离心泵叶轮出口宽度对泵腔内压力脉动分布的影响

在试验和数值模拟相互验证的基础上,开展叶轮出口宽度对离心泵泵腔内压力脉动分布影响的研究．通过试验和数值计算获得离心泵的外特性、泵腔内静压分布、泵腔内压力脉动分布及泵体表面的压力脉动幅值分布,并进行对比分析,结果表明：前泵腔内静压和压力脉动幅值随出口宽度的增大而增大,随半径的减小而增大;后泵腔内静压和压力脉动随出口宽度和半径的变化不十分明显．综合考虑外特性和压力脉动,在比转数 ns ＝97时叶轮出口宽度与叶轮出口直径之比应小于0.06;为了使压力脉动在泵腔内有效地衰减,出口宽度与前腔间隙的比值在1.81附近时最佳．研究结果可用于指导离心泵叶轮的优化设计．     

Molecular cloning,purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.     

Characterization,phylogenetic analyses and pathogenicity of Bipolaris setariae associated with brown stripe disease on sugarcane in Yunnan,China

Brown stripe disease is a severe foliar fungal disease of sugarcane worldwide and is widespread in all sugarcane planting areas in China. Brown stripe is a major disease that seriously affects the output and quality of the sugarcane industry in Yunnan Province, China's second-largest sugar base, while the pathogen of this disease remains not yet fully understood. To address this, we isolated and identified the fungi associated with 68 leaf samples showing typical symptoms of brown stripe from 22 sugarcane varieties in different areas of Yunnan Province. A total of 113 isolates were obtained, which were morphologically similar. Of these, 64 representative isolates were sequenced for the internal transcribed spacer region (ITS), GAPDH and EF-1α loci. All representative isolates grouped with the type strain of Bipolaris setariae in the phylogenetic trees inferred with individual and concatenated sequences of ITS, GAPDH and EF1-α. Pathogenicity test results showed that B. setariae strains were able to induce typical symptoms of brown stripe. The results obtained in this study clarify that only B. setariae is associated with sugarcane brown stripe in Yunnan, China. It is recorded here for the first time as a pathogen causing sugarcane brown stripe in Yunnan, and it is able to infect many major cultivars and new varieties, posing a new threat to the sugar industry in Yunnan Province. In addition, these results provide the scientific basis for the future breeding of disease-resistant varieties and effective prevention and control of sugarcane brown stripe disease.     

丛枝菌根真菌接种对白车轴草耐盐性的影响

丛枝菌根真菌(AMF)可与植物共生形成复杂的菌丝网络,影响植物生长及抗逆能力。目前关于AMF对白车轴草耐盐性的影响尚存争议,本研究采用盆栽试验,研究在盐胁迫条件下(NaCl浓度为150 mmol·L^-1),接种AMF对拉丁诺白车轴草耐盐性的影响。结果表明,在盐胁迫条件下,与对照相比,白车轴草的生长与生理指标均受到抑制。盐胁迫下接种AMF后,白车轴草株高、干重、PSⅡ最大光能转换效率和相对含水量均有增加,丙二醛(MDA)含量以及相对电导率有所降低,渗透调节物质均有提高,其中可溶性糖(SS)和游离脯氨酸(Pro)含量分别提高了32.03%和9.42%。说明盐胁迫抑制白车轴草的生长,接种AMF增强了白车轴草抗逆适应能力,促进白车轴草的生长,增加渗透调节物质含量,提高白车轴草耐盐胁迫的能力。     

Temporal-spatial Filtering in the Preprocessing of Clinical EEG Signals

EEG spot-recording for clinical patients in the bed is always advocated due to easing patients and making them comfortable. But EEG signals recorded in the ward exposed to various noisesand interference is less effective in the EEG analysis. Atemporal-spatial filter is designed for noise removing of clinical EEG signals, the first filtering is temporal filtering using band-pass filter, and the second filtering is spatial filtering with ICA-based spatial filter. Experimental results indicate that various noises and interferences, such as power interference, blinking, eyes movements, muscle moments, ECG artifacts, etc., are removed effectively, individually or simultaneously.     

Spline Boundary Element Method for Calculating Three-Dimensional Electrostatic Fields

Based on [l].this paper presents a new spline-BEM for calculating 3-D electrostatic fields,in which bi-cubic B-splines are used as basis functions. The corner problem in BEM and the extreme conditions for splines are circumvented. The numerical results presented show that the global approximation .convergence and stability of the method are satisfactory.     

The Application of Relevant Homology in the Unified Representation of Electromagnetic Potentials and Gauge Transformation

Electromagnetic potentials and gauge transformation are derived for non-cloSedexterior differential forms with the help of relevant homology.Since de Rham ccahomology is sure-ly a relavant homology. potentials for both closed and non-closed forms and the gauge transforma-tion ean be unified in terms of relavent homology.     

The Unified Representation of Interface Conditionsof Electromagnetic Quantities Using Exterior Differential Forms

A continuity condition of p-forms across a p-dimensional submanifold isderived.The,various interface conditions of electromagnetic quantities are obtained through theapplication of the continuity condition to Minkowski manifold of space-time.