全文获取类型
收费全文 | 195篇 |
免费 | 4篇 |
国内免费 | 1篇 |
专业分类
农学 | 6篇 |
34篇 | |
综合类 | 11篇 |
农作物 | 6篇 |
水产渔业 | 13篇 |
畜牧兽医 | 110篇 |
植物保护 | 20篇 |
出版年
2022年 | 9篇 |
2021年 | 14篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 11篇 |
2014年 | 13篇 |
2013年 | 13篇 |
2012年 | 8篇 |
2011年 | 7篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 18篇 |
2007年 | 13篇 |
2006年 | 11篇 |
2005年 | 10篇 |
2004年 | 9篇 |
2003年 | 12篇 |
2002年 | 15篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1997年 | 1篇 |
1993年 | 2篇 |
1958年 | 3篇 |
1957年 | 2篇 |
1951年 | 1篇 |
排序方式: 共有200条查询结果,搜索用时 15 毫秒
121.
Yuichi Ozaki Hidehiro Koga Takako Takahashi Shinji Adachi Kohei Yamauchi 《Fisheries Science》2008,74(2):362-371
ABSTRACT: Changes in lipid content and fatty acid composition of muscle, liver and ovary of captive-reared and wild silver Japanese eel Anguilla japonica were examined during artificial maturation induced by salmon pituitary homogenate (SPH) injections. Although the relative levels of n-3 and n-6 highly unsaturated fatty acids (HUFA) in liver and ovary were higher than in muscle in both captive and wild silver eels before SPH injection, these tended to decrease with maturation. The relative levels of n-6 HUFA in muscle, liver, ovary and eggs of wild silver eels were remarkably higher than those in captive eels. Therefore, we attempted to alter the ratio of n-6 HUFA in eggs by feeding eels a diet supplemented with linoleic acid-rich plant oil. Although the percentage of n-6 polyunsaturated fatty acids to total fatty acids in eggs of eels fed the supplemented diet was similar to wild silver eels, the percentage of n-6 HUFA remained remarkably lower than in wild silver eels. Hence, it appears that the supplemented diet affected the fatty acid composition of eggs, but did not result in much conversion of linoleic acid to its higher homologs in eels. 相似文献
122.
Misaki NISHI Shouta SUGIO Tetsushi HIRANO Daisuke KATO Hiroaki WAKE Asuka SHODA Midori MURATA Yoshinori IKENAKA Yoshiaki TABUCHI Youhei MANTANI Toshifumi YOKOYAMA Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):585
Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effects of the NN clothianidin (CLO) by in vivo Ca2+ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca2+ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis. 相似文献
123.
Yixian WEI Ai HORI I-Ying CHEN Haruka TAMOGI Taku HIROKAWA Keiko KATO Takaharu ITAMI Tadashi SANO Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):792
Recently, a mucosal atomization device (MAD) has been applied in veterinary medicine. In the present study, the maximum volume of nasal atomization without aspiration using MAD was examined in eight healthy female Japanese White (JW) rabbits. Each rabbit had their head and neck examined by computed tomography before and after nasal atomization with four different doses (0.15, 0.3, 0.45, and 0.6 ml per nostril) of diluted contrast medium (1:2 mixture of iohexol and saline). This was done under general anesthesia by an intramuscular administration of alfaxalone 2.5 mg/kg, medetomidine 40 μg/kg, and butorphanol 0.4 mg/kg, with a 7-day washout period between each treatment. The diluted contrast medium was distributed in the nasal cavity, external nares, and/or oral cavity in all rabbits receiving each treatment. The intranasal distribution volumes of the contrast medium were 287 (250–333) mm3 [median (interquartile range)] for 0.15 ml, 433 (243–555) mm3 for 0.3 ml, 552 (356–797) mm3 for 0.45 ml, and 529 (356–722) mm3 for 0.6 ml of treatment. The intranasal distribution volume for 0.15 ml treatment tended to be lower than that for 0.6 ml treatment (P=0.083). The contrast medium was deposited in the trachea in one rabbit (12.5%) and four rabbits (50%) receiving treatments of 0.45 and 0.6 ml per nostril, respectively. The maximum volume of nasal atomization without aspiration into the trachea was 0.3 ml per nostril for the JW rabbits. 相似文献
124.
