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61.
Hyun-Joo Kim Mi Jung Kim Eun-Yeong Sim Choon Ki Lee Yong Hee Jeon Sun Lim Kim Gun-Ho Jung Beom-Young Son Koan Sik Woo 《Journal of Crop Science and Biotechnology》2018,21(1):57-65
We evaluated the proximate composition, free sugar content, fatty acid composition, carotenoid content, total phenol content, and radical scavenging activity of the grain from various Korean maize hybrid cultivars grown in two different cropping seasons. The moisture, crude ash, crude fat, crude protein, total starch, and amylose contents were significantly higher in most of the maize hybrid cultivars when grown in the early-season than when grown in the late-season. The free sugar content, fatty acid composition, carotenoid content, and total phenol content differed significantly between cultivars and cropping seasons. The highest unsaturated fatty acid compositions of maize hybrids of early-season and late-season were 86.05 and 86.29%, respectively, in the Daanok cultivar. The carotenoid contents were significantly higher in maize hybrids of late-season compared to those of early-season. The highest total phenol content was 108.09 mg/100 g in Singwangok of the late-season. The radical scavenging activity of maize hybrids differed significantly between cultivars and cropping seasons. 相似文献
62.
Haejeen Bang Dong Youn Cho Kil-Sun Yoo Moo-Kyoung Yoon Bhimanagouda S. Patil Sunggil Kim 《Euphytica》2011,179(3):439-449
In order to implement reliable marker-assisted selection systems for the restorer-of-fertility locus (Ms) in onions (Allium cepa L.), simple PCR-based codominant markers linked to the Ms locus were developed. Based on the EST probe sequences of previously reported RFLP markers, full-length genomic sequences
of the gene encoding putative oligopeptide transporter (OPT) was obtained by RACE. The first intron contained two 108 and
439-bp indel polymorphisms between the two Ms allele-linked OPT alleles. A simple PCR marker for OPT was developed by designing a primer pair on the flanking regions of
the 108-bp indel which is created by two tandem repeats. The second simple PCR marker was developed from the EST probe encoding
photosystem I subunit O (PsaO). Two 14 and 39-bp tandem repeats were identified from the 5′ upstream sequences of the PsaO-coding
gene, which were isolated by genome walking. Three different compositions of these tandem repeats were identified from diverse
onion germplasm. A primer set binding to the flanking sequence of these polymorphic repeats was used to amplify three different
marker haplotypes. The OPT marker was tightly linked to the Ms locus at a distance of 1.5 cM, but the analysis of the linkage relationship showed little linkage disequilibrium between
the marker and the Ms locus. Even so, these simple PCR markers are valuable tools for the marker-assisted selection of segregating individuals
in onion F1 hybrid breeding programs. 相似文献
63.
Genetic variation and relationships among members of the azuki bean complex (Vigna angularis) including wild (V. angularis var. nipponensis), weedy, and cultivated types (V. angularis var. angularis), V. nakashimae, and rice bean (V. umbellata) from Korea were examined using the Amplified fragment length polymorphism (AFLP) method. AFLP analysis of 50 accessions
revealed 333 (72.1%) polymorphic fragments out of 462 fragments amplified using seven primer combinations. The number of polymorphic
fragments within each species was 70 in the azuki bean complex and 41 in V. nakashimae, but there was no polymorphism in rice bean. The number of shared fragments among species ranges from 142 between the azuki
bean complex and V. nakashimae to 166 between the azuki bean complex and rice bean. Within the azuki bean complex, the range of shared bands was from 231
between cultivated and weedy types to 238 between cultivated and wild types. A dendrogram generated from Jaccard’s similarity
matrix was divided into three groups, which correspond to V. nakashimae, azuki bean complex, and rice bean. The relationship between azuki bean and rice bean is closer than between azuki bean and
V. nakashimae. Phenetic distances averaged 0.502 between the azuki bean complex and V. nakashimae and 0.467 between the azuki bean complex and rice bean. Within the azuki bean complex, the weedy type was more closely related
to wild than cultivated types. But UPGMA dendrogram of the azuki bean complex reveals that each type is not clearly isolated.
These results will help to understand genetic diversity and evolutionary dynamics of Vigna in Korea. 相似文献
64.
