全文获取类型
收费全文 | 212篇 |
免费 | 12篇 |
专业分类
林业 | 7篇 |
农学 | 16篇 |
基础科学 | 1篇 |
72篇 | |
综合类 | 6篇 |
农作物 | 27篇 |
水产渔业 | 14篇 |
畜牧兽医 | 54篇 |
园艺 | 2篇 |
植物保护 | 25篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 8篇 |
2020年 | 13篇 |
2019年 | 5篇 |
2018年 | 7篇 |
2017年 | 9篇 |
2016年 | 7篇 |
2015年 | 14篇 |
2014年 | 8篇 |
2013年 | 11篇 |
2012年 | 13篇 |
2011年 | 20篇 |
2010年 | 12篇 |
2008年 | 18篇 |
2007年 | 15篇 |
2006年 | 10篇 |
2005年 | 8篇 |
2004年 | 9篇 |
2003年 | 5篇 |
2002年 | 6篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1989年 | 1篇 |
1973年 | 1篇 |
1971年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有224条查询结果,搜索用时 0 毫秒
21.
22.
Rita M. De Miccolis Angelini Wassim Habib Caterina Rotolo Stefania Pollastro Francesco Faretra 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(2):185-199
Resistance to the fungicide boscalid in laboratory mutants of Botryotinia fuckeliana (Botrytis cinerea) was investigated. The baseline sensitivity to boscalid was evaluated in terms of colony growth (EC50 = 0.3–3 μg ml−1; MIC = 10–30 μg ml−1) and conidial germination (EC50 = 0.03–0.1 μg ml−1; MIC = 1–3 μg ml−1) tests. Mutants were selected in vitro from wild-type strains of the fungus on a fungicide-amended medium containing acetate as a carbon source. Mutants showed
two different levels of resistance to boscalid, distinguishable through the conidial germination tests: low (EC50 ∼ 0.3 μg ml−1, ranging from 0.03 to 1 μg ml−1; MIC > 100 μg ml−1) and high (EC50 > 100 μg ml−1) resistance. Analysis of meiotic progeny from crosses between resistant mutants and sensitive reference strains showed that
resistant phenotypes were due to mutations in single major gene(s) inherited in a Mendelian fashion, and linked with both
the Daf1 and Mbc1 genes, responsible for resistance to dicarboximide and benzimidazole fungicides, respectively. Gene sequence analysis of
the four sub-units of the boscalid-target protein, the succinate dehydrogenase enzyme, revealed that single or double point
mutations in the highly conserved regions of the iron-sulphur protein (Ip) gene were associated with resistance. Mutations
resulted in proline to leucine or phenylalanine replacements at position 225 (P225L or P225F) in high resistant mutants, and
in a histidine to tyrosine replacement at position 272 (H272Y) in low resistant mutants. Sequences of the flavoprotein and
the two transmembrane sub-units of succinate dehydrogenase were never affected. 相似文献
23.
We report three cases of adult cats showing a prolapse of the gland of the third eyelid. Three different breeds were affected: Burmese, Persian and Domestic Short-haired. In all cases, the disorder occurred spontaneously, without any other ocular sign. Surgical replacement of the gland was performed using the Morgan pocket technique. Good esthetic results were obtained, and no recurrence occurred. 相似文献
24.
Napolitano A Lanzuise S Ruocco M Arlotti G Ranieri R Knutsen SH Lorito M Fogliano V 《Journal of agricultural and food chemistry》2006,54(20):7863-7869
Nutritionists recommend increasing the intake of soluble dietary fiber (SDF), which is very low in most cereal-based products. Conversion of insoluble DF (IDF) into SDF can be achieved by chemical treatments, but this affects the sensorial properties of the products. In this study, the possibility of getting a substantial increase of SDF from cereal products using a tailored preparation of Trichoderma enzymes is reported. Enzymes were produced cultivating Trichoderma using durum wheat fiber (DWF) and barley spent grain (BSG) as unique carbon sources. Many Trichoderma strains were screened, and the hydrolysis conditions able to increase by enzymatic treatment the amount of SDF in DWF and BSG were determined. Results demonstrate in both products that it is possible to triple the amount of SDF without a marked decrease of total DF. The enzymatic treatment also causes the release of hydroxycinnamic acids, mainly ferulic acid, that are linked to the polysaccharides chains. This increases the free phenolic concentration, the water-soluble antioxidant activity, and, in turn, the phenol compounds bioavailability. 相似文献
25.
