Morphometric analysis of Hofbauer cells in normal placenta and chorioamnionitis in humans

Hofbauer cells are macrophages residing in the stroma of placental villi and play a number of roles during normal pregnancy, as well as pathological conditions. A morphometric analysis of Hofbauer cells, in particular to investigate the number of cells, their size and shape in samples of normal human placenta from 1st trimester, term and with chorioamnionitis was performed. Tissue samples were immunostained for CD206 antigen and evaluated using ImageJ software. We detected significant changes in number and morphology of HBCs between normal placenta and placenta with chorioamnionitis samples. In chorioamnionitis, the cells were unevenly distributed within the villi, generally present in higher numbers, larger and more elongated than those in normal 1st trimester and term placenta.     

Occurrence and expression of p53 suppressor gene and c‐Myc oncogene in dog eyelid tumors

Purpose To detect the occurrence and expression of the suppressor gene p53 and of the oncogene c‐Myc in eyelid tumors of dogs using the PCR, RT‐PCR, PCR‐ELISA and RT‐PCR‐ELISA techniques. These genes have not been described in dog eyelid tumors before. Methods Nine samples of eyelid or third eyelid epithelial tumors were obtained from the archives of the Department of Veterinary Pathology. Tumor diagnosis was confirmed by evaluation of hematoxylin‐eosin stained sections, and immunohistochemistry for cytokeratin AE1/AE3 and vimentin V9. A canine mammary tumor was used for positive control. Agarose gel electrophoresis, PCR‐ELISA and RT‐PCR‐ELISA were used to detect p53 and c‐Myc genes. Results The occurrence of p53 was detected in most of the eyelid tumors and third eyelid tumors studied (88.8%, n = 8) and was expressed in 75% of the positive samples, as indicated by ELISA. The c‐Myc gene was found in 77.7% (n = 7) of the samples and was expressed in eight samples. Conclusions Eyelid and third eyelid tumors of dogs express both the p53 and the c‐Myc genes as shown by PCR and RT‐PCR. However, PCR ELISA and RT‐PCR ELISA were more efficient in assessing occurrence and expression of these genes because they identified amplified products that were not detected by agarose gel electrophoresis.     

Sedimentary archive of contamination in the confined channel of the Ohře River,Czech Republic

Purpose

The Oh?e River has received contamination from metal mining and metallurgy (mainly Cu, Pb, Sn, U, Zn) over the previous five centuries. This contamination history has been poorly documented. Contamination has entered the river system in its middle reach, where the channel is incised and bedrock confined, which impedes overbank deposition. Our objective was to locate and describe a sedimentary record in this unfavourable depositional setting.

Materials and methods

Three former channel bars that have coalesced with the riverbank were revealed by examination of historical and current maps and a digital terrain model. Manual coring in the bar and in situ (handheld) X-ray fluorescence (XRF) spectroscopy provided data for developing a contamination chemostratigraphy, which was correlated with the mining history in the region. Detailed topographic examination of the bar and valley edge was important to understanding the evolution of one of the bars. Optically stimulated luminescence (OSL) dating was used to verify the timing of deposition.

Results and discussion

Handheld XRF for in situ analysis of element composition is efficient for studying contaminated sediment bodies with complex stratigraphy, which require extensive coring and stratigraphic correlation. Despite the unfavourable settings, the channel bars trapped sufficient sediment to produce a record that correlates with the history of contamination in the drainage basin. In the bar studied in greatest detail, we observed a surprising amount of contamination passing through the Oh?e River channel (up to 300 mg kg^?1 of Cu, 340 mg kg^?1 of Pb and 630 mg kg^?1 of Sn in fine sand and silt deposits) associated with a pollution climax in the sixteenth and seventeenth centuries. Modern contamination (Hg and U deposited in the nineteenth and twentieth centuries) was entrapped with low efficiency based on comparable concentrations of Hg and U located 90 km downstream.

Conclusions

The efficacy of the use of historical maps and detailed fieldwork was demonstrated by identification of unique depositional meso-environments, which are rare in bedrock-confined fluvial systems. The contamination chemostratigraphy of the bar deposits was correlated with the local mining and pollution history and contributed to an understanding of the bar evolution. The approach used in our study may be applicable to other montane rivers with historic ore mining and processing in their basins.

     

Ecological role of the giant root‐rat (Tachyoryctes macrocephalus) in the Afroalpine ecosystem

Rodents with prevailing subterranean activity usually play an important role in the ecosystems of which they are a part due to the combined effect of herbivory and soil perturbation. This is the case for the giant root‐rat Tachyoryctes macrocephalus endemic to the Afroalpine ecosystem of the Bale Mountains, Ethiopia. We studied the impact of root‐rats on various ecosystem features within a 3.5‐ha study locality dominated by Alchemilla pasture, which represents an optimal habitat for this species, in 2 periods of a year. The root‐rats altered plant species composition, reducing the dominant forb, Alchemilla abyssinica, while enhancing Salvia merjame and a few other species, and reduced vegetation cover, but not the fresh plant biomass. Where burrows were abandoned by root‐rats, other rodents took them over and A. abyssinica increased again. Root‐rat burrowing created small‐scale heterogeneity in soil compactness due to the backfilling of some unused burrow segments. Less compacted soil tended to be rich in nutrients, including carbon, nitrogen and phosphorus, which likely affected the plant growth on sites where the vegetation has been reduced as a result of root‐rat foraging and burrowing.     

