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81.
82.
J Mendels A Frazer R G Fitzgerald T A Ramsey J W Stokes 《Science (New York, N.Y.)》1972,175(28):1380-1382
A reduction in 5-hydroxyindoleacetic acid in cerebrospinal fluid was found in depressed and manic patients both while they were symptomatic and also after treatment. The concentration of homovanillic acid was initially reduced and then tended to increase after treatment. 相似文献
83.
Historical evidence of acid deposition and its effects which was presented at Muskoka, Ontario in September, 1985 is summarized. This evidence consists of written records of the past chemistry and biology of atmospheric, terrestrial, and aquatic systems; it also includes evidence from archived collections which were “revisited”, from tree rings, and from the chemical and biological “records” in lake sediment and peat from which histories of airborne contaminants and ecosystem responses to these contaminants were inferred. 相似文献
84.
S Saraswat SD Kharche PK Rout R Pawaiya C Gangwar DK Swain R Kaushik 《Reproduction in domestic animals》2020,55(9):1080-1092
Estrogen and its receptors are essential for sexual development and reproduction. Oestrogen receptor alpha (ERα) is a nuclear receptor activated by the hormone oestrogen. In male, ERα is encoded by the gene ESR1 (oestrogen receptor1) responsible for better fertility. ESR1 is involved in the reabsorption of luminal fluid during the transit of spermatozoa from the testis to the head of the epididymis which is important for their survival and maturation during epididymal storage. The absence of ESR1 leads to reduced epididymal sperm content, reduced sperm motility and fertilizing ability. The present study was undertaken to investigate the expression and presence of ESR1 gene in fertile and low-fertile male goat breeds. We identified ESR1 gene through various molecular tools. Genotyping was carried out by high resonance melting analysis using Roche Light Cycler 480(LC-480) system and found three different genotypes. Genotypic frequency-AA (blue-0.67), BB(Red-0.2), AB(Green-0.08) with allele frequency A(0.71 and B (0.29). The predominance of this gene in head of epididymis in fertile bucks was confirmed by SDS-PAGE, Western blotting and immunohistochemistry. From the results, we corroborated that the present study provides a useful and effective way to predict male fertility in goat breeds, which in turn increases the percentage of fertility in flock leading to more number of offspring in a kidding season. 相似文献
85.
Attempts have been made to correlate the chemical structure of 38 pyridine derivatives, including two herbicides, picloram (Tordon) and Daxtron and a nitrification inhibitor (N-Serve), with their resistance to attack by soil microorganisms under aerobic and anaerobic conditions and with their herbicidal effect upon lucerne (Medicago sativa L., Hunter River). 相似文献
86.
Fellman CL Stokes JV Archer TM Pinchuk LM Lunsford KV Mackin AJ 《Veterinary immunology and immunopathology》2011,140(3-4):175-180
Cyclosporine is a powerful immunosuppressive drug that is being used with increasing frequency to treat a wide range of immune-mediated diseases in the dog. To date, ideal dosing protocols that will achieve immunosuppression with cyclosporine in dogs remain unclear, and standard methods that can measure effectiveness of immunosuppression have not been established. The aim of our study was to evaluate the effects of in vitro cyclosporine exposure on a panel of molecules expressed by activated T cells to ascertain their potential as biomarkers of immunosuppression in dogs. Blood was drawn from six healthy dogs, and peripheral blood mononuclear cells (PBMC) were isolated and activated. Half of the cells were incubated with 200 ng/mL cyclosporine prior to activation, and the other half were not exposed to cyclosporine. Samples were analyzed using flow cytometry, and the expression of intracellular cytokines IL-2, IL-4, and IFN-γ was evaluated after 6, 12, and 24h of drug exposure. Each cytokine exhibited a time-dependent suppression profile, and all but two samples activated in the presence of cyclosporine showed lower cytokine expression than untreated controls. We also evaluated the expression of the surface T cell activation molecules CD25 and CD95 by flow cytometry after 36 h of drug exposure. Expression of these surface molecules decreased significantly when activated in the presence of cyclosporine. Our results suggest that suppressed expression of the markers related to T cell activation could potentially be utilized as an indicator of the efficacy of cyclosporine therapy in dogs. 相似文献
87.