Jundae Lee Jae Bok Yoon Jung-Heon Han Won Phil Lee Sang Hoon Kim Hyo Guen Park 《Euphytica》2010,173(1):55-61
Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms
3
gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP)
techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc276, Eagc/Mctt178, and Ecag/Mtgc204) were identified as tightly linked to the ms
3
locus. Among them, we converted the AFLP marker Ecag/Mtgc204 to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and
flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using
the GMS system. 相似文献
65.
QTL mapping for capsaicin and dihydrocapsaicin content in a population of Capsicum annuum ‘NB1’ × Capsicum chinense ‘Bhut Jolokia’ 下载免费PDF全文
Jundae Lee Seok Jin Park Sun Chul Hong Jung‐Heon Han Doil Choi Jae Bok Yoon 《Plant Breeding》2016,135(3):376-383
Capsaicinoids are pungent compounds used for industrial and medical purposes including food, medicine and cosmetics. The Indian local variety ‘Bhut Jolokia’ (Capsicum chinense Jacq.) is one of the world's hottest chilli peppers. It produces more than one million Scoville heat units (SHUs) in total capsaicinoids. In this study, our goal was to identify quantitative trait loci (QTLs) responsible for the high content of capsaicin and dihydrocapsaicin in ‘Bhut Jolokia’. Capsicum annuum ‘NB1’, a Korean pepper inbred line containing 14 000 SHUs, was used as a maternal line. An F2 population derived by crossing between ‘NB1’ and ‘Bhut Jolokia’ was generated to map QTLs for capsaicinoids content. A total of 234 markers, including 201 HRM, 21 SSR, 2 CAPS and 10 gene‐based markers of the capsaicinoid synthesis pathway, were mapped. The final map covered a total distance of 1175.2 cM and contained 12 linkage groups corresponding to the basic chromosome number of chilli pepper. Capsaicin and dihydrocapsaicin content were analysed in 175 F2 pepper fruits using the HPLC method. The maximum total capsaicinoids content was 1389 mg per 100g DW (dry weight), and the minimum content was 11 mg per 100g DW. Two QTLs (qcap3.1 and qcap6.1) for capsaicin content were identified on LG3 and LG6, and two QTLs (qhdc2.1 and qdhc2.2) for dihydrocapsaicin content were located on LG2. We did not detect QTLs for total capsaicinoids content. The QTL positions for capsaicin content were different from those for dihydrocapsaicin content. These results indicate that the complexity of selecting for more pungent chilli peppers must be considered in a chilli pepper breeding programme. The QTL‐linked markers identified here will be helpful to develop more pungent pepper varieties from ‘Bhut Jolokia’, a very hot pepper. 相似文献
66.
Hee Ock Boo Jeong Hun Park Hag Hyun Kim Soo Jeong Kwon Sun Hee Woo 《Journal of Crop Science and Biotechnology》2018,21(5):507-514
This study was conducted to investigate cytotoxic effect, phenolic content, DPPH, ABTS radical scavenging rate and nitrite scavenging rate from different solvent fractions of Codonopsis lanceolate root. At all extracts concentration, the cytotoxic effect on different fractions against human cancer cells was higher in n-hexane and butyl alcohol fractions than in the other fractions. The IC50 value on HeLa cell showed the lowest by 62.70 μg.mL-1 on n-hexane fraction, and exhibited the values of butyl alcohol fraction 341.36 μg.mL-1, methylene chloride 598.33 μg.mL-1, ethyl acetate fraction 860.44 μg.mL-1, DW fraction 2896.82 μg.mL-1. Total polyphenol content on different solvent fractions varied from 102.43 to 153.52 mg.g-1, and that of ethyl acetate fraction showed the highest amount. The DPPH and ABTS radical scavenging activity showed that the increase was proportional to the concentration, and the scavenging activity also showed the highest in ethyl acetate fraction. The nitrite scavenging activity of each fraction at pH 1.2 was in the order of EA > BA > MC > n-H > DW, and the lower the acidity, the higher nitrite scavenging activity, and there was no distinct detection of nitrite scavenging effects of the pH range 6.0. The results of this study suggested that the extract of Codonopsis lanceolate root may assist in the potential biological activities, and it was found that the activity was different depending on the organic solvent fraction and the water fraction. 相似文献
67.
