模型,模拟分析不同激振频率作用下道床的宏细观动态行为.建立与轨枕模型同尺寸的试验模型,通过试验与仿真分析中轨枕的沉降量对比验证,确定试验以及构建的轨枕-道床模型的有效性与可靠性.进一步利用该试验分析道砟振动加...     

玉米叶绿体表达载体构建及绿色荧光蛋白基因瞬时表达检测

Tps1是生物保护物质海藻糖的关键合酶基因.构建含Tps1的玉米叶绿体表达载体,首定从玉米叶绿体基因组中克隆16S/trnI-trnA/23S片段,作为定点整合同源片段;然后将编码酵母海藻糖合酶基因Tps1表达盒(Prrn-Tps1-TpsbA-ter)、草丁膦抗性基因Bar表达盒(RpsbApro-Bar-RpsbAter)、卡那霉素抗性基因Npt Ⅱ和绿色荧光蛋白基因Gfp构建的融和基因表达盒(Prrn-Npt Ⅱ/Gfp-RrbcLter)一起克隆到定点整合同源片段之间,构建了玉米叶绿体的稳定表达载体mTKGB.通过基因枪轰击法将mTKGB转化到玉米幼嫩叶片中,培养2 d后,在激光扫描共聚焦显微镜下脱测到玉米一些细胞的叶绿体中有很强的GFP绿色荧光,结果表明构建的载体可在玉米叶绿体中表达.     

Alteration of apoptotic susceptibility and bcl-2 gene in malignant transformation of human bronchial epithelial cells

AIM: To demonstrate the susceptibility of cell apoptosis varies during the progress of cell malignant transformation from human being in vitro. METHODS: A SV₄₀T-transfected human bronchial epithelial immortalized cell line (called M) was selected in this work, which has acquired some characteristics of malignant transformation at the later passage. The alterations of apoptosis and bcl-2, P53 genes between early and later passage of M cells were investigated by means of TDT labeling in situ, chromosome FISH, RNA and protein testing, etc. RESULTS: Incidence of apoptosis induced by cis-platin was significantly lower in later than in early passages of M. Levels of bcl-2 mRNA and protein in later passages were higher than early passages of M, and overexpression of bcl-2 was accumulated following the development of cellular malignancy. P53 protein level was as high in early as in later passages. CONCLUSION: Overexpression of bcl-2 decreases the cellular sensitivity to apoptotic inductors plays an important role during progress of carcinogenesis in human bronchial epithelial cancers. The inactivation of P53 protein in the SV₄₀-T transfected M cell line may be one of reasons of bcl-2 overexpression, but not associated with the accumulation of bcl-2 expressed level during cell transformation.     

中央储备粮调控传导影响因素分析

中央储备粮调控目标是应对粮食危机——实现市场粮食价格稳定和紧急供给。实现这一目标的基本手段是粮食实物吞吐,而保证这一基本手段的有效落实,既需要研究影响粮食实物吞吐的各种因素,还需要了解如何保证调控传导过程的畅通。影响调控传导过程的因素包括内部因素和外部因素,它们共同作用于储备粮调控传导过程。理解和优化调控传导影响因素,有利于平衡市场供需,改变市场主体的当期(主要在流通环节,如粮食经营者)和预期(主要在生产环节,如粮食生产者)行为,防止因粮食市场过度失衡,造成潜在的生产、流通、分配的重大损失。     

Integration of meta-QTL discovery with omics:Towards a molecular breeding platform for improving wheat resistance to Fusarium head blight

Fusarium head blight (FHB) is a global wheat disease that devastates wheat production. Resistance to FHB spread within a wheat spike (type II resistance) and to...     

Youssoufenes A2 and A3, Antibiotic Dimeric Cinnamoyl Lipids from the ΔdtlA Mutant of a Marine-Derived Streptomyces Strain

Two new dimeric cinnamoyl lipids (CL) featuring with an unusual dearomatic carbon-bridge, named youssoufenes A2 (1) and A3 (2), were isolated from the ΔdtlA mutant strain of marine-derived Streptomyces youssoufiensis OUC6819. Structures of the isolated compounds were elucidated based on extensive MS and NMR spectroscopic analyses, and their absolute configurations were determined by combination of the long-range NOE-based ¹H-¹H distance measurements and ECD calculations. Compounds 1 and 2 exhibited moderate growth inhibition against multi-drug-resistant Enterococcus faecalis CCARM 5172 with an MIC value of 22.2 μM.     

西北旱区石羊河流域作物耗水点面尺度转化方法的研究

基于DEM与GIS空间分析功能研究了石羊河流域主要农作物春小麦需水量ET_c的时空分异规律。根据8个气象站近50年气象资料,应用1998年FAO推荐的Penman-Monteith公式计算参考作物蒸发蒸腾量ET₀,由收集到的春小麦需水量试验资料获得多年平均作物系数K_c。近50年来流域上游的古浪、天祝春小麦全生育期ET_c呈微弱的增加趋势,中游的凉州区表现出极显著的减少趋势,其他站减少趋势不显著。确立了ET_c与海拔高度、纬度、坡向的多元回归关系,借助Arcview3.3、ArcGIS9.0与Visual Basic6.0软件实现了春小麦ET_c的空间尺度转换,并分析了石羊河流域25%、50%、75%三个不同水文年春小麦ET_c的空间变异情况。石羊河流域春小麦ET_c由山区向绿洲平原递增,多年平均值为270～591 mm。估计值与计算值相差在11.1%以内。     

湿地公园及昆明地区湿地园林的调查

主要阐述了昆明湿地园林的园林布局、表现类型及所具有的特色,分析了昆明湿地园林存在的问题和今后的发展趋势,对昆明湿地园林的改造和昆明城市建设提出了新颖的观点和展望。