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71.
Tomonari Kasai Tomoko Suzuki Kozue Ono Ken'ichi Ogawa Yoshishige Inagaki Yuki Ichinose Kazuhiro Toyoda Tomonori Shiraishi 《Journal of General Plant Pathology》2006,72(5):265-272
We previously reported that the release of O2
− from isolated pea cell walls was enhanced by a 70-kDa glycoprotein elicitor but was suppressed by mucin-type glycopeptide
suppressors (supprescins A and B) prepared from pycnospore germination fluid of Mycosphaerella pinodes, causal agent of Mycosphaerella blight of pea. Here, we show that superoxide dismutase (SOD) in the apoplast fluid/cell wall
of pea seedlings responds to the fungal elicitor and suppressor molecules. In a pharmacological study and with internal amino
acid sequencing, the apoplastic SOD in a pea cultivar Midoriusui was found to be a Cu/Zn type SOD. We cloned a full-length
cDNA of the Cu/Zn-SOD and designated it as PsCu/Zn-SOD1. An increase in PsCu/Zn-SOD1 mRNA and the PsCu/Zn-SOD1 protein was induced by treatment with the elicitor more intensively
than by wounding. Such induction by the elicitor or wounding, however, was inhibited by the concomitant presence of supprescins.
The SOD activity of recombinant PsCu/Zn-SOD1 was regulated directly by these signal molecules in a manner similar to their
effect on the SOD activity in the apoplastic fluid and in the cell wall-bound proteins. Based on these findings, we discuss
a role for PsCu/Zn-SOD1 in the pea defense response.
The nucleotide sequence data of PsCu/Zn-SOD1 reported are available in the DDBJ/EMBL/GenBank databases under accession number AB189165. 相似文献
72.
Takeshi Shinogi Tomoko Suzuki Takayuki Kurihara Yoshihiro Narusaka Pyoyun Park 《Journal of General Plant Pathology》2003,69(1):7-16
Reactive oxygen species (ROS) generation was examined in the interaction of Alternaria alternata Japanese pear pathotype and host plants using three methods: nitro blue tetrazolium (NBT) method for microscopic detection
of O2
−, diaminobenzidine (DAB) methods for microscopic detection of H2O2, and cerium chloride methods for ultrastructural detection of H2O2. ROS generation was detected by NBT and DAB methods at appressoria on leaves of susceptible cultivars and heat-shocked leaves
of resistant cultivars but not in leaves of resistant cultivars. Ultrastructural detection by the cerium chloride method identified
ROS generation at cell walls of appressoria and penetration pegs in susceptible, resistant leaves and heat-shocked leaves.
These differences in the ultrastructural and microscopic data in resistant areas were due to the restriction of ROS generation
in limited areas, the side facing the plant surface, of appressoria and penetration pegs. Therefore, ROS generation was apparently
induced regardless of the resistance or susceptibility of the cultivar with the difference being in the volumes generated.
After evaluating the pathological role of ROS generation in fungal structures, such generation was found to be associated
with early penetration of cell walls in pear plants. Additionally, ROS generation in plants was also found in degrading pectin
layers near infected hyphae and in plasma membrane modification sites in susceptible leaves but not in resistant leaves. ROS
generation in susceptible leaves might be accompanied with plasma membrane damage, although the role of ROS generation in
the pectin layers is not clear. ROS generation in both fungal and plant cells during their interaction was likely associated
with the expression of susceptibility.
Received: June 3, 2002 / Accepted: July 31, 2002 相似文献
73.