Early fetal sexing of Saanen goats by use of transrectal ultrasonography to identify the genital tubercle and external genitalia

OBJECTIVE: To define the optimum period for sexing of Saanen goat fetuses by use of transrectal ultrasonography. ANIMALS: 82 Saanen goats pregnant with 124 fetuses. PROCEDURES: Fetal sexing was performed on the basis of the final location of the genital tubercle or identification of external genitalia. In experiment 1, fetuses (n = 78) were monitored every 48 hours from days 40 to 60 of gestation, whereas for experiment 2, 46 fetuses were examined only once between days 47 and 77 of gestation. RESULTS: For experiment 1, accuracy of fetal sexing was 20 of 20 (100%) for a single fetus, 39 of 42 (92.8%) for twin fetuses, and 10 of 16 (62.5%) for triplet fetuses. Diagnostic accuracy was significantly lower for triplet fetuses than that for single or twin fetuses. Final location of the genital tubercle was detected between 45 and 55 days of gestation (mean +/- SEM, 48.9 +/- 1.8 days). For experiment 2, accuracy of fetal sexing for a single fetus (24/24 [100%]) was significantly higher than the accuracy for twin fetuses (16/22 [72.7%]). Considering all fetuses that were born, accuracy of diagnosis was 69 of 78 (88.4%) for experiment 1 and 40 of 46 (86.9%) for experiment 2. Accuracy did not differ significantly between experiments. CONCLUSIONS AND CLINICAL RELEVANCE: Real-time ultrasonography after day 55 of gestation is a suitable method for determination of sex of Saanen goat fetuses by observation of the genital tubercle or identification of external genitalia.     

Prevalence of crown trauma in free-ranging maned wolves (Chrysocyon brachyurus) in central Brazil

Nineteen free-ranging maned wolves (Chrysocyon brachyurus) were captured in central Brazil from April through November 2003. Oral cavity examination revealed 34 teeth with crown trauma in twelve animals. Canine teeth were most commonly affected (44.2%), followed by premolar (29.4%) and incisor teeth (26.5%). Trauma to maxillary teeth (67.7%) was more frequent compared with mandibular teeth (32.3 %). The majority of dental fractures were located at the cuspid portion of the crown (56.0%). Slab fractures were the most frequent type (37.0%), followed by horizontal (24.0%), oblique (24.0%) and longitudinal fractures (15.0%). Complicated crown fracture (pulp exposure) was diagnosed in 13 (38.2%) of these teeth while pulpitis noted by tooth discoloration was diagnosed in 8 (23.5%) teeth.     

Matcha Tea: Analysis of Nutritional Composition,Phenolics and Antioxidant Activity

Plant Foods for Human Nutrition - Matcha was analysed for its chemical composition, fibre, vitamin C, caffeine and chlorophyll contents. Flavonoids and phenolics, phenolic profiles and antioxidant...     

Geochemical tools for the stratigraphic correlation of floodplain deposits of the Morava River in Strážnické Pomoraví, Czech Republic from the last millennium

The floodplain of the Morava River in Strá?nické Pomoraví, south-east Moravia, Czech Republic contains a very valuable record of regional environmental change, which goes back to several thousand years. Its interpretation has been limited by poor stratigraphic correlation and dating of the sediments. We present a geochemical solution to this challenge. We studied 8 outcrops of floodplain deposits from 4 localities along a 6 km long part of the current meander belt of the Morava River in Strá?nické Pomoraví using geochemical proxy analyses, magnetic susceptibility measurements, ¹⁴C dating of wood remnants, and sediment micromorphology. The proxy methods are based on elemental analysis (EDXRF) and analysis of the cation exchange capacity of clay minerals; granulometric analyses provided the basis for lithological and facies assignment of the sediments. Our geochemical and mineralogical interpretations have further been tested by microstratigraphically studying the optical properties of the fine fraction. Horizons older than about 3 centuries were ¹⁴C dated using wood remnants and the age of deposits from the last century was determined on the base of several proxies reflecting their industrial contamination by heavy metals and magnetic particles. The mean depositional rate over the period from about 1000 to about 1900 AD ranged from 0.2 to 0.6 mm y^− 1, depending on the sedimentary facies. The coeval lithological change in the majority of the studied sections indicated a change of the meander belt structure at between ~ 1200 AD and ~ 1600 AD probably as a consequence of changes of channel structure. The alluvial deposition in the 20th century was strongly affected by the river regulation.     

Cell proliferation and expression of connexins differ in melanotic and amelanotic canine oral melanomas

Melanoma is a malignant neoplasm occurring in several animal species, and is the most frequently found tumor in the oral cavity in dogs. Melanomas are classified into two types: melanotic and amelanotic. Prior research suggests that human amelanotic melanomas are more aggressive than their melanotic counterparts. This study evaluates the behavior of canine melanotic and amelanotic oral cavity melanomas and quantifies cell proliferation and the expression of connexins. Twenty-five melanomas (16 melanotic and 9 amelanotic) were collected from dogs during clinical procedures at the Veterinary Hospital of the School of Veterinary Medicine and Animal Science of the University of São Paulo, Brazil. After diagnosis, dogs were followed until death or euthanasia. Histopathology confirmed the gross melanotic or amelanotic characteristics and tumors were classified according to the WHO. HMB45 or Melan A immunostainings were performed to confirm the diagnosis of amelanotic melanomas. Cell proliferation was quantified both by counting mitotic figures and PCNA positive nuclei. Expressions of connexins 26 and 43 were evaluated by immunohistochemistry, qRT-PCR and Western blot. Dogs bearing amelanotic melanomas presented a shorter lifespan in comparison to those with melanotic melanomas. Cell proliferation was significantly higher in amelanotic melanomas. Expressions of Connexins 26 and 43 were significantly reduced in amelanotic melanomas. The results presented here suggest that oral cavity melanotic and amelanotic melanomas differ regarding their behavior, cell proliferation and connexin expression in dogs, indicating a higher aggressiveness of amelanotic variants.