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排序方式: 共有69条查询结果,搜索用时 15 毫秒
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33.
Detection of radiation-induced apoptosis using the comet assay 总被引:2,自引:0,他引:2
Wada S Khoa TV Kobayashi Y Funayama T Yamamoto K Natsuhori M Ito N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(11):1161-1166
The electrophoresis pattern of apoptotic cells detected by the comet assay has a characteristic small head and spread tail. This image has been referred to as an apoptotic comet, but it has not been previously proven to be apoptotic cells by any direct method. In order to identify this image obtained by the comet assay as corresponding to an apoptotic cell, the frequency of appearance of apoptosis was examined using CHO-K1 and L5178Y cells which were exposed to gamma irradiation. As a method for detecting apoptosis, the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay was used. When the frequency of appearance of apoptotic cells following gamma irradiation was observed over a period of time, there was a significant increase in appearance of apoptosis when using the TUNEL assay. However, there was only a slight increase when using the comet assay. In order to verify the low frequency of appearance of apoptosis when using the comet assay, we attempted to use the TUNEL assay to stain the apoptotic comets detected in the comet assay. The apoptotic comets were TUNEL positive and the normal comets were TUNEL negative. This indicates that the apoptotic comets were formed from DNA fragments with 3'-hydroxy ends that are generated as cells undergo apoptosis. Therefore, it was understood that the characteristic pattern of apoptotic comets detected by the comet assay corresponds to cells undergoing apoptosis. 相似文献
34.
Kenji TSUKANO Kazuyuki SUZUKI Toshio SHIMAMORI Ayano SATO Katsunori KUDO Ryuji ASANO Tadaharu AJITO Jeffrey LAKRITZ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):67-73
The aim of the present study was to investigate the relationships between serum
amino acid profiles in normal and calves with Mycoplasma
bronchopneumonia. Serum free amino acid concentrations in serum obtained from 34 calves
with or without Mycoplasma bronchopneumonia were determined by
high-performance liquid chromatography. The calves with Mycoplasma were
characterized by significantly lower total amino acid and total essential amino acid
concentrations and molar ratios of branched-chain amino acid (BCAA) to aromatic amino acid
(BCAA/AAA) and BCAA to tyrosine (BTR), and by a significantly higher molar ratio of serine
phosphorylation (SPR). The proposed diagnostic cutoffs for BCAA/AAA, BTR and SPR in serum
based on ROC analysis for detection of catabolic states associated with
Mycoplasma bronchopneumonia were set at <1.75, <2.86 and
>0.85, respectively. Our results suggest that determining the profiles of amino acids,
especially BTR and SPR, could provide useful diagnostic information in terms of predicting
protein catabolism in Mycoplasma bronchopneumonia. 相似文献
35.
水稻品种孕穗期耐冷性构成因子分析 总被引:27,自引:2,他引:25
在冷温处理条件下,通过对14个不同耐冷性水稻品种的花药大小、花粉大小和花粉数的测量分析,明确了花药长度与花药内的总花粉数密切相关(r=0.831**),花药长度和花药内的可育花粉数与耐冷性的关系密切(r=0.853**,r=0.915**),发现花粉大小与耐冷性之间有显著的正相关关系(r=0.75**),可育花粉数是水稻孕穗期耐冷性的主要构成因子。 相似文献
36.
To fill in the gaps in knowledge as to how individual sea cucumber Apostichopus japonicus behave consuming eukaryotic food sources in natural environments, eukaryotic communities in the faeces of sea cucumbers and sediments were analysed through one whole year based on 16S rRNA gene sequencing of the organelle genomes. A total of 390 eukaryotic features were obtained, and 99.7% of the features were assigned to chloroplasts. The eukaryotic communities in faeces and sediments showed seasonal fluctuations through one whole year based on Bray‐Curtis distance and community composition. Comparison of eukaryotic communities between faeces and sediments showed that 12 families including Chaetocerotaceae and Laminariaceae were more abundant in faeces than in sediments, suggesting that sea cucumbers may choose sediment containing these algal taxa more often compared with others in natural environments. All features of Laminariaceae were assigned to Saccharina japonica, which is consistent with the fact that this alga is one of the most suitable diets in the aquaculture of A. japonicus. Assessments of individual 16S amplicon sequences of both faecal and sediment samples could be an alternative tool to help us understand dynamic feeding behaviours of sea cucumber populations in contributing to bioresource conservation and development of a superior approach to aquaculture. 相似文献
37.
