Addition of <Emphasis Type="Italic">Pestalotiopsis </Emphasis>spp. to leaf spot pathogens of Japanese persimmon

 In June 1996, a leaf spot disease widely occurred in Japanese persimmon (Diospyros kaki) orchards in Tottori Prefecture, Japan. The main diagnostic symptom was ring spot on the leaves and calyxes of young fruits; in severe cases, lesions developed on more than half of the area of the leaf, resulting in early defoliation. Based on morphological and pathological studies of the isolated fungi, it was shown that Pestalotiopsis longiseta, P. glandicola, P. acaciae, and P. crassiuscula were responsible for the diseases. These fungi, except P. longiseta, were found to be new pathogens of the disease. Received: May 20, 2002 / Accepted: July 25, 2002     

Circular Leaf Spot of Sweet Basil Caused by <Emphasis Type="Italic">Cercospora guatemalensis </Emphasis>New to Japan

A leaf spot disease on sweet basil (Ocimum basilicum L.) was observed in Chiba Prefecture in 1997. The lesions were amphigenous, circular to irregular, hygrophanous, dark brown. Stromata were amphigenous, composed of a few brown cells. Conidia with a thickened truncate base, filiform to cylindrical, hyaline, were produced from conidiophores with thickened conidial scars. These morphological characteristics agreed with those of Cercospora guatemalensis Mueller et Chupp. This species was new to Japan. The symptoms appeared 10 days after inoculation and the inoculated fungus was re-isolated. Artificial sporulation and detection from commercial seeds were not successful. Received 7 May 2001/ Accepted in revised form 7 August 2001     

Shoot Blight and Leaf Spot of Blueberry Anthracnose Caused by <Emphasis Type="Italic">Colletotrichum acutatum</Emphasis>

Shoot blight and leaf spots were found on highbush blueberry trees in Tsukuba, Ibaraki, in 1999. The causal fungus was identified morphologically as Colletotrichum acutatum Simmonds ex Simmonds. This is the first report of blueberry anthracnose caused by C. acutatum in Japan. Received 16 November 2001/ Accepted in revised form 22 March 2002     

Brown Leaf Spot on Lantana spp. Caused by Pseudocercospora guianensis

Brown leaf spot of Lantana camara L. and L. montevidensis Briq. caused by Pseudocercospora guianensis (Stevens et Solheim) Deighton was found in Shizuoka, Chiba, Kagoshima and Okinawa Prefectures. Pathogenicity of isolates from the leaf spots was examined, and a taxonomic study as well as identification of the causal fungus was carried out. Similar leaf spots appeared on 7 to 25 days after inoculation with the isolates on Lantana spp., and the same fungus was re-isolated from the inoculated leaves. Two hitherto known Pseudocercospora species on Lantana, P. guianensis and P. formosana, were considered to be variations within one species, and we identified the causal fungus as P. guianensis due to priority. Received 25 September 2000/ Accepted in revised form 20 May 2001     

Lipophilic,hydrolytic and photolytic properties and fungicidal activity against Botrytis cinerea of 1-(4-substituted phenoxymethyl)-2, 2-dimethylpropyl imidazole-1-carboxylates and -thiocarboxylates

The sterol 14α-demethylation inhibitors 1-(4-substituted phenoxymethyl)-2, 2-dimethylpropyl imidazole-1-carboxylates and their corresponding thiocarboxylates were optimized for maximum in-vitro activity against Botrytis cinerea Pers. ex Fr. in terms of the lipophilic parameter π. The activity of both carboxylates and thiocarboxylates was strongly related with π and predicted to be maximum at π 1.38 and 0.72 respectively. However, the preventive efficacy of the carboxylates against B. cinerea on Solanum melongena L. grown in a greenhouse did not correlate with the parameter. Despite the unfavourable lower lipophilicity, compounds with p-alkoxy substituents were superior to those with any other substituent. Moreover, the p-methoxy-substituted thiocarboxylate 46 was much less effective in greenhouse tests than the corresponding carboxylate 15, despite their equivalent in-vitro activity. To clarify these discrepancies, hydrolytic and photolytic stabilities of several representative compounds including 15 and 46 were investigated. The carboxylates examined were much more labile to hydrolysis than the thiocarboxylate 46; however, the four thiocarboxylates studied were less stable to light than the carboxylates. Consequently, the above discrepancies were attributable mainly to the superior stability of the carboxylates to photolysis as compared to the thiocarboxylates.     

