Sweating sickness: relative curative effect of hyperimmune serum and a precipitated immunoglobulin suspension and immunoblot identification of proposed immunodominant tick salivary gland proteins

Although of low morbidity, sweating sickness is readily induced in calves by infestation with positive Hyalomma truncatum adult ticks. This epitheliotrophic disease has no specific cure except by the administration of hyperimmune serum obtained from animals which have recovered and are subsequently immune to the disease. Treatment with hyperimmune serum, however, has associated problems of donor availability, possible serum contamination and i.v. administration of a relatively large volume. This paper compares the treatment and cure of sweating sickness using unrefined hyperimmune serum and that of an experimental suspension. The latter proved relatively inefficient probably due to a low concentration of effective immunoglobulins. Immunoblot analyses of the sera of affected animals, using tick salivary glands as antigen during the course of the trial revealed 4 tick salivary gland proteins with molecular masses of between 27-33 kDa. These are proposed as being associated with sweating sickness immunodominance.     

Chemical and organoleptic changes in poultry meat resulting from the growth of psychrophylic spoilage bacteria at 1 degree C. I. Introduction and changes in free amino acids

Changes in pH and in the free amino acid content of chicken breast and leg muscle inoculated with micro‐organisms in pure or mixed culture and stored at 1° C have been measured. Control experiments with aseptically prepared antibiotic‐treated samples separated the effects of autolysis from those attributable to the bacteria. A pigmented strain of Pseudomonas and Pseudomonas putrefaciens at counts up to 10⁸ organisms/g produced smaller effects than autolysis, but somewhat larger changes resulted from the growth to 10⁹/g of the pigmented Pseudomonas and, particularly, of a mixed culture of the two bacteria with three others normally present on chill‐stored chicken. The possible effects of these changes on flavour are discussed.     

A survey of the aerobic bacteria in the feces of captive raptors

Feces of 47 captive raptors belonging to the order Falconiformes or Strigiformes were cultured for bacteria. Gram-negative bacteria, which were cultured from the feces of 45 of the 47 raptors, were the most common isolates. A wide variety of species were identified, including a newly described genus (Moellerella wisconsensis), two newly described species (Escherichia fergusonii and Proteus penneri), and a member of a newly described enteric group (CDC Enteric group 41). Additional organisms identified that have not been reported in previous bacteriological surveys of raptors were Salmonella heidelberg, Salmonella braenderup, Morganella morganii, Yersinia ruckeri, Serratia spp., and Kluyvera sp. Escherichia coli, isolated from the feces of 42 of the 47 raptors, was the most frequently recovered. Streptococcus faecalis, the second most common isolate, was cultured from 30 birds. Several differences were observed between fecal bacteria isolated from raptors fed commercially prepared chicken and those isolated from raptors not fed chicken. The most obvious difference was that birds fed chicken had more varied gram-negative bacterial species and in greater numbers per fecal sample. The potential for the isolated bacteria from raptors as pathogens in humans and avian species is discussed.     

Morphology of the pig cornea in normal conditions and after incubation in a perfusion apparatus

The fine structure of the pig cornea in normal conditions and after being used in a perfusion apparatus, for 4 h, is described. Earlier reports on the normal morphology of the pig cornea were partly not confirmed. Thus the number of cell layers in the epithelium was found to be 19-23 (a basal cell layer, 4–5 polyhedral cell layers and 14–17 squamous cell layers) compared to earlier reported 6–9 layers.The mean thickness of normal and perfused corneas were 722 μm and 752 μm respectively. Normal corneas had a hydration level of 77.2 % and after perfusion 78.5 %. The normal morphology and morphological changes due to exposure to perfusion were studied by light and electron microscopy. The differences observed between normal and perfused corneas have to be considered limited, and restricted mainly to the anterior squamous epithelium and the endothelium.Taken together our results indicate that the corneas used in the apparatus still had functional integrity.     

Description and validation of an in situ autoperfusion method to determine nutrient absorption and metabolism in bovine small intestine