Satoko KANEMATSU Nobuhiro MINAKA Takao KOBAYASHI Akira KUDO Yoshihiro OHTSU 《Journal of General Plant Pathology》2000,66(3):191-201
Sequences of the ribosomal DNA (rDNA) internal transcribed spacer (ITS) regions were examined to infer a molecular phylogeny
of small-spored Phomopsis isolates, designated W-type (mainly white colony, weakly virulent, bearing both alpha and beta conidia at 25°C on PDA) and
G-type (mainly gray colony, highly virulent, bearing only alpha conidia at 25°C on PDA), and P. amygdali from fruit trees. Phomopsis G-type and P. amygdali were a monophyletic group distinct from the W-type. The W-type isolates were divided into two monophyletic groups. Diaporthe citri, D. tanakae, P. asparagi, P. viticola, P. vitimegaspora and D. nomurai, which are morphologically distinguishable from W- and G-types, differed from the W- and G-types in molecular phylogenetic
analyses. PCR-RFLP analysis of rDNA ITS regions was useful to distinguish each of the Phomopsis species and groups using three restriction enzymes. In mating tests, W-type isolates from fruit trees were heterothallic and
inter-fertile even between isolates belonging to the different monophyletic groups. Isolates of the G-type and P. amygdali collected in Japan were cross-fertile. Some isolates from Lunaria annua, Ulmus glabra and Juglans regia belonged to one of the two monophyletic groups of the W-type and were cross-fertile with W-type isolates from Rosaceous fruit
trees.
Received 27 September 1999/ Accepted in revised form 27 January 2000 相似文献
38.
Ayano KUDO Sho YOSHIMOTO Hiromitsu YOSHIDA Yusuke IZUMI Satoshi TAKAGI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):784
Cancer-associated fibroblasts (CAFs) play an essential role in tumor invasion and metastasis. In dogs, the biological features of CAFs have not been well characterized. The purpose of this study was to investigate differences in the biological activities of canine CAFs and normal fibroblasts (NFs), and their influence on the migration and invasion of cancer cells. Canine CAFs and NFs were harvested from surgically-resected malignant epithelial tumor tissues and skin tissues of dogs. A wound-healing assay was conducted to compare the migratory and invasive abilities of canine CAFs and NFs. The results of this study showed that canine CAFs have a greater migratory and invasive ability than NFs. To observe the indirect and direct interactions between fibroblasts and cancer cells, Boyden chamber assay and 3D co-culture with collagen gel were conducted. The number of migrated and infiltrated cancer cells co-cultured with canine CAFs was greater than that with NFs. In the 3D co-culture, cancer cells showed noteworthy proliferation on the surface of gels containing canine CAFs and invasion into the gel. On the other hand, no infiltration of cancer cells into the gel containing NFs was observed. It was suggested that canine CAFs activate migration and invasion of cancer cells and promote the infiltration of cancer cells into collagen gels. 相似文献
39.
A possible function of Nik-related kinase in the labyrinth layer of delayed delivery mouse placentas
Hiroshi YOMOGITA Hikaru ITO Kento HASHIMOTO Akihiko KUDO Toshiaki FUKUSHIMA Tsutomu ENDO Yoshikazu HIRATE Yoshihiro AKIMOTO Masayuki KOMADA Yoshiakira KANAI Naoyuki MIYASAKA Masami KANAI-AZUMA 《The Journal of reproduction and development》2023,69(1):32
In mice and humans, Nik-related protein kinase (Nrk) is an X-linked gene that encodes a serine/threonine kinase belonging to GCK group 4. Nrk knockout (Nrk KO) mice exhibit delayed delivery, possibly due to defective communication between the Nrk KO conceptus and its mother. However, the mechanism of delayed labor remains largely unknown. Here, we found that in pregnant mothers with the Nrk KO conceptus, the serum progesterone (P4) and placental lactogen (PL-2) concentrations in late pregnancy were higher than those in the wild type. Moreover, we demonstrated that Nrk is expressed in trophoblast giant cells (TGCs) and syncytiotrophoblast-2 (SynT-2) in the labyrinth layer of the mouse placenta. In the human placenta, NRK is also expressed in Syn-T in villi. Both human Syn-T and mouse TGCs of the labyrinth layer are present within fetal tissues that are in direct contact with the maternal blood. The labyrinth layer of the Nrk KO conceptus was gigantic, with enlarged cytoplasm and Golgi bodies in the TGCs. To investigate the function of Nrk in the labyrinth layer, a differentially expressed gene (DEG) analysis was performed. The DEG analysis revealed that labor-promoting factors, such as prostaglandins, were decreased, and pregnancy-maintaining factors, such as the prolactin family and P4 receptor, were increased. These findings suggest that the Nrk KO mice exhibit delayed delivery owing to high P4 concentrations caused by the hypersecretion of pregnancy-maintaining factors, such as PL-2, from the placenta. 相似文献
40.
Kawasumi T Kudo T Une Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):673-675
An adult male Japanese giant salamander (Andrias japonicus) died accidentally, and necropsy showed a white mass (23 × 15 mm) in the left kidney and hepatorrhexis with hemoperitoneum. Histologically, the renal mass was mainly composed of immature nephroblastic tumor cells. In the tumor tissue, a trabecular pattern lined by oval to polygonal tumor cells with a rich interstitium, solid growth and a few tubular structures was observed. Nephroblastic tumor cells were strongly positive for vimentin and weakly positive, and epithelium-like tumor cells were strongly positive for cytokeratin. However, antibody for Wilms' tumor protein 1 did not react with the salamander's cells. On electron microscopy, a desmosome junction was observed between tumor cells. This is the first report of nephroblastoma in a Japanese giant salamander. 相似文献