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71.
Tatiana da Rosa Guimar?es Carlos Guillermo Quiroz Caroline Rigotto Simone Quintana de Oliveira Maria Tereza Rojo de Almeida éverson Miguel Bianco Maria Izabel Goulart Moritz Jo?o Luís Carraro Jorge Alejandro Palermo Gabriela Cabrera Eloir Paulo Schenkel Flávio Henrique Reginatto Cláudia Maria Oliveira Sim?es 《Marine drugs》2013,11(11):4176-4192
The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2. 相似文献
72.
Schossler Thiago Rodrigo Mantovanelli Bruno Campos de Almeida Brivaldo Gomes Freire Fernando José da Silva Manassés Mesquita de Almeida Ceres Duarte Guedes Cabral Freire Maria Betânia Galvão dos Santos 《Precision Agriculture》2019,20(6):1274-1291
Precision Agriculture - Sugarcane productivity is highly variable across space, and knowledge about the spatial distribution of soil physical attributes is important to guide management practices... 相似文献
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74.
Gabriela Vargas Jos Verdejo Adrian Rivera Domingo Surez Cristian Youlton Juan L. Celis-Diez Yves Le Bissonnais Elvira A. Dovletyarova Alexander Neaman 《植物养料与土壤学杂志》2019,182(2):159-166
The structural stability of soil is a physical characteristic that affects soil degradation processes. Calcium‐based amendments, such as calcium carbonate, calcium sulfate, and calcium oxide/hydroxide, have been shown to improve the stability of soil aggregates. This study seeks to determine which calcium‐based soil amendments, and at what concentration, are the most efficient in improving aggregate stability of sandy topsoils derived from granitic and metamorphic parent materials, and to analyze the mechanisms involved. In the pot experiment, soils amended with CaCO3, CaCl2, and CaSO4 did not present significant differences in aggregate stability compared to the control or among each other. In contrast, Ca(OH)2 soil amendment brought the greatest stability to the soil aggregates. A dose of 1% Ca(OH)2 significantly increased the stability of soil aggregates. This effect is due to the reaction of Ca(OH)2 with atmospheric CO2 which leads to the formation of CaCO3, a delayed reaction not showed by the other soil amendments tested. Likewise, the greater solubility of Ca(OH)2 compared to CaCO3 exerts a greater aggregation effect on soil. Thus, the mechanism of action of Ca(OH)2 is related to cementation, rather than flocculation. Future studies should be carried out to demonstrate the effectiveness of Ca(OH)2 under field conditions. 相似文献
75.
Andr Z. Boaratti Thiago M. T. Nascimento Kifayat U. Khan Cleber F. M. Mansano Thaís S. Oliveira Daniel M. A. Queiroz Rafael S. Romaneli Andressa T. Rodrigues Nilva K. Sakomura Joo B. K. Fernandes 《Journal of the World Aquaculture Society》2021,52(1):88-104
The present study aimed to determine the ideal ratios of digestible essential amino acids (EAAs) for pacu (Piaractus mesopotamicus) juveniles by the amino acid (AA) deletion method. A completely randomized design which consisted of 11 treatments and three replicates each was used. The treatments included a control diet (CD) containing 55% of nonpurified natural ingredients and 45% of purified synthetic amino acids and ingredients, and other ten isonitrogenous and isoenergetic EAA limiting diets (LDs), each being deficient in 44.4 ± 0.02% of the respective EAA. Pacu juveniles with initial average body weight of 6.22 ± 0.09 g were distributed among 33 fiber glass tanks. Fish were fed with semipurified and extruded diets for 113 days two times a day until apparent satiation. The ideal ratio of each dietary EAA was calculated on the basis of the relationship between body N retention and amount of EAA deleted from the respective EAA LD. Based on the AA deletion method, the ideal ratios of digestible EAAs for pacu juveniles, relative to lysine requirement of 100% were estimated as: methionine 14.6%, threonine 35.0%, tryptophan 6.6%, arginine 62.8%, histidine 13.6%, isoleucine 26.3%, leucine 43.7%, phenylalanine 27.2%, and valine 35.8%. 相似文献
76.
