Exothermic heat released during catalytic polymerization of basswood-methyl methacrylate composites

Summary Oven dried basswood was vacuum impregnated with methyl methacrylate monomer containing various amounts of free radical catalyst and trifunctional crosslinking agent. The wood-monomer composite was polymerized at 60°C in an air oven and the rate of heat generated was followed by means of a thermocouple placed in the center of the sample.Increasing the concentration of Vazo free radical catalyst in the basswood-MMA impregnated samples reduces the time to the exothermic peak and increases the exothermic peak temperature. High Vazo concentrations also decraase the percentage conversion of monomer to polymer.Trimethyl propane trimethacrylate (TMPTMA) crosslinker reduces the time to the exothermic peak and dramatically increases the exothermic peak temperature. The use of a crosslinker rapidly increases the initial viscosity of the system, and therefore, the autoacceleration of the polymerization is more pronounced.Our thanks are due to DuPont Company and Sartomer Resins for supplying the monomers and catalysts. Research funds were provided by Organized Research Budget of the College of Forestry.     

Functional secretion of a type 1 antifreeze protein analogue by optimization of promoter, signal peptide, prosequence, and terminator in Lactococcus lactis

Lactococcus lactis is a food-grade microorganism of major commercial importance. Antifreeze protein is a potent cryogenic protection agent for the cryopreservation of food and pharmaceutical materials. In this study, extracellular expression of a novel recombinant type I antifreeze protein analogue (rAFP) in L. lactis was optimized. An efficient SlpA promoter (P SlpA) was fused to various signal peptides (SPs) and propeptide sequences to examine the extracellular expression levels of rAFP. An efficient signal peptide, SP sacB, fused to prosequence AE, enabled higher extracellular rAFP production; use of the SlpA terminator (Ter SlpA) was a further improvement. The extracellularly expressed rAFP successfully inhibited ice recrystallization and is thus potentially applicable for cryogenic preservation.     

Comparative analysis of Australian and Canadian barleys for seed dormancy and malting quality

The association between high malting quality and pre-harvest sprouting (PHS) susceptibility is a key challenge when developing new malting barley varieties. A new malting barley variety Baudin has successfully combined high malting quality and PHS tolerance. A doubled haploid population was developed for mapping PHS tolerance and seed dormancy from a cross of Baudin?×?AC Metcalfe using 233 molecular markers. Three QTLs were mapped for seed dormancy based on the standard germination test at 24, 48 and 72?h. One major QTL was mapped to the long arm of chromosome 5H controlling seed dormancy and PHS tolerance from Baudin. Two other minor QTLs were identified from Baudin on chromosomes 3 and 7H. QTL/QTL interaction was detected for seed dormancy between chromosomes 3 and 5H. The PHS tolerance allele of the 5H QTL from Baudin contributes to higher malt yield without significant impact on diastatic power, beta-glucan content and wort viscosity. QTL from Baudin provide new sources to integrate PHS tolerance and high malting quality.     

Evaluation of Two Reagent Strips and Three Reflectance Meters For Rapid Determination of Blood Glucose Concentrations

We evaluated three reflectance meters (Accu-Chek II, Glucometer II, and Glucoscan 2000) and two reagent strips (Chemstrip bG and Glucostix) for accuracy and precision in determining blood glucose concentrations in the dog. To evaluate accuracy, we compared results of blood glucose determinations performed on 95 samples using the various strips and meters vs. the glucose concentrations obtained using the glucose-oxidase method on a Beckman Glucose Analyzer. Accuracy was evaluated statistically using least squares regression analysis. To evaluate precision, samples in various ranges of blood glucose concentration were tested repeatedly (20 times within a 1-hour period) on the same reflectance meter. Coefficient of variation (CV) was determined to evaluate reproducibility of results. Overall, there were significant correlations (P less than 0.001) between the laboratory glucose values and the blood glucose concentrations obtained with Chemstrip bG (r = 0.976), Glucostix (r = 0.904), Accu-Chek II (r = 0.986), Glucometer II (r = 0.911) and Glucoscan 2000 (r = 0.944). In the precision study, all three meters had excellent CVs in the normal range (3.6% to 4.9%). However, Accu-Chek II was found to be more precise in the hypoglycemic and hyperglycemic ranges (3.6% and 2.6%, respectively) than either Glucometer II (8.8% and 5.4%) or Glucoscan 2000 (7.8% and 8.2%). The results of this study indicate that all of the meters and reagent strips tested are highly accurate in determining blood glucose concentrations in the dog. However, both in terms of accuracy and reproducibility of results, Accu-Chek II and Chemstrip bG, gave the highest correlation coefficients and, as such, are probably of the greatest clinical value.     

The effect of free amino acid levels in the blood and milk of cows on protein levels in milk

In the cows of the whole group under study, a significant negative relation (r = = -0.4797) was found between the histidine level in arterial blood and protein content in milk; further, there was a significant positive relation (r = 0.4624) between alanine level in milk and protein content in milk, and a significant negative relation (r = -0.5028) for the level of threonine. The group of cows with a higher milk output showed a significant negative relation between methionine level in milk and protein content in milk (r = -0.7482) and a highly significant negative relation between threonine level and protein content in milk (r = -0.8410). The negative relations of some amino acids to the content of protein in milk suggest that there is a poor supply of these amino acids for the production of protein in milk.     

Isolation of Feline Herpesvirus 1 from the Trigeminal Ganglia of Acutely and Chronically Infected Cats

Feline herpesvirus 1 (FHV-1) is one of the most common viral infections of domestic cats worldwide, estimated to cause 50% of all respiratory infections in this species. Feline herpesvirus 1 is also an important ocular pathogen of cats, causing conjunctivitis, epithelial and stromal keratitis, symblepharon formation, keratocon-junctivitis sicca, and corneal sequestration. Despite the importance of this viral disease, major questions remain unanswered concerning the pathogenesis of its most important manifestation, the recrudescent infection. Although the taxonomic classification of FHV-1 as an alpha herpesvirus implies the ability of FHV-1 to establish neural latency, attempts at recovering the virus from the trigeminal ganglia of latently infected cats have typically yielded negative results. This failure has stimulated speculation that neural tissue is not an important site for latent FHV-1. However, in the most successful of such studies, FHV-1 was isolated from the trigeminal ganglia of 3 of 17 cats using an explant technique. In the present study, we describe the successful isolation of FHV-1 from the trigeminal ganglia of cats using a similar tissue culture method.