Methyl jasmonate alleviated salinity stress in soybean 总被引:1,自引:0,他引:1
Ji Young Yoon Muhammad Hamayun Su-Kyung Lee In-Jung Lee 《Journal of Crop Science and Biotechnology》2009,12(2):63-68
We studied the role of methyl jasmonate (MeJA) in alleviating NaCl-induced salt stress on soybean growth and development in
hydroponics medium. Soybean seedlings were exposed to 60 mM NaCl stress for 2 weeks, 24 h after the application of 20 and
30 μM MeJA. NaCl stress induced a significant reduction in plant growth, endogenous bioactive gibberellin (GA4), photosynthesis
and transpiration rate, while a marked increase in the endogenous abscisic acid (ABA) and proline contents were recorded.
MeJA application greatly mitigated the adverse effects of NaCl on soybean growth and endogenous hormones. MeJA significantly
increased ABA levels, while the endogenous amount of GA4 was reduced by the application of NaCl. Our study revealed that MeJA
counteracted the negative effects of NaCl stress on plant growth, chlorophyll content, leaf photosynthetic rate, leaf transpiration
rate, and proline content. 相似文献
68.
Mun Sup Yoon Jeongran Lee Chang Yung Kim Jung Hoon Kang Eun Gi Cho Hyung Jin Baek 《Euphytica》2009,165(1):69-77
Approximately 7,000 accessions of Korean soybean (Glycine max (L.) Merrill) landraces, largely composed of three collections, the Korea Atomic Energy Research Institute’s soybean (KAS),
the Korean Crop Experiment Station’s soybean (KLS) and the Korean Agricultural Development and Technology Center’s soybean
(KADTC) collections, have been conserved at the Rural Development Administration (RDA) genebank in Korea. The accessions within
collections were classified based on their traditional uses such as sauce soybean (SA), sprouted soybean (SP), soybean for
cooking with rice (SCR), and OTHERS. A total of 2,758 accessions of Korean soybean landraces were used to profile and to evaluate
genetic structure using six SSR loci. A total of 110 alleles were revealed by at the six SSR loci. The number of alleles per
SSR locus ranged from 9 to 39 in Satt187 and Satt_074, respectively. The number of alleles ranged from 87 in the KADTC collection
to 96 in the KLS collection, and from 63 in the SCR group to 95 in the SP group. Nei’s average genetic diversity ranged from
0.68 to 0.70 across three collections, and 0.64 to 0.69 across the usage groups. The average between-group differentiation
(G
st) was 0.9 among collections, and 4.1 among the usage groups. The similar average diversity among three collections implies
that the genetic background of the three collections was quite similar or that there were a large number of duplicate accessions
in three collections. The selection from the four groups classified based upon usage may be a useful way to select accessions
for developing a Korean soybean landrace core collection at the RDA genebank. DNA profile information of accessions will provide
indications of redundancies or omissions and aid in managing the soybean collection held at the RDA genebank. The information
on diversity analysis could help to enlarge the genetic diversity of materials in breeding programs and could be used to develop
a core collection. 相似文献
69.
Woo Duck Seo Jun Young Kim You-Chun Song Jun-Hyun Cho Ki Chang Jang Sang-Ik Han Ji-Eun Ra Seong-Hwan Oh Hyeon-Jung Kang Byung-Joo Kim Nam-In Baek Rak-Hun Jeong Min Hee Nam 《Journal of Crop Science and Biotechnology》2013,16(1):63-68
The main objectives of this study were to investigate physicochemicals and antioxidant activities of new red rice (Oryza sativa cv. Gunganghongmi (GH)) by comparing normal brown (Nampyeongbyeo, NB) and reported red rice (Jukjinjubyeo, JB) in Korea. The nutritional constituents, including protein, oil, sugar, fatty acid, GABA, and γ-oryzanol were not significantly different between normal brown and colored rice. However, the ethanol extract of GH showed the highest phenolic content (24.7 ± 1.3 mg g?1). The ethanol extracts of GH showed higher scavenging activities against DPPH (0.2 mg mL?1 = 62.1 ± 2.5%) and ABTS (0.2 mg mL?1 = 63.2 ± 3.5%) radicals. Moreover, GH more inhibited LPS-induced nitric oxide (NO) production (13.2 ± 1.4 μM) than JB (18.3 ± 2.3 μM) and NB (22.1 ± 1.4 μM) at the same concentration (0.2 mg mL?1) without cytotoxicity. These results suggest that new red rice (GH) would be considered to be new functional rice due to its anti-oxidative effect and high nutrition. 相似文献
70.