全文获取类型
收费全文 | 329篇 |
免费 | 15篇 |
专业分类
林业 | 14篇 |
农学 | 18篇 |
42篇 | |
综合类 | 16篇 |
农作物 | 15篇 |
水产渔业 | 47篇 |
畜牧兽医 | 174篇 |
园艺 | 7篇 |
植物保护 | 11篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 20篇 |
2020年 | 7篇 |
2019年 | 4篇 |
2018年 | 5篇 |
2017年 | 13篇 |
2016年 | 12篇 |
2015年 | 10篇 |
2014年 | 21篇 |
2013年 | 29篇 |
2012年 | 24篇 |
2011年 | 22篇 |
2010年 | 12篇 |
2009年 | 12篇 |
2008年 | 18篇 |
2007年 | 20篇 |
2006年 | 12篇 |
2005年 | 10篇 |
2004年 | 12篇 |
2003年 | 21篇 |
2002年 | 12篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 8篇 |
1998年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1983年 | 1篇 |
1974年 | 2篇 |
1972年 | 2篇 |
1964年 | 1篇 |
排序方式: 共有344条查询结果,搜索用时 15 毫秒
61.
Shimotori Koichi Yamamoto Haruki Imai Akio Kohzu Ayato Okamoto Takahiro 《Journal of Soils and Sediments》2021,21(2):1222-1231
Journal of Soils and Sediments - Sediment oxygen demand (SOD) measurement currently requires a long preparation time and bulky experimental equipment, which represent major obstacles to conducting... 相似文献
62.
Assessment of the water quality of two rivers in Hanoi City and its suitability for irrigation water
Nguyen Thi Lan Huong Masami Ohtsubo Loretta Li Takahiro Higashi Motohei Kanayama 《Paddy and Water Environment》2008,6(3):257-262
The To Lich and Kim Nguu Rivers in Hanoi City are the main sources of irrigation water for suburban agricultural land and
fish farm. Wastewater from the industrial plants located along these rivers has been discharged, and has degraded the water
quality of the rivers. This study describes the chemical properties of water from the rivers, focusing on heavy metal pollution
and the suitability of water quality for irrigation water. Water from the rivers was heavily polluted with organic matter
and heavy metals such as Pb, Cu, Zn, Cr, Cd and Ni. Dissolved oxygen, chemical oxygen deman, and total suspended solids, and
the concentrations of all heavy metals exceeded the Vietnamese standard for surface water quality in all investigated sites.
The concentrations of some heavy metals such as Cu, Cd, Cr and Ni were above the internationally recommended WHO maximum level
for irrigation water. A wide variation in the heavy metal concentration of water due to metal types is the result of wastewater
discharged from different industrial sources. 相似文献
63.
Masato MIYAKE Shinichiro HAYASHI Yoshikazu TAKETA Shunsuke IWASAKI Kouichi WATANABE Shyuich OHWADA Hisashi ASO Takahiro YAMAGUCHI 《Animal Science Journal》2010,81(2):223-229
Myostatin (MSTN) is a negative regulator during muscle differentiation, whereas insulin‐like growth factors (IGFs) are essential for muscle development. MSTN and IGFs act oppositely during myogenesis, but there is little information on the mutual relationship of MSTN and IGFs. The present study was conducted to examine whether MSTN affects IGF expression during early myogenesis in cattle. IGF‐1 mRNA was similarly expressed in M. longissimus thoracis of double‐muscled (DM) and normal (NM) Japanese shorthorn cattle. IGF‐2 mRNA expression was consistently higher in the normal and regenerating muscle of DM cattle than those of NM cattle. When myoblasts were isolated from regenerating M. longissimus thoracis, IGF‐2 mRNA expression showed a significant increase in differentiating DM derived myoblasts (DM‐myoblasts) as compared with differentiating NM derived myoblasts (NM‐myoblasts). An addition of recombinant mouse myostatin (rMSTN) to myoblast cultures attenuated IGF‐2 mRNA expression and decreased myotube formation, but did not effect IGF‐1 mRNA expression. An activin‐like kinase (ALK) inhibitor, SB431542, mediates MSTN action, suppressed the translocation of Smad2/3 into the nucleus in DM‐myoblasts, and restored the attenuated IGF‐2 mRNA expression and the decreased myotube formation induced by rMSTN in myoblast cultures. The findings indicate that MSTN may negatively regulate myoblast differentiation by suppressing IGF‐2 expression via ALK‐Smad signaling. 相似文献
64.
H Yokota A Yuasa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(1):105-111
UDP-glucuronyltransferase activity toward xenobiotics in rat liver microsomes was increased about 2.6-fold by administration of a eugenol (4-allyl-2-methoxyphenol). Km value of the induced enzyme toward UDP-glucuronic acid, however, did not change. Immunoblotting analysis revealed that the amount of UDP-glucuronyltransferase protein was increased in the microsomes of eugenol-treated rat liver. In vitro translation assay showed that the level of translatable mRNA encoding this enzyme increased in the liver. These results indicate that mRNA specific for production of UDP-glucuronyltransferase has accumulated, presumably by de novo synthesis in response to a plant phenol, eugenol. 相似文献
65.
66.
