Pitfalls in immunofluorescence testing in canine dermatology

Indirect immunofluorescence testing for pemphigus-like antibodies was performed on 100 dogs: 50 dogs with various nonpemphigus dermatologic diseases, 25 dogs with various nondermatologic diseases, and 25 normal dogs. One dog (generalized demodicosis) was positive for pemphigus-like antibodies at a titer of 1:10. It was concluded that canine pemphigus-like antibodies are a potential source of misinterpretation and misdiagnosis in indirect immunofluorescence testing. Direct immunofluorescence testing for IgG, IgA, IgM, and C3 was performed on the footpads of 11 normal dogs. Granular deposition of IgM at the basement membrane zone was demonstrated in 5 of the 11 dogs. It was concluded that direct immunofluorescence testing of canine footpads using only polyvalent immunoglobulin antisera or anti-IgM antisera may lead to misinterpretation and misdiagnosis in up to 45% of all dogs tested.     

Pancreatic endocrinopathies in ferrets.

Pancreatic endocrinopathies, especially insulinomas, are a common finding in ferrets. Surgical resection remains the treatment of choice for insulinomas because it can provide longer disease-free intervals and survival times. Because of the high rate of metastasis, owners should be advised that treatment is rarely curative and is aimed at controlling the clinical signs of hypoglycemia by stopping or slowing the progression of the insulinoma. Although less common, diabetes mellitus can occur in ferrets, but is often a postsurgical sequela to insulinoma resection. Current advancements in diabetes management can be applied to ferrets with diabetes mellitus.     

Quantitative Evaluation of Equine Deep Digital Flexor Tendon Morphology using Magnetic Resonance Imaging

Injury of the distal aspect of the deep digital flexor tendon (DDFT) is a recognized cause of lameness, but diagnosis is difficult. This study aimed to improve understanding of DDFT morphology and pathology using retrospective evaluation of magnetic resonance (MR) images. We hypothesized that: (1) The distal aspect of the DDFT in normal horses would have a repeatable proximal/distal pattern and symmetry between limbs and between lobes; (2) DDFT dimensions would be related to bodyweight, navicular bone dimensions and hoof size; (3) this symmetry and pattern would be lost in DDFT injury; and (4) DDFT size would increase with injury. MR images of 64 live horse limbs, 26 with no identified DDFT lesion and 38 with identified DDFT abnormalities, and 19 normal cadaver limbs were analyzed. Using standardized transverse images, measurements of DDFT cross-sectional area, medial-lateral (ML) width and dorsal-palmar depth were obtained at six preselected sites. A uniform distal to proximal shape pattern was identified in all horses. The flattened crescent shape at the insertion changed to a deeper bilobed shape more proximally, with the mid-navicular area having the greatest cross-sectional area. Strong ML (P < 0.0006) and left/right symmetry (P < 0.02) were observed. In addition, there was a strong association between DDFT cross-sectional area and horse weight (P = 0.005) and between DDFT and navicular bone ML width (P = 0.004). Symmetry between sides or between lobes was lost at sites with a unilateral lesion and correlation between horse weight and DDFT cross-sectional area was lost in the presence of lesions. DDFTs with core lesions had a consistent increase in cross-sectional area overall, but other lesion types had no significant increase in size. The shape and symmetry seen in normal tendons could be related to the mechanical demands placed upon individual lobes. The limited increase in cross-sectional area with injury may be explained by the restrictive structures of the hoof, possibly explaining the ongoing pain seen in such lesions.     

Barley and Wheat Share the Same Gene Controlling the Short Basic Vegetative Period

Basic vegetative period（BVP） is an important trait for determining flowering time and adaptation to variable environments.A short BVP barley mutant is about 30 d shorter than its wild type.Genetic analysis using 557 F 2 individuals revealed that the short BVP is governed by a single recessive gene（BVP-1） and was further validated in 2 090 F 3 individuals.The BVP-1 gene was first mapped to barley chromosome 1H using SSR markers.Comparative genomic analysis demonstrated that the chromosome region of BVP-1 is syntenic to rice chromosome 5 and Brachypodium chromosome 2.Barley ESTs/genes were identified after comparison with candidate genes in rice and Brachypodium;seven new gene-specific markers were developed and mapped in the mapping populations.The BVP-1 gene co-segregated with the Mot1 and Ftsh4 genes and was flanked by the gene-specific markers AK252360（0.2 cM） and CA608558（0.5 cM）.Further analysis demonstrated that barley and wheat share the same short BVP gene controlling early flowering.     

A framework for integrating the terrestrial carbon stock of estates in institutional carbon management plans

Many institutions have substantial landholdings, but few consider soil carbon preservation and augmentation in their carbon management plans. A methodical framework was developed to analyse terrestrial carbon stocks (soil and tree biomass) for credible carbon offsetting strategies in institutional land. This approach was demonstrated at two farms (805 hectares) managed by Newcastle University. Soil carbon for three depths (0–30 cm, 30–60 cm and 60–90 cm) and above-ground tree biomass were quantified. These data provided a terrestrial carbon baseline to evaluate future land management options and effects. Historical land-use records enabled the following comparisons: (1) agricultural land vs. woodland; (2) arable land vs. permanent grassland; (3) organic vs. conventional farming; (4) coniferous vs. broadleaved woodland; and (5) recent vs. long-established woodland. Carbon storage (kg/m²) varied with land usage and woodland type and age, but only agricultural land vs. woodland, and for agriculture, arable land vs. permanent grassland, significantly affected the 0–90 cm soil carbon. At the university-managed farms, current terrestrial carbon stocks were 103,620 tonnes in total (98,050 tonnes from the 0–90 cm soil and 5,569 tonnes from tree biomass). These terrestrial carbon stocks were equivalent to sixteen years of the current carbon emissions of Newcastle University (6,406 tonnes CO₂ equivalents-C per year). Using strategies for alternative land management, Newcastle University could over 40 years offset up to 3,221 tonnes of carbon per year, or 50% of its carbon emissions at the current rate. The methodological framework developed in this study will enable institutions having large landholdings to rationally consider their estates in future soil carbon management schemes.