De Miccolis Angelini RM Rotolo C Masiello M Pollastro S Ishii H Faretra F 《Pest management science》2012,68(9):1231-1240
BACKGROUND: QoI fungicides, inhibitors of mitochondrial respiration, are considered to be at high risk of resistance development. In several phytopathogenic fungi, resistance is caused by mutations (most frequently G143A) in the mitochondrial cytochrome b (cytb) gene. The genetic and molecular basis of QoI resistance were investigated in laboratory and field mutants of Botryotinia fuckeliana (de Bary) Whetz. exhibiting in vitro reduced sensitivity to trifloxystrobin. RESULTS: B. fuckeliana mutants highly resistant to trifloxystrobin were obtained in the laboratory by spontaneous mutations in wild‐type strains, or from naturally infected plants on a medium amended with 1–3 mg L?1 trifloxystrobin and 2 mM salicylhydroxamic acid, an inhibitor of alternative oxidase. No point mutations were detected, either in the complete nucleotide sequences of the cytb gene or in those of the aox and Rieske protein genes of laboratory mutants, whereas all field mutants carried the G143A mutation in the mitochondrial cytb gene. QoI resistance was always maternally inherited in ascospore progeny of sexual crosses of field mutants with sensitive reference strains. CONCLUSIONS: The G143A mutation in cytb gene is confirmed to be responsible for field resistance to QoIs in B. fuckeliana. Maternal inheritance of resistance to QoIs in progeny of sexual crosses confirmed that it is caused by extranuclear genetic determinants. In laboratory mutants the heteroplasmic state of mutated mitochondria could likely hamper the G143A detection, otherwise other gene(s) underlying different mechanisms of resistance could be involved. Copyright © 2012 Society of Chemical Industry 相似文献
26.
Latrofa MS Annoscia G Dantas-Torres F Traversa D Otranto D 《Veterinary parasitology》2012,184(2-4):267-270
The present study reports two simple molecular approaches allowing a rapid identification of the most prevalent species of phlebotomine sand flies in the Mediterranean region. A PCR protocol for the amplification of ITS2 ribosomal region and a PCR-RFLP on a mitochondrial DNA fragment (cytb-nd1) were settled in order to identify and discriminate among Phlebotomus perniciosus, Phlebotomus neglectus, Phlebotomus perfiliewi, Phlebotomus papatasi and Sergentomyia minuta. The ITS2 regions showed a certain degree of interspecific variability, which led to PCR amplicons of different sizes, i.e., 450, 490, 460, 480 and 530 bp for P. perniciosus, P. neglectus, P. perfiliewi, P. papatasi, and S. minuta, respectively. Analogously, the digestion of a mitochondrial DNA amplicon with Ase I enzyme showed five different restriction profiles, which allowed the unequivocal differentiation of the sand fly species examined. These methods might represent useful tools for a molecular large scale screening of phlebotomine sand fly species caught in areas where leishmaniasis is endemic, in order to plan appropriate epidemiological surveillance programs for both Leishmania spp. and their vectors. 相似文献
27.
Latrofa MS Dantas-Torres F Annoscia G Genchi M Traversa D Otranto D 《Veterinary parasitology》2012,185(2-4):181-185
This study describes a duplex real-time polymerase chain reaction (PCR) assay for the detection and differentiation between Dirofilaria immitis and Dirofilaria repens in dog blood and mosquitoes. Regions of a cytochrome oxidase 1 (cox1) mitochondrial DNA fragment and the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA were amplified from microfilariae and adult worm samples, using a sensitive SsoFast? EvaGreen(?) based real-time PCR method coupled with melting-curve analysis. The limit of the real-time PCR in detecting microfilaria and adult worm DNA was also tested both in dog blood and in artificially infected microfilarial. Two peaks at different melting temperatures (T(m)) for D. immitis (mean ± SD=75.7 ± 0.3°C) and D. repens (mean ± SD=70 ± 0.7°C), respectively, were obtained for microfilarial and adult positive controls of both species when examined separately and together. The real-time PCR protocol was also efficient in detecting microfilarial and adult DNA of both species when tested in samples spiked with DNA from Aedes albopictus, in Aedes aegypti experimentally infected by D. repens and in Culex pipiens naturally infected by D. repens and D. immitis. The high sensitivity of real-time PCR confirmed its reliability in detecting small amounts of genomic DNA either in dog blood or mosquitoes (2.5 pg/μl and 3 × 10(-1)pg/μl for D. immitis and D. repens, respectively). This assay is proposed as a tool for the epidemiological surveillance of the two most important Dirofilaria species in areas where they are endemic and sympatric. 相似文献
28.
