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31.
Seko Takuya Imamura Shintaro Ishihara Kenji Yamashita Yumiko Yamashita Michiaki 《Fisheries Science》2020,86(1):171-179
Fisheries Science - The novel organic selenium compound, selenoneine, is found in the blood of tuna and has metal-binding activity. In this report, selenoneine displays tyrosinase inhibitory... 相似文献
32.
Osamu SAKAI Shoji OGINO Toshihiro TSUKUI Masaya IGASE Takuya MIZUNO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1495
Chimeric antigen receptor (CAR) CAR-T cell therapy targeting CD20 can be a novel adoptive cell therapy for canine patients with B-cell malignancy. After injection of the CAR-T cells in vivo, monitoring circulating CAR-T cells is essential to prove in vivo persistence of CAR-T cells. In this study, we developed a novel monoclonal antibody against canine CD20 CAR, whose single-chain variable fragment was derived from the our previously reported anti-canine CD20 therapeutic antibody. Furthermore, we proved that this monoclonal antibody can detect therapeutic anti-canine CD20 chimeric antibody in the serum from healthy beagle dogs injected with the therapeutic antibody for safety study. This monoclonal antibody is a useful tool for monitoring both canine CD20-CAR-T cells and anti-canine CD20 therapeutic antibody for canine lymphoma. 相似文献
33.
Takuya Nagata Hikari Dobashi Kazuaki Iijima Kazutoshi Ohyama 《Journal of Pesticide Science》2021,46(4):352
Crop field trials were conducted to investigate the residues of sprayed pesticides on the different sizes of tomatoes. Pesticide residue data in tomatoes varied due to different locations of the three crop fields selected and/or physicochemical properties of the three pesticides tested. The pesticide residue levels in the medium- and small-sized tomatoes were 1.5 and 2.4 times higher than the level in large-sized tomatoes under similar spray conditions, whereas amount of pesticides adhered per unit surface area were approximately equal among all three sizes of tomatoes. The results of this study suggested that the differences in pesticide residue levels were due to differences in the degree of specific surface area of each tomato size. Resultant residue data of medium-sized tomatoes demonstrated a proportional relationship between pesticide residue levels and the specific surface area of tomatoes. 相似文献
34.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
35.
Keiichi Murakami Masayuki Hara Takuya Kondo Yohey Hashimoto 《Soil Science and Plant Nutrition》2013,59(5):705-709
Microbial mineralization of urea and uric acid in poultry litter can lead to loss of nitrogen (N) content and its value as a fertilizer. To minimize the loss of N in the composting processes, controlling the water content in litters is a key to reduce the mineralization processes of N compounds. The N content of litter may be influenced by diets, hen age and the type of poultry houses used. The objectives of the present study were i) to determine the relationship between the water content and the decomposition rate of uric acid in poultry litter and ii) to investigate the effect of hen age and crude protein (CP) percentages in diets on the N content of poultry litter. A layer feeding trial was conducted in two poultry farms with windowless and open-floor houses. An incubation study of poultry litter was performed under different levels of water content. Our study found that the diet CP percentage (16.5–18%) and the growth stage of laying hens did not have a significant effect on the amount of total N (52–56?g?kg?1) and uric acid-N (26–31g?kg?1) in fresh litters. At the 7th day of litter incubation study, the concentration of uric acid-N was 22 g kg?1 in litters with a water content of 35%, whereas it further decreased to less than 1.3 g kg?1 in litters with a water content of 55% and higher levels. The decomposition rate of uric acid-N in litter was 0.3–3.1g?kg?1?day?1 in the windowless house and 3.1–7.1g?kg?1?day?1 in the open-floor house. Decomposition of uric acid in litters was positively correlated to the litter moisture content that is controlled to be lower in windowless houses (40–50%) than in open-floor houses (55–80%) during the composting period. Our study suggests that the use of windowless houses for layer chicken production is effective for producing poultry manure with a high N content. 相似文献
36.
Kazuhiro?SakataEmail author Takuya?Kondou Naohiko?Takeshita Akinobu?Nakazono Seir??Kimura 《Fisheries Science》2005,71(2):333-341
Using mark-recapture methods, the movements of the fluvial form of masu salmon (Oncorhynchus masou masou) in a mountain stream on the island of Kyushu, Japan, were studied. Most (78%) of the masu salmon were recaptured in the
pool in which they had been originally caught and tagged. Of those that moved between pools, the proportion of individuals
that moved during the breeding period was not significantly higher than the proportion that moved during the non-breeding
period. However, during the breeding period, a higher proportion of larger salmon moved than did smaller fish. The proportion
of mobile large males during breeding period was higher than that for small males. Also, it was found that a few individuals
showed long-range movement in the autumn. As a long-term movement, 78 individual fish (65%) that were recaptured more than
three times showed high sedentary tendencies. Sixteen individual mobile fish (13%) moved and returned to the original pool.
