全文获取类型
收费全文 | 16607篇 |
免费 | 14篇 |
专业分类
林业 | 3633篇 |
农学 | 1300篇 |
基础科学 | 138篇 |
2762篇 | |
综合类 | 718篇 |
农作物 | 2098篇 |
水产渔业 | 1791篇 |
畜牧兽医 | 1197篇 |
园艺 | 1115篇 |
植物保护 | 1869篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 21篇 |
2020年 | 4篇 |
2019年 | 6篇 |
2018年 | 2746篇 |
2017年 | 2708篇 |
2016年 | 1187篇 |
2015年 | 72篇 |
2014年 | 17篇 |
2013年 | 21篇 |
2012年 | 802篇 |
2011年 | 2137篇 |
2010年 | 2106篇 |
2009年 | 1262篇 |
2008年 | 1331篇 |
2007年 | 1584篇 |
2006年 | 44篇 |
2005年 | 109篇 |
2004年 | 107篇 |
2003年 | 154篇 |
2002年 | 72篇 |
2001年 | 11篇 |
2000年 | 42篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 12篇 |
1992年 | 9篇 |
1990年 | 1篇 |
1989年 | 5篇 |
1988年 | 11篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1981年 | 1篇 |
1977年 | 5篇 |
1972年 | 1篇 |
1969年 | 2篇 |
1968年 | 4篇 |
1967年 | 1篇 |
1966年 | 4篇 |
1965年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Hordeum chilense Roem. et Schult. is a diploid wild South American barley that contains genes of interest for cereal breeding, many of them
located on chromosome 1Hch. In the current study, two H. chilense-wheat addition lines with deletions in the 1Hch chromosome were used for sub-arm localization of five prolamin (glutenin and gliadin) loci and 33 EST-SSR marker loci on
chromosome 1Hch. The two sets of markers were distributed across five sub-arm chromosome regions. Three glutenin loci (Glu-H
ch
2, Glu-H
ch
3, Glu-H
ch
4) together with the gliadin locus Gli-H
ch
1 were located on the distal 20% of the 1HchS arm, whereas the glutenin locus Glu-H
ch
1 was on the proximal 88% region of 1HchL. Among 33 EST-SSR marker loci, 7 (21.2%) were on the 1HchS arm and, of them, 3 (9.1%) were on the distal 20% end and 4 (12.1%) on the proximal 80% region. The 26 loci (78.8%) on 1HchL were distributed across three different regions: 18 (78.8%) in the proximal 88%, 3 (9.1%) in the distal 12% and 5 (15.2%)
in a region less than 12% from the distal end. The deletions in the 1Hch chromosome added to the common wheat background were thus shown to be useful for determining the sub-arm location of EST-SSR
and prolamin loci. This could facilitate the identification of molecular markers linked to genes of agronomic interest and
the isolation of such genes for use in common wheat improvement. 相似文献
992.
This study is part of an effort to improve the quality of dried shiitake mushrooms (Lentinula edodes) in accordance with consumer preference. Results are presented of a trend survey that examined the preferences of consumers
using questionnaires combined with sensory evaluation. From the original preference (OP) survey, the distributions of OP divided
by age class (AC) were statistically equal to the results obtained in 2000. The medians of OP for teenagers and twenties were
“neutral” and “slightly like,” respectively. A firm preference change occurred between teenagers and those in their twenties.
It is empirically realized that sensory intensity (SI) scores that were too high or too low led to a low hedonic preference
(HP) score. The same tendency was seen for “neutralists” and “likers.” In addition, almost all distributions concerned with
HP had no significant difference between 2000 and 2005; for example, that of SI divided by AC. These results showed that the
preference for dried shiitake mushroom has been unaffected by the passage of the past 5 years. Dried shiitake mushrooms have
been used from ancient times and for many dishes; therefore, the overall preference appears to have remained unchanged. 相似文献
993.
The aim of this study was to determine changes in crystallinity and crystalline unit cell type of heat-treated Scots pine
(Pinus sylvestris L.) and Uludağ fir (Abies nordmanniana stev. subsp. bornmuelleriana Mattf.) wood samples by means of FT-IR spectroscopic method. Heat treatment was applied on the test samples in an oven at
three different temperatures (120, 150, and 180°C) and for two different periods of time (6 and 10 h) under atmospheric pressure.
It was designated that crystallinity of both Scots pine and Uludağ fir wood samples increased during heat treatment depending
on the duration. However, monoclinic structure in crystalline unit cells of Scots pine and Uludağ fir wood samples converted
to triclinic structure when heat treated. It was estimated that monoclinic structure was dominant in the crystalline unit
cell. It was established that the crystalline structure of Scots pine wood samples was more affected by heat treatment than
that of Uludağ fir wood samples. 相似文献
994.
