Screening of dialkoxybenzenes and disubstituted cyclopentene derivatives against the cabbage looper, Trichoplusia ni, for the discovery of new feeding and oviposition deterrents

The antifeedant, oviposition deterrent, and toxic effects of dialkoxybenzene minilibraries and of disubstituted cyclopentene minilibraries (i.e., consisting of four to five compounds) along with their pure constituent compounds were assessed against third instar larvae and adults of the cabbage looper, Trichoplusia ni, in laboratory bioassays in a search for new insect control agents. These compounds mimic naturally occurring bioactive odorants and tastants and are relatively easily prepared from commodity chemicals. Most of these libraries strongly deterred larval feeding, with some exhibiting strong toxic and oviposition deterrent effects as well. Our results suggest some structure-function relationships within these libraries. Replacement of a methyl group with larger alkyl substituents increased the feeding deterrent effects in most cases. The presence of a free hydroxyl group, irrespective of the carbon framework or alkyl substituent, served to reduce feeding deterrent effects in all series of compounds. Further, exceeding a certain group size also generally had a detrimental effect. This information will be useful in designing new insect control agents for agriculture. Some of these libraries and compounds may have potential for development as commercial insecticides.     

Analysis of potential yields and yield gaps of rainfed soybean in India using CROPGRO-Soybean model

To assess the scope for enhancing productivity of soybean (Glycine max L. Merr.), the CROPGRO-Soybean model was calibrated and validated for the diverse soybean-growing environments of central and peninsular India. The validated model was used to estimate potential yields (water non-limiting and water limiting) and yield gaps of soybean for 21 locations representing major soybean regions of India. The average water non-limiting potential yield of soybean for the locations was 3020 kg ha⁻¹, while the water limiting potential was 2170 kg ha⁻¹ indicating a 28% reduction in yield due to adverse soil moisture conditions. As against this, the actual yields of locations averaged 1000 kg ha⁻¹, which was 2020 and 1170 kg ha⁻¹ less than the water non-limiting potential and water limiting potential yields, respectively. Across locations the water non-limiting potential yields were less variable than water limited potential and actual yields, and strongly correlated with solar radiation during the season (R² = 0.83, p ≤ 0.01). Both simulated water limiting potential yield (R² = 0.59, p ≤ 0.01) and actual yield (R² = 0.33, p ≤ 0.05) had significant but positive and curvilinear relationships with crop season rainfall across locations. The gap between water non-limiting and water limiting potential yields was very large at locations with low crop season rainfall and narrowed down at locations with increasing quantity of crop season rainfall. On the other hand, the gap between water limiting potential yield and actual farmers yield was narrow at locations with low crop season rainfall and increased considerably at locations with increasing amounts of rainfall. This yield gap, which reflects the actual yield gap in rainfed environment, is essentially due to non-adoption of improved crop management practices and could be reduced if proper interventions are made. The simulation study suggested that conservation of rainfall and drought resistant varieties in low rainfall regimes; and alleviation of water-logging and use of water-logging tolerant varieties in high rainfall regimes will be the essential components of improved technologies aimed at reducing the yield gaps of soybean. Harvesting of excess rainfall during the season and its subsequent use as supplemental irrigation would further help in increasing crop yields at most locations.     

Evaluation of somaclonal variation for genetic improvement of patchouli (<Emphasis Type="Italic">Pogostemon patchouli</Emphasis>), an exclusively vegetatively propagated aromatic plant

Patchouli (Pogostemon patchouli) is an important, exclusively vegetatively propagated aromatic plant, whose essential oil is widely used in perfumery and cosmetic products. Forty SC1 generation (first generation following in vitro phase) somaclones selected randomly from about 400 somaclones developed from the variety Johore, were multiplied through stem cuttings and evaluated in SC₂ and SC₃ generations to study the extent of somaclonal variation generated for plant height, herb yield, essential oil content, essential oil yield, and seven constituents of the essential oil. Significant or highly significant somaclonal variation was observed for plant height, herb yield, essential oil content, essential oil yield, and contents of patchouli alcohol, α-guaiene, α,δ-patchoulene, and α-bulnesene in the essential oil. The number of somaclones significantly superior to the parental variety for plant height, herb yield, essential oil content, and patchouli alcohol content in the essential oil ranged from 8–16 and the maximum superiority over the parental variety for these traits ranged from 21–79%. Broad-sense heritability estimates of plant height, herb yield, and essential oil content were 0.60–0.70 while those of essential oil yield and patchouli alcohol content were 0.44 and 0.47, respectively. Heritability estimates of other studied essential oil constituents were generally low (0.12–0.38). A high positive correlation was observed between essential oil yield and herb yield suggesting that selection for herb yield would be effective in improving essential oil yield. Patchouli alcohol content in the essential oil was negatively correlated with all the studied traits. Somaclonal variation, heritabilities of traits, and inter-trait correlations are reported for the first time in patchouli.     

