Strategy for implementing silvicultural practices in Japanese plantation forests to meet a carbon sequestration goal

The strategy for implementing silvicultural practices in Japanese plantation forests was examined to achieve a carbon uptake of 13.0 Mt-C year^–1, which was capped by the Marrakesh Accords, during the first commitment period, 2008–2012. The plantation forests that implemented silvicultural practices in the period 1990–2012 (FM plantation forests) were identified in compliance with the hypothesized identification rules, and carbon uptake in the forests was estimated using a simple model composed of simulation and optimization components on the assumption that whole plantation forest is classified into only two groups. Furthermore, parametric analysis was conducted to investigate the relationships among three factors: (1) the average annual harvesting volume (including thinning) in plantation forests during the first commitment period, (2) the total area of FM plantation forests in 2012, and (3) the average annual amount of carbon to be sequestered in FM plantation forests during the first commitment period. The results imply that young stands have to be prioritized in implementing silvicultural practices under any harvesting plan and carbon sequestration goal, and that FM plantation forests sequestered 8.0–10.5 (Mt-C year^–1) carbon in inverse proportion to the harvesting volume of 21.0–14.0 million m³ year^–1 (log volume), assuming that most of the plantation forests were incorporated into them.     

Occurrence of boscalid resistance in cucumber powdery mildew in Japan and molecular characterization of the iron–sulfur protein of succinate dehydrogenase of the causal fungus

A total of 74 mass isolates of cucumber powdery mildew fungus (Podosphaera xanthii) were collected from commercial greenhouses with a history of boscalid use in Ibaraki Prefecture, Japan, and tested in a leaf disk assay for their sensitivity to boscalid. The mildew development of 40 of 74 isolates and five sensitive reference isolates on the disks was completely suppressed at 5 μg boscalid/ml. The minimum inhibitory concentrations (MIC) for the remaining 34 isolates were 50 μg/ml or higher, and 21 of these isolates also grew well at 500 μg/ml. Six single-spore isolates were resistant to boscalid with MIC values higher than 500 μg/ml; four of these were moderately resistant (MR), and two were very highly resistant (VHR) isolates. The growth of MR isolates was almost completely suppressed at 500 μg/ml, whereas two VHR isolates grew vigorously at 500 μg/ml. In foliar inoculation tests of potted cucumber plants, the efficacy of boscalid (500 μg/ml) against both MR and VHR isolates was very low. Partial DNA fragment of the iron–sulphur protein subunit (SdhB) gene of succinate dehydrogenase was PCR-amplified from five sensitive and five resistant isolates and directly sequenced, revealing that VHR isolates possess a substitution from a highly conserved histidine (CAT) to tyrosine (TAT) in a third cysteine-rich center of a putative SdhB, whereas MR isolates so far have not been found to have such substitution in SdhB.