美洲黑杨基因资源收存及其遗传评价的研究

从１７个国家引进３３１个黑杨派无性系（其中５２个美洲黑杨无性系）,在山东省长清县营建我国第一个黑杨派无性系基因库。连续１０年对基因库内美洲黑杨无性系进行了多性状系统研究,结果表明各无性系间在物候期、生长、生根、抗寒和抗病虫等方面均存在着显著的差异,遗传变异丰富;系间的主要性状与无性系起源纬度及各性状间存在一定相关性。主要材料性状变异的研究结果表明,３８个８年生美洲黑杨无性系在木材基本密度、纤维长度无性系间变异达到极显著水平;木材密度和纤维长度与树高和胸径呈一定相关性;木材密度和纤维长度这两个性状在遗传上相互独立,受不同遗传机制控制。利用分子遗传标记ＲＡＰＤ技术研究库内美洲黑杨无性系ＤＮＡ多态性,结果表明本库美洲黑杨ＤＮＡ多态率为８６％,再次证明本库美洲黑杨遗传多样性高,可为我国杨树改良提供丰富的育种材料。     

干法灰化—石墨炉原子吸收法测定面制食品中铝含量

建立了面制食品中铝残留量的石墨炉原子吸收测定方法。通过干法灰化样品,筛选最佳石墨炉原子吸收分析条件,并对面制食品中铝进行测定。结果显示,该法在质量浓度20～50μg/L内呈线性关系,相关系数R=0.9998,回收率96.4%～98.5%,RSD为1.46%～3.32%;定量限下限7.35μg/L,最低检出质量浓度3.50μg/L。该方法结果符合要求,操作简便、快速、准确、干扰小、检出限低,适用于面制食品中铝含量的测定。     

Adhesion of Escherichia coli K88ab fimbriae to porcine enterocytes: effect on enzymatic activities of the brush border, and cyclic AMP and GMP levels

We have studied the cellular alterations, after in vitro adherence, of purified K88ab fimbriae to membranes of porcine enterocytes. Effects on enzymatic activities as disaccharydases and alkaline phosphatase show low changes. While cAMP levels were decreased (44%), guanylyl cyclase was increased (up to 200%), and levels of cGMP were in consequence significantly affected. This study support the role of cyclic GMP as intracellular mediator for adherence, and suggest their implication in disease, affecting a membrane-mediated mechanisms for guanylate cyclase activation, that is unique in the intestine.     

马立克氏病疫苗胚胎免疫鸡红细胞免疫功能的研究

作者检测了胚胎免疫鸡（孵化至18日龄时鸡胚接种马立克氏病二价疫苗）及其相应1日龄免疫鸡和未免疫鸡的红细胞CR1花环率和红细胞IC花环率以观察其外周血液红细胞免疫功能。结果表明,随着试验鸡日龄的增长,其外周血液中红细胞CR1活性呈现一定的递减性变化,但胚胎免疫鸡红细胞CR1活性的降低幅度远低于其它两组鸡。胚免鸡出壳后7日龄进行vMDV攻毒,结果也显示胚胎免疫可显著阻止红细胞免疫功能的下降。此外,胚免组鸡血清中红细胞免疫粘附抑制因子活性下降（IC形成减少）,使得红细胞免疫功能得以更好的发挥。     

四川省毒害植物——紫茎泽兰调查报告

紫茎泽兰是恶性毒害植物,已传入攀枝花市和凉山州两地１４县３区１市,分布面积３７５１６０ｈｍ２,每年以２０～３０ｋｍ的速度向北向东传播。其繁殖能力和侵占性极强,是农、林、牧业生产的大敌,应采取切实可行的方法,发动群众,人人动手,把防除与开发利用结合起来,开展综合防治。     

鸭疫里氏杆菌免疫原性研究Ⅰ.荚膜的形态学观察及荚膜物质的提取

本研究应用电镜对Ⅰ型鸭疫里氏杆菌的荚膜进行了初步观察,在电镜下,负染的菌体周围呈一电子透明层;未经抗体处理的细菌超薄切片,荚膜在菌体周围呈松散的絮状结构,而应用同源抗体处理后,荚膜则呈一均匀层。同时在超薄切片中还看到细菌的空泡和外膜结构。采用十六烷基三甲基溴化铵（ＣＴＡＢ）成功地提取了培养物上清液中的荚膜成分,经过测定比较,加入５ｍＭＣＴＡＢ时效果最佳。     

中华蜜蜂保种(资源)分子生物学评价方法

中华蜜蜂是我国独特的东方蜜蜂遗传资源,具有非常重要的利用与保护价值。随着技术的发展,基于分子生物学的保种手段也逐渐展开。本文详细阐述了蜜蜂保种的重要性,介绍了适用于蜜蜂资源评价的常用遗传标记以及适用于遗传资源保护的评价指标,为中蜂资源保护与利用工作提供理论与实践指导。     

热应激对动物体内蛋白质周转代谢的内分泌途径的影响

本文讨论了高温条件下机体内分泌激素对蛋白质合成、分解的影响,试图从内分泌角度解释高温对动物体蛋白质沉积的影响机理.     

A miniature lighted stylet for fast oral endotracheal intubation in rabbits

Efficient oral endotracheal intubation of laboratory animals is a challenging technique in veterinary research. This study introduces a miniaturized lighted stylet for rabbit intubation. An experiment with repeated measures on two factors was used to assess the feasibility and efficacy of this method. The first factor compared stylet intubation vs. laryngoscopic intubation. The second compared three practitioners, one with prior experience and two without. Success rates on the initial attempt were not statistically different (χ² = 2.46, P = 0.12). The time difference between methods was significant (F = 41.007, P < 0.001), although the effect of practitioners was not (F = 1.038, P = 0.365). The mean ± SD of the intubation time, combining results from the three practitioners, was 20.34 ± 17.15 s for the stylet method and 57.58 ± 64.21 s for the laryngoscopic method. The results of this study demonstrate that lighted stylet intubation is efficient, robust, and independent of practitioner experience.