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71.
Yuan Meng-yi Chang Di Xie Tong-tong Sheng Xi-hui Feng Zi-yan Fu Bo-fan Li Qian-ru Liu Fang Guo Yong Ding Jin Wang Xiang-guo Ni He-min 《东北农业大学学报(英文版)》2021,28(1):61-74
WNT7A and β-catenin localisations and roles in regulating periimplantation ovine conceptus development under natural estrous conditions have been elaborated.However,their locations and expression patterns have not been reported under induction of oestrus.The localisation,expression and function of WNT7A and β-catenin in the uterine tissues of the early pregnant and non-pregnant sheep on days 10,12,14,16 and 18 following artificial induction of oestrus were investigated by means of in situ hybridisation,real-time RT-PCR,immuno-histochemistry and western blotting methods.WNT7A and β-catenin mRNA and protein were both restricted to the apical surfaces of the uterine luminal epithelium (LE) and glandular epithelium (GE).In pregnant sheep,protein localisation of WNT7A and β-catenin was observed both in the endometrial LE and GE.Their staining presented on day 10,increased between day 12 and day 16,and decreased on day 18.WNT7A and β-catenin mRNA and protein expression increased initially and then decreased from day 10 to day 18,peaking on day 16,and β-catenin reaching a peak on day 18 in the uterine tissues of pregnant sheep (p0.05).By contrast,no significant changes in WNT7A and β-catenin mRNA and protein expression levels were observed from day 10 to day 18 of the oestrus cycle in the uterine tissues of non-pregnant sheep (p0.05).Additionally,WNT7A and β-catenin mRNA and protein expression levels in the uterine tissues of the early pregnant sheep were significantly higher than those of non-pregnant sheep (p0.05).Treatment of endometrial epithelial cells with WNT7A increased the mRNA expressions of β-catenin,c-myc and Cyclin D1.These results provided an underlying mechanism of periimplantation ovine conceptus development under induction of oestrus. 相似文献
72.
针对目前池塘养殖中投饵机大部分加料都是依靠人力完成,劳动强度大,在陡峭斜岸上对投饵船进行加料困难的现状,设计一种无动力出料的节能定量加料车。加料车使用旋转下料阀进行定量下料,使用剪叉式升降平台调整加料高度。通过设计位于下料阀出料口的球形限位旋转连接件调整加料管的倾角实现无动力出料。基于圆柱凸轮设计新型伸缩加料管。加料车通过车体的移动、剪叉式升降平台的升降、加料管倾角和长度的调整实现定向、定距、定点的饲料添加。制作加料车样机,以投饵船和固定式投饵机为加料目标进行加料试验,试验结果表明加料车对不同规格饲料具有通用性,1.2 m的伸缩长度和1.5 m的升降高度能满足投饵船和固定式投饵机的加料需求,调整灵活,加料速度为6.2 kg/min。设计的加料车使用灵活,能代替人工给投饵船和固定式投饵机加料,减轻人工劳动强度,提高养殖过程的机械化水平,为进一步实现自动化和智能化打下基础。 相似文献
73.
A. Di Francesco I. Cameldi F. Neri L. Barbanti A. Folchi A. Spadoni E. Baraldi 《Plant pathology》2019,68(8):1525-1532
Bull’s eye rot is a typical quiescent postharvest apple disease in major fruit-growing areas. The susceptibility of different apple cultivars to Neofabraea spp. (N. vagabunda and N. malicorticis) was assessed, with Granny Smith showing the most resistance and Cripps Pink the most susceptibility. To assess the factors involved in conidial germination, Neofabraea spp. were grown on crude protein extracts (CPEs) collected from apple fruits at different storage periods. Fungal germ tube growth rate and pathogenic enzyme (cellulase and xylanase) activity were assessed. Results showed that CPEs collected after 2 and 4 months of storage progressively stimulated conidial germination and germ tube elongation, while a lesser effect was observed from CPEs after 1 month of storage. Xylanase proved to be the main degrading enzyme secreted by all the isolates, while cellulase was produced only by N. vagabunda isolates. Overall, the isolate ID02 was the most virulent, based on more rapid germ tube elongation and greater activity of the lytic enzymes. 相似文献
74.
75.