Holstein bull calves, 8 to 12 wk of age, were anesthetized with halothane gas. An approximate 20-cm section of small intestine, 60 to 90 cm proximal to the ileocecal junction was clamped to isolate blood circulation to a single set of arcuate vessels and to form an intestinal segment fitted for infusion and drainage. The vein was catheterized to allow total venous collection. Donor blood was transfused via jugular vein to replace venous drainage. This technique was evaluated in four calves by exposing the lumen to eight replications (12 or 20 min incubation, 30-min wash with 39 C saline) of 16 mM L-Met (14C-labeled). Time course appearance of Met in venous blood indicated similar rates and patterns of absorption for individual calves. There were no clinically significant alterations in jugular blood chemistry profiles across replications. Four calves were used to evaluate the effect of three isotonic perfusion media (saline, Krebs-Ringer bicarbonate and M-199 tissue culture media) on Lys and Met absorption. Venous flow rates and absorption of Lys were faster with Krebs buffer than with other media. Perfusate medium did not influence venous flow rates or absorption of Met. Effect of restricting venous flow on absorption of Lys and Met was evaluated in two calves. Flow was alternately controlled (6.5 ml/min) or allowed to flow freely (mean = 12.2 ml/min). Restricting flow decreased steady-state absorption. Light and scanning microscopy indicated maintenance of mucosal tissue integrity throughout 8 h of anesthesia. Results demonstrate validity of the in situ technique to study nutrient absorption in the young bovine.     

Nutritionally altering weight gain patterns of pregnant heifers and young cows changes the time that feed resources are offered without any differences in production

We hypothesized that feed resources could be deferred to a later time in the production cycle without a decrease in fertility or weight of calf produced in heifers and young cows. One-hundred and thirty-one MARC III (four breed composite: (1/4) Hereford, (1/4) Angus, (1/4) Red Poll, and (1/4) Pinzgauer) heifers were divided into three treatments: M-M-M-M (n = 46), L-H-M-M (n = 41), and L-L-L-H (n = 44). The experiment consisted of four feeding periods. Period 1 was 94 to 186 d of gestation, and heifers were fed a moderate (M) or low (L) level of feed. Period 2 was 187 d of gestation to parturition, and heifers were fed moderate, high (H), or low levels of feed. Period 3 was from parturition through 27 d of lactation, and heifers were fed moderate or low levels of feed. Period 4 was from 28 d to approximately 63 d of lactation, and heifers were fed moderate or high levels of feed. Females remained within treatments through their first parity (heifers) and second parity (cows). Feed intake of L-H-M-M and M-M-M-M treatments did not differ from each other either as heifers (P = 0.23) or as second-parity cows (P > 0.59). The L-L-L-H heifers ate less feed than L-H-M-M and M-M-M-M heifers (P < 0.001), and second-parity L-L-L-H cows ate less feed than second-parity L-H-M-M and M-M-M-M cows (P < 0.002). In the first parity, treatments did not differ in the percentage of calves weaned (P = 0.11), weight of calf weaned (P = 0.50), or percentage of cows diagnosed pregnant (P = 0.29) with a second calf. In the second parity, treatments did not differ in the percentage of calves weaned (P = 0.77), weight of calf weaned (P = 0.63), or percentage of cows expressing a corpus luteum at the start of breeding for their third calf (P = 0.21). Our findings suggest that timing nutrient availability to heifers and primiparous cows can be used to change the time that feed resources are used.     

Effects of ketanserin on pulmonary hemodynamics, lung mechanics, and gas exchange in endotoxemic pigs

The effects of ketanserin on pulmonary hemodynamics, lung mechanics, and gas exchange were determined in anesthetized 10- to 14-week-old pigs after they were endotoxemic for 1 or 4.5 hours. Saline solution was given to controls (group 1). Escherichia coli endotoxin (055-B5) was infused IV at a dosage of 5 micrograms/kg for 1 hour (group 2). In group 3, endotoxin was infused at 5 micrograms/kg the first hour plus a continuous infusion of endotoxin at 2 micrograms/kg/hr. Ketanserin, a specific serotonin receptor antagonist, was infused IV (300 micrograms/kg) after pigs were endotoxemic for 1 or 4.5 hours (groups 2 and 3, respectively). At 1 hour of endotoxemia, mean pulmonary artery pressure and pulmonary vascular resistance were increased, and cardiac index was decreased. Ketanserin caused a small attenuation of the increases in mean pulmonary artery pressure and pulmonary vascular resistance, indicating that serotonin may have a small role in the endotoxin response at 1 hour. At 4.5 hours of endotoxemia, mean pulmonary artery pressure, pulmonary vascular resistance, alveolar dead space ventilation, and alveolar-arterial oxygen gradient were increased, and cardiac index and lung dynamic compliance were decreased; ketanserin significantly attenuated the endotoxin-induced changes in cardiac index, mean pulmonary artery pressure, pulmonary vascular resistance, and lung dynamic compliance. Ketanserin also decreased the blood temperature after pigs were endotoxemic for 4.5 hours. However, the endotoxin-induced increases (at 4.5 hours) in alveolar-arterial oxygen gradient and alveolar dead space ventilation were not acutely reversed by ketanserin.(ABSTRACT TRUNCATED AT 250 WORDS)