全文获取类型
收费全文 | 155篇 |
免费 | 8篇 |
国内免费 | 1篇 |
专业分类
林业 | 10篇 |
农学 | 4篇 |
基础科学 | 1篇 |
35篇 | |
综合类 | 20篇 |
农作物 | 7篇 |
水产渔业 | 13篇 |
畜牧兽医 | 65篇 |
园艺 | 2篇 |
植物保护 | 7篇 |
出版年
2022年 | 2篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 2篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 1篇 |
2014年 | 6篇 |
2013年 | 16篇 |
2012年 | 4篇 |
2011年 | 13篇 |
2009年 | 6篇 |
2008年 | 9篇 |
2007年 | 5篇 |
2006年 | 3篇 |
2005年 | 11篇 |
2004年 | 6篇 |
2003年 | 7篇 |
2002年 | 7篇 |
2001年 | 3篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1995年 | 1篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1969年 | 3篇 |
排序方式: 共有164条查询结果,搜索用时 296 毫秒
101.
102.
Kinoshita N Paul M Kashiv Y Collon P Deibel CM DiGiovine B Greene JP Henderson DJ Jiang CL Marley ST Nakanishi T Pardo RC Rehm KE Robertson D Scott R Schmitt C Tang XD Vondrasek R Yokoyama A 《Science (New York, N.Y.)》2012,335(6076):1614-1617
The extinct p-process nuclide (146)Sm serves as an astrophysical and geochemical chronometer through measurements of isotopic anomalies of its α-decay daughter (142)Nd. Based on analyses of (146)Sm/(147)Sm α-activity and atom ratios, we determined the half-life of (146)Sm to be 68 ± 7 (1σ) million years, which is shorter than the currently used value of 103 ± 5 million years. This half-life value implies a higher initial (146)Sm abundance in the early solar system, ((146)Sm/(144)Sm)(0) = 0.0094 ± 0.0005 (2σ), than previously estimated. Terrestrial, lunar, and martian planetary silicate mantle differentiation events dated with (146)Sm-(142)Nd converge to a shorter time span and in general to earlier times, due to the combined effect of the new (146)Sm half-life and ((146)Sm/(144)Sm)(0) values. 相似文献
103.
Antisense transcription in the mammalian transcriptome 总被引:2,自引:0,他引:2
Katayama S Tomaru Y Kasukawa T Waki K Nakanishi M Nakamura M Nishida H Yap CC Suzuki M Kawai J Suzuki H Carninci P Hayashizaki Y Wells C Frith M Ravasi T Pang KC Hallinan J Mattick J Hume DA Lipovich L Batalov S Engström PG Mizuno Y Faghihi MA Sandelin A Chalk AM Mottagui-Tabar S Liang Z Lenhard B Wahlestedt C;RIKEN Genome Exploration Research Group;Genome Science Group 《Science (New York, N.Y.)》2005,309(5740):1564-1566
104.
Biosynthesis of agr and beta-Ecdysones from Cholesterol outside the Prothoracic Gland in Bombyx mori
Labeling experiments have established that cholesterol is converted into alpha-and beta-ecdysones in isolated abdomens of silkworm larvae. Since the isolated abdomens do not contain the prothoracic glands, a doubt is cast on the long-standing principle in insect endocrinology that the prothoracic glands are the source of ecdysone secretion. 相似文献
105.
