我国西部地区水电开发问题探讨

水能资源是我国西部地区的优势资源，由于历史和政策的原因，开发利用程度相对较低，资源优势没有形成产业优势。在分析西部水能资源分布特点和开发现状的基础上，结合西部大开发和西电东送战略，提出目前水电产业存在的市场、政策和资金方面问题，探讨了加快西部水电开发的对策和建议。     

松嫩草原羊草与其主要伴生种竞争与共存研究

基于对松嫩草原优势物种羊草(Leymus chi-nensis)及其主要伴生种的空间分布格局、植物种间关系对割草的响应,以及羊草、芦苇(Phragmites communis)对营养和胁迫的适应性与种间关系的研究,探讨了植物竞争和共存的潜在机制。     

锦江鱼类资源现状与物种多样性分析

2013年8月—2016年8月对沅江水系支流锦江的鱼类资源现状进行了调查。通过向渔民购买、现场捕捞等方式,共调查野生鱼类43种,隶属于5目11科39属,其中鲤科鱼类25种,占总种类数的58.1%。调查到新记录种5种,分别为大鳞副泥鳅(Paramisgurnus dabryanus)、吻(Rhinogobio typus)、洞庭华鲮(Sinilabeo tungting)、云南盘(Discogobio yunnanensis)和大眼鳜(Siniperca kneri)。干、支流均以底层鱼类为主,种类数分别占各自总数的55.8%和71.4%。对比历史资料,G-F多样性指数呈下降趋势。对干流渔获物种类的周年统计发现,出现频率在50%以上的鱼类有8种,其中泥鳅(Misgurnus anguillicaudatus)、鲤(Cyprinus carpio)、鲫(Carassius auratus)和鲇(Silurus asotus)的出现频率均达到97.3%。     

高产高效栽培下春玉米生育后期冠层结构及其光合特性的研究

以郑单958为供试品种,设高产高效栽培(GCGX)和农户传统栽培(CK)2个处理,于2012年和2013年连续2 a进行田间试验,测定春玉米生育后期冠层结构及其光合特性的变化规律。结果表明：吐丝期和乳熟期株高、穗位高产高效栽培略高于农户传统栽培。与农户传统栽培相比,高产高效栽培春玉米叶面积指数较大,在生育期上表现为吐丝之后更为明显,在叶位上表现为棒三叶以下叶片最为突出;不同叶位的叶倾角高产高效栽培显著小于农户传统栽培,而叶向值均显著大于农户传统栽培,在棒三叶表现最为明显;高产高效栽培生育后期净光合速率显著高于农户传统栽培,冠层光合能力极显著高于农户传统栽培。高产高效栽培下春玉米产量为14.009 t·hm^-2(2012)和13.255 t·hm^-2(2013),均极显著高于农户传统栽培。     

The Influence of Oxygen Tension on Cumulus Cell Viability of Canine COCs Matured in High-Glucose Medium

High in vitro oxygen (O₂) tensions are associated with enhanced levels of reactive oxygen species and cumulus oocyte complex (COC) apoptosis. The objective of this study was to determine the influence of O₂ tension on cumulus cell (CC) viability from canine oocytes. Cumulus oocyte complexes were distributed into three groups (CG, T20 and T5) and two O₂ tension levels (20% and 5%). The control group (CG) was matured in vitro in a humidified atmosphere with 5% CO₂ in air in TCM199 with 26.19 m m sodium bicarbonate, 10% (v/v) foetal calf serum (FCS), 0.10 m m gentamicin, 0.20 m m pyruvic acid, 20 μg/ml oestradiol, 0.5 μg/ml follicle-stimulating hormone, 0.03 IU/ml human chorionic gonadotropin, and 1.0 μg/ml human somatotropin. Groups T20 and T5 were matured under 20% or 5% O₂ tensions respectively in a high-glucose medium, without FCS. T20 and T5 were as CG, and supplemented with 0.1% Polyvinyl Alcohol, and 5.5 m m glucose. After 48 h of IVM, CCs from COCs were stained with propidium iodide (1.50 m m ). The results showed that viability of CCs (cytoplasmic features and nuclear morphological integrity) was different for the three groups. Rates of apoptosis were at 57.9% (521/900) for CG, 54.4% (490/900) for T20 and 38.9% (350/900) for T5 (p < 0.001). Predominant features in apoptotic cells (n = 1361) were DNA nuclear fragments (94.0%). It was concluded that CCs of canine COCs cultured in high-glucose medium showed significantly less apoptosis than those cultured in medium with FCS. Low O₂ tension was efficient in reducing apoptosis in canine CCs.     