Dinorah Vargas Lilia Gutierrez Ivan Juárez Francisco Gonzalez Héctor Sumano 《American journal of veterinary research》2008,69(8):1085-1090
OBJECTIVE: To determine the pharmacokinetics after SC administration of an experimental, long-acting parenteral formulation of doxycycline hyclate in a poloxamer-based matrix and after IV and IM administration of an aqueous formulation of doxycycline hyclate in goats. ANIMALS: 30 clinically normal adult goats. PROCEDURES: Goats were allocated to 3 groups (10 goats/group). One group of goats received doxycycline hyclate (10 mg/kg) IM, a second group received the same dosage of doxycycline hyclate IV, and the third group received the long-acting parenteral formulation of doxycycline hyclate SC. Serum concentrations of doxycycline were determined before and at various intervals after administration. RESULTS: The long-acting parenteral formulation of doxycycline hyclate had the greatest bioavailability (545%); mean +/- SD maximum serum concentration was 2.4 +/- 0.95 microg/mL, peak time to maximum concentration was 19.23 +/- 2.03 hours, and elimination half-life was 40.92 +/- 4.25 hours. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that the long-acting parenteral formulation of doxycycline hyclate distributed quickly and widely throughout the body after a single dose administered SC, and there was a prolonged half-life. Bioavailability of the longacting parenteral formulation of doxycycline hyclate after SC administration was excellent, compared with bioavailability after IV and IM administration of an aqueous formulation of doxycycline hyclate. Although no local tissue irritation and adverse effects were detected, clinical assessment of drug-residues and toxicologic evaluations are warranted before this long-acting parenteral formulation of doxycycline hyclate can be considered for use in goats with bacterial infections. 相似文献
77.
78.
Danilo V. G. Vieira Jos H. V. Silva Fernando G. P. Costa Thiago S. Melo Matheus R. Lima Danilo T. Cavalcante Jos G. de Vargas Júnior Talita P. Bonaparte Lorena C. Oliveira 《Journal of animal physiology and animal nutrition》2019,103(1):116-124
Experiment I: T1‐1 = basal diet with 25% crude protein (CP) + limiting amino acids (LA); T1‐2 = 20% CP + LA; T1‐3 = 20% CP + LA + L‐glycine; T1‐4 = 20% CP + LA + L‐glutamate; T1‐5 = 20% CP + LA + L‐glycine + L‐glutamate. Experiment II: T2‐1 = basal diet with 22% CP + LA; T2‐2 = 20% CP + LA; T2‐3 = 17.6% CP + LA + L‐glycine; T2‐4 = 17.6% CP + LA + L‐glutamate; T2‐5 = 17.6% CP + LA + L‐glycine + L‐glutamate. The reduction of dietary protein based on the concept of ideal protein decreases nitrogen excretion in quails when L‐glycine is added to the diets. Quails fed diets supplemented with L‐glutamate as the non‐specific nitrogen source equivalent to the nitrogen level of the control diet had increased nitrogen excretion. However, quails had reduced nitrogen excretion in both experiments when L‐glycine was added to diets with L‐glutamate. Carcass fat was increased by reducing dietary protein, but fat deposition was reduced by adding L‐glutamate and L‐glycine, or both. The dietary addition of L‐glutamate and L‐glycine in quails based on the ideal protein concept is not necessary (Exp. I). Although the total nitrogen, electrolytic balance and glycine level were adjusted in diets, quails had decreased performance. Therefore, other hypotheses besides protein reduction need to be studied (Exp. II). Protein reduction with supplementation of only limiting essential amino acids does not affect quail performance. Dietary addition of L‐glycine reduces nitrogen excretion. 相似文献
79.
Luiz Sergio Almeida Camargo Fernanda Queiros Costa Michele Munk Sabine Wohlres‐Viana Raquel Varela Serapio Bruno Campos Carvalho Paulo Henrique Campos Alex Cabral Vieira Luiz Altamiro Garcia Nogueira Joao Henrique Moreira Viana 《Reproduction in domestic animals》2019,54(10):1357-1365
This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus–oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleavage at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes. 相似文献
80.
da Silva José Ricardo Coelho de Carvalho Francisco Fernando Ramos de Andrade Fereira Marcelo de Souza Evaristo Jorge Oliveira Maciel Maria Inês Sucupira Barreto Lígia Maria Gomes Lopes Levi Auto Cordeiro Eduardo Henrique Araújo Véras Antonia Sherlânea Chaves 《Tropical animal health and production》2019,51(8):2455-2463
Tropical Animal Health and Production - The objective of this study was to evaluate carcass characteristics, meat quality, and organ and viscera weight of forty male sheep, not castrated, initially... 相似文献