Mohammad R. Sabzalian Aghafakhr Mirlohi Ghodratollah Saeidi Mohammad T. Rabbani 《Genetic Resources and Crop Evolution》2009,56(8):1057-1064
Wild species of safflower, Carthamus oxyacanthus Bieb., is highly crossable with cultivated species, C. tinctorius L. and could be directly exploited in broadening safflower gene pool and improving the crop for biotic and abiotic stress
environments. In this study, genetic diversity among accessions of C. oxyacanthus and their relationships with cultivated safflower were evaluated using agro-morphological traits and polymorphic inter-simple
sequence repeats (ISSR) markers. Significant variation was observed among accessions particularly for seeds per capitulum,
seed yield per plant, harvest index and capitula per plant. Cluster analysis based on agro-morphological traits classified
the wild accessions in two groups according to their geographical regions, and separated them from the cultivated genotypes.
ISSR marker also revealed a high genetic variation among the accessions, and cluster analysis based on this marker divided
genotypes into four groups, with cultivated ones in a separate clade. Genetic variation observed among the wild safflower
germplasm at the DNA level was higher than the agro-morphological traits, indicating that ISSR is an effective marker system
for detecting diversity among safflower genotypes and their genetic relationships. Accessions of C. oxyacanthus with high genetic relationship to cultivated species could be used for interspecific hybridization in breeding programs of
safflower. 相似文献
995.
Growth and ontogeny of digestive function were studied in pikeperch (Sander lucioperca) larvae weaned on artificial food at different ages. Three weaning treatments initiated respectively on day 9 (W9), day 15
(W15) or day 21 (W21) post-hatching (p.h.) were compared with a control group, fed Artemia nauplii from first feeding until the end of the rearing trial on day 36 p.h. The digestive enzyme activities and the ontogeny
of digestive structures were investigated using enzymatic assays and histological methods. Growth of pikeperch larvae was
significantly affected by precocious weaning. Pancreatic (trypsin and amylase) and intestinal (leucine-alanine peptidase,
leucine aminopeptidase N and alkaline phosphatase) enzyme activities were detected from hatching onwards, increased at the
moment of first feeding and then decreased. Pepsin secretion occurred at day 29 p. h. only, concurrently with the stomach
development and differentiation of gastric glands. In the early weaning group (W9) the maturation process of intestinal enterocytes
seems to be impaired and/or delayed and several signs of malnutrition were recorded. Except for alkaline phosphatase activity,
no differences in enzyme activities and development of digestive structures were observed among the control, W21, and W15
groups. Moreover, at the end of the experiment, no differences in proteolytic activities were observed among larvae from the
different treatments, indicating that, in surviving individuals, the digestive structures were properly developed and the
larvae had acquired an adult mode of digestion. Based on the artificial diet used, our results suggested that pikeperch larvae
can be weaned from day 15 p.h. without significant adverse effect on digestive capacities (except for alkaline phosphatase)
or development of digestive tract, while earlier weaning impaired the onset of the maturation processes of the digestive system,
both in terms of morphological structures and enzymatic activities. 相似文献
996.
Three species of wireworms (Coleoptera: Elateridae) known to cause severe damage to potatoes in Europe and Asia have been introduced to Canada and are now well established as pests in the westernmost province of British Columbia (BC) (Agriotes obscurus and A. lineatus) and the eastern provinces of Nova Scotia and Prince Edward Island (A. obscurus, A. lineatus and A. sputator). Conventional insecticide-based efforts to control these invasive pests have had serious environmental impacts, or are failing to prevent severe economic damage from occurring to potatoes in some key potato production areas. Research toward developing an IPM programme for these exotic species has been completed or is underway in Canada, including the following: biological and ecological studies, development of monitoring and risk assessment programmes, and development of insecticidal and alternative control strategies. This research is summarized and implications for wireworm management in Europe are discussed. In addition to wireworms, one of the primary economic insect pests of potatoes in BC is the tuber flea beetle, Epitrix tuberis (Coleoptera: Chrysomelidae). The larvae of this beetle feed on developing or mature daughter tubers, producing surface channels or tracks and holes directly into the tuber. In the past, growers would apply 7–10 foliar sprays of broad-spectrum insecticides per growing season, which was not always successful in controlling this pest. In the EU, a newly identified flea beetle, Epitrix papa sp. n., as well as the North American species, E. cucumeris, have been identified as attacking potatoes initially in Portugal (2004) and later also in Spain. The potential spread of these emergent pests to various EU and non-EU countries is of concern, and if established would require the development of management strategies. The former development of a highly effective IPM programme developed in BC for E. tuberis is discussed, as is its potential for E. papa and E. cucumeris management in Europe. 相似文献
997.
