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61.
Naturally occurring and experimental Stachys arvensis (staggerweed) toxicity in lambs in New Zealand
Stachys arvensis (staggerweed) is a common, widely distributed weed of cultivated and waste land with the potential to intoxicate sheep. Two naturally occurring outbreaks of suspected staggerweed toxicity in the lower North Island were investigated. Affected lambs had been recently moved onto staggerweed-contaminated Brassica spp. crops. In total, 150/1,200 (13%) lambs developed hindlimb paresis, a fine generalised muscular tremor, and hunched posture. When forced to move, many became recumbent. Most lambs recovered within 48 h of removal from staggerweed, although a few developed clinical signs again when transported 2–3 weeks later. Grossly, affected lambs had large amounts of staggerweed plant material and seeds within the rumen. Histopathology showed mild, multifocal degeneration of the white matter tracts of the central nervous system (CNS), most commonly in the ventral funiculi of the spinal cord, and acute, mild to moderate, multifocal degeneration of skeletal muscles. Creatine kinase (CK) activity in serum was mildly to markedly elevated in affected lambs. In a feeding trial, ten 10-month-old Romney lambs were randomly assigned to equal treatment and control groups. Treated lambs were drenched with a liquid extract of staggerweed once daily for 7 days. Three of five treated lambs developed mild exercise intolerance, and 1/5 displayed mild paresis of the hindlimbs, slightly crouched hindlimb stance, and shortened gait, on days 6 and 7. Histologically, 4/5 treated lambs had degeneration in white matter tracts of the CNS, indistinguishable from those seen in the lambs in the outbreak, and in 1/5 lambs there was scattered regeneration of skeletal muscle. CK activity in serum in treated lambs was not significantly higher than that in control lambs. None of the control lambs developed significant clinical signs, histological changes or increases in CK activity in serum. The clinical signs and lesions observed in both the outbreaks and feeding trial were similar to those previously described in studies in Australia, with the exception that myodegeneration was more prominent in the outbreaks in New Zealand. Further characterisation of the pathogenesis of staggerweed toxicity and its potential role as a food safety hazard will be facilitated through identification of the toxic principle(s). 相似文献
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The foresters' perspectives of agroforestry is an important aspect that can influence strategies concerned with agroforestry uptake by the farmers. Keeping this in view the empirical studies of foresters' perspective of agroforestry have gained considerable importance. Present study, therefore, investigates foresters' perspectives on different aspects related to agroforestry. This study was conducted in Mandi district located in Indian Western Himalayan province of Himachal Pradesh. Not only the traditional forest management but also agroforestry training of foresters has remained oriented towards learning silvi-technical (plantation management and nursery management) rather than extension (social) and agricultural aspects. Consequently, there was a felt need amongst foresters for training in extension and agricultural aspects for effective implementation of agroforestry programmes. This provides a window of opportunity for re-orienting their training curriculum towards learning extension (social) and agricultural besides the silvi-technical skills. For effective implementation of agroforestry programmes the manpower requirement, particularly that of extension specialist and well trained agroforesters, of the department needs to be estimated and strategy should be devised to minimise political interference. The study emphasises a need for interaction between foresters and farmers, mechanism for better co-ordination with other departments and absolving agroforesters of their policing role for effective planning and implementation of agroforestry programmes. There were significant variations in the opinion of forestry staff about the effectiveness of various strategies to meet farmers tree-based needs, increase tree cover in the state and expected plantation survival rate. There were significant variations in the opinion of foresters about the different factors discouraging private tree growing but ‘restriction on felling green trees growing on private land and selling them in the market’ was viewed as the most important factor. “Provision of incentives to the villagers for tree growing on private land” was the major factor from the foresters' perspective that will encourage tree growing on private land. As there was a lack of congruence of foresters' perception on majority of issues pertaining to agroforestry, a need to incorporate the uniform understanding of issues pertaining to agroforestry amongst the foresters was emphasised. This study also identifies a need to investigate the perspectives staff of agriculture, horticulture departments for better planning and execution of agroforestry programmes, and their uptake by the farmers. 相似文献
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Summary
Ipomoea cairica
Sweet (Convolvulaceae) exhibits sporophytic self-incompatibility. This has partially been overcome under in vitro conditions, by treating the pollen and/or stigma with 10-5–10-1M indole-3-acetic acid (IAA), the optimum being 10-2M. The self-pollen. which otherwise does not even stick to stigma. germinates after self-pollination provided only one or both the partners are treated. The pollen tubes not only penetrate stigmatic papillae but also traverse the whole length of the style, at least in optimum experimental combinations. 相似文献
67.
