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21.
Kazuyuki SUZUKI Toshio SHIMAMORI Ayano SATO Kenji TSUKANO Masakazu TSUCHIYA Jeffrey LAKRITZ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):977-979
The aim of the present study was to compare the ability of the commercially available portable test system (PTSTM) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTSTM was not significantly different from that of the traditional LAL-based assays. The results using PTSTM correlated with those using KT (r2=0.963, P<0.001) or KC assays (r2=0.982, P<0.001). Based on these findings, the PTSTM could be applied as a simplified system to assess endotoxin activity in bovine serum. 相似文献
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Ryota MATSUYAMA Naotoshi KUNINAGA Tomoya MORIMOTO Tetsuya SHIBANO Akiko SUDO Kazunari SUDO Makoto ASANO Masatsugu SUZUKI Tetsuo ASAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1179-1181
Plesiomonas shigelloides is a causal agent of gastroenteritis, sepsis
and meningitis in humans. We examined the prevalence of P. shigelloides
among great cormorants (Phalacrocorax carbo hanedae) in Japan and the
antimicrobial susceptibility of isolates. P. shigelloides was isolated
from 33 (47.8%) of 69 fecal samples from great cormorants in 2014. All 33 isolates were
subjected to antimicrobial susceptibility testing using broth microdilution methods, which
showed resistance to ampicillin (31 isolates, 93.9%), tetracycline (two isolates, 6.1%)
and trimethoprim (one isolate, 3.0%). The high prevalence of P.
shigelloides in the great cormorants implicates the possible microbiological
risk to public health. 相似文献
24.
Yojiro YANAGAWA Yukiko MATSUURA Masatsugu SUZUKI Shin-ichi SAGA Hideto OKUYAMA Daisuke FUKUI Gen BANDO Masashi NAGANO Seiji KATAGIRI Yoshiyuki TAKAHASHI Toshio TSUBOTA 《The Journal of reproduction and development》2015,61(1):61-66
Generally, sika deer conceive a single fetus, but approximately 80% of pregnant females have two corpora lutea (CLs). The function of the accessory CL (ACL) is unknown; moreover, the process of ACL formation is unclear, and understanding this is necessary to know its role. To elucidate the process of ACL formation, the ovarian dynamics of six adult Hokkaido sika deer females were examined ultrasonographically together with peripheral estradiol-17β and progesterone concentrations. ACLs formed in three females that conceived at the first estrus of the breeding season, but not in those females that conceived at the second estrus. After copulation, postconception ovulation of the dominant follicle of the first wave is induced by an increase in estradiol-17β, which leads to formation of an ACL. A relatively low concentration of progesterone after the first estrus of the breeding season is considered to be responsible for the increase in estradiol-17β after copulation. 相似文献
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Takafumi SUZUKI Naohito NISHII Satoshi TAKASHIMA Tatsuya MATSUBARA Atsushi IWASAWA Hirofumi TAKEUCHI Kohei TAHARA Tatsuyuki HACHISU Hitoshi KITAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1379-1383
Polyclonal immunoglobulin (Ig) G autoantibodies against insulin have been
identified in sera of healthy cats. We purified and fractionated insulin-binding IgGs from
cat sera by affinity chromatography and analyzed affinity of insulin-binding IgGs for
insulin and their epitopes. Following the passing of fraction A, which did not bind to
insulin, insulin-binding IgGs were eluted into two fractions, B and C, by affinity
chromatography using a column fixed with bovine insulin. Dissociation constant (KD) values
between insulin-binding IgGs and insulin, determined by surface plasmon resonance analysis
(Biacore™system), were 1.64e−4 M for fraction B (low affinity IgGs) and
2e−5 M for fraction C (high affinity IgGs). Epitope analysis was conducted
using 16 peptide fragments synthesized in concord with the amino acid sequence of feline
insulin by an enzyme-linked immunosorbent assay. Fractions B and C showed higher
absorbance (affinity) of the peptide fragment of 10 amino acid residues at the
carboxyl-terminal of the B chain (peptide No. 19), followed by peptide fragments of 6 to
15 amino acid residues of the B chain (peptide No. 8). Fraction C showed a higher
absorbance to 7 to 16 amino acid residues of the B chain (peptide No. 5) compared with the
absorbance of fraction B. Polyclonal insulin-binding IgGs may form a macromolecule complex
with insulin through the multiple affinity sites of IgG molecules. Feline insulin-binding
IgGs are multifocal and may be composed of multiple IgG components and insulin. 相似文献
26.
Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
Masatoshi OOGA Masataka G. SUZUKI Fugaku AOKI 《The Journal of reproduction and development》2015,61(3):179-184
Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplantation development. Based on immunocytochemical analysis using an anti-H2Bub1 antibody, no H2Bub1 signal was detected in the metaphase chromosomes of unfertilized oocytes or the pronuclei of early 1-cell stage embryos, but a weak signal was observed in late 1-cell stage embryos. The signal increased after cleavage into the 2-cell stage, and thereafter a strong signal was observed until the blastocyst stage. To assess the significance of H2Bub1 in the regulation of preimplantation development, RNF20 (an H2B-specific ubiquitin E3 ligase) was knocked down using small interfering RNA (siRNAs). In embryos treated with siRNA, the levels of Rnf20 mRNA and H2Bub1 decreased
at the 4-cell and morula stages. Although these embryos developed normally until the morula stage, only one-third developed into the blastocyst stage. These results suggested that H2Bub1 is involved in the regulation of preimplantation development. 相似文献
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Utilization of free amino acids, yolk proteins and lipids in developing eggs and yolk-sac larvae of walleye pollock Theragra chalcogramma 总被引:3,自引:0,他引:3
Nobuyuki Ohkubo Sayumi Sawaguchi Tomonori Hamatsu Takahiro Matsubara 《Fisheries Science》2006,72(3):620-630
ABSTRACT: To elucidate the utilization of the major yolk nutrient stocks in eggs and larvae of walleye pollock Theragra chalcogramma , the contents of free amino acids (FAA), the major yolk protein (180 kDa lipovitellin originated from vitellogenin B in ovulated eggs: oLv B), and lipids were measured. Most eggs hatched 18 days after fertilization at 5°C, and all larvae absorbed almost all their yolk mass by 28 days. The total FAA content showed no change during the first 6 days, and then decreased to 28% of the initial level by 18 days. The oLv B contents, measured by an enzyme-linked immunosorbent assay using a specific antiserum against oLv B, gradually decreased from 6 to 18 days, followed by a rapid decline. The content of phospholipids (PL) and triacylglycerols (TG) showed no marked change until hatching, and then decreased until disappearance of yolk sac. From these results, it is proposed that there are two main periods for nutrient utilization in embryos and larvae of walleye pollock. In the first period, FAA was mainly utilized until 18 days after fertilization. Active utilization of oLv B and lipids (PL and TG) instead of FAA occurred during the second period from 18 to 28 days. 相似文献
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