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81.
Yoshihisa OHTANI Tatsuyuki TAKAHASHI Katsuyoshi SATO Astrid ARDIYANTI Sang‐Houn SONG Reiichiro SATO Ken ONDA Yasunori WADA Yoshiaki OBARA Keiichi SUZUKI Akihiko HAGINO Sang‐gun ROH Kazuo KATOH 《Animal Science Journal》2012,83(12):788-795
Although our previous report demonstrated that adiponectin and AdipoR1 gene expressions changed among different lactation stages in the bovine mammary gland, its in vivo kinetics remain unclear in ruminant animals. In this study, we investigated the changes in circulating concentrations of adiponectin, as well as other metabolic hormones and metabolites, (i) during the periparturient period and (ii) among different lactation stages, in Holstein dairy cows. In experiment 1, serum adiponectin concentrations increased after parturition. Serum insulin concentrations were lower in the postpartum than prepartum period, whereas serum growth hormone (GH) concentrations increased in the postpartum period. Serum nonesterified fatty acids (NEFA) levels were increased during the postpartum period and were dependent on the parity. In experiment 2, there was no significant difference in plasma adiponectin concentrations among lactational stages. Plasma insulin concentrations tended to be lower in early lactation while plasma GH levels tended to be higher. Plasma NEFA concentrations were significantly lower in mid‐ and late‐lactation stages than non‐lactation stages. These findings indicate that elevation of serum adiponectin might be involved in energy metabolism just around parturition, and might exert its action through regulation of receptor expression levels in target tissues in each lactational stage in Holstein dairy cows. 相似文献
82.
Sang-gun ROH Yeon-hee HONG Daisuke HISHIKAWA Hiroaki TSUZUKI Hisae MIYAHARA Yukihiko NISHIMURA Chizu GOTO Yasuki SUZUKI Ki-choon CHOI Hong-gu LEE Shinichi SASAKI 《Animal Science Journal》2005,76(4):381-386
Ghrelin and growth hormone secretagogues receptor (GHS‐R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS‐R in adipocytes, the effect of the GHS‐R antagonist, [D‐Lys‐3]‐GHRP‐6 (His‐D‐Trp‐D‐Lys‐Trp‐D‐Phe‐Lys‐NH2), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D‐Lys‐3]‐GHRP‐6 (10?7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)‐γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D‐Lys‐3]‐GHRP‐6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol‐3‐phosphate dehydrogenase and upregulation of PPAR‐γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D‐Lys‐3]‐GHRP‐6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS‐R in adipogenesis can be blocked by treatment with [D‐Lys‐3]‐GHRP‐6. 相似文献
83.
Masashi KUMAGAI Keiichi SUZUKI Hisashi SHINOHARA Yukiko OHTOMO Akira NISHIDA 《Animal Science Journal》2005,76(5):407-412
Data on 1410 heavy racehorses were taken from the Hokkaido Banei Horse Racing Result Book published during 1997–2000. Heritabilities were estimated using the restricted maximum likelihood method with a full data set of the accumulated prize winnings and the frequency of medical treatment against digestive diseases. Heritability estimates for the same traits were also obtained using a partial data set from affected animals only. In addition, a ‘two classes, one threshold’ model was applied to the full data set for the heritability estimation of liability to the diseases. Heritabilities estimated by restricted maximum likelihood with the full data set were quite low: 0.09 ± 0.02 for the prize winnings and 0.08 ± 0.03 for all three digestive diseases – colic resulting from flatulence, constipation and overfeeding. Low heritabilities were also estimated for each of the three kinds of colic. Medium heritabilities were estimated for colic resulting from constipation (0.30 ± 0.19) and overfeeding (0.46 ± 0.22) using the partial data set taken from the affected animals. Using the threshold model, higher heritabilities from 0.42 ± 0.04 to 0.57 ± 0.03 were estimated for the liabilities to all three digestive diseases and to the individual digestive diseases. These results suggest that liability evaluation of the frequency of medical treatment is insufficient because all non‐affected animals are scored as 0. The high heritabilities estimated by the threshold model with half‐sib relationships should be verified in future studies by comparing them with estimates from other genetic relationships and with realized heritabilities from a small‐scale practical selection program. 相似文献
84.
Akinori Kiba Takako Ohgawara Kazuhiro Toyoda Miho Inoue-Ozaki Tadahiro Takeda Uppalapati Srinivasa Rao Toshiaki Kato Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2006,72(4):228-237
In the plant cell wall of Pisum sativum seedlings, we found an NTPase (E.C. 3.6.1.5.) with ATP-hydrolyzing activity that was regulated by an elicitor and suppressors
of defense from pea pathogen Mycosphaerella pinodes. The ATPase-rich fraction was purified from pea cell walls by NaCl solubilization, ammonium sulfate precipitation, and chromatography
with an ATP-conjugated agarose column and an anion-exchange column. The specific activity of the final ATPase-rich fraction
increased 600-fold over that of the initial NaCl-solubilized fraction. The purified ATPase-rich fraction also had peroxidase
activity and generated superoxide, both of which were regulated by the M. pinodes elicitor and suppressor (supprescins). Active staining and Western blot analysis also showed that the ATPase was copurified
along with peroxidases. In this fraction, a biotinylated elicitor and the supprescins were bound primarily and specifically
to ca. 55-kDa protein (CWP-55) with an N-terminal amino acid sequence of QEEISSYAVVFDA. The cDNA clone of CWP-55 contained five ACR domains, which are conserved in
the apyrases (NTPases), and the protein is identical to a pea NTPase cDNA (GenBank accession AB071369). Based on these results, we discuss a role for the plant cell wall in recognizing exogenous
signal molecules. 相似文献
85.