Ookawa K Mochizuki K Sugimoto Y Suzuki T Hashizume M Kitagawa T Matumoto T Ito C Yokogoshi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):49-54
Lipid extracted from the ovary of skipjack tuna by the method that we developed is rich in phospholipid-type docosahexaenoic acid. The ovary lipid of skipjack tuna (OLS) was studied for its anti-stress activity in male Wistar rats, focusing on stress-related blood components: recovery from stress was examined after application of water immersion restraint stress. As a result, serum corticosterone (CORT) secretion was inhibited and decreased rapidly after stress application in rats given OLS compared with control rats. As CORT acts as a glucocorticoid, non-esterified fatty acid (NEFA) is expected to increase by stress application. However, the concentration tended to be lower in rats given OLS than in control rats. With respect to OLS concentration, OLS increased serum dehydroepiandrosterone, secretion concentration-dependently. In addition, as with the recovery study, it tended to inhibit the increase in NEFA. These results indicate that OLS may have an anti-stress activity against acute stress. 相似文献
74.
75.
Kyoji Hagiwara Takahiko Higashi Kazunari Takahashi Naho Hara Hideyuki Aoki Naoyuki Miyazaki Qing-Yu Wang Yafeng Zhu Osamu Yatou Hiroshi Tanaka Toshihiro Omura 《Journal of General Plant Pathology》2006,72(3):186-189
Transencapsidation of the Rice gall dwarf virus (RGDV) inner core by the Rice dwarf virus (RDV) outer capsid P8 protein was examined in vitro and in planta. When RGDV core particles were incubated with an extract
from RDV P8-transgenic rice leaf tissue, RDV P8 encapsidated the RGDV core particles to form double-shelled virus-like particles
in vitro. In contrast, when RDV P8-transgenic rice plants were inoculated with RGDV, progeny RGDV particles contained RGDV
P8 but RDV P8 was not detectable in the virions. No significant differences were found in acquisition by the vector insects
and subsequent transmission rates between RGDV infecting nontransgenic rice plants and those infecting RDV P8-transgenic rice
plants. These results indicate that mechanisms of and/or requirements for interactions between P8 and the inner core particles
of phytoreoviruses differ between in vitro and in planta. 相似文献
76.
Masumi Yamagishi Motoyasu Otani Mariko Higashi Yoshimichi Fukuta Kiichi Fukui Takiko Shimada 《Euphytica》1998,103(2):227-234
Diallel analysis has revealed that anther culturability in rice (Oryza sativa L.) is a quantitative trait controlled by the
nuclear genome. Mapping of anther culturability is important to increase the efficiency for green plant regeneration from
microspores. In the previous study, we detected distorted segregation of RFLP markers in rice populations derived from the
anther culture of an F1 hybrid between a japonica cultivar ‘Nipponbare’ and an indica cultivar ‘Milyang 23’. To clarify the association between chromosomal
regions showing distorted segregation and anther culturability, the anther culturability of doubled haploid lines derived
from the same cross combination was examined, and the association between alleles of the RFLP markers, which exhibiting the
most distorted segregation on chromosomes 1, 3, 7, 10 and 11, and the anther culturability was evaluated. One region on chromosome
1 was found to control callus formation from microspores, and one region on chromosome 10 appeared to control the ratio of
green to albino regenerated plants. In both regions, the Nipponbare allele had positive effects. Three regions on chromosomes
3, 7 and 11, however, showed no significant effect on anther culturability.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
77.
Digestion of carp myofibrils at 30 degrees C in 0.5 M KCl medium with calcium ion generated unique 135 kDa heavy meromyosin (HMM). The HMM was not produced when digested at 10 degrees C. A further digestion of the 135 kD HMM isolated in the absence of calcium ion generated uniquely short subfragment-2 (S-2) with a size of 40 kDa (40 kDa S-2) together with subfragment-1 (S-1). The 40 kDa S-2 was identified by N- and C-end sequencing, and demonstrated to locate the amino end of the rod portion. The unfolding temperature for the 40 kDa S-2 was around 52 degrees C as studied by circular dichroism measurement. The same unfolding peak was also detected with the intact rod together with a large unfolding peak at around 36 degrees C coming from the rest of the rod portion, light meromyosin. The unfolding peak for the 40 kDa S-2 in myosin was a little lower (48 degrees C) than that in free form, suggesting the involvement of the head portion in the stability of the 40 kDa S-2 in the structure. 相似文献
78.