Production of saturated acyl L-ascorbate by immobilized lipase using a continuous stirred tank reactor 总被引:2,自引:0,他引:2
Watanabe Y Kuwabara K Adachi S Nakanishi K Matsuno R 《Journal of agricultural and food chemistry》2003,51(16):4628-4632
6-O-decanoyl, 6-O-dodecanoyl, or 6-O-tetradecanoyl L-ascorbate was continuously produced at 50 degrees C using a continuous stirred tank reactor (CSTR) with an immobilized lipase, Chirazyme L-2 C2, from Candida antarctica. Acetone was used as the reaction medium. For each saturated acyl L-ascorbate, the productivity of ca. 60 g/L reactor/day was achieved for at least 11 days. The solubility of the saturated acyl L-ascorbate in the soybean oil or water was measured at various temperatures. The solubilities in both the soybean oil and the water were higher for L-ascorbate with a shorter acyl chain. The acyl chain dependence of the solubility in water was stronger than that of the solubility in soybean oil. The temperature dependences of the solubility in both soybean oil and water could be expressed by the van't Hoff equation, and the dissolution enthalpy (DeltaH) values for the soybean oil and water were about 20 and 90 kJ/mol, respectively, irrespective of the acyl chain length. The radical scavenging activities of L-ascorbic acid and the saturated acyl L-ascorbates against 1,1-diphenyl-2-picrylhydrazyl free radical were ca. 95% for all of the compounds, and the introduction of a saturated acyl group to the L-ascorbic acid did not affect the activity. 相似文献
106.
Takeshi Takasaki Kazuma Okada Carlos Castillo Yuki Moriya Toshihiro Saito Yutaka Sawamura Naoko Norioka Shigemi Norioka Tetsu Nakanishi 《Euphytica》2004,135(2):157-167
A new S
9-allele was discovered in 6 Japanese pear cultivars, ‘Shinkou’, ‘Shinsei’, ‘Niitaka’, ‘Amanogawa’, ‘Nangetsu’ and ‘Nansui’.
cDNA encoding S
9-RNase, a stylar product of S
9-allele, was cloned from pistils of ‘Shinkou’ and ‘Shinsei’ by 3' and 5' RACE. The S
9-RNase gene had an open reading frame of 684 nucleotides encoding 228 amino acid residues. S
9-RNase had a hypervariable (HV) region different from S
1- to S
8-RNase and shared higher similarity (95.2%) with apple S
3-RNase than with 8 Japanese pear S-RNases (from 61.0% to 70.7%). Genomic PCR with primers ‘FTQQYQ’ and ‘anti-(I/T) IWPNV’ provided S
1- to S
9-amplicon (product), but could not discriminate the S
2 from the S
9 of ca. 1.3 kb. The S
2 and S
9 were distinguished by digestion with AflII and BstBI, respectively. The digestion with nine S-allele-specific restriction endonucleases, SfcI, AflII, PpuMI, NdeI,AlwNI, HincII, AccII, NruI and BstBI, distinguished S
1 to S
9, establishing that this PCR-RFLP system is useful for S-genotype assignments in Japanese pear harboring S
1- to S
9-allele. ‘Shinkou’, ‘Shinsei’, ‘Nangetsu’ and ‘Nansui’ assigned as S
4
S
9 were determined to be cross incompatible.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
107.
Naruto FURUYA Teruyuki MASUNAGA Abu Ashraf KHAN Kazuhiro IIYAMA Masaru MATSUMOTO Nobuaki MATSUYAMA 《Journal of General Plant Pathology》2000,66(4):316-322
A new bacterial disease of Russell prairie gentian (Eustoma grandiflorum) was found in Fukuoka Prefecture, Japan, in 1997. This disease was characterized by wilting and yellowing of the foliage.
A cross section of the stem of a diseased plant revealed a tan to yellow-brown discoloration of the vascular tissue. A nonfluorescent,
aerobic, Gram-negative bacterium was consistently isolated from infected plants. The bacteriological characteristics of 10
isolates of the bacterium coincided with those of the reference strains of Burkholderia caryophylli that were isolated from carnations. The bacterium, as well as the reference strains, attacked Russell prairie gentian and
carnation after artificial inoculation and reproduced the symptoms similar to those after natural infections. On the basis
of bacteriological characteristics and pathogenicities, the bacterium was identified as B. caryophylli. This is the first report of a disease caused by B. caryophylli on Russell prairie gentian ; therefore, bacterial wilt of Russell prairie gentian is proposed as the name of the disease.
