Evaluation of various compounds to inhibit activity of matrix metalloproteinases in the tear film of horses with ulcerative keratitis

OBJECTIVE: To examine in vitro effects of various antiproteolytic compounds on activity of matrix metalloproteinase (MMP)-2 and -9 in the tear film of horses with active corneal ulcers. SAMPLE POPULATION: Samples of tear film obtained from the eyes of 34 horses with active ulcerative keratitis. PROCEDURE: Horses were sedated, and tear samples were collected from the lower fornix of 34 ulcerated eyes by use of capillary tubes. The protease inhibitors 0.2% EDTA, 0.1% doxycycline, 10% N-acetylcysteine (NAC), 0.1% solution of a modified dipeptide that contains hydroxamic acid (ie, ilomostat), 0.1% alpha1-proteinase inhibitor (PI), 0.5% alpha1-PI, and 100% fresh equine serum (ES) were used to treat pooled samples. Amount of latent and active MMP-2 and -9 was measured by optical density scanning of gelatin zymograms of treated and untreated tear samples. RESULTS: Pooled tear samples obtained from ulcerated eyes contained the latent and active forms of MMP-2 and -9. Compared with MMP activity in untreated samples, total MMP activity (sum of all bands detected) observed on the gelatin zymogram gels was reduced by 99.4% by EDTA, 96.3% by doxycycline, 98.8% by NAC, 98.9% by ilomostat, 52.4% by 0.1% alpha1-PI, 93.6% by 0.5% alpha1-PI, and 90.0% by ES. CONCLUSIONS AND CLINICAL RELEVANCE: We documented that EDTA, doxycycline, NAC, ilomostat, alpha1PI, and ES inhibited MMP activity in vitro. Because these compounds use different mechanisms to inhibit various families of proteases in the tear film of horses, a combination of these protease inhibitors may be beneficial for treatment of corneal ulcers in horses.     

Chemical and organoleptic changes in poultry meat resulting from the growth of psychrophylic spoilage bacteria at 1 degree C. I. Introduction and changes in free amino acids

Changes in pH and in the free amino acid content of chicken breast and leg muscle inoculated with micro‐organisms in pure or mixed culture and stored at 1° C have been measured. Control experiments with aseptically prepared antibiotic‐treated samples separated the effects of autolysis from those attributable to the bacteria. A pigmented strain of Pseudomonas and Pseudomonas putrefaciens at counts up to 10⁸ organisms/g produced smaller effects than autolysis, but somewhat larger changes resulted from the growth to 10⁹/g of the pigmented Pseudomonas and, particularly, of a mixed culture of the two bacteria with three others normally present on chill‐stored chicken. The possible effects of these changes on flavour are discussed.     

Management strategy influences landscape patterns of high-severity burn patches in the southwestern United States

Landscape Ecology - Spatial patterns of high-severity wildfire in forests affect vegetation recovery pathways, watershed dynamics, and wildlife habitat across landscapes. Yet, less is known about...     

Measuring Soil and Tissue Potassium with a Portable Ion-Specific Electrode in Cotton

Most laboratories make potassium (K) fertilizer recommendations based on field research calibrated with soil K from atomic absorption spectroscopy, flame emission spectroscopy, or inductively coupled plasma mass spectrometry. Information is needed to interpret readings from K⁺ meters on cotton. The objective was to compare soil and cotton plant sap potassium content from a Laqua Twin? K⁺ meter to results from standard tests. Aluminum sulfate solution was used with the meter to extract K from 10 soil samples from the North America Proficiency Testing program. A linear relationship (R² = 0.86) was found between K measured with a K⁺ meter and K measured with 138 labs using ammonium acetate or Mehlich-3. Using a factor of 1.4754 to adjust readings, fertilizer recommendations with the K⁺ meter were similar to results from atomic absorption spectroscopy. A linear relationship was found between petiole K measured in the lab and petiole sap measured with the K⁺ meter.     

Farm-level factors associated with above-average production on pig farms in Evia, Greece

The aim of this study was to identify farm-level factors associated with above-average production of pig farms in Evia, Greece. Cross-sectional data on pig farm characteristics and management, farmer characteristics, worming practices, anthelmintics used, and the parasite loads of 10 randomly selected boars, sows, weaners, and fatteners were collected from 28 pig enterprises in the Evian region of Greece (74% of the total number of pig enterprises in this region). Above-average productivity was defined as weaning 18 or more piglets per sow per year. A multivariate logistic regression model found that employing immigrant workers reduced the odds of a farm being an above-average producer (OR 0.016, 95% CI 0.001–0.27), while having a veterinarian select the anthelmintic strategy significantly increased the odds of a farm being an above-average producer (OR 10.24, 95% CI 0.78–135.13). None of the gastrointestinal parasites under investigation were significantly associated with above-average productivity. It is concluded that despite the fact that endoparasite load was not significantly associated with above-average production, a quantification of the impact of intestinal worms on pig productivity is needed to know how much control can be envisaged for gastrointestinal worms before reaching the threshold at which it is no longer physically or financially reasonable to continue pursuing control.     

Cytochemical reactions in cells from leukemic dogs

Leukemic cells from 17 dogs with spontaneous leukemia were stained with leukocyte alkaline phosphatase, alpha naphthyl acetate esterase with and without fluoride, peroxidase, and periodic acid-Schiff. Cytochemistry was necessary for identification or confirmation of leukemic cell type in most dogs and resulted in changing the light microscopic morphologic diagnosis in eight of 17 dogs. Leukemic cell types diagnosed were myelomonocytic leukemia in seven dogs, monocytic leukemia in five dogs, lymphocytic leukemia in four dogs, and myelocytic leukemia in one dog.