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81.
Koji MISUMI Yuri HIRAYAMA Sachiko EGAWA Shoko YAMASHITA Hiroyoshi HOSHI Kei IMAI 《The Journal of reproduction and development》2013,59(6):520-524
This study was conducted to clarify the feasibility of newly developed vitrification
techniques for porcine embryos using the micro volume air cooling (MVAC) method
without direct contact with liquid nitrogen (LN2). Expanded blastocysts
were vitrified in a solution containing 6 M ethylene glycol, 0.6 M trehalose and 2%
(wt/vol) polyethylene glycol in 10% HEPES-buffered PZM-5. The blastocysts were
collected from gilts and vitrified using the new device (MVAC) or a Cryotop (CT).
Blastocysts were stored in LN2 for at least 1 month. After warming,
cryoprotective agents were removed using a single step. Survival of the embryos was
assessed by in vitro culture (Experiment 1) and by embryo transfer
to recipients (Experiment 2). In Experiment 1, the embryos vitrified by the MVAC or
CT and fresh embryos without vitrification (Control) were used. The survival rates of
embryos in the MVAC, CT and Control groups were 88.9% (32/36), 91.7% (33/36) and 100%
(34/34), respectively, after 48 h culture, and the hatching rates of embryos after 48
h incubation were 69.4% (25/36), 63.9% (23/36) and 94.1% (32/34), respectively. In
Experiment 2, 64 vitrified embryos were transferred to 5 recipient gilts, and 8
healthy piglets were produced from 3 recipients in the MVAC group. Similarly, 66
vitrified embryos were transferred to 5 recipient gilts, and 9 healthy piglets were
produced from 2 recipients in the CT group. These results indicated that porcine
expanded blastocysts can be cryopreserved using the MVAC method without potential
pathogen contamination from LN2. 相似文献
82.
van Breugel M Hirono M Andreeva A Yanagisawa HA Yamaguchi S Nakazawa Y Morgner N Petrovich M Ebong IO Robinson CV Johnson CM Veprintsev D Zuber B 《Science (New York, N.Y.)》2011,331(6021):1196-1199
Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers. 相似文献
83.
84.
The results presented here support the hypothesis that plants of the tribe Oryzeae respond enantioselectively and homogeneously to optically active 1-alpha-methylbenzyl-3-p-tolylurea (MBTU) in root growth inhibition, in contrast to Echinochloa species. The Oryzeae plants tested in this study belong to different genera (Oryza, Leersia, Chikusichloa and Zizania), to different species (O sativa, O glaberrima, O alta, O coarctata, O latifolia, O minuta, O rufipogon), to various ecospecies of Oryza (japonica, indica, japonica x indica, javanica) and to different levels of evolution [cultivated rice (O sativa and O glaberrima) and ancestral wild rice species]. In spite of their different phylogenic status and diverse sensitivity, the root growth of all members of the genus Oryza was inhibited more by R-MBTU than by S-MBTU. Zizania palustris, Z latifolia, Leersia oryzoides and Chikusichloa aquatica belonging to the tribe Oryzeae exhibited similar chiral recognition to the Oryza plants, suggesting that Oryzeae have a common chiral recognition mechanism in their response to optically active MBTUs. In contrast, Echinochloa plants (E crus-galli (L) Beauv var crus-galli and E colonum (L) Link), belonging into subfamily Panicoideae tribe Paniceae, responded in a different way, where their root growth was more sensitive to S-MBTU than to the antipodal R-MBTU. A reverse chiral response between the tribe Oryzeae and the genus Echinochloa was clearly indicated in this study. This diverse response may be relevant to Gramineae classification. 相似文献
85.
Relationships between immunoglobulin M and immunoglobulin G or A in colostrum of Japanese black multiparous cows 下载免费PDF全文
Saya Taniguchi Mengdong Wang Shuntaro Ikeda Hidetugu Yoshioka Hiroshi Nagase Shoko Kitamura Erina Itoyama Hiroaki Murakami Miki Sugimoto Shinichi Kume 《Animal Science Journal》2016,87(4):536-540
This study was conducted to clarify the relationships among immunoglobulin (Ig)M, IgG, IgA, β‐carotene, vitamin A and α‐tocopherol contents in colostrum of 24 Japanese Black multiparous cows in order to evaluate the role of IgM on colostral IgG and IgA production. Compared with colostral IgG, colostral IgM and IgA were very low but varied widely. There was positive correlation between colostral IgM and IgG, but colostral IgM was not related with colostral IgA. There was no relationship between colostral IgM and age of cows, although colostral IgG was increased with aging. There were positive correlations among colostral β‐carotene, vitamin A and α‐tocopherol and these vitamins were positively correlated with colostral IgM and IgG. These results indicate that fat‐soluble vitamins may affect colostral IgG and IgM in cows and colostral IgG increases with the increase of colostral IgM. 相似文献
86.
Hiroko SHIBATA Shoko TATENO Shuhei KANDA Kazuki TAKAURA Souichi ISHIDA Hisao ITABASHI 《Animal Science Journal》2013,84(2):106-112
The effects of corn dried distillers grains with solubles (DDGS) feeding on rumen fermentation and milk production in cows were evaluated using diets high in neutral detergent fiber (NDF, 45.9–46.6%). The control diet (Control) consisted mainly of hay, corn silage and concentrates. In the experimental diets, the concentrates were replaced with DDGS as 10% dry matter (DM) (10%DDGS) and 20% DM (20%DDGS). Eight cows were used for each 14‐day treatment period. Effect of DDGS feeding on DM intake was not significant. Ruminal volatile fatty acids and ammonia‐N at 5 h after feeding of 20%DDGS were decreased compared to Control, whereas protozoal count at 2 h after feeding of 20%DDGS was higher than that of 10%DDGS. Milk yield of cows fed DDGS diets was greater than that of Control, although percentages of milk protein and solids‐not‐fat were decreased by DDGS diets. The proportions of C10:0, C12:0, C14:0 and C16:0 in the milk fat decreased, and those of C18:0, C18:1, C18:2 and cis‐9, trans‐11 conjugated linoleic acid (CLA) increased markedly with elevated DDGS. Increase in trans‐11 C18:1 was observed in the rumen fluid at 5 h after feeding. These findings suggest that DDGS feeding enhanced milk yield, as well as CLA synthesis under a high dietary NDF condition. 相似文献