Hiroyuki Takahara Kazuhiro Toyoda Gento Tsuji Yasuyuki Kubo Yoshishige Inagaki Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2005,71(3):190-195
Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection. 相似文献
86.
Ayumi SUZUKI Satoko KUSUMOTO Hiromichi HORIE Yuichi TAKIKAWA 《Journal of General Plant Pathology》2002,68(4):398-400
A bacterial leaf spot disease was observed on Hedera helix (English ivy) and H. canariensis (Algerian ivy) in Japan. The causal agent was identified as Xanthomonas campestris pv. hederae (Arnaud 1920) Dye 1978.
Received 13 May 2002/ Accepted in revised form 3 July 2002 相似文献
87.
Ohba Y Iguchi T Hirose Y Takasu M Nishii N Maeda S Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):829-831
A 24-day-old female Holstein calf had a soft, painless fluctuating swelling on the median plane in the frontal region, but did not show any clinical symptoms including neurological signs. Computer tomography (CT) distinctly showed the cyst filled with fluid and part of the encephalon. Hence, this swelling was diagnosed as meningoencephalocele, but not meningocele. The meningoencephalocele was successfully repaired surgically. Meningoencephalocele can thus be easily recognised by CT in a calf. 相似文献
88.
Naoki ISOBE Tomoyasu KUROSE Naoki SUZUKI Tomoko KOSHIISHI Kazutoshi UENO Keiichi HISAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):59
The present study was undertaken to examine whether oral administration of colostrum to mastitic cows reduced inflammation in the udder. Fifty milliliters of a colostrum whey product was administered orally daily for 3 days to cows suffering from mastitis. Milk was collected on day 0 and 7 of colostrum administration. For Experiment 1, milk from 11 udder quarters with high somatic cell counts (SCC) in four cows was used. SCC in milk decreased significantly after colostrum administration, whereas colostrum administration increased sodium and IgA concentrations significantly compared with those before administration. In Experiment 2, cows with clinical mastitis were divided into two groups, with and without colostrum administration, whereas all cows with subclinical mastitis were administered colostrum. Antibiotics were infused into the mammary gland from the first day of colostrum administration for 2–4 days. There was no significant decrease in SCC after colostrum administration in any group. However, udder firmness in both clinical mastitis groups was reduced after administration regardless of colostrum administration. IgA concentration in both clinical mastitis groups was significantly increased after colostrum administration compared to that before administration, although there was no significant difference between them. These results suggest the possibility that oral administration of colostrum attenuates inflammation of the mammary gland. Further studies are required to examine the effect of colostrum more precisely using cows with subclinical and chronic mastitis and longer duration of colostrum administration. 相似文献
89.
Kenji TSUKANO Yasunobu NISHI Naoki SASAKI Kazuyuki SUZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1521
Very few epidemiologic studies have verified the utility of the right paramedian abomasopexy (RPA) technique in cows with right abomasal disorders. This study aimed to investigate the outcomes and risk factors for non-survival in the herd within 30 days of surgery in cows with right abomasal disorders who underwent the RPA technique. Forty-seven Holstein cows with right abomasal disorders (25 with right abomasal displacement [RDA] and 22 with right abomasal volvulus [RAV]) were included. Twenty-two cows with RDA (22/25, 88.0%) and 10 cows with RAV (10/22, 45.5%) survived at 30 days post-surgery. Multivariate logistic regression analysis indicated that hyponatremia, hypokalemia, and the presence of abomasal volvulus were the major risk factors associated with non-survival. 相似文献
90.
MICHIO AOYAMA HIROHITO GOTO HITOMI KAMIYA IKUO KANEKO SATOSHI KAWAE HIROKI KODAMA YASUSHI KONISHI KENN-ICHI KUSUMOTO HISATO MIURA EIICHI MORIYAMA KIYOSHI MURAKAMI TOSHIYA NAKANO FUTOSHI NOZAKI DAISUKE SASANO TAKAO SHIMIZU HIROKI SUZUKI YASUSHI TAKATSUKI AKITO TORIYAMA 《Fisheries Oceanography》2008,17(3):206-218
Impact of climate change on marine biogeochemical parameters and ecosystem is one of the important issues of our environment. Direct evidence of marine pelagic ecosystem changes is found with warming of sea water and sea‐level rise in the main stream of the Kuroshio in the East China Sea and the western North Pacific during these three decades based on the analysis of long‐term comprehensive hydrographic observations. In terms of annual mean, the warming rate of surface air temperature and sea surface temperature ranged from 0.15 to 0.21°C per decade in and around the main stream of the Kuroshio in the East China Sea, which exceed the global mean warming rate of 0.128 ± 0.026°C per decade during the period from 1956 to 2005 reported in IPCC 2007. One of the features in this rapid warming region is an increase of number of Pterosagitta draco, a cosmopolitan warm‐water zooplankton. Biogeochemical parameters, such as wet weight of zooplankton, plant pigment and nutrients concentration in the upper 200 m have been decreasing while dissolved oxygen content and seawater temperature have been increasing in the upper 200 m in the main stream of the Kuroshio in the East China Sea. These observed linear trends of the biogeochemical parameters would be foresights for temperate oceans in the future. 相似文献