Electroacupuncture-induced neural activation detected by use of manganese-enhanced functional magnetic resonance imaging in rabbits

OBJECTIVE: To investigate the effects of acupuncture on neural activity detected by use of manganese-enhanced functional magnetic resonance imaging (fMRI) and elucidate the relationship between somatic acupoint stimulation and brain activation. ANIMALS: 40 New Zealand White rabbits. PROCEDURE: Manganese-enhanced fMRI was performed in anesthetized rabbits manipulated with electroacupuncture (EA) on Zusanli (ST-36) and Yanglingquan (GB-34) acupoints. Image acquisition was performed on a 1.5T superconductive clinical scanner with a circular polarized extremity coil. T1-weighted images were acquired sequentially as follows: baseline, after mannitol injection, after manganese infusion, and 5 and 20 minutes after initiation of EA. RESULTS: Changes in focal neural activity were detected by use of manganese-enhanced fMRI. Stimulation on Zusanli (ST-36) for 5 minutes resulted in activation of the hippocampus, whereas stimulation on Yanglingquan (GB-34) resulted in activation of the hypothalamus, insula, and motor cortex. Activation became less specific after 20 minutes of EA. Furthermore, stimulation on ipsilateral acupoints led to bilateral brain activation. CONCLUSIONS AND CLINICAL RELEVANCE: Each acupoint has a corresponding cerebral linkage, and stimulation on these points resulted in time-dependent neural activation. Understanding the linkage between peripheral acupoint stimulation and central neural pathways may provide a useful guide for clinical applications of acupuncture.     

成年温州蜜柑夏季高温带叶高接换种技术研究课题通过成果鉴定

由江西省安福县果业局和安福县洲湖于英园艺场共同实施的成年温州蜜柑夏季高温带叶高接换种技术研究课题，于2005年12月7日通过江西省吉安市科技局组织的科技成果鉴定。专家一致认为，该课题的研究成果达国内同类研究先进水平，填补了国内柑桔夏季高温高接的空白，为柑桔类果树高接换种开辟了新的途径。     

RT-PCR技术检测猪瘟病毒的应用研究

本试验应用反转录-聚合酶链式反应(RT-PCR)对猪瘟进行诊断应用研究.应用RT-PCR对来自广西不同地区的135份疑似猪瘟病料进行检测,84份诊断为阳性,阳性率62.2%.从百色、柳州地区等采集的健康猪扁桃体和淋巴结共276份,经RT-PCR检测,37份为阳性,阳性率为13.4%.其中健康猪扁桃体带毒较高,246份扁桃体中有35份阳性,占14.2%.采自柳州健康猪的26份淋巴结材料全为阴性,只有邕宁县的1份健猪淋巴结阳性.结果表明,RT-PCR技术可应用于猪瘟的临床诊断.     

关于混凝土泵送施工技术的研究探讨

当前,泵送混凝土技术在我国得到了较为广泛的应用。文章针对混凝土的泵送施工技术问题,从可泵性混凝土的配料、混凝土输送泵的选型和布置、现场输送管道的敷设、混凝土的输送和管道堵塞原因及防治措施等方面做了分析探讨。     

优良野生牧草毛花雀稗生育特性研究

对野生毛花雀稗不同分株种植处理的成活率、分蘖力、开花结实和根系分布情况进行测定分析,得出以下结论成活率以处理2(两个分蘖植株)最高,为100%;各处理总分蘖数相差不大;处理2和处理3(两个分蘖植株放置10 d)开花结实情况较好,处理1(单个分蘖植株)最差;根系分布情况差异不显著.另对处理2进行了生长速度和再生速度测定,毛花雀稗的生长速度在7月8～15日之间出现峰值,为5.1 cm/d,峰值前半段为保持较低生长水平缓慢增长,5月份后生长开始加快.峰值后半段为保持较高的生长水平缓慢下降趋势,进入11月后生长水平急剧下降.     

