全文获取类型
收费全文 | 213篇 |
免费 | 30篇 |
专业分类
林业 | 8篇 |
农学 | 14篇 |
39篇 | |
综合类 | 2篇 |
农作物 | 13篇 |
水产渔业 | 21篇 |
畜牧兽医 | 119篇 |
园艺 | 2篇 |
植物保护 | 25篇 |
出版年
2023年 | 5篇 |
2022年 | 4篇 |
2021年 | 16篇 |
2020年 | 9篇 |
2019年 | 14篇 |
2018年 | 14篇 |
2017年 | 18篇 |
2016年 | 17篇 |
2015年 | 10篇 |
2014年 | 23篇 |
2013年 | 12篇 |
2012年 | 20篇 |
2011年 | 23篇 |
2010年 | 5篇 |
2009年 | 4篇 |
2008年 | 6篇 |
2007年 | 8篇 |
2006年 | 7篇 |
2005年 | 2篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 5篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1990年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有243条查询结果,搜索用时 31 毫秒
41.
Castro Liliane Araújo Andrade-Porto Sanny Maria Oliveira Rebeca Guimarães Batista Yasmin Luzeiro Silva Felipe Moura Araújo Oliveira Christiane Patrícia Feitosa Affonso Elizabeth Gusmão Cruz Claudinei Tavares-Dias Marcos 《Aquaculture International》2021,29(6):2477-2488
Aquaculture International - This study investigated the efficacy of trichlorfon against Neoechinorhynchus buttnerae, as well as its palatability, residue analysis, and the blood parameters of... 相似文献
42.
Vanessa Ap R O Vieira Alexandre W S Hilsdorf Renata Guimarães Moreira 《Aquaculture Research》2012,43(4):565-576
Protein and lipid content as well as the fatty acid (FA) composition of storage tissues were analysed in two varieties of Oreochromis niloticus (Red‐Stirling and Chitralada) and their hybrid. The animals were maintained in cages for 11 months. The samples were taken when the animals weighed 10, 50, 100, 250 and 500 g. The results showed that changes in the metabolic processes occur during an increase in body mass in both varieties of tilapia and also their hybrid, but that these differences are not found in animals collected at the commercial weight. The protein content of the fillet and liver decreased with growth and the same protein content associated with growth was found for fillet lipid content. The genetic variety did not influence the FA profile of the fillet, but different genotypes had different hepatic FA compositions. Even with the same lipid content, the hepatocytes of Chitralada accumulated higher levels of polyunsaturated fatty acids (PUFA) n6 in triglycerides and increased C22:6n3 in the hepatocyte membranes. The higher n6PUFA content was compensated by a lower fraction of saturated FA in the hepatocyte triglycerides. The skin of Chitralada also had higher n6PUFA and C22:6n3 contents, suggesting a higher ability to deposit PUFA in the skin due to alterations in the liver synthetic pathway. 相似文献
43.
Shayne P. Bisetto Adriano B. Carregaro André E.S. Nicolai Thais F. Bressan William P. Leal Nathalia V. Xavier Diego F. Leal André F.C. Andrade 《Veterinary anaesthesia and analgesia》2017,44(3):594-599
Objective
To evaluate the effect of hyaluronidase on uptake, duration and speed of elimination of xylazine–tiletamine–zolazepam administered in the subcutaneous fat over the dorsal lumbar region of swine.Study design
Blinded, randomized, crossover study.Animals
Six healthy Landrace/Large White pigs weighing 132 ± 24 kg (mean ± standard deviation).Methods
Animals were administered xylazine (1 mg kg?1) and tiletamine–zolazepam (8 mg kg?1) (control treatment, CON), or xylazine–tiletamine–zolazepam at the same doses with hyaluronidase (400 IU) (treatment HYA). The treatments were administered into the dorsal lumbar adipose tissue, 2.5–3.0 cm laterally from the spinous process of the second lumbar vertebra. The latency, anesthesia and recovery periods were measured. Heart rate, noninvasive systolic, diastolic, and mean arterial pressures, respiratory rate, hemoglobin oxygen saturation and rectal temperature were recorded every 10 minutes for up to 50 minutes.Results
One animal in CON and one animal in HYA were responsive to stimulation and did not allow safe handling. No significant difference was found between treatments for latency (CON 11.3 ± 5.9 minutes, HYA 7.4 ± 5.1 minutes) and anesthesia (CON 53 ± 53 minutes, HYA 49 ± 38 minutes) periods. Recovery period was shorter in HYA (9 ± 6 minutes) than in CON (32 ± 16 minutes) (p < 0.05). Physiological variables were not significantly changed over time and were within accepted normal clinical limits for the species in both treatments.Conclusion and clinical relevance
Hyaluronidase (400 IU) administered into adipose tissue in pigs did not reduce the latency and duration of dissociative anesthesia, but was associated with faster recovery. 相似文献44.
