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111.
Successful production of blastocysts following ultrarapid vitrification with step-wise equilibriation of germinal vesicle-stage mouse oocytes 总被引:4,自引:0,他引:4
Aono N Naganuma T Abe Y Hara K Sasada H Sato E Yoshida H 《The Journal of reproduction and development》2003,49(6):501-506
The purpose of this study was to evaluate the viability and subsequent developmental ability of murine germinal vesicle (GV) oocytes ultrarapidly vitrified after step-wise exposure to cryoprotectants (CPAs). Oocytes were transferred to a vitrification solution composed of 15% ethylene glycol, 15% dimethyl sulfoxide and 0.5 M sucrose in a direct manner (non-preequilibrium) or in a step-wise manner (single-, two-, or ten-step preequilibrium). After ultrarapid vitrification and storage in liquid nitrogen, the oocytes were thawed, washed by diluting the CPAs in five steps, and then subjected to in vitro maturation, fertilization and culture. In the non-preequilibrium group, the rates of post-thawed oocytes surviving, maturing to metaphase-II, developing to blastocysts and to hatching/hatched blastocysts were 91.8, 87.1, 15.9 and 2.3%, respectively. In the single- and two-step groups, the corresponding rates were 97.0-98.2%, 92.2-95.0%, 22.0-29.4% and 8.8-15.6%, whereas in the ten-step group they were 98.2, 91.8, 38.6 and 22.8%, respectively. In the non-vitrified control group, the rates of oocytes maturing to metaphase-II, developing to blastocysts and to hatching/hatched blastocysts were 90.2, 75.2 and 51.5%, respectively. The present study shows that the ultrarapid vitrification of murine GV oocytes by a step-wise manner involving 10 steps preequilibrium may have an advantage in maintaining the viability and subsequent production of blastocysts. 相似文献
112.
Hori T Akikawa T Kawakami E Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(9):807-811
The effects of the long-acting prostagrandin F(2)(alpha)-analogue fenprostalene were investigated. Twenty-three female beagles (24 cases) 1-6 years of age were divided into 6 groups, and a dose of 5-150 microg/head of fenprostalene was subcutaneously administered at 25 days after ovulation to investigate its effects based on peripheral blood progesterone (P(4)) levels. The dogs were also examined for shortening of their estrous cycle after administration and fecundity after the recurrence of estrus. The results showed that the administration of 50 microg/head of fenprostalene or more reduced the levels of peripheral blood P(4) to about 1 ng/ml 2 days after administration, indicating early regression of the corpus luteum, and that the administration of these doses shortened the time to the subsequent estrus by a mean of about 80 days, and that conception rates were normal if estrus recurred about 2 months after fenprostalene administration or later. 相似文献
113.
Inokuma H Fujimoto T Hosoi E Tanaka S Fujisaki K Okuda M Onishi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(7):615-617
Ticks were collected from 94 sika deer (Cervus nippon) hunted in the western part of Yamaguchi Prefecture, Japan from August to November 1999, and March to July 2000. Haemaphysalis longicornis and H. yeni were the dominant species from April to August, while H. flava and H. megaspinosa were dominant in October, November and March. This is the first report of H. yeni in the mainland of Japan. Small numbers of H. kitaokai, Amblyomma testudinarium and Ixodes ovatus were also recorded. 相似文献
114.
Tomoharu Kawahara Kazuhiro Toyoda Akinori Kiba Akiko Miura Takako Ohgawara Mikihiro Yamamoto Yoshishige Inagaki Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2003,69(1):33-38
Two nucleoside triphosphatase (NTPase) cDNA clones were isolated from a cDNA library of Pisum sativum L., cv. Midoriusui. The genes encoding the cDNAs were designated PsAPY1 and PsAPY2. PsAPY1 included the N-terminal amino acid sequence of an NTPase bound to pea cell wall. The phylogenic analysis indicated that PsAPY1 belongs to an NTPase subfamily responsive to environmental stimuli and that PsAPY2 belongs to a discrete subfamily, the physiological role of which is almost unknown. The adenosine triphosphatase activity
of recombinant PsAPY1 was regulated by an elicitor and a suppressor from the pea pathogen Mycosphaerella pinodes. Based on these findings, we discuss the role of NTPases in response to biological stresses.
Received: May 27, 2002 / Accepted: July 31, 2002 相似文献
115.
