全文获取类型
收费全文 | 368篇 |
免费 | 14篇 |
专业分类
林业 | 36篇 |
农学 | 11篇 |
56篇 | |
综合类 | 12篇 |
农作物 | 12篇 |
水产渔业 | 52篇 |
畜牧兽医 | 178篇 |
园艺 | 4篇 |
植物保护 | 21篇 |
出版年
2023年 | 1篇 |
2022年 | 10篇 |
2021年 | 22篇 |
2020年 | 9篇 |
2019年 | 9篇 |
2018年 | 12篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 24篇 |
2014年 | 19篇 |
2013年 | 28篇 |
2012年 | 30篇 |
2011年 | 32篇 |
2010年 | 10篇 |
2009年 | 12篇 |
2008年 | 26篇 |
2007年 | 22篇 |
2006年 | 23篇 |
2005年 | 16篇 |
2004年 | 16篇 |
2003年 | 17篇 |
2002年 | 11篇 |
2001年 | 8篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有382条查询结果,搜索用时 15 毫秒
21.
Differentiation of immature mucous cells into parietal, argyrophil, and chief cells in stomach grafts 总被引:2,自引:0,他引:2
Microscopic and submicroscopic studies on regenerating gastric mucosa neonatally grafted in the subcutaneous tissue of littermate mice have revealed that immature mucous cells are totipotent; ultimately they transform into mature mucous, parietal, argyrophil, and chief cells in the gastric glands. 相似文献
22.
Matsuo T Yamaguchi S Mitsui S Emi A Shimoda F Okamura H 《Science (New York, N.Y.)》2003,302(5643):255-259
Cell division in many mammalian tissues is associated with specific times of day, but just how the circadian clock controls this timing has not been clear. Here, we show in the regenerating liver (of mice) that the circadian clock controls the expression of cell cycle-related genes that in turn modulate the expression of active Cyclin B1-Cdc2 kinase, a key regulator of mitosis. Among these genes, expression of wee1 was directly regulated by the molecular components of the circadian clockwork. In contrast, the circadian clockwork oscillated independently of the cell cycle in single cells. Thus, the intracellular circadian clockwork can control the cell-division cycle directly and unidirectionally in proliferating cells. 相似文献
23.
Shimokawa Miyama T Umeki S Baba K Sada K Hiraoka H Endo Y Inokuma H Hisasue M Okuda M Mizuno T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1389-1393
A 4-year-old, intact male Shiba dog was referred to Yamaguchi University Animal Medical Center, Yamaguchi, Japan, for the following complaints: anorexia, lethargy, intermittent fever, gingival bleeding and abdominal purpura. The dog presented with persistent neutropenia. Histopathological examination of a bone marrow sample revealed round to oval structures that resembled Hepatozoon micromerozoites and formed a "wheel-spoke" pattern. Furthermore, mature neutrophils were observed around these structures. PCR and sequencing using bone marrow aspirate confirmed Hepatozoon canis (H. canis) infection. These findings suggest that the neutropenia observed in this case was associated with osteomyelitis due to H. canis infection. This is the first report of neutropenia associated with H. canis infection. H. canis infection can be included in the differential diagnosis in canine cases of neutropenia in areas where the disease is endemic. 相似文献
24.
Suppressive effect of potassium silicate on powdery mildew of strawberry in hydroponics 总被引:1,自引:0,他引:1
Takeshi?KantoEmail author Akihiro?Miyoshi Takuya?Ogawa Kazumasa?Maekawa Masataka?Aino 《Journal of General Plant Pathology》2004,70(4):207-211
From 1996 to 1997, potassium silicate (SiO2) was tested at 0, 25, 50, and 100mgl–1 in hydroponics to control powdery mildew. Other elements were added in the usual amounts, and the strawberries were cultivated hydroponically in a greenhouse for 4 months (from October to January). The powdery mildew spread in the control plot, but little mildew developed in the plot with 25mgl–1 silicate, and none in plots with more than 50mgl–1 silicate. The suppressive effect lasted for about 4 months on fruits and even longer on leaves. On analysis of mineral content in the leaves, only the silicate content differed markedly between the control and treated plants. Nitrogen, phosphate, potassium, and calcium contents did not differ greatly. The maximum silicate content was about 24 times that of the control, and disease severity decreased significantly when the content was more than 1.5% in the leaves. The hardness of the strawberry leaves, measured by a creep meter, was increased by the silicate treatment. 相似文献
25.
Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model 总被引:1,自引:0,他引:1
Lokugamage K Kariwa H Lokugamage N Iwasa M Hagiya T Araki K Tachi A Mizutani T Yoshimatsu K Arikawa J Iwasaki T Takashima I 《The Japanese journal of veterinary research》2004,51(3-4):143-149
The virulence of hantaviruses that are antigenically related but have different genetic characteristics from the prototype of hantavirus, Hantaan (HTN) virus, was examined in newborn mice. The H5 and B78 strains of the Amur (AMR) genotype, the Bao14 strain of the Far East (FE) genotype, and the 76-118 strain of HTN virus were inoculated subcutaneously (1focus-forming unit; FFU) into newborn mice. All of the AMR and FE genotype viruses inoculated mice were died by 16 days post-infection (dpi) and 21 dpi, respectively, while 50% of the HTN virus inoculated mice survived until 30 dpi. The AMR and FE genotype viruses inoculated mice had high viral titers in the lung (1.3x10(6) to 1.3x10(8) FFU/gram [g] tissue) , brain (2.1x10(7) to 1.2x10(9) FFU/g tissue), and kidney(2.5x10(5) to 1.6x10(7) FFU/g tissue), and showed a detectable level of antibodies (titers 1:16-1:32) at 14 dpi. In contrast, the HTN virus infected mice had viruses only in the lungs at low titers (1.1-5.3x10(5) FFU/g tissue). Observations of body-weight changes revealed that the AMR and FE genotype viruses inoculated mice had lower growth rates than the HTN virus inoculated mice. These data suggest that the AMR and FE genotype viruses are more virulent than the HTN virus in newborn mice. 相似文献
26.
