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921.
Takeshi Yamamoto Takanobu Goto Yuta Kine Yuta Endo Yuichi Kitaoka Tsuyoshi Sugita Hirofumi Furuita Yasuro Iwashita & Nobuhiro Suzuki 《Aquaculture Research》2008,39(9):986-994
A feeding experiment was conducted to examine the effect of a supplemental ethanol extract from a defatted soybean meal on the biliary bile status and intestinal conditions of rainbow trout Oncorhynchus mykiss . A semi-purified control diet based on casein (Cont) and three diets supplemented with the extract (ES), bovine bile salts (BS) and their combination (ESBS) were fed to trout (10 g) for 6 weeks. The growth, feed efficiency ratio, fat digestibility and gallbladder- and hepato-somatic indices of fish fed diet ES decreased. Compared with this group, these parameters were improved in fish fed diet ESBS. The total biliary bile salt content was the lowest in fish fed diet ES and this group had a high proportion of chenodeoxycholyltaurine. The inclusion of the bile salts to diet ES (diet ESBS) increased the total biliary bile salt content. A similar trend was observed in the bile salt concentration of intestinal digesta. Although morphological changes occurred in epithelial cells of the distal intestine of fish fed diet ES, the histological features of fish fed diet ESBS were similar to those of fish fed diet Cont. These results indicate that alcohol-soluble substances of soybean meal cause physiological changes in the biliary bile and intestine of rainbow trout. 相似文献
922.
In separate feeding trials, channel catfish fingerlings were fed 0, 1000, 4000, 8000, 20,000, 50,000, and 0, 500, 1000, 2000, 4000, 8000 I.U./kg diet of cholecalciferol and ergocalciferol for 28 weeks. The supplemental requirement for maximal growth was between 1000 and 4000 I.U. of supplemental cholecalciferol/kg diet. At supplemental levels of 1000 I.U./kg diet or less, the growth responses to ergocalciferol were equal to those obtained from equivalent levels of cholecalciferol. However, at higher supplemental levels of ergocalciferol, the weight gains were significantly less than those obtained from 2000 to 20,000 I.U./kg of cholecalciferol. Weight gains were significantly reduced when 50,000 I.U./kg of cholecalciferol were fed. Vertebrae bone ash values were not affected by dietary vitamin D levels. Reduced weight gains and feed efficiency were the only signs of vitamin D deficiency or hypervitaminosis D. 相似文献
923.
924.
Rui KANO Tomoko TAKAHASHI Takeshi HAYAKAWA Yoshiki YAMAYA Atsuhiko HASEGAWA Hiroshi KAMATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1183-1185
Feline upper respiratory tract infection due to Aspergillus spp. is
considered an emerging disease, with the number of reported cases continuing to rise. In
this study, we report the first case of feline sinonasal aspergillosis caused by
Aspergillus fischeri in Japan. The patient presented after 2 months of
progressive facial deformity around the nose and nasal discharge. The isolate from this
case was susceptible to itraconazole (ITZ), voriconazole and micafungin, but was resistant
to amphotericine B. However, the infected cat died approximately 1 month after referral,
despite treatment for 12 days ITZ administered orally at 10 mg/kg. 相似文献
925.
Konosuke OTOMARU Takuro FUJIKAWA Yasuo SAITO Takaaki ANDO Takeshi OBI Naoki MIURA Chikara KUBOTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1191-1193
A 10-month-old Japanese black heifer was diagnosed as having an intra-abdominal
cyst using computed tomography (CT). Through a posterior ventral midline incision, the
cyst was removed, and the heifer completely recovered after the surgery. CT scans enabled
detection of the intra-abdominal cyst and measurements of the diameter of the cyst before
the surgery. 相似文献
926.
