Phytoestrogen content of beverages, nuts, seeds, and oils

Phytoestrogens are secondary plant metabolites that have received increasing attention for their bioactivity, in particular due to their structural and functional similarity to 17beta-estradiol. Although urinary and plasma phytoestrogens can be used as biomarkers for dietary intake, this is often not possible in large epidemiological studies or in the assessment of general exposure in free-living individuals. Accurate information about dietary phytoestrogens is therefore important, but there are very limited data concerning food contents. In this study was analyzed a comprehensive selection of tea, coffee, alcoholic beverages, nuts, seeds, and oils for their phytoestrogen content using a newly developed sensitive method based on LC-MS incorporating (13)C 3-labeled standards. Phytoestrogens were detected in all foods analyzed, although the contents in gin and bitter (beer) were below the limit of quantification (1.5 microg/100 g). Lignans were the main type of phytoestrogens detected. Tea and coffee contained up to 20 microg/100 g phytoestrogens and beer (except bitter) contained up to 71 microg/100 g, mainly lignans. As these beverages are commonly consumed, they are a main source of dietary lignans. The results published here will contribute to databases of dietary phytoestrogen content and allow a more accurate determination of phytoestrogen exposure in free-living individuals.     

Differential response of ectomycorrhizal and saprotrophic fungal mycelium from coniferous forest soils to selected monoterpenes

The mycelia of saprotrophic (SP) and ectomycorrhizal (ECM) fungi occur throughout the upper soil horizons in coniferous forests and could therefore be exposed to high concentrations of monoterpenes occurring in the needle litter of some tree species.Monoterpenes are mycotoxic and could potentially affect fungi that are exposed to them in the litter layers. In order to investigate whether monoterpenes typical of coniferous litters could influence fungal communities, we analysed the monoterpene content of freshly fallen needles of Pinus sylvestris, Picea abies and Picea sitchensis. The most abundant monoterpenes were found to be α-pinene, β-pinene and 3-carene. We evaluated the effects of these three monoterpene vapours on the biomass production of 23 SP isolates and 16 ECM isolates. Overall, 75% of ECM isolates and 26% of SP isolates were significantly inhibited by at least one of the monoterpene treatments and both intra- and inter-specific variations in response were observed.Monoterpene concentrations are highest in surface litters. The differential effects on fungal taxa may influence the spatial and temporal distribution of fungal community composition, indirectly affecting decomposition and nutrient cycling, the fundamental ecosystem processes in which fungi have a key role in coniferous forest soils.     

Mineralogy and petrology of comet 81P/Wild 2 nucleus samples

The bulk of the comet 81P/Wild 2 (hereafter Wild 2) samples returned to Earth by the Stardust spacecraft appear to be weakly constructed mixtures of nanometer-scale grains, with occasional much larger (over 1 micrometer) ferromagnesian silicates, Fe-Ni sulfides, Fe-Ni metal, and accessory phases. The very wide range of olivine and low-Ca pyroxene compositions in comet Wild 2 requires a wide range of formation conditions, probably reflecting very different formation locations in the protoplanetary disk. The restricted compositional ranges of Fe-Ni sulfides, the wide range for silicates, and the absence of hydrous phases indicate that comet Wild 2 experienced little or no aqueous alteration. Less abundant Wild 2 materials include a refractory particle, whose presence appears to require radial transport in the early protoplanetary disk.     

Electroencephalographic assessment of oral meloxicam,topical anaesthetic cream and cautery iron for mitigating acute pain in pigs (Sus scrofa) undergoing tail docking

Objective

To evaluate the efficacy of oral meloxicam, topical anaesthetic cream and cautery iron in mitigating acute nociceptive responses of pigs to tail docking.

