Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene

Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for the treatment of viral infections. In this study, three siRNA expression plasmids (pS-RepA, pS-RepB and pS-RepC) were generated to target three different coding regions of the Rep protein (Rep) of PCV. These siRNAs were used to inhibit PCV production in a porcine kidney cell line, PK-15 cells. Our results revealed that Rep gene expression was inhibited by pS-RepA, pS-RepB and pS-RepC to different degrees. Moreover, our study also showed that the production of PCV1 and PCV2 was reduced by these siRNAs. pS-RepC, which targets the middle region of Rep gene, proved to be the most efficient siRNA for inhibition of Rep expression and viral production. Taken together, our data suggest that RNAi could be investigated as a potential treatment for PCV infection.     

Hyperthyroidism due to an intrathoracic tumour in a dog with test results suggesting hyperadrenocorticism

The elevated urinary corticoid/creatinine ratios of an 11-year-old Jack Russell terrier with polyuria were suppressible in a high-dose dexamethasone suppression test, which was suggestive of pituitary-dependent hyperadrenocorticism. The absence of physical and routine-laboratory changes compatible with hyperadrenocorticism and the relatively high plasma thyroxine concentration were the impetus for additional studies of thyroid and adrenocortical functions. A high plasma thyroxine concentration (62 nmol/l; 5.0 microg/100 ml) suggested the presence of hyperthyroidism. Radiography, (99m)TcO(4) (-) scintigraphy, ultrasonography, computed tomography and cytology revealed a hyperfunctioning intrathoracic thyroid tumour. In the low-dose dexamethasone suppression test, the plasma cortisol concentration exceeded the reference value of 40 nmol/l (1.4 microg/100 ml) at eight hours after dexamethasone administration (0.01 mg/kg intravenously), a test result compatible with hyperadrenocorticism. In conclusion, this report represents the first case of a dog with an autonomously hyperfunctioning thyroid tumour in the thorax. The elevated urinary corticoid excretion and the positive low-dose dexamethasone suppression test may be explained by alterations in cortisol metabolism, the stress of the hyperthyroid state or both.     

曲拉通和硝酸混合溶液直接提取快速测定草鱼中铅含量

为建立快速测定草鱼中铅含量的方法,通过分析曲拉通和硝酸混合溶液浓度、提取时间、称样定容体积比例等因素的影响,以及基体改进剂的使用效果,确定了草鱼中铅元素提取测定的最佳条件。验证结果显示：采用曲拉通和硝酸混合溶液提取草鱼中铅元素方法的提取率为86.5%～95.6%,加标回收率为91.5%～95.6%,精密度均小于4.0%,检出限和定量限分别为0.011和0.032 mg/kg,满足了国家标准GB 5009.12—2017第一法石墨炉原子吸收光谱法的要求。与国家标准方法比较,本研究建立方法的样品前处理时间从4～12 h缩短至0.2 h内,酸试剂用量从湿法消解的7～12 mL减少至1～2 mL;前处理过程无需高温消解,无需其他前处理设备,避免了样品污染和待测元素损失,实现了降低分析成本以及保护环境和试验人员的目的。结果表明,该方法是一种简单、准确、绿色、快速测定草鱼中铅含量的方法,尤其适合大量样品检测。     

半挂牵引车车架异常断裂原因分析

建立了以板壳单元为基本单元的半挂牵引车车架有限元分析模型,针对半挂牵引车在使用过程中车架异常断裂问题,应用NASTRAN有限元分析软件对车架强度进行了静态及模态分析。有限元分析结果表明,车架断裂现象是由于第4横梁与纵梁连接铆钉处和侧翼板前部的第1个铆钉连接处,应力值已大于或接近材料的最小屈服强度,及在该处出现较大的交变应力而产生的。有限元计算结果与实车车架断裂结果相吻合,证明所采取的建模方法和分析方法是可行的。根据实际工艺要求,给出了该车架结构的改造方法,实际改造结果表明改造方法是非常有效的,断裂区的强度有明显提高。与原结构相比,在弯曲和扭转工况下,最大应力值分别下降38%和57%。     

ANALYTIC SOLUTION OF CYLINDRICAL SHELL ROOF UNDER GENERAL TRANSVERSE LOCAL LOADINGS

This paper uses the method of mixed-type series to derive the analyticsolution of cylindrical shell roof,being simply supported transversely,arbitra-rily restrained longitudinally and subjected to gereral transverse local loads.This paper also presents the analytic expressions of the solutions for somekinds of local load.Some examples of this kind of shell roof subjected to ainlined single force near the middle of it with free lorgitudinal edges aregiven.The computation shows that the convergence of the solution is woll     

试谈现代企业成本管理的变革

现代企业生产经营是以提高经济效益为目标的。要提高经济效益,必须加强企业的成本管理。因此,在现代企业制度中强调成本管理十分重要。长期以来,在高度集中的计划经济体制下,国家通过指令性计划直接对企业进行调控。所以,企业的成本管理观念比较淡薄,即使讲成本管理,也主要是从降低消耗、节约费用考虑,侧重于对产品成本的核算,不注重所消耗成本能得到的市场价值,不在提高产品或服务的质量和功能上做文章。在社会主义市场经济体制下,企业所面临的外部和内部环境都发生了根本性的变化,传统的成本管理越来越不能适应新形势的需要。企业为了能在…     

动态油膜-裂纹转子系统非线性行为研究

利用一种非线性非稳态油膜力模型，以轴承-裂纹转子为研究对象，用数值积分结合Poincare映射和谱分析，研究了系统的非线性动力学行为。结果表明，在特定参数组合下系统中存在混沌、概周期运动等复杂的动力学行为，所得结果为同类型转子的设计及故障检测与诊断提供了一定的依据。     

变螺距诱导轮流场的数值模拟

在泵叶轮进口前置变螺距诱导轮能有效地提高泵机组的抗汽蚀性能。基于CFD技术,采用Fluent软件对变螺距诱导轮进行几何建模、选定边界条件等,数值模拟计算其内部流场。结合变螺距诱导轮的实例,给出了变螺距诱导轮内部速度与压力的分布情况以及粒子流动迹线图,得到了其内部流场的主要特征。     

河北省农药可追溯管理的调查思考

本文对河北省农药可追溯管理现状进行了介绍和分析,介绍了河北省农药可追溯管理的形式,分析了目前存在的问题,并对下一步如何深入做好农药的可追溯管理提出了建议。