Komei HOTTA Takayuki WATANABE Chiho KISHIDA Yukio NAKAMURA Nobuyuki OHKUBO Takahiro MATSUBARA Shinji ADACHI Kohei YAMAUCHI 《Fisheries Science》2003,69(3):555-562
ABSTRACT: Because blood vitellogenin (Vg) has been considered a biomarker for environmental estrogens, the basal levels of Vg and 17β-estradiol (E2 ) were determined in male Japanese whiting reared under natural conditions. Serum levels of Vg and E2 were measured and gonadal development was assessed by gonadosomatic index (GSI) and histological observation in 8–10 male fish at monthly intervals throughout the annual reproductive cycle. Serum E2 was <60 pg/mL throughout the study period. In contrast, serum Vg exhibited seasonal changes: serum levels of Vg gradually increased from April to May (mean 63 ± 13 ng/mL and 124 ± 48 ng/mL in April and May, respectively), and then reached a peak value (mean 352 ± 68 ng/mL) in June. Thereafter, serum Vg gradually decreased, reaching undetectable levels (<50 ng/mL) in October. Serum levels of Vg tended to increase in the male fish in which the GSI was >1%. Histological observation revealed that testes in such male fish were in active spermatogenesis and then all of the testes of male fish in which serum Vg decreased to ND levels were regressed. These results suggest that Vg productive potency (sensitivity to estrogens) may increase in the spermatogenic stage, resulting in production of Vg in response to very low levels of natural or xenobiotic estrogens. 相似文献
67.
Fumie Kawasaki Ioanna Katsiadaki Alexander P. Scott Takahiro Matsubara Kiyoshi Osatomi Kiyoshi Soyano Akihiko Hara Koji Arizono Masaki Nagae 《Fish physiology and biochemistry》2003,28(1-4):425-425
Two types of spiggin cDNAs were isolated from the three-spined stickleback. Northern blot analysis revealed that glue protein is composed of multiple spiggin molecules, and that their synthesis in kidney is strongly up-regulated by androgens. 相似文献
68.
Yoshikazu TAKETA Yasuhiro NAGAI Hideki OGASAWARA Shinichiro HAYASHI Masato MIYAKE Sachi TANAKA Kouichi WATANABE Shyuichi OHWADA Hisashi ASO Takahiro YAMAGUCHI 《Animal Science Journal》2008,79(3):382-390
Myostatin (MSTN), known as growth and differentiation factor 8 (GDF-8), is a member of the transforming growth factor β (TGF-β) superfamily that negatively regulates skeletal muscle mass. Myostatin binds with high affinity to the receptor serine threonine kinase activin receptor type IIB (ActRIIB). Activins that also belong to the TGF-β superfamily, stimulate follicle-stimulating hormone production in gonadotrophs and suppress growth hormone and adrenocorticotropic hormone production in somatotrophs and corticotrophs, respectively. The aim of the present paper was therefore to clarify the endocrine action of MSTN in adenohypophysis. The present study details the expression and cellular localization of MSTN and ActRIIB in porcine anterior pituitary gland. The mRNA of MSTN and ActRIIB was consistently expressed in RT-PCR. Immunohistochemistry of MSTN and specific hormones showed that MSTN localized in thyrotrophs and gonadotrophs, in which most of the MSTN immunoreactive cells were identified as thyrotrophs. The immunostaining of ActRIIB was restricted to corticotrophs. These results indicate that MSTN was mainly produced in thyrotrophs and its receptor, ActRIIB, was restrictively contained in corticotrophs. Interestingly, thyrotrophs immunoreactive for MSTN were frequently close to corticotrophs immunoreactive for ActRIIB. The present study suggests that MSTN from thyrotrophs may regulate corticotroph function as a paracrine mediator among the porcine anterior pituitary cells. 相似文献
69.
Kangmin Seo Takahiro Suzuki Ken Kobayashi Takanori Nishimura 《Animal Science Journal》2019,90(3):423-434
The development of adipose tissue in skeletal muscle is important for improving meat quality. However, it is still unclear how adipocytes grow in the proximity of muscle fibers. We hypothesized that adipocytes would suppress muscle cell growth so as to grow dominantly within muscle. In this study, we investigated the effect of adipocytes on the differentiation of muscle cells in a co‐culture system. The fusion index of C2C12 myoblasts co‐cultured with 3T3‐L1 adipocytes was significantly lower than that of the control. The expression of myogenin and myosin heavy chain in C2C12 muscle cells co‐cultured with 3T3‐L1 adipocytes was significantly lower than in the control. Furthermore, the expression of Atrogin‐1 and MuRF‐1 was higher in C2C12 muscle cells co‐cultured with 3T3‐L1 adipocytes than the control. These results suggest that 3T3‐L1 adipocytes suppress the differentiation of C2C12 myoblasts. In addition, 3T3‐L1 adipocytes induced the expression and secretion of IL‐6 in C2C12 muscle cells. The fusion index and myotube diameter were higher in C2C12 muscle cells co‐cultured with 3T3‐L1 cells in medium containing IL‐6‐neutralizing antibody than the control. Taken together, there is a possibility that adipocyte‐induced IL‐6 expression in muscle cells could be involved in the inhibition of muscle cell differentiation via autocrine. 相似文献
70.