125.
Amla (Emblica officinalis Gaertn.) attenuates age-related renal dysfunction by oxidative stress 总被引:1,自引:0,他引:1
Yokozawa T Kim HY Kim HJ Tanaka T Sugino H Okubo T Chu DC Juneja LR 《Journal of agricultural and food chemistry》2007,55(19):7744-7752
To investigate the effects of amla on renal dysfunction involved in oxidative stress during the aging process, we employed young (2 months old) and aged (13 months old) male rats and administered SunAmla (Taiyo Kagaku Co., Ltd., Japan) or an ethyl acetate (EtOAc) extract of amla, a polyphenol-rich fraction, at a dose of 40 or 10 mg/kg body weight/day for 100 days. The administration of SunAmla or EtOAc extract of amla reduced the elevated levels of serum creatinine and urea nitrogen in the aged rats. In addition, the tail arterial blood pressure was markedly elevated in aged control rats as compared with young rats, while the systolic blood pressure was significantly decreased by the administration of SunAmla or EtOAc extract of amla. Furthermore, the oral administration of SunAmla or EtOAc extract of amla significantly reduced thiobarbituric acid-reactive substance levels of serum, renal homogenate, and mitochondria in aged rats, suggesting that amla would ameliorate oxidative stress under aging. The increases of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expression in the aorta of aging rats were also significantly suppressed by SunAmla extract or EtOAc extract of amla, respectively. Moreover, the elevated expression level of bax, a proapoptotic protein, was significantly decreased after oral administration of SunAmla or EtOAc extract of amla. However, the level of bcl-2, an antiapoptotic protein, did not show any difference among the groups. The expressions of renal nuclear factor-kappaB (NF-kappaB), inhibitory kappaB in cytoplasm, iNOS, and COX-2 protein levels were also increased with aging. However, SunAmla or EtOAc extract of amla reduced the iNOS and COX-2 expression levels by inhibiting NF-kappaB activation in the aged rats. These results indicate that amla would be a very useful antioxidant for the prevention of age-related renal disease. 相似文献
126.
Tsuji M Zamoto A Kawabuchi T Kataoka T Nakajima R Asakawa M Ishihara C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(7):643-646
Six Eurasian red squirrels (Sciurus vulgaris orientis), victims of road traffic found during 2002 and 2004 near the Noppro Forest Park in Ebetsu, Hokkaido, Japan, were examined for the presence of Babesia parasites. Three of the six squirrels exhibited positive signals by nested PCRs targeting both the 18S rRNA and beta-tubulin genes. Three squirrels proved to be infected with a B. microti-like parasite as evidenced by sequencing the amplified DNAs and by the morphology of the intraerythrocytic parasites. Genotypically, however, the parasite appeared to be of a new type, as it was clearly distinguishable from any of the known types that have previously been reported in various wild animals. This is the first report showing molecular evidence for the presence of B. microti-like parasites in Sciuridae. 相似文献
127.
Masaya IGASE Kenji BABA Takako SHIMOKAWA MIYAMA Shunsuke NOGUCHI Takuya MIZUNO Masaru OKUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1507-1510
A 6-year-old spayed female Jack Russell Terrier presented with a 1-month
history of lethargy, anorexia, vomiting and weight loss. The dog was fed beef and chicken
jerky treats daily in addition to a commercial diet. Laboratory tests revealed azotemia,
hypokalemia, hyperchloremia, metabolic acidosis and glucosuria with normoglycemia. Urine
amino acid analysis showed significant amino acid loss into the urine. Thus, Fanconi
syndrome was diagnosed, and based on the case history and extensive diagnostic testing,
excessive consumption of jerky treats was strongly suspected as the cause. Glucosuria
resolved 7 days after the withdrawal of jerky treats and fluid therapy. Aminoaciduria was
substantially, but not completely, improved 3 months after diagnosis. Mild azotemia
remained, suggesting chronic renal disease. To the best of our knowledge, this is the
first reported case of Fanconi syndrome following the consumption of jerky treats in
Japan. 相似文献
128.