Possibility of quantitative assessment of the contribution of paddy irrigation and caldera lakes to river water in Bali Island using water isotopic physics

Paddy and Water Environment - This study aims to investigate a possibility of quantitative assessment of the relationship between return flow from paddy fields and river regime by using hydrogen-...     

Assessment of changes in water cycles on food production and alternative policy scenarios

This special issue deals with water and food as it applies to water resources and rice production in the Mekong River Basin. The range of papers reflects not only the broad interest but also the complexity of the topic. These reports are mainly based on the research carried out by the project, “Assessment of the Impact of Global-Scale Change in Water Cycles on Food Production and Alternative Policy Scenarios”. Hence the special issue gives a brief overview of the structure, goals, outcomes, and future direction of the above project. What we do in this paper are: (1) giving an overview of the project’s structure and goals, (2) stating the five common outcomes and several more specific results, and (3) looking forward to the future direction of the project. The aim of our research is to draw up scenarios for optimum water-resource distribution and to develop social guidelines, measures, and policies to help solving food and environmental problems by developing a world water–food model that emphasizes rice production in Monsoon Asia. We have integrated all of the data gathered and the research results into a food supply and demand model combined with a water-cycle analysis. We included operational factors, such as water-cycle change, water demand, water supply, and water distribution in the model. Emphasis is placed on the use of efficient resource-management technologies for proper use of water resources in agricultural and other sectors. The water–food model has been developed as a tool for evaluating technical decisions derived under various policy scenarios.     

Stand parameter estimation of artificial evergreen conifer forests using airborne images: An evaluation of seasonal difference on accuracy and best wavelength

The relationship between the stand parameters (top layer height (H1) and volume/ha (Vha)) and digital number (DN) were evaluated for evergreen conifer stands using three airborne images with 4-m spatial resolution, which were taken in June 1995, September 1993, and October 1994 using the Compact Airborne Spectrographic Imager (CASI). Estimation accuracy of the stand parameters, their seasonal changes, and suitable wavelength were analyzed using correlation coefficients and a regression analysis. The minimum DN of stands, which showed the darkness of a canopy shadow, had a higher correlation with H1 than the average and maximum DN while the average DN had a higher correlation with Vha. The green channels gave the highest correlation coefficients with H1 and Vha, which exceeded — 0.9 for the September and October images. However, the red channels had a consistently high correlation with the stand parameters for the three images. The near infrared channels gave poor correlations with H1 and Vha for the June image. Spectral variations among trees may affect the relationship between DN and the stand parameters in the leaf maturation period in June. Consequently, the late growing season was better at giving consistent results for the stand parameter studies. There was a linear relationship between the measured and the estimated stand parameters for the validation plots especially for the H1 case of September with sufficient accuracy. Nadir viewing images, which had high spatial resolution and a wide dynamic range such as the CASI images, were necessary to estimate the stand parameters accurately. This study was also partly supported by the research project, ‘A Study of Accurate Biomass Estimation using Airborne Imagery,’ of the Science and Technology Agency of Japan. Comments from the editors helped improve the contents of the paper.     

Characterization of <Emphasis Type="Italic">Trichoderma</Emphasis> species isolated in Ecuador and their antagonistic activities against phytopathogenic fungi from Ecuador and Japan

Native Trichoderma spp. were isolated from agricultural fields in several regions of Ecuador. These isolates were characterized via morphological observation as well as molecular phylogenetic analysis based on DNA sequences of the rDNA internal transcribed spacer region, elongation factor-1α gene and RNA polymerase subunit II gene. Fifteen native Trichoderma spp. were identified as T. harzianum, T. asperellum, T. virens and T. reesei. Some of these strains showed strong antagonistic activities against several important pathogens in Ecuador, such as Fusarium oxysporum f. sp. cubense (Panama disease) and Mycosphaerella fijiensis (black Sigatoka) on banana, as well as Moniliophthora roreri (frosty pod rot) and Moniliophthora perniciosa (witches’ broom disease) on cacao. The isolates also showed inhibitory effects on in vitro colony growth tests against Japanese isolates of Fusarium oxysporum f. sp. lycopersici, Alternaria alternata and Rosellinia necatrix. The native Trichoderma strains characterized here are potential biocontrol agents against important pathogens of banana and cacao in Ecuador.