Quantification of accelerated soil erosion using the environmental tracer caesium-137

The principal objective of this investigation was to quantify erosion rates for five agricultural fields in three separate study areas in Saskatchewan. The radionuclide tracer caesium-137 (¹³⁷Cs) was used to quantify net erosion and net deposition within the landscape over a 30-year period. Uneroded (native) sites were used to establish the mean background level of ¹³⁷Cs in each of the study areas. The assumption being that agricultural sites with ¹³⁷Cs areal activities greater than the native site were subject to deposition, and sites with ¹³⁷Cs less than the native control site were subject to erosion. A linear proportionality model was used to convert the loss or gain of ¹³⁷Cs to net soil erosion or deposition. Results have indicated that accelerated (anthropogenic) erosion has been commonplace on arable land in Saskatchewan, even on near-level fields (< 1.3 degrees). The net integrated sediment output from the five agricultural fields ranged from — 0.6 t th⁻¹ y⁻¹ to — 6.8 t ha⁻¹ y⁻¹ (where negative values represent erosion). What is more alarming is that between 40 and 75 per cent of all sites sampled within individual fields had erosion rates in excess of the generally accepted rate of soil formation (1.0 t ha⁻¹ y⁻¹). Also, in one highly eroded field (Crystal Springs medium sloping site) 65 per cent of the sites sampled exceeded the upper tolerable erosion rate of 11.0 t ha⁻¹ y⁻¹. These results indicate significant degradation of the non-renewable soil resource has occurred over the past 30 years and is still presently active. Land degradation by accelerated erosion would result in reductions in effective rooting depth, soil moisture holding capacity, essential nutrient stores, and would adversely effect the physical structure of the topsoil. The major reason for accelerated erosion on arable land in Saskatchewan is the practice of summer fallowing, where the field is left in a ‘bare’ state and repeatedly tilled every second or third year. During a fallow period, or prior to crop emergence during a cropping year, fields are subject to wind and water erosion. On near-level fields wind would be the dominant transport agent, while on sloping fields inter-rill and rill erosion would be the primary forces of erosion. It is suggested that the appropriate conservation farming response would be to increase application of surface mulches and possibly to decrease the frequency of summer fallowing. Without such efforts long-term sustainable agricultural production in the Prairies of Canada is considered to be a tenuous land use practice.     

Hydrophysical degradation associated with hiking‐trail use: a case study of Hawai'iloa Ridge Trail,O'ahu,Hawai'i

Recreational activities can have significant impacts on geomorphic and hydrologic processes in drainage basins, often out of proportion to their areal extent. With increased stress on hiking trails nationwide, there is a need to characterize the impacts of human trampling on soil properties. We examine an 810 m segment of Hawai'iloa Ridge Trail (O'ahu, Hawai'i, USA). Soil compaction and surface erosion on moderate to steeply sloping sites have degraded the trail environment. Bulk density, penetration resistance, and vane shear strength were significantly higher on the trail than in adjacent undisturbed areas, with median differences ranging from 29 to 120 per cent. With compaction and exposure of subsoil on the trail, void ratio, air‐filled porosity, saturated hydraulic conductivity, effective and preferential porosity were significantly lower, with relative change values ranging from 23–93 per cent. No significant changes were noted in meso‐ or micro‐porosity, but macropores with a radius of >110 μm decreased significantly by 58 per cent for on‐trail locations. This comprehensive dataset indicates that hiking stress is deleterious to the soil–hydrologic system. Data point to a trail system that would be dominated by Hortonian overland flow and this was supported by field evidence during a storm event. Increased runoff has incised rills on some trail segments and there is evidence that run‐on to adjacent side slopes has lead to accelerated erosion. Management on most trails in Hawai'i, including the one studied, is limited, but from our data it is apparent that on‐trail sites directly influenced by an overhanging canopy of rapidly growing (aggressive) exotics were least impacted due to increased organic contributions to the surface and root network development. These data will allow land managers to more effectively address the potential geomorphic and hydrologic impacts of recreational land use. Copyright © 2001 John Wiley & Sons, Ltd.