Kazuko OGATA Aiko SASAKI Yuka KATO Arisa TAKEDA Mikio WAKABAYASHI Borjigin SARENTONGLAGA Mio YAMAGUCHI Asuka HARA Rika FUKUMORI Yoshikazu NAGAO 《The Journal of reproduction and development》2015,61(2):116-122
The present study was conducted to evaluate whether supplementation of semen extender with glutathione (GSH) can maintain the quality of frozen-thawed canine spermatozoa. Eighteen ejaculates were obtained from 5 dogs and placed in extender (20% egg yolk, Tris, citric acid, lactose, raffinose, antibiotics and 6.5% glycerol) containing 0 (control), 2.5, 5, 7.5 or 10 mM GSH. The samples were cooled to 4 C and then frozen in liquid nitrogen vapor. Motility parameters of the sperm were evaluated at 0, 1, 2, 3, 4, 12 and 24 h after thawing. Sperm motility was higher in the 5 mM GSH group than in the control or 2.5 and 10 mM GSH groups; this effect was observed at 1 to 24 h after thawing (P < 0.05). The 5 mM GSH group had a higher sperm viability index at 12 and 24 h after thawing compared with the other groups (P < 0.05). Acrosome integrity, evaluated at 4 h after thawing, was greater in two of the GSH-treated groups (5 and 10 mM) compared with the control. Lipid
peroxidation (LP) levels immediately after thawing were lower in the 5 and 10 mM GSH groups compared with the control, while those at 12 h after thawing did not differ significantly. Frozen-thawed semen in the 5 mM GSH group was used for transcervical insemination of 4 bitches, resulting in delivery of 5 puppies from 2 bitches. These results indicate that supplementation of semen extender with 5 mM GSH was effective in improving motility, longevity and acrosomal integrity and inhibiting LP levels in post-thaw canine spermatozoa, without any adverse impacts on full-term development after transcervical insemination. 相似文献
129.
Hiroyuki OHNISHI Shinya MIZUNO Yoko MIZUNO-HORIKAWA Takashi KATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):313-319
Ischemic acute kidney injury (AKI) is the most key pathological event for accelerating
progression to chronic kidney disease through vascular endothelial injury or dysfunction.
Thus, it is critical to elucidate the molecular mechanism of endothelial protection and
regeneration. Emerging evidence indicates that bone marrow-derived cells (BMCs) contribute
to tissue reconstitution in several types of organs post-injury, but little is known
whether and how BMCs contribute to renal endothelial reconstitution, especially in an
early-stage of AKI. Using a mouse model of ischemic AKI, we provide evidence that
incorporation of BMCs in vascular components (such as endothelial and smooth muscle cells)
becomes evident within four days after renal ischemia and reperfusion, associated with an
increase in stromal cell-derived factor-1 (SDF1) in endothelium and that in
CXCR4/SDF1-receptor in BMCs. Notably, anti-CXCR4 antibody decreased the numbers of
infiltrated BMCs and BMC-derived endothelium-like cells, but not of BMC-derived smooth
muscle cell-like cells. These results suggest that reconstitution of renal endothelium
post-ischemia partially depends on a paracrine loop of SDF1-CXCR4 between resident
endothelium and BMCs. Such a chemokine ligand-receptor system may be attributable for
selecting a cellular lineage (s), required for renal vascular protection, repair and
homeostasis, even in an earlier phase of AKI. 相似文献
130.
日本茶树种植导致的环境问题及施用氨晴钙的控制效果 总被引:8,自引:0,他引:8
A field experiment, involving lime N (calcium cyanamide, CaCN2) fertilization as a control measure, was conducted to study environmental problems induced by long-term heavy N application in Japanese tea fields. Long-term tea cultivation caused serious soil acidification. Seventy-seven percent of the 70 tea fields investigated had soil pH values below 4.0, and 9% below 3.0, with the lowest value of 2.7. Moreover, excess N application in tea fields put a threat to plant growth, induced serious nitrate contamination to local water, and caused high nitrous oxide loss. Compared with the conventional high N application treatment (1 100 kg N ha^-1) without lime N, the low N application (400 kg N ha^-1) with calcium cyanamide effectively stopped soil acidification as well as achieved the same or slightly higher levels in tea yield and in total N and amino acid contents of tea shoots. The application of calcium cyanamide could be a suitable fertilization for the prevention of environmental problems in tea cultivation. 相似文献