Effect of glyphosate on the tripartite symbiosis formed by Glomus intraradices,Bradyrhizobium japonicum,and genetically modified soybean

Most soybeans grown in North America are genetically modified (GM) to tolerate applications of the broad-spectrum herbicide glyphosate; as a result, glyphosate is now extensively used in soybean cropping systems. Soybean roots form both arbuscular mycorrhizal (AM) and rhizobial symbioses. In addition to individually improving host plant fitness, these symbioses also interact to influence the functioning of each symbiosis, thereby establishing a tripartite symbiosis. The objectives of this study were to (1) estimate the effects of glyphosate on the establishment and functioning of AM and rhizobial symbioses with GM soybean, and (2) to estimate the interdependence of the symbioses in determining the response of each symbiosis to glyphosate. These objectives were addressed in two experiments; the first investigated the importance of the timing of glyphosate application in determining the responses of the symbionts and the second varied the rate of glyphosate application. Glyphosate applied at recommended field rates had no effect on Glomus intraradices or Bradyrhizobium japonicum colonization of soybean roots, or on soybean foliar tissue [P]. N₂-fixation was greater for glyphosate-treated soybean plants than for untreated-plants in both experiments, but only when glyphosate was applied at the first trifoliate soybean growth stage. These data deviate from previous studies estimating the effect of glyphosate on the rhizobial symbiosis, some of which observed negative effects on rhizobial colonization and/or N₂-fixation. We did observe evidence of the response of one symbiont (stimulation of N₂-fixation following glyphosate) being dependent on co-inoculation with the other; however, this interactive response appeared to be contextually dependent as it was not consistent between experiments. Future research needs to consider the role of environmental factors and other biota when evaluating rhizobial responses to herbicide applications.     

Some properties of alfalfa mosaic virus isolated from Buddleia davidii in the UK

Alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV) were isolated from clones of Buddleia davidii collected at Long Ashton Research Station for assessment of horticultural characteristics. The AMV isolate investigated in this study (AMV-B) infected 18 species and cultivars of herbaceous test plants. The in vitro properties of AMV-B were determined. The virus was purified by permeation chromatography on controlled-pore glass beads and an antiserum prepared. AMV-B appeared to be serologically indistinguishable from AMV isolate 15/64. The sizes and shapes of the virus particles were those of classical AMV components. The capsid protein was a single polypeptide of Mr 24 375. Buddleia seedlings inoculated with AMV-B showed only mild chlorosis and slight distortion of leaves.     

Control of Glomerella cingulata f.sp. camelliae with fungicides

A critical test of fungicidal activity against the wound pathogen Glomerella cingulata f.sp. camelliae on camellias involved the application of a conidial suspension to scratches on detached leaves, treated with fungicide 24 h previously. In this test, prochloraz at 250–500 μg/ml a.i. in both EC and manganese WP formulations was highly effective, inhibiting leaf rotting by 90–100% compared with 55% or less in some tests with benomyl, chlorothalonil and captafol at 500–1000 μg/ml and captan at 1200 μg/ml. Prochloraz gave similar protection to leaves when applied to whole plants. Protection was less, especially on older leaves, when wounding and inoculating were delayed by 7 or more days after treatment. Prochloraz did not prevent all infection through comparatively large wounds, e.g. leaf scars, made on stems 24 h after treatment.
Poor control of the disease in a nursery was not due to resistance of the pathogen to benomyl; in vitro tests failed to detect full resistance at this time. Under nursery conditions a statutory policy of destroying infected plants and spraying the remainder with prochloraz or prochloraz manganese (both at 500 μg/ml), alternating with benomyl (500 μg/ml) on a weekly basis apparently prevented spread of the disease to healthy plants.     

On difficulties with allelopathy methodology

Summary. The main techniques and the common experimental methods used in studying allelopathy are reviewed. Problems associated with both methodology and conclusions drawn based on certain techniques are discussed. The accuracy obtained using these techniques is evaluated and show that some of the techniques could be a misleading tool. Some changes are suggested.     