Shogo HIGAKI Hongyu DARHAN Chie SUZUKI Tomoko SUDA Reina SAKURAI Koji YOSHIOKA 《The Journal of reproduction and development》2021,67(1):67
We aimed to determine the effectiveness of estrus detection based on continuous measurements of the ventral tail base surface temperature (ST) with supervised machine learning in cattle. ST data were obtained through 51 estrus cycles on 11 female cattle (six Holsteins and five Japanese Blacks) using the tail-attached sensor. Three estrus detection models were constructed with the training data (n = 17) using machine learning techniques (random forest, artificial neural network, and support vector machine) based on 13 features extracted from sensing data (indicative of estrus-associated ST changes). Estrus detection abilities of the three models on test data (n = 34) were not statistically different among models in terms of sensitivity and precision (range 50.0% to 58.8% and 60.6% to 73.1%, respectively). The relatively poor performance of the models might indicate the difficulty of separating estrus-associated ST changes from estrus-independent fluctuations in ST. 相似文献
79.
Kohei NISHIKAKU Tatsuo NOGUCHI Satoshi MURAKAMI Yasushi TORII Tomoko KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):350
Bovine leukemia virus (BLV) is an important pathogen associated with enzootic bovine leukosis. In this study, we performed PCR and sequencing analysis to characterize BLVgp51 sequences from formalin-fixed paraffin-embedded (FFPE) specimens made from 1974 to 2000 and successfully obtained BLV proviral genome sequences from 94% of the analyzed samples. Furthermore, from these samples, we reconstructed eight full-length and nearly full-length BLVgp51 sequences. These sequences were classified as BLV genotype 1, implying that genotype1 has already been circulating in Japan since the 1970s. In our results, the proviral DNA was detected in the 1970s, 1980s, and 1990s in the same manner, indicating that the detection of BLV proviral genome depends on storage conditions rather than storage period. The sequences obtained in this study provide direct insights into BLV sequences before 2000, which serves as a good calibrator for inferring ancient BLV diversity. 相似文献
80.
Tomoko SUZUKI Takeshi SHINOGI Kazutoshi UNNO Yoshihiro NARUSAKA Pyoyun PARK 《Journal of General Plant Pathology》2002,68(4):267-276
This fluorescence and immunoelectron microscopic study showed that β-1,3-D-glucan accumulated only in leaves of a susceptible cultivar of Japanese pear after treatment with a host-specific toxin,
AK-toxin I, from Alternate, alternata Japanese pear pathotype. The positive fluorescent reaction of callose was detected only in aniline blue fluorochrome-stained
sections from toxin-treated leaves of the susceptible cultivar: positive sites were observed on cell walls of leaf cells.
The sites of callose deposition were probably consistent spatially with modified sites on the plasma membrane that were observed
only in the toxin-treated leaves of the susceptible cultivar. The toxin-induced modifications, identified as damage to the
plasma membrane, were characterized by invagination of the plasmalemma specifically at plasmodesmata and as the concomitant
accumulation of extracellular polysaccharides at the invaginated sites. A positive reaction to anti-β-1,3-D-glucan antibody was detected at the polysaccharides, Golgi vesicles, and trans-Golgi network (TGN) of toxin-treated leaves of the susceptible cultivar, but not at Golgi vesicles and TGN of water-treated
ones. The cis-, medial and trans-Golgi stacks of toxin-treated leaves of the susceptible cultivar were negative for the antibody. The results showed that
the polysaccharides, Golgi vesicles and TGN contained abundant β-1,3-D-glucan and that the glucan was transported from the Golgi apparatus via Golgi vesicles to the modified sites in cells of toxin-treated leaves of the susceptible cultivar.
Received 7 March 2002/ Accepted in revised form 10 June 2002 相似文献