Cardioprotective effect of 'Marutham' a polyherbal formulation on isoproterenol induced myocardial infarction in Wistar rats

Myocardial infarction is the number one killer disease in many parts of the world. The cardioprotective effect of Marutham, a polyherbal formulation on serum and heart tissue lipids, serum lipoproteins and heart membrane bound enzymes in isoproterenol induced myocardial infarction was studied in Wistar rats. Pretreatment with Marutham at different doses of 30, 60 and 90 mg kg(-1) to isoproterenol treated rats significantly prevented the altered lipid profile and membrane bound enzymes to near normal status. The results of our study showed the cardioprotective potential of Marutham on isoproterenol induced myocardial infarction in rats.     

Carbon input, loss and storage in sub-tropical Indian Inceptisol under multi-ratooning sugarcane

Changes in residue management and incorporation of organic manures may help in carbon sequestration, restoring soil organic carbon (SOC) and sustaining the productivity of land under a cropping system. An experiment of multi-ratooning sugarcane (Saccharum officinarum L.) was initiated in 2003 in Inceptisols of Indian subtropics, to assess the effect of different organic manures and chemical fertilizer, on the crop productivity and soil quality. The annual sugarcane shoot biomass production in organic manure treatments was at par with the chemically fertilized treatment. Gross input of carbon (GIC) by the sugarcane crop was estimated to be 11.7–12.4 t ha⁻¹ y⁻¹ in different organic manure treatments compared to 8.4 and 5.0 t ha⁻¹ y⁻¹ in NPK and control treatments, respectively. The respiratory loss of C (RLC) increased linearly with increasing input of C in soil and it ranged from 3.3 to 4.1 t ha⁻¹ y⁻¹ in different treatments with maximum in FYM and minimum in control treatment. The sugarcane biomass added in the soil humified at a rate constant of 0.38 in sub-tropical conditions and an addition of 3.9 t C ha⁻¹ y⁻¹ is required to maintain SOC in equilibrium. After 5 years of sugarcane cropping (one plant + four ratoons) an increase of 2.3–17.1 t ha⁻¹ in SOC over initial content was recorded with different treatments. Results in coming years from this long-term experiment shall add to the present calculated relationships between carbon addition and storage in sugarcane multi-ratooning crop production system under sub-tropical condition of India.     

Isolation,Purification, and Biochemical Characterization of Trypsin from Indian Mackerel (Rastralliger kanagurta)

Trypsin from viscera of Indian mackerel (Rastralliger kanagurta) was purified by ammonium sulphate precipitation and chromatographic techniques such as size exclusion, ion exchange, and affinity chromatography, with a 14.4-fold increase in specific activity and 18.7% recovery. The molecular weight of the trypsin was estimated to be approximately 26 kDa using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Purified trypsin showed amidase-specific activity which was determined using benzoyl-dl-arginine-p-nitroanilide (BAPNA). The optimum pH and temperature for isolated trypsin activity were 9.0 and 50°C, respectively. The purified trypsin was strongly inhibited by soybean trypsin inhibitor (SBTI) and N-p-tosyl-1-lysine chloromethyl ketone (TLCK). Purified trypsin showed almost 40% recovery at high NaCl concentration (30%). The N-terminal amino acid sequence of the first 10 amino acids of purified trypsin was IVGGYESQPH. The Michaelis-Menten constant (K_m) and catalytic constant (K_cat) of purified trypsin were 0.430 mM and 0.77 s^?1, respectively, determined using BAPNA as a substrate. Purified trypsin showed digestion of casein similar to bovine trypsin by the fluorometric method.     

Comparison of Carbon Stocks Between Mixed and Pine-Dominated Forest Stands Within the Gwalinidaha Community Forest in Lalitpur District, Nepal

Forests play an important role in the global carbon cycle as both a source and sink of carbon. The carbon stock in a forest is affected by climate, tree species and forest management. The community forestry program of Nepal has been successful in reviving degraded forest patches in the Mid-hills but there is a lack of information whether mixed or pine plantations store more carbon. This study estimated and compared carbon stocks in mixed and pine-dominated forest stands within the Gwalinidaha Community Forest of Lalitpur District, Central Nepal. Carbon components considered include tree biomass carbon, root biomass carbon, litter biomass carbon and soil organic carbon. Total carbon stock of the forest is estimated to be 2,250.24 tons with average carbon stock of 166.68 tons/ha. Total carbon stock per hectare was found to be higher in the pine-dominated forest as compared to mixed forest due to the larger tree biomass although the litter carbon and soil organic carbon estimates are higher in the latter. The Community Forestry of Nepal has a huge potential for carbon storage and the pine-dominated forest has a greater carbon stock than mixed forest.     

Helical structures of ESCRT-III are disassembled by VPS4

During intracellular membrane trafficking and remodeling, protein complexes known as the ESCRTs (endosomal sorting complexes required for transport) interact with membranes and are required for budding processes directed away from the cytosol, including the budding of intralumenal vesicles to form multivesicular bodies; for the budding of some enveloped viruses; and for daughter cell scission in cytokinesis. We found that the ESCRT-III proteins CHMP2A and CHMP3 (charged multivesicular body proteins 2A and 3) could assemble in vitro into helical tubular structures that expose their membrane interaction sites on the outside of the tubule, whereas the AAA-type adenosine triphosphatase VPS4 could bind on the inside of the tubule and disassemble the tubes upon adenosine triphosphate hydrolysis. CHMP2A and CHMP3 copolymerized in solution, and their membrane targeting was cooperatively enhanced on planar lipid bilayers. Such helical CHMP structures could thus assemble within the neck of an inwardly budding vesicle, catalyzing late steps in budding under the control of VPS4.     

Population structure,gene flow and genetic diversity analyses based on agro-morphological traits and microsatellite markers within cultivated and wild germplasms of okra [Abelmoschus esculentus (L.) Moench.]

Genetic Resources and Crop Evolution - Okra [Abelmoschus esculentus (L.) Monech.] is an annual herb of the family Malvaceae and has tremendous nutritional and industrial potential. However, the...     

What Can Be Gleaned From News Coverage to Improve Science Reporting and Enhance Public Literacy About Agricultural Biotechnology in Ghana?

A content analysis of print and online sources was conducted to assess the performance of the Ghanaian news media in reporting about agricultural biotechnology. The findings show the dominant presence of food security and food safety issues in the coverage of genetically modified organisms that was overwhelmingly negative toward genetic engineering and its agricultural products. Members of the food industry and government officials (mainly politicians) were the most commonly cited information sources. A qualitative examination of news discourse suggests ways to strengthen the effectiveness of communicating agricultural information that will enhance public literacy and foster intelligent decision making about this controversial topic.     

苹果属无融合生殖相关基因SERK1遗传转化的研究

本研究以p BI121为植物表达载体,在已构建的无融合生殖基因表达载体p BI121-Mh SERK1和p BI121-Mhd SERK1的基础上,利用根癌农杆菌介导法,将苹果属无融合生殖相关基因SERK1转入受体植株烟草中。研究结果表明:烟草叶片浸染后,经共培养和选择培养形成再生芽,分别获得7株p BI121-Mh SERK1和6株p BI121-Mhd SERK1转烟草抗性植株,PCR检测证明已成功获得Mh SERK1和Mhd SERK1烟草转化株系。本研究可为下一步无融合生殖相关基因功能验证研究奠定基础。