Factors affecting the natural transmission of bovine leukaemia virus infection in Queensland dairy herds

Natural transmission of bovine leukaemia virus (BLV) infection in south-eastern Queensland dairy herds was slow in 2 herds with a low to moderate (13 to 22%) prevalence of infection. Infection spread much more rapidly in a herd that had a higher prevalence (42%) when first tested. In a 13 month study of this herd, the cumulative incidence of infection was 24%. In one herd new infections were confined almost entirely to calves of uninfected dams. Following the end of feeding bulk milk to calves, a common practice in dairy herds, no more calves in this herd became infected. In laboratory experiments, neither prolonged housing of susceptible calves with infected cattle, consumption of drinking water contaminated with infected blood, nor inoculation of sheep with saliva from infected cattle resulted in transmission of BLV infection. Sheep were infected by subcutaneous inoculation of a suspension of purified lymphocytes from an infected heifer. The minimum infective dose was 10(3) lymphocytes, equivalent to the number of lymphocytes in approximately 0.1 microliter blood. Thus, procedures involving the transfer of a very small volume of blood from animal-to-animal have the potential to transmit infection.     

Thyroid activity in relation to feed intake, growth rate and feed conversion in growing heifers

Phenotypic and genetic parameters for thyroid activity (T4 serum level, T4 apparent distribution volume, T4 fractional turnover rate and T4 degradation), and for certain production traits (roughage dry matter intake, daily weight gain and roughage dry matter/kg weight gain), were investigated in cattle. In the experiment, 480 growing heifers were studied, daughters of 20 AI sires previously tested for thyroid activity. Repeatabilities for thyroid traits 2-3 months apart varied, with an average 0.3-0.6 for the different traits. Heritability estimates based on sire components were 0.1-0.4 for the thyroid traits, 0.16 +/- .10 for roughage intake and 0.18 +/- .11 for daily gain. The sire component for feed efficiency (roughage dry matter/kg weight gain) was 0, and genetic correlations between thyroid traits and the production traits in growing heifers were low with large standard errors.     

Effect of Feeding Environment on Performance,Injuries and Behaviour in Growing-finishing Pigs: Group-Based Studies

Ten batches of pigs (608 pigs) were used in this investigation (live weight interval 20-120 kg). Four different feeding systems were tested: one dry feeder, four dry feeders, trough feeding or one wet/dry feeder per pen of 16 pigs, respectively. The one dry feeder treatment led to an increase in skin injuries, a more spread feed intake pattern, an increased variation in carcass meat percentage and an increased variation in daily weight gain (DWG) when the pigs were restrictively fed, compared with observations for the pigs in the four dry feeders treatment. The effect of a reduced number of feeding places was most pronounced when the pigs were fed restrictively. Besides the possible negative financial effects for pig producers, these findings indicated that competition for feed impaired the well-being of the pigs. Pigs fed in a trough had a lower DWG and higher feed conversion ratio than did those fed using four dry feeders, probably due to feed spillage and the different feed intake pattern. Giving pigs the possibility of adding water to the feed increased the daily feed intake when feeding ad libitum, resulting in a higher DWG. It also reduced the negative effects of competition on performance, but not the negative effects on skin injuries.     

Performance,health and behaviour of organic growing-finishing pigs in two different housing systems with or without access to pasture

Abstract

The effects of two housing systems (deep litter and straw-flow), with and without access to pasture during the summer period, were studied in an organic growing-finishing pig herd. Performance, health, skin lesions and daytime pig activity were studied in 29 pens of 16 pigs (total of 464 pigs, in 4 batches). Data from 21 pens were used for comparisons of deep litter and straw-flow and data from 16 pens for comparisons of access/no access to pasture. Pigs in the deep litter system had a lower carcass meat percentage (56.6% vs. 57.3%) and more locomotion problems (4.4% vs. 0%) than pigs in the straw-flow system. No difference in daytime pig activity was detected between the two housing systems. Under moderate temperatures, pigs with access to pasture and fed a commercial organic feed inside the building were not more active during daytime behaviour studies (7.30?h–16.30?h) than pigs without access to pasture. However, at 17 weeks of age the pigs with pasture access spent 21% of their time on pasture, less time inside the pig house (20% vs. 33%) and less time on the outside concrete area (4% vs. 12%) than the pigs without pasture. No difference in performance was detected between pigs with and pigs without access to pasture.     