Meniscal Mineralization in Domestic Cats

Objective: To (1) determine prevalence of radiographically detectable meniscal mineralization in domestic cats and (2) to evaluate the association between meniscal mineralization and degenerative joint disease (DJD). Study Design: Prospective study. Animals: Client‐owned cats (n=100) and 30 feline cadavers. Methods: Randomly selected client‐owned cats were used to determine the prevalence of meniscal mineralization. Stifles from feline cadavers were used to evaluate the relationship between meniscal mineralization (using high‐resolution X‐ray), radiographic DJD, and cartilage damage. Menisci were evaluated histologically. Results: Forty‐six percent of the client‐owned cats had meniscal mineralization detected in 1 or both stifles. Pain scores were not significantly different between stifles with meniscal mineralization and those with no radiographic pathology (P=.38). Thirty‐four of 57 cadaver stifles had meniscal mineralization, which was always located in the cranial horn of the medial meniscus. Percentage mineralization of the menisci was significantly correlated with the cartilage damage score of the medial femoral (r²=0.6; P<.0001) and tibial (r²=0.5; P<.0001) condyles as well as with the total joint cartilage damage (r²=0.36; P<.0001) score and DJD score (r²=0.8; P<.0001). Conclusion: Meniscal mineralization is a common condition in domestic cats and seems to indicate medial compartment DJD. Clinical Relevance: Clinical significance of meniscal mineralization is uncertain. Further work is needed to determine if the meniscal mineralization is a cause, or a consequence of joint degeneration.     

玉屏风多糖脂质体的制备及表征研究

优选玉屏风多糖脂质体的最佳工艺并对其进行表征验证。采用逆向蒸发法制备玉屏风多糖脂质体,以膜材比、脂药比和超声时间为考察因素,包封率为评价指标,采用L9(34)正交试验设计优选制备条件,以超速离心法测定包封率,体外释药试验验证,纳米颗粒分析仪和透射电镜测定其粒径大小与形态。制备玉屏风多糖脂质体的最佳工艺为膜材比8∶1,脂药比4∶1,超声时间90s,包封率88.38%,24h累积释放率73.45%,平均粒径132.65nm。制备的玉屏风多糖脂质体具有较高的包封率与良好的缓释作用,粒径和形态较均匀,重现性好。     

钾营养对莲藕产量形成和氮磷钾养分累积分配的影响

【目的】研究钾营养对莲藕产量形成及氮磷钾养分积累与分配的影响。【方法】采用盆栽试验,设置不施钾（-K）和施钾（+K）处理进行全生育期试验。【结果】莲藕移栽76 d后,+K处理叶片和叶柄干物质累积量明显高于-K处理,并均在97 d膨大茎成型后逐渐降低;膨大茎干物质累积速率和累积量在早期为+K处理低于-K处理,97 d后+K处理的干物质累积速率明显加快,其累积量于139 d明显高于-K处理,160 d不同处理膨大茎干物质累积量趋于稳定,+K处理为1 204 g/株,比-K处理高19.7%。在97—160 d产量形成关键期,膨大茎中氮、磷和钾的累积量,+K处理比-K处理分别高32.8%、10.3%和74.2%,其中来自其它器官转移的氮、磷和钾,+K处理比-K处理分别高1.20、0.12和1.26 g/株,来自根系直接从土壤中吸收转运的氮、磷和钾,前者比后者高2.16、0.04和5.70 g/株。莲藕氮、磷、钾累积总量之比-K处理为1﹕0.14﹕0.90,+K处理为1﹕0.13﹕1.31。【结论】钾营养促进莲藕干物质生产和累积、促进根系对养分的吸收并提高莲藕各器官尤其是叶柄中钾的含量,在生育后期促进干物质和养分从叶片等器官转移并贮存到膨大茎中并最终增产。     

Omentum-Enhanced Reconstruction of Chronic Nonhealing Wounds in Cats: Techniques and Clinical Use

The greater omentum was used to aid in the reconstruction of nonhealing wounds in five cats. In each cat the cause of the nonhealing wound could not be determined. A vascular omental pedicle was created at laparotomy by dividing the dorsal leaf of the omentum at its pancreatic attachments (four cats) or by dividing the ventral leaf at its gastric attachments (one cat). The pedicle was then passed through either the ventral or lateral body wall into the nonhealing wound via a subcutaneous tunnel. The wounds were closed over the omentum after local tissue mobilization. All the wounds healed uneventfully and have remained healed with a mean follow-up period of 2.5 years. Complications included intestinal herniation through a ventral midline exit hole (one cat), presumed abdominal fat herniation through a ventral abdominal exit hole (one cat) and seroma formation (one cat). All of the complications were treated successfully. The omentum should be considered a useful adjunct in the treatment of nonhealing wounds in cats.