Arben Q. Alla J. Julio Camarero Pilar Rivera Gabriel Montserrat-Martí 《Annals of Forest Science》2011,68(7):1245-1254
• Introduction and statement of the research questions
The relationships between primary and secondary growth in tree populations of contrasting climates are poorly understood. We tested the hypotheses that bud size and stem cross-sectional area are related through allometric relationship in shoots and that their scaling slopes change in response to climatic stress. 相似文献998.
Yang Yang Yuezhi Pan Xun Gong Moutian Fan 《Genetic Resources and Crop Evolution》2010,57(8):1239-1248
Citrus hongheensis is a critically endangered species endemic to the Honghe river region in southeastern Yunnan, China. Its genetic diversity
and differentiation were investigated using Inter-Simple Sequence Repeat (ISSR) markers. One hundred primers were screened,
and a total of 245 loci were amplified from seven natural populations by 13 informative and reliable primers. Of these 245
ISSR loci, 233 were polymorphic and the detected variations revealed a relatively high level of intraspecific genetic diversity.
At the population level, the mean percentage of polymorphic loci (PPB) was 36.50%, while the average expected heterozygosity (He) and Shannon diversity index (Ho) were 0.1327 and 0.1972, respectively. At the species level (across all populations), PPB was 95.10%, while He and Ho were 0.3520 and 0.5195, respectively. A high Gst value (0.6247) indicated that there is significant differentiation among populations, which was confirmed by AMOVA analysis
(Φst = 0.6420). Pairwise genetic identity (I) values among populations ranged from 0.6341 to 0.7675, with a mean of 0.7008. We propose that the high level of genetic
differentiation may be the result of habitat fragmentation and limited gene flow (Nm = 0.1502). For effective in situ conservation and population restoration of C. hongheensis it will be important to maintain historical processes, including high outbreeding rates, sufficient gene flow, and large
effective population sizes. 相似文献
999.
Carlos A. Guerra Marc J. Metzger Joachim Maes Teresa Pinto-Correia 《Landscape Ecology》2016,31(2):271-290
Context
Policy decisions form a major driver of land use change, with important implications for socially and environmentally susceptible regions. It is well known that there can be major unintended consequences, especially where policies are not tailored to regionally specific contexts.Objectives
In this paper we assess the implications of 60 years of agricultural policies on soil erosion prevention (SEP) by vegetation, an essential regulating ecosystem service in Mediterranean Europe.Methods
To assess these implications we produced and analysed a time series of land cover/use and environmental conditions datasets (from 1951 to 2012) in relation to changing agricultural policies for a specific region in the southern Portugal. A set of indicators related to SEP allowed us to identify that land use intensification as increased soil erosion in the last 60 years.Results
Particularly in the last 35 years, as a consequence of headage payments for cattle, the agricultural policy had a significant effect in the density and renewal of the tree cover, resulting in drastic effects for the provision of the SEP service. These are more significant after 1986, coinciding with the implementation of several Common Agricultural Policy instruments focused on increasing the modernization and productivity capacity of farm systems.Conclusions
The results show some unintended effects of agricultural policy mechanisms on ecosystem service provision and highlight the need for context-based policies, tailored to the environmental constrains and potentials of each region.1000.
Cinzia Calcabrini Roberta De Bellis Umberto Mancini Luigi Cucchiarini Vilberto Stocchi Lucia Potenza 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(2):192-197
Walnuts (Juglans regia L.) are relevant components of the Mediterranean diet providing important macronutrients, micronutrients and other bioactive constituents including unsaturated fatty acids, proteins, fiber, vitamins, minerals, phytosterols and polyphenols. Although the walnut beneficial effects in human health are widely recognized by a lot of epidemiologic studies very little is known regarding its effect on damaged DNA. The aim of the present study was to investigate the effect of Juglans regia L. ethanolic extract from kernel on the induction of DNA strand breaks by thiol/Fe3+/O2 mixed function oxidase, tert-butyl hydroperoxide or UVC radiations in acellular and cellular models. Plasmid DNA cleavage and fast Halo assay were used to monitor oxidative damage to DNA. Both approaches showed protection of oxidatively injured DNA. These results agree with a lot of scientific proofs which recommend walnut as dietary adjunct in health promotion and prevention as well as in treatment of lifestyle-related oxidative diseases. 相似文献