P. K. Pradhan J. K. Jena G. Mitra N. Sood E. Gisbert 《Fish physiology and biochemistry》2012,38(6):1601-1617
The ontogeny of the digestive tract was studied histologically in butter catfish Ompok bimaculatus from hatching to 30?days post-hatching (dph). At hatching, the digestive tract of butter catfish consisted of a straight tube with a smooth lumen dorsally attached to the yolk sac. Between 1 and 2 dph, the mouth opened, oral valves were visible and canine-like teeth and taste buds were detected. During this period, intestine was differentiated into the anterior and posterior intestine, and the digestive accessory glands were also developed. Exogenous feeding started at 2?dph, and there was a 2-day mixed endogenous–exogenous feeding period. Most of the yolk sac reserves were consumed between 2 and 3 dph, and by 5?dph, the yolk sac was completely depleted and no longer visible in histological sections. Between 3 and 4 dph, several vacuoles (neutral lipids) were observed in the intestine and also in hepatocytes, indicating a functional absorption of nutrients from food. At 8 dph, differentiation of gastric glands was noticed, and by 9–11?dph, there were abundant gastric tubular glands arranged along numerous longitudinal folds. During the same period, pyloric sphincter appeared as an epithelial fold that separated the stomach from the anterior intestine. From 12 dph to the end of the study at 30?dph, no noticeable histological modifications were observed. The development of gastric glands is considered as the last major events in digestive tract development and their presence designates the end of larval period and the onset of the juvenile period. Hence, it is suggested that, butter catfish larvae have a morphologically complete digestive tract by 12?dph. These findings on the development of the digestive system in butter catfish may lead to a better understanding of the ontogeny and would be useful to improve the larval rearing techniques of this promising catfish species for freshwater aquaculture diversification. 相似文献
68.
Endometrial gene expression in primiparous dairy cows at the end of the voluntary waiting period is affected by nutrition: Total mixed ration vs increasing levels of herbage allowance 下载免费PDF全文
AL Astessiano M Carriquiry DA Mattiauda ML Adrien P Chilibroste A Meikle 《Reproduction in domestic animals》2017,52(5):798-805
The study postulated that differential nutritional management during the early lactation period would be reflected in endometrial expression of genes related to embryo growth at the end of the voluntary waiting period. Thus, the effect of the combined use of total mixed ration (TMR) and grazing under different herbage allowances during the first 75 days post‐partum (DPP) on endometrial gene expression was evaluated in primiparous dairy cows. Cows were blocked by body weight, age and body condition score and randomly assigned to three grazing treatments: high (HA, 30 kg DM per cow per day), medium (MA, 15 kg DM per cow per day) and low (LA, 7.5 kg DM per cow per day) herbage allowance (mixed pasture, 2,600 kg DM per ha) plus 8 kg DM of supplement or TMR (55% forage, 45% concentrate) fed ad libitum (TMR) from calving to 75 DPP. At 57 DPP, cows were synchronized for oestrus (day 0, 68 DPP) and at day 7, endometrial biopsies were obtained. The nutritional treatment did not affect insulin, IGF‐1 and leptin concentrations on days 0, 4 or 7. Expression of IGF1, IGFBP3, IGFBP4, ADIPOR1 and ADIPOR2 mRNA was significantly affected by the nutritional treatment. Endometrial IGF1 and IGFBP4 mRNA were twofold greater in TMR and HA than MA and LA cows. Expression of IGFBP3 and ADIPOR1 mRNAs was greater in TMR and HA than MA cows, but did not differ from LA cows. All groups had greater expression of ADIPOR2 mRNA than MA cows. This study provided solid evidence of the importance of nutritional management during early lactation on uterine environment at the end of the voluntary waiting period. The greater expression of genes related to embryo growth and uterine function (IGF system, progesterone and adiponectin receptors) in cows fed diets maximizing energy intake suggests a favourable environment for embryonic growth, which may explain the improved reproductive performance of cows in good energy balance. 相似文献
69.
Sensitivity of boreal forest carbon balance to soil thaw 总被引:6,自引:0,他引:6
ML Goulden SC Wofsy JW Harden SE Trumbore PM Crill ST Gower T Fries BC Daube S Fan DJ Sutton A Bazzaz JW Munger 《Science (New York, N.Y.)》1998,279(5348):214-217
We used eddy covariance; gas-exchange chambers; radiocarbon analysis; wood, moss, and soil inventories; and laboratory incubations to measure the carbon balance of a 120-year-old black spruce forest in Manitoba, Canada. The site lost 0.3 +/- 0.5 metric ton of carbon per hectare per year (ton C ha-1 year-1) from 1994 to 1997, with a gain of 0.6 +/- 0.2 ton C ha-1 year-1 in moss and wood offset by a loss of 0.8 +/- 0.5 ton C ha-1 year-1 from the soil. The soil remained frozen most of the year, and the decomposition of organic matter in the soil increased 10-fold upon thawing. The stability of the soil carbon pool ( approximately 150 tons C ha-1) appears sensitive to the depth and duration of thaw, and climatic changes that promote thaw are likely to cause a net efflux of carbon dioxide from the site. 相似文献
70.
Anthelmintic resistance in Australian sheep nematode populations 总被引:2,自引:0,他引:2
SUMMARY The resistance status of gastro–intestinal nematodes to anthelmintics was evaluated on 881 sheep farms throughout Australia during 1991–92. Resistance was shown to be widespread. Overall, 85% of farms had sheep infected with nematodes resistant to benzimidazole, 65% to levamisole and 34% to combination (benzimidazole + levamisole) products. Resistance to ivermectin was not detcted. On only 9% of farms did all anthelmintic groups reduce egg counts by greater than or equal to 95%. The culture of faeces from untreated sheep showed Telodorsagia circumcincta, Trichostrongylus spp, Chabertia ovina and Haemonchus contortus to be the principal species. The nature and prevalence of resistance was not significantly correlated with stocking rate. However, resistance to combination products was almost twice as prevalent on farms in areas with an average annual rainfall of greater than 500 mm. 相似文献