Diogenes Dezen Franciscus Antonius Maria Rijsewijk Thais Fumaco Teixeira Carine Lidiane Holz Samuel Paulo Cibulski Ana Cláudia Franco Odir Antonio Dellagostin Paulo M. Roehe 《Research in veterinary science》2010,88(3):436-440
Multiply-primed rolling-circle amplification (MPRCA) was used to amplify porcine circovirus type 2 (PCV2) genomes isolated from tissues of pigs with signs of post-weaning multisystemic wasting syndrome (PMWS). Two of the amplified PCV2 genomes were cloned in prokaryotic plasmids and sequenced. Both were nearly identical (1767 nt) except for one silent substitution in the region coding for the capsid protein (ORF2). In addition, they showed high nucleotide sequence similarity with PCV2 isolates from others countries (93–99%). To investigate whether the MPRCA amplified PCV2 genomes could be used to produce infectious virus, the cloned genomes were isolated from the plasmids, recircularized and used for transfection in PK-15 cells. This procedure led to the production of infectious virus to titres up to 105.55 TCID50/mL. It was concluded that MPRCA is a useful tool to amplify PCV2 genomes aiming at sequencing and virus isolation strategies, where particularly useful is the fact that it allows straightforward construction of PCV2 infectious clones from amplified genomes. However, it was less sensitive than PCR for diagnostic purposes. 相似文献
45.
46.
47.
Enio Tarso de Souza Costa Luiz Roberto Guimarães Guilherme Guilherme Lopes Nilton Curi 《Water, air, and soil pollution》2012,223(4):1661-1670
Assessing multielement adsorption of trace metals on materials having potential to be used as soil amendments is an essential
stage for the remediation success, as soil contamination rarely occurs with a single element. This study evaluated mono-/multielement
adsorption of Zn, Cu, Cd, and Pb on aluminum (AMB) and iron mining by-products (IMB, used for comparison). Prior to adsorption,
these products were characterized by X-ray diffraction, isoelectric point, infrared spectroscopy, scanning electron microscopy,
and microwave furnace digestion. Sorption experiments comprised: (1) pH adjustment (5.5, 6.5, and natural suspension pH),
(2) mono- and multielement adsorption, and (3) desorption. Rising pH from 5.5 to natural suspension values (9.5) increased
monoelement adsorption of Zn, Cu, Cd, and Pb on AMB up to 3.8-, 1.4-, 6.2-, and 1.1-fold, whereas multielement adsorption
was increased up to 17.3-, 2.0-, 20.3-, and 1.2-fold, respectively. Zinc and Cd were less adsorbed than Cu and Pb and more
affected by competition. Multielement adsorption at 5.5 pH in AMB resulted in smaller adsorption of Zn (up to 4.6-fold), Cu
(1.4-fold), Cd (3.3-fold), and Pb (1.1-fold) when compared with monoelement adsorption. The lower the pH, the smaller the
adsorption and the higher the desorption. The AMB showed higher capacity to maintain the elements adsorbed than the IMB. 相似文献
48.