Fluvial form of masu salmon in Kyushu show a high sedentary nature; however, large mature males seem to actively move in search
of female during breeding period. 相似文献
37.
Shuhei ENJOJI Ryotaro YABE Nobuyuki FUJIWARA Shunya TSUJI Michael P. VITEK Takuya MIZUNO Takayuki NAKAGAWA Tatsuya USUI Takashi OHAMA Koichi SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1451-1456
Canine melanoma is one of the most important diseases in small animal medicine.
Protein phosphatase 2A (PP2A), a well conserved serine/threonine phosphatase, plays a
critical role as a tumor suppressor. SET/I2PP2A is an endogenous inhibitor for PP2A, which
directly binds to PP2A and suppresses its phosphatase activity. Elevated SET protein
levels have been reported to exacerbate human tumor progression. The role of SET in canine
melanoma, however, has not been understood. Here, we investigated the potential
therapeutic role for SET inhibitors in canine melanoma. The expression of SET protein was
observed in 6 canine melanoma cell lines. We used CMeC-1 cells (primary origin) and CMeC-2
cells (metastatic origin) to generate cell lines stably expressing SET-targeting shRNAs.
Knockdown of SET expression in CMeC-2, but not in CMeC-1, leads to decreased cell
proliferation, invasion and colony formation. Phosphorylation level of p70 S6 kinase was
decreased by SET knockdown in CMeC-2, suggesting the involvement of mTOR (mammalian target
of rapamycin)/p70 S6 kinase signaling. The SET inhibitors, OP449 and FTY720, more
effectively killed CMeC-2 than CMeC-1. We observed PP2A activation in CMeC-2 treated with
OP449 and FTY720. These results demonstrated the potential therapeutic application of SET
inhibitors for canine melanoma. 相似文献
38.
We have previously reported that the scallop shell extract possesses free radical scavenging activity. In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radical scavenging, reducing, and ferrous ion-chelating activities. Amino acid composition analysis showed that the 90-kDa protein was rich in Asx (Asp or Asn), Ser and Gly residues. A BLAST search of partial amino acid sequences determined by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry revealed that the 90-kDa protein is a novel protein. The 90-kDa protein is a yellow protein that contains fluorescent substances. To the best of our knowledge, this is the first report of a radical scavenging protein from the scallop shell. 相似文献
39.
Satoshi KAMBAYASHI Masaya IGASE Kosuke KOBAYASHI Ayana KIMURA Takako SHIMOKAWA MIYAMA Kenji BABA Shunsuke NOGUCHI Takuya MIZUNO Masaru OKUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1405-1412
Hypoxic conditions in various cancers are believed to relate with their
malignancy, and hypoxia inducible factor-1α (HIF-1α) has been shown to be a major
regulator of the response to low oxygen. In this study, we examined HIF-1α expression in
canine lymphoma using cell lines and clinical samples and found that these cells expressed
HIF-1α. Moreover, the HIF-1α inhibitors, echinomycin, YC-1 and 2-methoxyestradiol,
suppressed the proliferation of canine lymphoma cell lines. In a xenograft model using
NOD/scid mice, echinomycin treatment resulted in a dose-dependent regression of the tumor.
Our results suggest that HIF-1α contributes to the proliferation and/or survival of canine
lymphoma cells. Therefore, HIF-1α inhibitors may be potential agents to treat canine
lymphoma. 相似文献
40.
Nezu Y Nezu Y Shigihara K Harada Y Yogo T Hara Y Tagawa M 《American journal of veterinary research》2008,69(4):512-518
OBJECTIVE: To determine the effects of intestinal ischemia and reperfusion on the expression of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 mRNAs in the jejunum, liver, and lungs of dogs. ANIMALS: 8 healthy adult Beagles. PROCEDURES: In each dog, the cranial mesenteric artery was occluded for 0 (control group; n=4) or 60 (I-R group; 4) minutes, followed by reperfusion for 480 minutes; serum TNF-alpha and IL-6 activities and expression levels of TNF-alpha and IL-6 mRNAs in jejunal, hepatic, and lung tissues were measured before and at the end of the ischemic period and at intervals during reperfusion. For each variable, values were compared between the control and I-R groups at each time point. RESULTS: Compared with the control group, serum IL-6 activity increased significantly after 180 minutes of reperfusion in the I-R group; also, jejunal TNF-alpha mRNA expression increased significantly after 60 (peak) and 180 minutes of reperfusion. In the I-R group, expressions of IL-6 mRNA in the liver and TNF-alpha and IL-6 mRNAs in the lungs increased significantly at 480 minutes of reperfusion, compared with the control group. Serum TNF-alpha activity, expression of IL-6 mRNA in the jejunum, and expression of TNF-alpha mRNA in the liver in the control and I-R groups did not differ. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that the liver, lungs, and jejunum contributed to the production of TNF-alpha and IL-6 after intestinal ischemia and reperfusion in dogs, suggesting that intestinal ischemia and reperfusion induce a systemic proinflammatory cytokine response in dogs. 相似文献