Intriguing interplay between viral proteins during herpesvirus assembly or: the herpesvirus assembly puzzle

Herpes virions are complex particles that consist of more than 30 different virally encoded proteins. The molecular basis of how this complicated structure is assembled is only recently beginning to emerge. After replication in the host cell nucleus viral DNA is incorporated into preformed capsids which leave the nucleus by budding at the inner nuclear membrane resulting in the formation of primary enveloped virions in the perinuclear space. The primary envelope then fuses with the outer leaflet of the nuclear membrane, thereby releasing nucleocapsids into the cytoplasm. Final envelopment including the acquisition of more than 15 tegument and more than 10 envelope (glyco)proteins occurs by budding into Golgi-derived vesicles. Mature virions are released after fusion of the vesicle membrane with the plasma membrane of the cell. Thus, herpesvirus morphogenesis requires a sequence of envelopment--de-envelopment--re-envelopment processes which are distinct not only in the subcellular compartments in which they occur but also in the viral proteins involved. This review summarizes recent advances in our understanding of the complex protein-protein interactions involved in herpesvirus assembly and egress.     

新生犊牛疾病的诊治措施

新生犊牛出生后,出于种种原因导致某种症状的发生。如不及时采取正确适当的治疗措施,会常常导致新生犊牛的死亡。     

关于种猪生产企业营销服务的几点思考

改革开放20多年来,国内养猪业得到了迅猛发展,种猪生产作为养猪产业链条中的关键环节,对养猪业的发展与猪的品种改良起到了至关重要的作用.种猪生产企业不仅要精心培育高品质的种猪,还要把优质的种猪产品推广到社会,得到广大养殖户的认可,力争创造最大的社会效益.目前种猪生产企业众多,种猪销售市场的竞争日趋激烈,但是种猪品质参差不齐.     

矮小型褐壳蛋鸡蛋白质和含硫氨基酸需要量的研究：II代谢试验和 …

将２７０只矮小型褐壳蛋鸡随机分为９组，饲喂９种不同蛋白质和含硫氨基酸水平日粮。９种试验日粮分别含有１１．５０ＭＪ／ｋｇ代谢能和１６．５２％、１７．２９％、１８．１７％粗蛋白质以及０．６２％、０．６６％、０．７２％含硫氨基酸，研究矮小型褐壳蛋鸡蛋白质和含硫氨基酸的需要量。代谢试验结果表明，４０周龄矮小型褐壳蛋鸡每日采食１４．１３ｇ蛋白质和０．５５８ｇＳ－氨基酸时，氮存留量达到最大值。血浆游离氨基酸测     

Use of echocardiography for the diagnosis of heartworm disease in cats: 43 cases (1985-1997)

OBJECTIVE: To determine the usefulness of echocardiography in the diagnosis of heartworm disease in cats and to compare this modality with other tests. DESIGN: Retrospective study. ANIMALS: 43 cats with heartworm infection that had echocardiographic examinations at 2 veterinary teaching hospitals between 1985 and 1997. Twenty-two of these 43 cats also underwent radiography of the thorax and heartworm antibody and heartworm antigen testing. PROCEDURE: Cats were determined to be infected with Dirofilaria immitis infection on the basis of 1 or more of the following findings: positive modified Knott or antigen test result, echocardiographic evidence of heartworm disease, or confirmation of the disease on postmortem examination. The percentage of echocardiographs in which heartworms were evident was compared with the percentage of radiographs in which pulmonary artery enlargement was evident and results of antigen or antibody tests in cats in which all tests were performed. RESULTS: Overall, heartworms were detectable by use of echocardiography in 17 of 43 cats, most often in the pulmonary arteries. In the 22 cats in which all tests were performed, antibody test results were positive in 18, antigen test results were positive in 12, and pulmonary artery enlargement was evident radiographically and heartworms were identifiable echocardiographically in 14. Heartworm infection was diagnosed exclusively by use of echocardiography in 5 cats in which the antigen test result was negative. CONCLUSIONS AND CLINICAL RELEVANCE: Although echocardiography was less sensitive than antigen testing, it was a useful adjunctive test in cats that had negative antigen test results in which there was a suspicion of heartworm disease. The pulmonary arteries should be evaluated carefully to increase the likelihood of detection of heartworms echocardiographically.