Kyoko Sugawara Misako Himeno Takuya Keima Yugo Kitazawa Kensaku Maejima Kenro Oshima Shigetou Namba 《Journal of General Plant Pathology》2012,78(6):389-397
Phytoplasmas are plant pathogenic bacteria that infect more than 700 plant species. Because phytoplasma-resistant cultivars are not available for the vast majority of crops, the most common practice to prevent phytoplasma diseases is to remove infected plants. Therefore, developing a rapid, accurate diagnostic method to detect a phytoplasma infection is important. Here, we developed a phytoplasma detection assay based on loop-mediated isothermal amplification (LAMP) by targeting the groEL gene and 16S rDNA. We designed 19 primer sets for the LAMP assay and evaluated their amplification efficiency, sensitivity, and spectra to select the most suitable primer sets to detect Candidatus Phytoplasma asteris. As a result, DNA was efficiently amplified by one of the primer sets targeting the groEL gene, and LAMP assay sensitivity with this primer set was 10-fold higher than that of the polymerase chain reaction. Moreover, the groEL gene was successfully amplified from several strains of Ca. Phytoplasma asteris by this primer set, indicating that the groEL gene can be used as a LAMP assay target gene for a broad range of phytoplasma strains. Additionally, a simple DNA extraction method that omits the homogenizing and phenol extraction steps was combined with the LAMP assay to develop a simple, rapid, and convenient diagnostic method for detecting phytoplasma. 相似文献
27.
Okawa T Kurio Y Morimoto M Hayashi T Nakagawa T Sasaki N Okuda M Mizuno T 《Research in veterinary science》2012,92(1):80-91
This is the first report describing the expression of canine calreticulin (cCRT) in canine mammary gland tumour (MGT). Using cDNA subtraction method, it is found that mRNAs of CRT, cathepsin A, ovostatin, and lactotransferrin were differentially expressed in mammary adenocarcinoma as compared to hyperplasia, both of which were obtained from the dog. Furthermore, the mRNA expression levels of CRT and cathepsin A were significantly higher in canine MGT samples than in nontumour samples. In contrast, immunohistochemical studies have indicated that the expression of cCRT protein found to be detected in most of mammary gland tissues and was not correlated to the types of canine MGTs. Furthermore, cCRT was molecularly cloned, and the amino acid sequence of cCRT was found to be very similar to those of other species. Further studies are required to elucidate additional roles of cCRT in canine MGT. 相似文献
28.
Takuya Gunji Eiichi Obataya Hidefumi Yamauchi Kenzo Aoyama 《Journal of Wood Science》2012,58(4):369-372
The effects of a carbon fiber-reinforced plastic (CFRP) overlay on the wooden soundboard of a harp were compared to those of conventional veneer reinforcement with respect to the vibrational properties and bending strength. CFRP reinforcement has a minimal effect on the vibrational properties of the soundboard in its width direction, whereas conventional veneer reinforcement significantly reduces the acoustic conversion efficiency of the soundboard. The CFRP-reinforced soundboard also has sufficient bending strength in its longitudinal direction. These results indicate that CFRP is a promising material for the reinforcement of the wooden soundboards of harps to minimize the reduction of the sound amplitude. 相似文献
29.
The vibrational properties of a harp soundboard were investigated with respect to its multi-layered structure. The surfaces of harp soundboards are usually reinforced with veneer; however, this reduces the specific dynamic Young’s modulus (E′/ρ) and significantly increases the internal friction (Q ?1) of soundboards. Since smaller E′/ρ and greater Q ?1 values impart a smaller acoustic conversion efficiency, the attachment of veneer is predicted to reduce the amplitude of the sound produced, as suggested by harp makers. The vibrational properties of veneer-reinforced wood are elucidated using a multi-layered model comprising base wood, a glue layer, veneer and a varnish layer. The results of calculations suggest that a thinner veneer attached with minimal glue would increase the sound amplitude. 相似文献
30.
Ardiansyah Ohsaki Y Shirakawa H Koseki T Komai M 《Journal of agricultural and food chemistry》2008,56(8):2825-2830
We studied the effects of a single oral administration of ferulic acid (FA) on the blood pressure (BP) and lipid profile in stroke-prone spontaneously hypertensive rats (SHRSP). Male 12-week-old SHRSP were administered FA (9.5 mg/kg of body weight) and distilled water as the control (C) (1 mL) via a gastric tube. The hypotensive effect of FA was observed at the lowest value after 2 h administration. A decrease in the angiotensin-1-converting enzyme (ACE) activity in the plasma corresponded well with the reduction of BP. Plasma total cholesterol and triglyceride levels were lower after 2 h administration. The mRNA expression of genes involved in lipid and drug metabolism was downregulated in the FA group. These results suggest that oral administration of FA appears beneficial in improving hypertension and hyperlipidemia. 相似文献