Lipoxygenase Metabolites of α-linolenic Acid in the Development of Resistance in Pigeonpea, Cajanus cajan (L.) Millsp, Seedlings Against Fusarium udum Infection

Lipoxygenase (LOX) activity was measured in germinating pigeonpea Cajanus cajan seedlings, resistant (ICP-8863) and susceptible (ICP-2376) to wilt fungus, before and after infection with Fusarium udum. LOX activity was significantly higher in the resistant than in the susceptible cultivars of pigeonpea and was enhanced further in response to infection with Fusarium udum. This increase in LOX activity in the resistant cultivars of pigeonpea appears to be due to the induction of lipoxygenase isozymes in response to infection. Analysis of the endogenous LOX metabolites in pigeonpea seedlings revealed the predominant formation of 13-hydroperoxyoctadecadienoic acid (13-HPODE) in healthy seedlings and 13-hydroperoxyoctadecatrienoic acid (13-HPOTrE) in infected seedlings. Further studies on the effects of LOX metabolites on the growth and multiplication of Fusarium udum showed that HPOTrEs, LOX metabolites of -linolenic acid, are more anti-fungal compared to HPODEs, LOX metabolites of linoleic acid.     

Portals of Entry for Gram-Negative Bacteria in a Freshwater Prawn Hatchery

We sought the source of gram-negative bacteria associated with giant freshwater prawns in a hatchery that was previously observed with severe mortality of prawn larvae during the sixth to seventh zoea stages. After thorough disinfection, the gram-negative bacterial microflora of broodstock, larvae, larval rearing tank (LRT) water, and other intake systems were examined for bacteria. Enterobacteriaceae and Vibrio were the major bacteria isolated from the larvae. The present study suggests that Enterobacteriaceae may be introduced via the broodstock and Vibrio via the LRT water.     

Alkali insoluble glucan extracted from Acremonium diospyri is a more potent immunostimulant in the Indian White Shrimp, Fenneropenaeus indicus than alkali soluble glucan

Effect of an extraction method on the structure of glucan and its immunostimulatory response in Fenneropenaeus indicus was investigated. Here we extracted alkali insoluble glucan (AIG) and alkali soluble glucan (ASG) from a filamentous fungi Acremonium diospyri following alkali–acid hydrolysis and the sodium hypochlorite oxidation and dimethyl sulphoxide extraction method respectively. Structural analysis showed that 85% of glucan in AIG was a (1→3)-β- d -glucan and it increased the prophenoloxidase and reactive oxygen intermediate activity when administered to F. indicus . On the other hand, ASG, which contained 93% (1→3)-α-glucan, did not induce significant immune response in shrimp. Here we report that the difference in immunostimulatory potential between AIG and ASG is due to the difference in the percentage of (1→3)-β- d -glucans present in each preparation, which varies with the method of extraction employed. Also our observations suggest that glucan can be used as a potential immunostimulant to shrimp, provided it contains (1→3)-β- d -glucan as the major fraction.     

Effect of potential bioinoculants and organic manures on root‐rot and wilt,growth, yield and quality of organically grown Coleus forskohlii in a semiarid tropical region of Bangalore (India)

Based on earlier results obtained in pot experiments, 2‐year field experiments were conducted with five bioinoculants and neem cake under organic field conditions (with vermicompost as a nutritional supplement) to evaluate their potential to control root‐rot and wilt (a complex problem involving Fusarium chlamydosporum and Ralstonia solanacearum) of the medicinal plant Coleus forskohlii. Plants treated with arbuscular mycorrhizal fungus (Glomus fasciculatum), neem cake or Pseudomonas fluorescens showed significantly increased plant height (15–31%), plant spread (25–33%), number of branches (63–67%) and dry root (129–200%) yields, and reduced disease incidence (47–50%) compared to controls. Increases in yields were reflected by increases in N (51–81%), P (17–76%) and K (44–74%) uptake. The forskolin content of the roots was found not to be affected by any of the bioinoculants, but forskolin yield (calculated) was increased significantly by treatment with G. fasciculatum (227%), neem cake (222%) or P. fluorescens (159%).     

On-farm evaluation of two fast growing trees for biomass production for industrial use in Andhra Pradesh,Southern India

On-farm experiments were conducted in Khammam district of Andhra Pradesh from 2001 to 2006 to evaluate the biomass productivity, intercrop yields and profitability of Eucalyptus tereticornis clonal and Leucaena leucocephala variety K-636 based systems. Trees were planted at a spacing of 3 × 2 m and evaluated at three locations. Height growth was significantly higher in leucaena during the 4 year where as difference in diameter growth was not significant. Biomass partitioning to the bole was high in case of leucaena, ranged from 83% in 2.5–5 cm diameter at breast height (DBH) trees to 89% in 12.5–15 cm DBH trees and in eucalyptus clones the corresponding values were 71% in 2.5–5 cm DBH trees and 83% in 12.5–15 cm DBH trees. Marketable biomass productivity was higher with leucaena (95 Mg ha⁻¹) in comparison to eucalyptus (87 Mg ha⁻¹). Competition effects of trees on intercrops were observed from the 2 year (2002 post-rainy season). Intercrop yields were 45% of the sole crop in eucalyptus system and 36% in leucaena system during the 2 year. Sole eucalyptus and leucaena plantations and intercropping systems recorded higher gross and net returns over arable cropping. Therefore, it can be concluded that leucaena variety K636 and eucalyptus clonal based agroforestry systems are profitable alternatives to arable cropping under rainfed conditions.     

Genetic analysis of bacterial wilt resistance in eggplant (Solanum melongena L.)

European Journal of Plant Pathology - Bacterial wilt triggered by Ralstonia solanacearum is one of the major devastating diseases causing significant yield reduction in eggplant. F1 hybrids (18...