Møller SM Whittaker AK Stokes JR Gidley MJ Andersen U Bertram HC 《Journal of agricultural and food chemistry》2011,59(18):10097-10103
The molecular motion of water was studied in glucono-δ-lactone-acidified skim milk powder (SMP) solutions with various pH values and dry matter contents. NMR relaxometry measurements revealed that lowering the pH in SMP solutions affected 17O and 1H T2 relaxation rates almost identically. Consequently, the present study indicates that the proteins present in the samples do not affect the 1H relaxation behavior markedly, even at relatively high SMP concentrations (15-25%). Comparison of rheological measurements and NMR measurements suggested that the collapse of κ-casein during acidification could contribute to the initial decrease in 17O and 1H relaxation rate in the pH range between 6.6 and 5.5 for 15% SMP and in the pH range between 6.6 and 5.9 for 25% SMP. However, below pH 5.5 the viscosity and 17O and 1H NMR relaxation rates did not correlate, revealing that the aggregation of casein micelles, which increases viscosity below pH 5.5, does not involve major repartitioning of water. 相似文献
88.
Intake and digestion in cattle fed warm- or cool-season grass hay with or without supplemental grain
Intake and digestion in cattle fed warm- or cool-season grass hay with or without low-level grain supplementation were studied with a 2 x 2 factorial arrangement of treatments in two 4 x 4 Latin square experiments. In Exp. 1, four cannulated beef cows (396 kg) were given Bermuda grass (B) or orchard grass (OG) hay at 1.5% body weight (BW) with 0 or .3% BW of ground corn (C; dry matter). Bermuda grass contained 12.1% crude protein, 79.3% neutral detergent fiber (NDF) and 5.5% acid detergent lignin (ADL); OG contained 10.6% crude protein, 82.4% NDF and 8.1% ADL. An interaction (P less than .07) between forage type and C supplementation was noted for microbial N entering the duodenum; C supplementation had a positive effect with B (30% increase) and little effect with OG. Corn supplementation did not affect ruminal NDF digestion with B, but it elicited an increase with OG (interaction, P less than .05; means were 60.7, 60.1, 61.5 and 66.3%). In the second experiment, growing dairy steers (196 kg) were given ad libitum access to similar B or OG hays and were fed 0 or .5% BW of C. Dry matter (DM) intake was lower for OG than for B (P less than .05) and was lower with than without C (P less than .06; means were 2.76, 2.56, 2.53 and 2.30% BW for B, BC, OG and OGC, respectively). Total tract organic matter digestion (%) was higher for OG than for B (P less than .10) and was higher with than without C (means were 54.7, 61.5, 60.4 and 65.3%). In conclusion, chemical constituents such as NDF may govern differences in intake between warm- and cool-season grasses, but physical attributes of the forages appear more important to digestion. 相似文献
89.
R N Barker T J Gruffydd-Jones C R Stokes C J Elson 《Veterinary immunology and immunopathology》1992,34(1-2):1-20
A direct enzyme-linked antiglobulin test (DELAT) was used to measure the levels of red blood cell (RBC) bound IgG, IgM, IgA and C3 in dogs with autoimmune haemolytic anaemia (AIHA). At presentation, one or more DELAT parameters was raised in each AIHA case, and the RBC were typically coated with immunoglobulin of more than one class, together with C3. There was no relationship between the levels of RBC-bound IgG, IgM or IgA and the severity of the anaemia, although a significant negative correlation (rs = -0.66, P < 0.02) was found between bound C3 and blood haemoglobin concentration. These results indicate that the level of sensitisation of erythrocytes with IgG alone is not a reliable predictor of the severity of haemolysis in different cases, and that the pathogenesis of AIHA can be complex, involving multiple immunoglobulin classes and complement in the destruction of RBC. A significant relationship (rs = 0.63, P < 0.02) was found between serum IgG concentration and haemoglobin levels, and it is suggested that this may be due to free IgG inhibiting the interaction of IgG-sensitised RBC with macrophages. Serial measurements from individual AIHA cases during treatment revealed that the levels of RBC-bound immunoglobulins fell simultaneously with improvements in anaemia. In one dog, a relapse was associated with increases in bound IgG and IgM. Transient relative reticulocytopenia at presentation was common, but was not related to the severity of the anaemia. However, in other cases there was a persistent failure to increase RBC production, which was associated with slower recovery. 相似文献
90.
L H Makala T Kamada H Nagasawa I Igarashi K Fujisaki N Suzuki T Mikami K Haverson M Bailey C R Stokes P W Bland 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(6):625-636
Leukocyte populations present in the discrete Peyer's patches (PP) of the pig were characterized from birth (Day 0) to day 35 after birth by immunohistochemistry and image analysis. Immediately after birth, cell membrane expression of CD2 and CD3, major histocompatibilty complex (MHC) class 11 (both SLA (swine leukocyte antigen) -DQ+ and SLA-DR+), CD21, 74-22-15 and surface immunoglobulin (sIg) were all demonstrable. Computer assisted morphometric techniques were used to confirm the significant expansion of these cell populations from birth onwards. The distribution of the cell types was not random but suggested a preferential retention of cells at specific sites. This implies a degree of organization of immunological cells within the discrete PP, enhancing the potential to mount immune responses in the most efficient manner. 相似文献