人工圈养对短尾猴的影响初报

比较安徽黄山鱼鳞坑群短尾猴人工圈养前后的种群生态学和行为学资料表明，人工圈养：（１）降低了个体的迁入率和迁出率，增加了幼体的出生率和幼体死亡率，结果使种群的年平均增长率下降；（２）加速了猴群的分群进程；（３）使个体间的友好行为频率下降，社会联系减弱；同时，攻击行为频率上升，导致群体结构松散。文中分析了人工圈养中人工投饵、人为地限制猴群活动空间范围和游人对上述诸问题的影响机制，并提出了改善和加强管理的措施。     

蝴蝶兰组织培养中的褐化控制研究

 系统研究了吸附剂(活性炭、PVP) 与柠檬酸、半胱氨酸、谷胱甘肽、维生素C和硫代硫酸钠5种抗氧化剂对蝴蝶兰组培中褐化的影响。结果表明: 活性炭能有效控制褐化和促进生长, 但不利于分化; 谷胱甘肽控制褐化的效果虽不及活性炭, 但综合效果最佳。     

一株新的化能无机营养菌——聚积碳酸镁的细菌

从新疆吐鲁番盆地分离了一株新的化能无机营养菌Tp892 4,其主要特征是聚积碳酸镁 ,只在镁盐和碳酸钠的培养基中迅速生长繁殖。细胞球形 ,成串而有规则地排列在碳酸镁的晶体中。菌落白色、坚硬、中间隆起向外散射形成雪花状。细菌能在 10～ 75℃的温度范围和 8～ 14的 pH范围内生长。致死温度 10 0℃ 5 0′ ,平均代时 0 .5hr。这与“伯杰细菌鉴定手册”和“伯杰系统细菌手册”中描述的化能无机营养菌有明显的差别 ,至今国内外尚未见过报道。根据这些特性 ,作者认为该菌是一种新的聚积碳酸镁的化能无机营养菌 ,暂命名为吐鲁番聚镁球菌Polymagcoccusturpanensissp .nov .     

20%多效唑·甲哌 FEE7 微乳剂防止小麦倒伏和增产机理研究

冬小麦二棱期喷施植物生长调节剂20%多效唑·甲哌鎓微乳剂375 mL/hm2,可以显著抑制茎秆基部节间伸长,增加各节间充实度,其中赤霉素(Gas)和生长素(IAA)降低,可显著增强小麦抗倒伏能力和降低田间倒伏率.处理还协调了穗数、穗粒数和粒重的关系,增产幅度6.2%～28.6%.增产原因可能在于促进籽粒灌浆强,增加籽粒中内源Gas、IAA、细胞分裂素(CTKs)的水平,增强了籽粒库活性,同时促进茎叶中干物质向籽粒运转.     

20%多效唑·甲哌Wong微乳剂防止小麦倒伏和增产机理研究

冬小麦二棱期喷施植物生长调节剂20%多效唑·甲哌鎓微乳剂375 mL/hm2,可以显著抑制茎秆基部节间伸长,增加各节间充实度,其中赤霉素(Gas)和生长素(IAA)降低,可显著增强小麦抗倒伏能力和降低田间倒伏率.处理还协调了穗数、穗粒数和粒重的关系,增产幅度6.2%～28.6%.增产原因可能在于促进籽粒灌浆强,增加籽粒中内源Gas、IAA、细胞分裂素(CTKs)的水平,增强了籽粒库活性,同时促进茎叶中干物质向籽粒运转.     

大豆疫霉根腐病菌毒素产生条件的研究

 大豆疫霉根腐病(Phytophthora sojae)是大豆的重要病害之一,危害大,发展快。自1918年首次在美国印弟安那州发现此病以来、相继在澳大利亚、加拿大,匈牙利等国发生。     

地高辛标记核酸探针的标记方法及其在食用菌研究领域的应用

综述了非放射性地高辛（DIG）标记系统的原理和主要特点，介绍了地高辛标记核酸探针的主要标记方法，影响探针标记方法选择的因素，标记探针的显色检测方法及其在食用菌研究领域的应用。     

High efficient expression of human endostatin in E.coli and its antiangiogensis activity

AIM: To examine the expression of human endostatin in E.coli, produce its fusion protein antibody and observe its biological activity. METHODS: Endostatin gene was amplified by polymerase chain reaction,recombined with plasmid vector pGEX-2T and induced expression with IPTG.The protein activity was tested by endothelial cell proliferation inhibitory assay.Inclusion body crudely purified was used to generate polyclonal antibody to detect its expression at mouse's liver and kidney etc. RESULTS: The protein expressed was 20kD after digestion by thrombin,it appeared the anti-angiogenesis activity and Western blotting indicated the expression of endostatin in liver and kidney of mouse. CONCLUSION: The successful expression of human endostatin and the preparation of polycolonal antibody indicated its potential application in anti-angiogenesis therapy and diagnosis tumors.     

Effects of carvedilol,cilazapril and their combination on left ventricular remodeling after acute myocardial infarction in rats

AIM:To compare the effects of carvedilol, cilazapril and their combination on left ventricular remodeling(LVRM) after acute myocardial infarction(AMI) in rats. METHODS: Twenty-four hours after AMI operation, 100 surviving rats were randomly assigned to: ①AMI control(n= 25), ②AMI+carvedilol(1 mg·kg^-1 ·d^-1, n= 25)(C1), ③AMI+cilazapril(1 mg·kg^-1 ·d^-1, n= 25)(Z1), and ④ AMI+combination(n= 25) groups. Sham-operated group(n= 17) were selected randomly. After 4 weeks of therapy with the drugs gastric gavage, hemodynamic and pathological studies were performed. RESULTS: There were no significant differences in MI size among the four AMI groups(all P> 0.05) Left ventricular(LV) end diastolic pressure(LVEDP), volume(LVV), weight(LVW) and septal thickness(STh) were all higher and left ventricular pressure maximal rate of rise and fall(±d p /d t) were lower(all P< 0.01) in AMI group than sham-operated group. The LVEDP, LVV, LVW and STh were all lower and ±dp /dt were higher in Z1, C1, and combination groups than those in AMI group(P< 0.05, P< 0.01), with LVEDP and STh were more lower in the combination group than in the two monotherapy group(P< 0.05, P< 0.01), but there were no significant differences in other variables among the three therapy groups. CONCLUSION: Carvedilol, cilazapril and their combination all can prevent from LVRM after AMI in rats, improve hemodynamics and LV function, with the combination superior.