牛副结核检疫中的非特异性干扰

给犊牛接种各种分枝杆菌，然后以副结核ＰＰＤ进行变态反应试验，观察各分枝杆菌感染对副结核变态反应的干扰；从各种分枝杆菌感染牛群采取血清进行副结核ＥＬＩＳＡ检测，以观察各分枝杆菌血清对牛副结核ＥＬＩＳＡ的干扰。结果：多种分枝杆菌，特别是鸟胞内分枝杆菌、瘰疬分枝杆菌的感染可干扰副结核变态反应；部分结核牛血清干扰副结核ＥＬＩＳＡ的检测     

LFA—3和IL—2对机体免疫增强作用

以绵羊红细胞膜提取的ＬＦＡ－３和猪外周血单个核细胞所产生的ＩＬ－２，同时或分别与新城疫Ⅳ系疫苗胸肌注射２２日龄雏鸡，测定鸡体ＨＩ抗体效阶及Ｅｔｇ花环形成率的变化，结果表明，ＬＦＡ－３组抗体效价与对照组差异极显著，ＩＬ－２也可显著提高ＩＶ系苗免疫后ＨＩ抗体效价，且两者的作用具有剂量依赖性。     

Equine chronic diarrhea: Traditional Chinese veterinary medicine review

Chronic diarrhea is a common problem in equine practice. Herbal medicine has been used for the treatment of gastrointestinal disease in horses for at least one thousand years. In present-day equine practice in China, the most common approach to treat chronic diarrhea is herbal medicine, or a combination of herbal medicine and acupuncture. Three types of chronic diarrhea in horses will be described in detail in term of symptoms, diagnosis and herbal treatments in this paper. Nineteen herbal formulas for treatment of different types of diarrhea will be introduced. Clinical results of each herbal formula will be evaluated. Acupuncture treatment of this problem will also be included.     

THE SCINTIGRAPHIC EVALUATION OF SOLID PHASE GASTRIC EMPTYING IN NORMAL CATS

The gastric emptying half-time of solid food in normal cats was determined using a scintigraphic technique. ^99mTc-sulfur colloid was applied to dry food. Sequential images were acquired post prandial, and the gastric emptying half-time was determined for several different acquisition and processing parameters. Good correlation was found between different acquisition and processing methods, some of which made use of edge detection applications, while others used different views, or number of views from which the gastric emptying half-time was calculated. The mean ± standard deviation, (range) and median of the gastric emptying half-time determined using the geometric mean of total counts in hand-drawn regions of interest from the lateral and ventral images were 2.47 × 0.71, (1.42–3.61) and 2.37 hours respectably.     

A COMPARISON OF MANUAL AND AUTOMATIC ULTRASOUND-GUIDED BIOPSY TECHNIQUES

A manual, ultrasound-guided biopsy technique was compared to a new automatic method using a new biopsy device. The liver and the left kidney of 10 dogs were biopsied using the two methods. The specimens from the biopsies were compared for size and quality. Results demonstrate that larger and higher quality samples were obtained with the new automatic method.     

阿鲁科尔沁旗紫花苜蓿需水规律与灌溉定额

为了给阿鲁科尔沁旗紫花苜蓿(Medicago sativa)灌溉提供科学依据,利用1984-2013年30年气象数据,采用联合国粮农组织推荐的彭曼-蒙特斯公式法,研究了阿鲁科尔沁旗紫花苜蓿需水规律和灌溉定额。结果表明,阿鲁科尔沁旗紫花苜蓿第1茬、第2茬、第3茬、第4茬、生长季、非生长季和全年需水量分别为221、187、169、179、755、70和825mm,需水强度分别为4.3、4.7、4.1、2.5、3.7、0.4和2.3 mm·d~(-1),灌溉需水量分别为194、118、66、131、508、56和564mm,灌溉定额分别为228、139、78、154、598、66和664 mm。     

Serum α-globulin fraction in horses is related to changes in the acute phase proteins

Serum electrophoresis is a useful technique to diagnose some diseases characterized by quantitative and qualitative changes in the serum proteins, such as the acute phase proteins. An increased synthesis of acute phase proteins has been associated with acute infection and inflammation. We wanted to study whether gastrointestinal diseases and disorders of the locomotor system in horses produce enough inflammatory reaction so the acute phase proteins can be detected by electrophoresis in serum. Serum total proteins were calculated, and the serum electrophoresis from 63 horses was performed. The horses were classified in 3 groups: Group I (19 healthy horses), Group II (20 horses suffering from inflammatory disorders of the gastrointestinal tract), and Group III (24 horses with inflammatory disorders of the locomotor system). Results indicated an increase of the acute phase proteins only in patent inflammatory disorders that showed a rapid progression. A global increase in those proteins was observed only for α₁-globulins from Group II.     

Effect of embryo age on the viability of equine embryos after cooled storage using two transport systems

The aim of this study was to compare the viability of 7- and 8-day-old equine embryos cooled and stored for 6 or 24 hours in two different transport systems. Embryos (n = 97) were recovered on day 7 or 8 and assigned to 10 groups (n = 10/group). Embryos within the same age group (D7 or D8) were evaluated immediately after collection (Group-0h) or after storage in an Equitainer at 5°C for 24 hours in 5 ml Emcare Holding Solution (EHS) (Group-E-24h) or 5 ml Ham's F10 (Group-H-24h) or in a refrigerator at 5°C in 500 ml Emcare Flushing Solution (EFS) for 6 hours (Group-B-6h) or 24 hours (Group-B-24h). After collection or storage, embryos were incubated in 1 μg/ml DAPI to determine the percentage of dead cells per embryo (DAPI positive, fluorescent cells). Subsequently, embryos were fixed in 4% paraformaldehyde and re-stained with DAPI to determine the total number of cells. The percentage of dead cells in group-0h and B-6h was similar and significantly lower than for embryos stored for 24 hours in groups B-24h, E-24h, and H-24h. The percentage of dead cells was similar for embryos stored in an Equitainer (groups E-24h and H-24h) and was significantly higher for embryos stored 24 hours in EFS (Group B-24h). Within each storage system (0h, B-6h, B-24h, E-24h, and H-24h) no significant difference in the percentage of dead cells was observed between 7- and 8-day-old embryos. Storage in 500 ml EFS at 5°C for 6 hours resulted in embryos of better quality than after the traditional 24-hour storage in an Equitainer, suggesting that this simplified system offers a good alternative for short-term storage and transport.