Toxoplasmosis in two cats with inflammatory intestinal disease

Lymphocytic-plasmacytic enteritis, a chronic inflammatory intestinal disease, was diagnosed in 2 cats. In 1 cat, recurrence of clinical signs after initiating treatment was attributed to relapse of the inflammatory intestinal disease, but was found to be attributable to relapsing toxoplasmosis secondary to immunosuppressive drug therapy. Treatment with clindamycin resolved the recurrent toxoplasmosis. In the second cat, clinical signs of toxoplasmosis did not develop, but serologic testing yielded evidence of active toxoplasmosis. Treatment with clindamycin caused the titers to decrease. Relapsing toxoplasmosis may be responsible for apparent resistance to treatment in cats for inflammatory intestinal disease being treated with immunosuppressive drugs.     

Influence of ectomycorrhization and cesium/potassium ratio on uptake and localization of cesium in Norway spruce seedlings

Norway spruce (Picea abies (L.) Karst.) seedlings growing in a growth pouch system were used to investigate the effects of the ectomycorrhizal fungus Hebeloma crustuliniforme (Bull. ex St. Amans) Quél. and various Cs/K ratios on the uptake of (134)Cs, expressed as a percentage of the total amount of (134)Cs supplied. The amount of (134)Cs taken up by seedlings increased with increasing Cs/K ratio. At a Cs/K ratio of 0.1, uptake of (134)Cs ranged between 7.2 and 7.3% and was independent of ectomycorrhizal status, whereas at Cs/K ratios >/= 1 uptake of (134)Cs varied from 8.1 to 11.1% for ectomycorrhizal and from 10.4 to 14.4% for non-inoculated plants. Ectomycorrhizal seedlings contained a lower concentration of (134)Cs than non-inoculated seedlings. Among plant parts, the amount of (134)Cs was significantly lower in needles and lateral roots of ectomycorrhizal seedlings compared with non-inoculated seedlings. Among fungal and seedling tissues, highest X-ray net counts of (133)Cs were measured in fungal hyphae of ectomycorrhizal mantles. X-Ray net counts of (133)Cs in lateral roots of ectomycorrhizal and non-inoculated plants were similar, but 5 to 10 times higher than in main roots and needles, suggesting an accumulation of (133)Cs in lateral roots and slow translocation to other plant parts. In contrast, X-ray net counts of K indicated that K was readily mobilized from lateral roots to main roots and needles. Elemental mapping showed a relatively homogeneous distribution of (133)Cs within the root.     

Hepatomas in Atlantic tomcod Microgadus tomcod (Walbaum) collected in the Hudson River estuary in New York

Abstract. Incidental observations of Atlantic tomcod during routine laboratory processing revealed that a portion of the adult population collected during the 1977–78 spawning season had enlarged livers containing dark coloured tumours and other abnormalities. Of the total of 264 livers collected between 16 January and 27 February 1978 and grossly examined for prevalence of abnormalities, 25% appeared to contain neoplastic nodules and hepatocellular carcinoma. One liver contained a massive tumour (7 × 12 mm) that involved approximately 60% of the liver. The exact causes of the high prevalence of hepatocellular carcinoma are unknown but poly-chlorinated biphenyls (PCBs) are suspected of having a possible role. The Hudson River is known to contain elevated concentrations of PCBs. Twelve tomcod livers from the 1977–78 spawning population representing both normal and hepatoma conditions contained concentrations of PCBs ranging from 10–9 to 98–2 ppm (mean of 37–5 ppm).     

Antigen competition: antigens compete for a cell occurring with limited frequency

Experimentally altered ability of transferred spleen cells to generate hemolytic plaque-forming cells provided evidence that antigens compete for a type of multipotential cell that contributes to the formation of immunologically competent units. De'ay of exposure of transferred spleen cells to antigen provided results which suggest that different types of cells interact to form competent, antigen-reactive units even in the absence of antigen.     

Factors affecting the variability in cracked and dirty eggs and egg production profitability on an integrated poultry operation

1. A number of factors causing variability in the percentages of cracked, and dirty eggs and weekly standard margin (WSM) of returns from eggs over production costs were examined on an integrated commercial poultry plant in Scotland.

2. As the bird aged, the percentage of cracked eggs increased (P <0.01) and the percentage of grade A eggs and WSM decreased (P <0.01). Cracks in dirty eggs (10.1%) were more than double those in all eggs laid (4.9%).

3. Seasonal effects on percentage cracked eggs and percentage grade A eggs were one‐third the magnitude of those due to age but were significant (P <0.05).

4. Variability in percentage cracked eggs, dirty eggs and WSM accounted for by the factors measured, were 72, 64 and 92% respectively.

5. The variability in WSM was significantly affected by percentage lay, food consumption and age but not by percentage cracked eggs, originally dirty and grade A eggs.

6. Flock management and climate inside the laying house each increased the amount of variability accounted for in the percentage cracked eggs and dirty eggs by at least 10%.     

Preliminary evaluation of diagnostic tests using horses experimentally infected with trypanosoma evansi

Seven surra negative horses were intravenously inoculated with 3 x 10(6)Trypanosoma evansi parasites derived from a camel. One horse was maintained as an uninfected negative control. Three antigen and three antibody detection tests were evaluated for diagnosis of infection in horses. The microhaematocrit centrifugation test (MHCT) was the most sensitive, first detecting parasites between one and three days (x 2.4) post infection (p.i.). The antigen (ag)-ELISA detected antigen between three and ten days (x 6.6) p.i. The latex agglutination test (LAT) first gave positive results on day 3 (x 3.0) p.i. Following the treatment of horses with trypanocidal drugs, the MCHT and the mouse inoculation test (MIT) became negative. Antigen levels using LAT declined and reached pre-infection levels in five out of six horses during the period of observation (92-279 days). Antigen levels using the ag-ELISA declined as well but did not reach pre-infection levels in any of the six horses.Three antibody detection techniques, ab-ELISA, card agglutination test (CATT), and immunofluorescent antibody test (IFAT) detected antibodies in the blood of all seven infected horses but not in the uninfected control. However, the ab-ELISA did not discriminate clearly between sera from infected and uninfected horses because unacceptably high ELISA background readings were detected in 15% of the surra negative horses shipped to the UAE from the UK. The ELISA antibody increased above pre-infection levels in the six horses experimentally infected, but not in one horse. In this horse the ELISA antibody level exceeded the cut-off level only after the reoccurrence of the T. evansi infection. The IFAT detected antibodies 15.7 days p.i. in all infected horses.