为了探究低温等离子体(Non-Thermal Plasma,NTP)耦合催化剂对粮仓中磷化氢(PH3)的去除效果,该文采用介质阻挡放电低温等离子体与催化剂耦合的反应装置,研究了NTP与Fe2O3催化剂耦合对PH3去除率的影响,并对耦合反应后的催化剂进行表征,分析产物及反应机理。结果表明,载体和催化剂与NTP耦合改变了NTP的放电状态,提高了PH3去除率;不同载体负载Fe2O3后,在输入功率超过53 W时,NTP与Fe2O3催化剂耦合对PH3去除率均高于无填充,Fe2O3/Al2O3与NTP耦合效果最好,去除效率达到了100%。耦合前后催化剂的表征结果表明,耦合反应之后,Fe2O3/Al2O3催化剂的比表面积降低了11.23%,Fe3+/(Fe2++Fe3+)含量提高了21.06%,Osur/(Olatt+Osur+Oads)含量减少了2.24%,Fe含量降低了4.69%,P含量增加了4.34倍。产物分析表明耦合后生成的主要产物为磷酸。研究结果表明NTP耦合Fe2O3/Al2O3催化剂具有很好的PH3去除效果,为粮食仓储行业去除磷化氢提供理论依据。 相似文献
76.
为探究云南普洱地区有机茶身份辨识特征,完善高原有机茶身份辨识技术,选取云南省普洱市的5个代表性有机种植认证茶园(ZX、LS、CYT、YS、HL)和相邻的5个常规茶园(C-1、C-2、C-3、C-4、C-5),采集300余份茶叶和土壤样本,结合稳定同位素比例质谱仪、元素分析仪等测定茶叶和土壤的δ15N丰度、δ13C丰度、全氮、全碳、有机碳、碱解氮等指标,通过因子分析和判别分析的方法构建了有机茶叶同位素-土壤性质判别模型,并使用Holdout法进行验证。结果表明:不同茶园有机茶叶与相邻常规茶叶相比未表现出一致的δ15N,δ13C值差异规律,因此,使用单一稳定同位素因子难以鉴别有机茶。通过进一步对茶叶和土壤性质进行因子分析,筛选出6个适合判别的特征向量,构建了基于同位素特征和土壤性质的多元有机判别模型,其判别函数方程为:y=-0.081(茶叶δ15N)+0.819(茶叶δ13C)+0.093(土壤全氮)+2.117(土壤全碳)-0.155(土壤有机碳)+2.870(土壤pH)+4.232,y< ![]()
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0.5为常规茶,判别准确度为92.3%。综上,基于同位素特征值和土壤性质参数指标构建的多元有机判别模型可实现云南省普洱市高原有机茶身份的准确判别。 相似文献
77.
78.
Rodkhum C Hirono I Stork M Di Lorenzo M Crosa JH Aoki T 《Journal of fish diseases》2006,29(3):157-166
The genome of Vibrio anguillarum strain H775-3 was partially determined by a random sequencing procedure. A total of 2,300 clones, 2,100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence-related genes were identified, 36 of which are novel in V. anguillarum, including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes. 相似文献
79.
Domitilla Pulcini Tommaso Russo Paola Reale Alexia Massa‐Gallucci Grace Brennan Stefano Cataudella 《Aquaculture Research》2014,45(3):397-409
Prolonged exposure to captive conditions has led to the development of a rainbow trout ‘farmed’ phenotype, which is different from that of wild trout. Selection for desirable productive traits in hatcheries has resulted in the development of some morphological traits that are maladaptive in nature. The recent development of organic aquaculture, guided by the well‐being of the fish, could potentially produce a new farmed phenotype that would be more adaptive in nature. In this study, rainbow trout reared in intensive and organic farms were compared by means of shape analysis, to detect patterns of shape variation associated with rearing environment. The results of this study highlight a significant effect of the rearing method on rainbow trout shape: organically reared trout showed a higher body profile, in particular in the head and trunk regions, shorter median fins and a deeper caudal peduncle. A combined effect of density and habitat complexity could have contributed to the observed shape differences: in organic rearing systems, lower densities and steady water could increase territoriality and aggressive interactions, promoting body designs more functional in rapid attacks and escapes. 相似文献
80.