Kenichi OTAWA Masaaki SATO Takako SASAKI Hiraku SASAKI Jun NONAKA Kikuji ITO Toshio KUROKI Yutaka NAKAI 《Animal Science Journal》2004,75(3):261-269
The ecology of shiga‐toxigenic Escherichia coli (STEC) is important in the animal production environment. We investigated fecal shedding of STEC in one town in Miyagi, Japan by multiplex polymerase chain reaction (PCR) targeting shiga toxin gene 1 (stx1), gene 2 (stx2) and malB promoter gene, and analyzed the PCR products of stx1 or stx2 (54 samples) by direct sequencing. Three of 46 (6.5%) beef cattle in the University Farm of Tohoku University (Kawatabi Farm) and 11 of 70 (15.7%) calves in neighboring dairy farms carried STEC. Rate of detecting genes of stx1, stx2 and stx1+2 was 3.4% (4/116), 8.6% (10/116) and 0.9% (1/116), respectively. Serotyping indicated that STEC contaminated farms at different times or through different routes. Isolates harbored no mutation among stx1, but six (Kawatabi Farm) and 38 (neighboring farms) base substitutions among stx2, respectively. The diversity of substitutions of stx2 was observed among farms or even in a farm. Phylogenic analysis revealed that STEC detected in the area were classified into three clusters by the variety of stx2. Sequence analysis of stx2 will be one of the tools for clarifying the source of outbreaks and the route of contamination of STEC. 相似文献
116.
Daiki KATO Mariko OISHI Koichi OHNO Ko NAKASHIMA Atsuhito WADA Tatsushi MORITA Soichi IMAI Masaya TSUBOI James K. CHAMBERS Kazuyuki UCHIDA Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1499-1502
Ollulanus tricuspis is a small nematode parasite of the
stomach, and its infection has been reported worldwide in cats but only one report in dogs
as post-mortem diagnosis. Two dogs, kept in the Tokyo area, were presented for chronic
vomiting. Chronic gastritis was diagnosed histologically, and many nematodes were detected
in endoscopically-biopsied gastric samples and in the mucus of vomitus in both dogs. The
parasites were small (<1 mm), and their morphological characteristics were consistent
with those previously reported for O. tricuspis. The symptoms in one dog
completely disappeared after anthelmintic therapy. To our knowledge, this is the first
report describing ante-mortem diagnosis of spontaneous gastric O.
tricuspis infection in dogs in which infectivity and pathogenicity of the
nematode are suggested. 相似文献
117.
118.
119.
Kei WATARI Satoru KONNAI Tomohiro OKAGAWA Naoya MAEKAWA Yamato SAJIKI Yukinari KATO Yasuhiko SUZUKI Shiro MURATA Kazuhiko OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):6
Our previous studies demonstrate the therapeutic efficacy against bovine diseases of an anti-bovine programmed death-ligand 1 (PD-L1) chimeric antibody. In humans, PD-1 and PD-L1 antibodies are more effective when combined with an antibody targeting cytotoxic T lymphocyte antigen 4 (CTLA-4) and these combination therapies are therefore clinically used. Here we generated an anti-bovine CTLA-4 chimeric antibody (chAb) to enhance the therapeutic efficacy of the PD-L1 antibody. We further analyzed the effects of dual blockade of CTLA-4 and PD-1 pathways on T-cell responses. The established anti-bovine CTLA-4 chAb showed comparable blocking activity on the binding of bovine CTLA-4 to CD80 and CD86 as the anti-bovine CTLA-4 mouse monoclonal antibody. Anti-bovine CTLA-4 chAb also significantly increased IL-2 production from bovine peripheral blood mononuclear cells (PBMCs). Further, the combination of anti-CTLA-4 chAb with anti-PD-L1 chAb significantly upregulated IL-2 production by PBMCs. These results suggest that the combination of antibodies have higher potential to enhance immune responses against pathogens compared with single administration. 相似文献
120.
Kimura A Fukuda T Zhang M Motoyama S Maruyama N Utsumi S 《Journal of agricultural and food chemistry》2008,56(21):10273-10279
Legume seeds contain 7S and/or 11S globulins as major storage proteins. The amino acid sequences of them from many legumes are similar to each other in the species but different from each other, meaning that some of these proteins from some crops exhibit excellent functional properties. To demonstrate this, we compared protein chemical and functional properties (thermal stability, surface hydrophobicity, solubility as a function of pH, and emulsifying properties) of these proteins from pea, fava bean, cowpea, and French bean with those of soybean as a control at the same conditions. The comparison clearly indicated that the 7S globulin of French bean exhibited excellent solubility (100%) at pH 4.2-7.0 even at a low ionic strength condition (mu = 0.08) and excellent emulsion stability (a little phase separation after 3 days) at pH 7.6 and mu = 0.08, although the emulsions from most of the other proteins separated in 1 h. These results indicate that our assumption is correct. 相似文献