The effect of feeding system at weaning on performance,health and feeding behaviour of pigs of different sizes

Abstract

Pigs weaned at 31 to 34 days of age (n=280) were mixed into groups of 10 and fed from either feeders (2 feeding places per pen) or troughs (10 feeding places per pen). Analyses on performance, diarrhoea scores and feeding behaviour were made separately on small-, medium- and large-sized pigs, defined as their relative size in the group. During the second week after weaning, feed conversion ratio (FCR) was lower for pigs fed from feeders. Trough feeding resulted in lower total diarrhoea scores for small (p=0.002) and large (p=0.04), and higher scores for medium pigs (p=0.03). Small pigs had lower scores on individual days around the peak on day 6. All three size categories of pigs spent a numerically higher amount of time feeding when fed from a trough. Feeding bouts were longer in pigs fed from a trough (p=0.0002 – 0.05).     

From job training to green jobs: A case study of a young adult employment program centered on environmental restoration in New York City,USA

The demand for a well-trained green-collar labor force will increase as many cities implement sustainability and green infrastructure plans. Additionally, many green jobs training programs are intended to provide pathways out of poverty for low-skilled workers. In this case study, we analyze the experiences of graduates from a New York City, USA green jobs training program targeting 18–24 year olds previously disconnected from the workforce as they transition to full-time employment. Graduates of the program work directly in arboriculture, ecological restoration, landscape design, and horticulture. Despite the well-documented benefits of nature on individual socio-psychological well-being, scant research has investigated the effects of working professionally in urban natural resources management. Our findings reveal the significant challenges facing training program graduates and their supervisors, but also the benefits of urban conservation job training and employment that are potentially transformational for these economically disadvantaged young adults. Green job training and employment present real opportunities for intellectual stimulation and an increased sense of accomplishment, due in part to the uniqueness of environmental work. Individuals reported positive environmental attitudes and behaviors as a result of green jobs training and employment.     

LTP is not a Cysteine Endoprotease Inhibitor in Barley Grains

Several different protease inhibitors have been identified in the mature barley grain, which are proposed to play a defensive role against potential barley pathogens. Cysteine protease inhibitors have been detected in mature grain and in the early stages of germination. The nature of these inhibitors has recently been investigated, and barley lipid transfer protein (LTP1) has been identified as an effective inhibitor of both cysteine and serine endoprotease activity expressed in germinating grain. We show that barley LTP1, in its native state, is not a cysteine protease inhibitor, but in a denatured state becomes a preferred substrate for the barley endoprotease EP-B and, as such, behaves as a competitive inhibitor for poorer substrates of EP-B. The presence of significant amounts of LTP1 in barley malt beer suggests that this very compact protein is highly resistant to proteolytic attack during malting and mashing and its denaturation during wort boiling coincides with inactivation of the malt endoproteases. Analysis of the cleavage products of denatured LTP1, generated by EP-B, provides further evidence for the cleavage site specificity of this barley cysteine endoprotease, where a hydrophobic residue in the P₂position is strongly preferred.     

Gas-phase ionic reactions: dynamics and mechanism of nucleophilic displacements

Nucleophilic displacement reactions (the SN2 reaction) of ions in the gas phase are a prototypical reaction system that allows a study of dynamics, mechanisms, and structure-energy relations. This article reviews aspects of the kinetics (especially the applicability of statistical reaction rate theory), the relation of structure and reactivity, and the effects of small numbers of solvent molecules on the reaction and compares the behavior of the ionic reaction in the gas phase with that in solution.