The significance of VA-Mycorrhiza to future arable farming in the Netherlands

Present conditions of high inputs and narrow rotations render the importance of vesicular-arbuscular mycorrhiza (VAM) in arable farming in the Netherlands rather low. Changes expected in the near future under the influence of economic pressures and environmental problems may change this situation. This is discussed in the light of some such expected changes in Dutch arable farming.     

Designing resistance management programs: How can you choose?

A list of often-discussed resistance management tactics is critically reviewed. Alteration of insecticides across generations, non-persistent formulations, and the use of pesticides conferring only low magnitudes of resistance seem to be among the most promising tactics for general use in resistance management. Justifying the first two choices usually requires little more than data on product persistence in terms of toxic effect on susceptible individuals under field conditions. The last tactic requires additional information on resistance ratios for resistant and susceptible genotypes under field exposure. The efficacy of pesticide mixtures is highly dependent on equal persistence of both compounds used in the mixture. The number of genes involved in resistance and the fitness disadvantages they may confer in the absence of use appear to be of relatively little significance in choosing management tactics.     

The use of entomogenous fungi for pest control and the role of toxins in pathogenesis

Fungi have been suggested as agents for the biological control of insects for over a century, but their use remains extremely limited. This paper examines the biology of entomogenous fungi, highlighting their need for moisture, and reviews studies on the Entomophthoraceae, Beauveria bassiana, Beauveria brongniartii, Metarhizium anisopliae and Verticillium lecanii. It is suggested that current studies should be directed towards the control of pests living in humid micro-climates and that moisture-retaining formulations should be developed to reduce the reliance of fungi on moisture. Many entomogenous fungi produce insecticidal toxins in submerged culture and these compounds are of interest as a source of new toxophores. The most extensively studied toxins are the cyclic depsipeptides from M. anisopliae, termed destruxins, and their possible role in pathogenesis is discussed. Beauveria bassiana also produces the cyclic peptides termed beauvericin, beauverolides, and bassianolide. Toxins produced by V. lecanii, and the Entomophthoraceae are also described.     

Diclofop-methyl tolerance in cultivated oats (Avena sativa L.)

The feasibility of achieving selective wild oat (Avena fatua L.) control in a stand of the Australian oat (Avena sativa L.) cv. Savena I was examined. Diclofop-methyl was applied at rates of 0·4–0·7 kg a.i. ha^?1 at the three- and five-leaf stages of A. fatua (84–132 culms m²). Although all rates of diclofop-methyl caused initial chlorosis and necrosis to the crop, the subsequent control of A. fatua permitted increased crop tillering. Avena fatua control and crop yield response were maximized when diclofop-methyl was applied at the three-leaf stage. Crop grain yield was increased by as much as 32% and 22% during 1984 and 1985, respectively. In the absence of A. fatua competition, crop shoot dry weight at ear emergence was reduced only where diclofop-methyl was applied at 0·6 or 0·7 kg ha^?1. However, final grain yield was not reduced by any treatment. Only 11 of 240 cultivars or lines tolerated diclofop-methyl, and none reached the level attained by Savena I and a closely related Australian line [(Irwin × (West × New Zealand Cape/42)) × West]/24. The nature of the inheritance of diclofop-methyl tolerance was examined (1983–1985) by crossing and backcrossing (BC) Savena I with four diclofop-methyl susceptible, but agronomically superior, lines. Inheritance of diclofop-methyl tolerance appeared to be controlled by two genes, with susceptibility being dominant to tolerance in F₃, BCIF₂ and BCIF₃ lines.     

Pythium oligandmm associated with a cropping disorder of Agaricus bisporus

Pythium oligandrum was consistently isolated from black patches of mushroom compost from two farms. When used in experiments it inhibited the growth of mycelium of Agaricus bisporus and this effect was enhanced in the presence of a compost supplement which contained high levels of nitrogen. The common name pythium-black compost is proposed for the problem.