Staffan Berg Janine Fischbach Franka Brüchert Mikael Poissonnet Stefania Pizzirani Anne Varet Udo H. Sauter 《European Journal of Forest Research》2012,131(1):81-94
The forest-based sector has been at the forefront in operationally implementing the sustainability concept, its associated
principles and indicators for sustainable forest management. Several methods have been developed to study environmental impacts
of forestry activities, but none of the existing tools address all the dimensions of sustainability along the whole forest
wood chain (FWC) in a balanced way. Consequently, the decision was made to develop a tool for sustainability impact assessment
(ToSIA), the modelling framework for sustainability impact assessment of FWCs. The objective of the EU Project Eforwood was
to develop ToSIA, a decision support tool. Within ToSIA, a FWC is modelled as a number of interconnected processes. For each
process, a range of economic, environmental and social indicators and their respective values are calculated, thus representing
the three pillars of sustainability. By this method, the multifunctionality of forests can be assessed and supply chains can
be compared with respect to sustainability. Sensitivity analysis and scenario techniques can be applied to learn about the
effect of expected changes to the structure of the chain, the material flows and the indicator values. In order to provide
the tool with information about forest and logging operations, data were collected at two fundamental levels: (1) a regional
level with case studies in Scandinavia, Iberia and Baden-Württemberg and (2) a European level with a case study that reflects
conditions in the 27 countries of the European Union. This paper describes and details the harvesting and logging processes
for the European countries. The results are displayed for each of the three regional case studies as well as aggregated to
five principal areas in Europe: Eastern, Northern, Western, Central and Southwest Europe. 相似文献
29.
Detection of Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) by polymerase chain reaction (PCR) is often hampered by the lack of efficient methods for sample treatment. We report a protocol for analysis of faecal samples based on buoyant density centrifugation in Percoll and IS900 sequence capture PCR combined with a dot blot assay for detection of low-grade infection of M. paratuberculosis. Serial dilutions of M. paratuberculosis genomic DNA and M. paratuberculosis bacteria were used to assess the sensitivity of the method. The final evaluation was performed with spiked faecal samples, which also were analysed by culture. The presence of PCR inhibitory substances in processed faecal samples was evaluated by including a PCR internal control. By using buoyant density centrifugation, sequence capture PCR, and dot blot hybridisation, we achieved a sensitivity of 10(3)CFU (colony forming units)/g of faeces. The detection limit by culture was assessed to 10(2)CFU/g of faeces. We conclude that the described protocol is a fast and sensitive alternative to bacterial culture of faecal samples. 相似文献
30.
Berhane Lakew Robert J. Henry Salvatore Ceccarelli Stefania Grando Jason Eglinton Michael Baum 《Euphytica》2013,189(1):9-29
Associations between markers and drought related traits were investigated on a set of 57 advanced barley breeding lines, carrying various levels of introgression from Hordeum spontaneum lines 41-1 and 41-5, the best sources of drought tolerance in the ICARDA barley breeding program, using 74 simple sequences repeats (SSR) and 20 single nucleotide polymorphism markers. The 57 lines were evaluated for grain yield and drought related traits for three years (2003/04, 2004/05, 2005/06) in nine Mediterranean low rainfall environments. A high level of polymorphism was found with SSR markers, and the mean polymorphism information content and gene diversity values were 0.67 and 0.71, respectively. The number of alleles per locus varied from 2 to 11, with an average of 5.8 alleles per marker. Considering all the 57 lines, the linkage disequilibrium (LD) analysis was significant at a comparison-wise P?<?0.01 level in nearly 9 % of the SSR marker pairs used and a decay of LD was observed to a value of r 2?<?0.2 at a genetic distance of 40?cM. The association analysis revealed a total of 147 significant marker?Ctrait associations for grain yield and drought related traits. A total of 72 (49 %) marker?Ctrait associations showed favorable effects of the exotic germplasm where the H. spontaneum lines contributed to an improvement of the trait under drought stress conditions. The number of significant marker?Ctrait associations per trait were: 12 for growth habit; 2 for growth vigor; 11 for peduncle extrusion; 5 for number of grains per spike; 20 for peduncle length; 16 for days to heading; 20 for plant height; 8 for spike length; 17 for thousand kernel weight; 30 for grain yield; 4 for harvest index and 2 for biological yield. The phenotypic variation explained by individual marker?Ctrait associations ranged from 7.6 % to 36.2 %. The identification of genomic regions associated with grain yield and drought related traits is useful for the genetic improvement of cultivars better adapted to drought stress environments. Thus, the present study is encouraging in identifying significant marker?Ctrait associations through LD based association mapping analysis, which could complement and augment previous quantitative trait loci information for the potential use of Marker Assisted Selection for drought. 相似文献