Takeshi Watanabe Atsuhiko Kato Hiromichi Terashima Koichi Matsubara Yu Jau Chen Kenji Adachi Hideaki Mizuno Masami Suzuki 《Journal of toxicologic pathology》2015,28(1):43-49
Recently, large-scale gene expression profiling is often performed using RNA extracted from unfixed frozen or formalin-fixed paraffin embedded (FFPE) samples. However, both types of samples have drawbacks in terms of the morphological preservation and RNA quality. In the present study, we investigated 30 human prostate tissues using the PFA-AMeX method (fixation using paraformaldehyde (PFA) followed by embedding in paraffin by AMeX) with a DNA microarray combined with laser-capture microdissection. Morphologically, in contrast to the case of atypical adenomatous hyperplasia, loss of basal cells in prostate adenocarcinomas was as obvious in PFA-AMeX samples as in FFPE samples. As for quality, the loss of rRNA peaks 18S and 28S on the capillary electropherograms from both FFPE and PFA-AMeX samples showed that the RNA was degraded equally during processing. However, qRT-PCR with 3’ and 5’ primer sets designed against human beta-actin revealed that, although RNA degradation occurred in both methods, it occurred more mildly in the PFA-AMeX samples. In conclusion, the PFA-AMeX method is good with respect to morphology and RNA quality, which makes it a promising tool for DNA microarrays combined with laser-capture microdissection, and if the appropriate RNA quality criteria are used, the capture of credible GeneChip data is well over 80% efficient, at least in human prostate specimens. 相似文献
927.
Mohamed Elshabrawy GHANEM Erisa TEZUKA Bhuminand DEVKOTA Yoshiaki IZAIKE Takeshi OSAWA 《The Journal of reproduction and development》2015,61(1):54-60
We investigated the relationship between the persistence of uterine bacterial infections with cytologically determined endometritis and ovarian function in 65 postpartum Holstein cows. Vaginal mucus discharges were collected, and endometrial smear samples (n = 130) were collected for cytological and bacteriological examinations from the cows at weeks 5 and 7 postpartum (pp). Blood samples were collected at weeks 3, 5 and 7 pp to determine plasma progesterone concentrations to monitor ovarian activity. According to the bacteriological examination, cows were classified into four groups. The first group (n = 32; 49%) comprised cows negative for bacteria at weeks 5 and 7 pp. The second group (n = 11; 17%) comprised cows with bacterial infections at week 5 pp but that were clear of infection at week 7 pp. The third group (n = 12; 19%) comprised cows without bacteria at week 5 pp but that acquired an infection by week 7 pp. The fourth group (n = 10; 15%) comprised cows with
bacterial infections at weeks 5 and 7 pp (persistence of infection). A positive correlation (P < 0.001) was noted between the severity of cytologically determined endometritis, purulent vaginal discharge and the persistence of infection. Cows with persistent infections had a significantly (P < 0.01) prolonged luteal phase compared with cows without infection. In conclusion, the prevalence of cytologically determined endometritis and prolonged luteal phase were significantly increased in cows with persistent infections. 相似文献
928.
929.
Takashi Yamanoshita Tanit Nuyim Masaya Masumori Takeshi Tange Katsumi Kojima Hisayoshi Yagi Satohiko Sasaki 《Journal of Forest Research》2001,6(3):217-219
We investigated the growth response ofMelaleuca cajuputi Powell to flooding at 3 sites in a tropical swamp in southern Thailand. The relative growth rate (RGR) in height tended to be higher where the water level was higher. The maximumRGR in height was achieved during the flood period at the 2 sites where water levels were higher than at the other site. The
height growth ofM. cajuputi was not reduced by flooding, but enhanced. No decline in growth due to post-anoxic injury was observed after flooding.Melaleuca cajuputi may have mechanisms to tolerate rhizospheric oxygen deficiency and to avoid post-anoxic injury.
This study was supported by a Grant-in-Aid for Basic Research (No. 09NP0901) from the Ministry of Education, Science, Sports,
and Culture of Japan. 相似文献
930.
Takeshi?Nagasaki Norihisa?Osada Mika?Sato Seiichi?Yasuda Koetsu?Takahashi Takanori?ImaiEmail author 《Journal of Wood Science》2004,50(1):82-86
The reactivity of previously prepared antiserum against agatharesinol, a norlignan, was determined by competitive inhibition–ELISA (enzyme-linked immunosorbent assay) using several natural compounds that are representative of plant extractives as competitive inhibitors. The antiserum strongly reacted with some norlignans (i.e., agatharesinol, hinokiresinol, and metasequirin C), each of which has a common chemical-structural unit. This result suggests that the antiserum recognizes the specific chemical-structural unit composing those norlignans. Moreover, differences in the reactivities of the antiserum with agatharesinol derivatives and synthetic chalcones propose the trans-3-p-hydroxyphenyl-1-phenylpropene structural unit as the major antigenic determinant for the antiserum.Part of this paper was presented at the 45th Lignin Symposium, Matsuyama, October 2000 and the 51th Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001 相似文献