Trace chemicals: the essence of sexual communication systems in heliothis species

Analysis of heptane-soluble compounds from ovipositors of Heliothis zea and Heliothis virescens shows that both species produce relatively large amounts of (Z)-11-hexadecenal, with traces of (Z)-9-hexadecenal, (Z)-7-hexadecenal, and hexadecanal. Heliothis virescens females differ from Heliothis zea in that they also produce trace amounts of tetradecanal, (Z)-9-tetradecenal, and (Z)-11-hexadecen-1-ol. In both species, trace compounds are essential to pheromonal activity and specificity of chemical signals.     

Arthroscopic reattachment of osteochondritis dissecans cartilage flaps of the femoropatellar joint: long-term results

Reasons for performing study: Long‐term efficacy of arthroscopic cartilage reattachment for the treatment of osteochondritis dissecans (OCD) lesions in the equine femoropatellar joint is unknown. Objective: To evaluate radiographic outcome and long‐term performance of horses undergoing OCD reattachment. Hypothesis: Separated OCD cartilage flaps may be reincorporated into the joint surface by reattachment rather than flap removal. Methods: Polydioxanone pins were utilised arthroscopically to reattach OCD lesions in 40 of 44 joints from 27 horses. Cartilage was reattached when it had persisting perimeter continuity, the surface was not deeply fissured or irregular, and the cartilage was not protuberant or extensively mineralised. Bone marrow aspirate concentrate was grafted to additional areas denuded of cartilage or alongside reattached cartilage. Results: Breeds included Thoroughbred (n = 18), Quarter Horse (n = 4), Warmblood (n = 3), Standardbred (n = 1) and Arabian (n = 1). Mean age was 9.7 months. Radiographic lesion length was 1.5–6.3 cm. Reattachment alone was used in 32 of 44 affected joints, a combination of debridement and reattachment in 8 joints and debridement alone in 4 joints. One horse was destroyed due to tendon laceration. Of the remaining 26 horses, mean duration of follow‐up was 15.6 months (range 2 months–12 years). Radiographic resolution of OCD lesions treated with reattachment was significantly improved at 6 months. Twenty horses had long‐term performance data, of which 19 were sound and had reached intended athletic potential. One horse remained lame, and an additional 6 were sound but remained unbroken or were convalescing. Thus, an overall success rate based upon continued soundness in performing horses was 95% (19/20). Conclusion: Cartilage flap reattachment can salvage OCD cartilage by integration with the underlying bone. Potential relevance: Extensive OCD cartilage flaps may be salvaged by reattachment which can result in normal radiographic subchondral bone contour and long‐term athletic performance.     

Assessing leg health in chickens using a force plate and gait scoring: how many birds is enough?

Meat chickens from experimental flocks were tested repeatedly from three to six weeks of age using gait score (GS) and force plate (FP) techniques, and the findings were related to postmortem results for leg health. This initial study indicated that five weeks was the optimal age to test birds using the FP to indicate abnormalities and pathologies. Birds (n=492) with a range of walking styles were then selected at five weeks of age from three commercial flocks, gait scored and tested using a FP. A subsample of these birds (n=191) was examined postmortem, and relationships between leg abnormalities and pathologies, GS and FP results were investigated. Models of leg abnormalities and pathologies with GS or FP measurements as covariates left much variation unexplained; hence, the number of birds that would need to be tested using these methods to assess the flock prevalence of leg abnormalities or pathologies is high.     

Physiological responses of laying hens during whole-house killing with carbon dioxide

1. Poultry on farms are sometimes required to be killed in an emergency, such as during a disease epidemic, yet none of the available methods are ideal. Whole-house carbon dioxide (CO₂) administration has practical advantages, but gives rise to welfare concerns.

2. The study measured the body temperature, respiration, cardiac and brain activity (EEG) responses of 10 adult hens placed in tiered cages in a deep pit house while the entire flock (28,000 end-of-lay hens) was killed with CO₂. Video and thermographic images were also recorded. Liquid CO₂ was injected into the building producing a gaseous concentration of 45% within 19?min.

3. Those hens nearest the gas delivery site showed delayed respiratory, cardiac and EEG responses compared with those at more distant locations. Although sub-zero temperatures were recorded in the immediate vicinity of some birds, body temperatures indicated that they did not die of hypothermia.

4. EEG characteristics strongly associated with unconsciousness were used to determine an unequivocal time to loss of consciousness; this ranged from 6·0 to 10·5 (average 7·8) min after onset of gas injection. Distinctive cardiac and respiratory responses were seen following gas exposure; in particular, birds responded to inhalation of CO₂ by deep breathing.

5. The primary welfare concern is the duration of unpleasant respiratory effects, such as deep breathing, while the birds were substantively conscious. However, the concentration of CO₂ to which the birds were exposed while conscious would not have stimulated nasal and oral nociceptors. Time to death varied between 12·0 and 22·1?min after gas delivery.     

Corollary discharge provides accurate eye position information to the oculomotor system

The saccadic system accurately compensates for perturbations of eye position produced by microstimulation of the superior colliculus. This requires that information about the stimulation-induced change in eye position be provided by an extraretinal source--either proprioceptive endings in extraocular muscles or a centrally generated corollary discharge. It is shown that compensation remains intact after elimination of extraocular muscle proprioception, demonstrating that corollary discharge provides accurate eye position information.     

Cristobalite in volcanic ash of the soufriere hills volcano, montserrat, british west indies

Crystalline silica (mostly cristobalite) was produced by vapor-phase crystallization and devitrification in the andesite lava dome of the Soufriere Hills volcano, Montserrat. The sub-10-micrometer fraction of ash generated by pyroclastic flows formed by lava dome collapse contains 10 to 24 weight percent crystalline silica, an enrichment of 2 to 5 relative to the magma caused by selective crushing of the groundmass. The sub-10-micrometer fraction of ash generated by explosive eruptions has much lower contents (3 to 6 percent) of crystalline silica. High levels of cristobalite in respirable ash raise concerns about adverse health effects of long-term human exposure to ash from lava dome eruptions.     

Pink mould of pecan kernels in the southeastern USA is associated with scab,above-normal temperatures,and humidity during nut maturation

Summary

Pink mould is a disease caused by the common saprophytic fungus Trichothecium roseum (Pers.:Fr.). Pink mould rarely infects kernels of pecan [Carya illinoinensis (Wangeh.) C. Koch] in the southeastern USA, but the disease was a major problem in 2002. T. roseum enters the pecan fruit via scab lesions on the shuck caused by the fungus Fusicladoporium effusum, (G. Winters) Partridge & Morgan-Jones. This study examined the association between pink mould and weather during nut maturation. Average daily temperatures and two or more consecutive days of rainfall were identified as critical factors in 2002, and were compared with weather data between 1963 – 2001 when there was no recorded evidence of pink mould. Both the average daily temperatures and the number of consecutive rainy days during nut maturation were above the 40-year average in 2002. Consecutive rainy days during the nut drying period in 2002 were more than double the 40-year mean, implicating humidity as a critical factor in mould development at this time. Normal variations in temperature were minor factors in disease development following the initial infection. Thus, the onset of mould was associated with the development of scab lesions in late July, a combination of warm temperatures in September and October, and above-average humidity in September and during nut drying from mid-October until mid-November. This combination of factors occurred in only one year out of the past 41 years, explaining the rarity of the disease. The incidence of mould was sigmoidal, with the log-phase overlapping the period of rapid sugar accumulation in the kernel, and potential substrates for the mould to grow.