Received 5 April 2000/ Accepted in revised form 11 July 2000 相似文献
108.
The effects of different cell monolayers on in vitro development of early bovine embryos derived from in vitro maturation and fertilization were examined in this study. Early embryos (four to eight cells) were randomly allocated to bovine granulosa cell (GC), oviductal cell (OC), or uterine cell (UC) monolayers in Exp. 1 and to GC, skin cell (SC; from 10-d-old chicken embryos), testicular cell (TC; from 10-d-old mouse), and liver cell (LC; from 10-d-old chicken embryos) monolayers in Exp. 2, and cultured for 6 d at 38.6 degrees C in a humidified atmosphere of 5% CO2 in air. The culture medium was 12.5 mM HEPES TCM 199 supplemented with 1% calf serum and 1 mM sodium pyruvate. In Exp. 1, the percentage of four- to eight-cell embryos that developed to blastocysts on GC, OC, and UC monolayers was 26.9 (28/104), 37.5 (39/104), and 39.2 (40/102), respectively. In Exp. 2, the percentage of four- to eight-cell embryos that developed to blastocysts on GC, SC, TC, and LC monolayers was 53.3 (40/75), 42.9 (33/77), 49.3 (37/75), and 44.3 (35/79), respectively. There were no significant differences in development among groups in either experiment.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
109.
冻拔破坏是季节性冻土地区土中结构常见的破坏形式。为了探明季节性冻土地区土中结构冻拔位移与结构埋深、结构截面形状的关系,利用挤塑保温板加工成长度不等的圆柱体与长方体,埋置于室外冻胀土中,模拟土中结构,开展一个冻融周期的室外自由冻胀试验。观测气温、地温、冻深及土体、圆柱体、长方体的冻拔位移等参数,对比试件的冻胀位移,分析自由冻拔的影响因素。结果表明:当埋深小于临界埋深时,一个冻融周期后2种截面的残余冻胀位移均随埋深增加而快速增大,而当埋深大于临界埋深时,残余冻胀位移随埋深增加无明显变化;埋深相同的长方体与圆柱体的残余冻胀位移相对差均值为10%,考虑到试验过程中其它偶然因素的影响,截面形状对土中结构的自由冻拔无明显影响。 相似文献
110.
Hideaki Shibata Rieko Urakawa Hiroto Toda Yoshiyuki Inagaki Ryunosuke Tateno Keisuke Koba Asami Nakanishi Karibu Fukuzawa Ayaka Yamasaki 《Journal of Forest Research》2011,16(5):374-385
Net nitrogen transformation was investigated under different climate conditions by soil transplantation and in situ incubation
of forest surface soils using the resin-core method. Selected conditions were considered to reflect those of the natural climate
gradient in the Japanese archipelago. Study sites were established in natural forests in northern Hokkaido (Uryu), northern
Kanto (Kusaki), central Kinki (Kamigamo), and southern Kyushu (Takakuma), representing the northernmost to the southernmost
island regions of Japan. Field experiments comparing soils incubated at “native” and “transplanted” sites were conducted from
June 2008 to May 2009. Net production, accumulation, and leaching of soil ammonium (NH4
+) and nitrate (NO3
−) were measured at each of the sites during the growing season (June–October), the dormant season (November–May), and throughout
the year. Net nitrate production was highest in Kusaki soil, especially during the growing season, whereas net ammonium production
was highest in Uryu soil, the coldest site, especially during the dormant season. Net nitrate production increased significantly
in soils transplanted to a warmer climate during the growing season. However, net ammonium production increased in soils transplanted
to colder climates during the dormant season. These findings indicate that, with the exception of the infertile soil samples
from Kamigamo, the range of natural climates in Japan has a significant effect on nitrogen availability in surface soil. In
addition, the original characteristics of the nitrogen cycle of the surface soil from each native site were retained, even
when marked changes in soil temperature (approximately 8°C) occurred after transplantation. 相似文献