Camilo Allyson Simões de Farias Celso Augusto Guimarães Santos 《Journal of Soils and Sediments》2014,14(7):1242-1250
Purpose
We investigated the application of Kohonen Neural Networks (KNNs) in order to estimate sediment yield based on runoff and climatological data in a semiarid region of Brazil. Accurate estimations of sediment yield are essential to improve the management of soil erosion in semiarid areas, where large quantities of sediments tend to be produced only periodically.Materials and methods
The case study is an erosion plot within the São João do Cariri Experimental Basin, which is located in the semiarid portion of Paraíba State, Brazil. KNNs are unsupervised neural networks capable of reducing a multidimensional data set to a bidimensional matrix of features, which can be used for analysis and prediction purposes. A total of 60 rainfall events, which occurred between 1999 and 2002, were used to calibrate and test the model. The application of a multivariate linear regression (MLR) model was also carried out.Results and discussion
Statistical indexes were used as criteria for evaluating the performance of the KNN and MLR models for the test data set. The correlation and relative bias of the KNN model estimations with those from observed data were 0.90 and ?4.39 %, respectively. A correlation of 0.70 and a relative bias of 15.63 % were found from the comparison of sediment yields obtained by the MLR model with those of the observed data. Analysis of the outcomes indicates that the KNN model, which is capable of detecting and extracting nonlinear trends, produced more reliable results than the regression model.Conclusions
The KNN model results appear to be superior to those generated by the MLR model and suggest that the developed methodology may be applied to similar case studies. 相似文献49.
Serão NV Veroneze R Ribeiro AM Verardo LL Braccini Neto J Gasparino E Campos CF Lopes PS Guimarães SE 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2011,128(1):28-34
Seventy-two pigs of three genetic groups (Brazilian indigenous breed Piau, Commercial line and Crossbred) of both sexes were slaughtered at four live weights (30, 60, 90 and 120 kg). Intramuscular fat (IMF) content in Longissimus dorsi muscle of each animal was extracted and correlated with candidate gene mRNA expression (ATN1, EEF1A2, FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1). Within slaughter weight of 120 kg, Piau and Crossbred pigs showed higher IMF content (p < 0.05) than commercial animals, with 2.48, 2.08 and 1.00% respectively. Barrows presented higher values of IMF (p < 0.05) than gilts (1.54 and 1.30% respectively). Gene expression of EEF1A2, FABP3, LDLR, OBSCN, PDHB, TRDN and RYR1 were correlated with IMF (p < 0.05) using the whole dataset. For Piau data only, expression of FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1 showed correlation with IMF (p < 0.05). Genes that have important roles in lipid transportation inside the cell (FABP3) and tissues (LDLR) showed correlation with IMF of, respectively, 0.68 and 0.63 using the whole data set, and 0.90 and 0.91 using data from Piau animals. The highly positive correlation of the LDLR and FAPB3 expression with IMF content may confirm that these genes are important for fat deposition in the porcine L. dorsi muscle. 相似文献
50.
Andrade HM Toledo VP Pinheiro MB Guimarães TM Oliveira NC Castro JA Silva RN Amorim AC Brandão RM Yoko M Silva AS Dumont K Ribeiro ML Bartchewsky W Monte SJ 《Veterinary parasitology》2011,181(2-4):83-90
Dogs naturally infected with Leishmania Infantum (=L. chagasi) were treated with miltefosine using different therapeutic regimens. The animals were evaluated for clinical evolution, biochemical parameters, parasite load (by real-time PCR), cytokine levels and humoral response. After treatment and during the following 24 months, there was progressive clinical improvement and complete recovery in 50% (7/14) of the treated animals. There was a decrease in the smear positivity of the bone marrow after treatment, and there was also a gradual and constant decrease in positive cultures at the end of the follow-up period. However, the PCR detection of parasite DNA remained positive. In general, all animals presented a significant increase in parasite load 6 months after treatment. The IFN-γ levels in all the groups tended to increase during follow-up period, regardless of the miltefosine dose administered. The IL-4 and IL-10 levels of the animals tended to decrease during follow-up, except after 300 days when only IL-10 increased. The serum antibodies identified antigens that ranged from 116 kDa to less than 29 kDa in the Western blot assay. Furthermore, 300 days after treatment, qualitative and quantitative differences in the antigen profiles were observed. Antigens of 97 and 46 kDa were the most intensely recognized. Higher levels of antigen-specific Leishmania IgG were detected before and 300 days after treatment in all groups. Taking together, the improvement in the clinical symptoms was not followed by parasitological clearance, suggesting that treatment with miltefosine is not recommended, especially in endemic areas like Brazil, where children are the major victims and dogs are involved in the maintenance of the parasite cycle. 相似文献