Effect of Luteolin on Blood Indexes,Liver and Kidney in Mice with Acute Mercury Poisoning

To study the effect of luteolin on blood indexes,liver and kidney in mice with acute mercury poisoning,28 mice were randomly divided into four groups:Control group (intraperitoneal injection 0.9% saline),luteolin group (lavage 100 mg/kg luteolin),mercuric chloride group (intraperitoneal injection 4 mg/kg mercury chloride) and mercury chloride+luteolin group (intraperitoneal injection 4 mg/kg mercuric chloride,lavage 100 mg/kg luteolin).The activities of ALT in serum,AST,CREA and BUN contents,blood WBC,RBC,HGB content and GSH and MDA contents of liver tissue were detected.Morphological changes of liver and kidney tissues were observed.The results showed that compared with the control group,the activities of ALT and AST of mercuric chloride group were extremely significantly increased (P < 0.01),serum CREA,BUN,blood WBC and liver tissue MDA contents were significantly increased (P < 0.05) while blood RBC,HGB and liver tissue GSH contents were significantly decreased (P < 0.05).Liver,kidney pathological changes were obviously.Compared with mercuric chloride group,the activities of ALT,AST in serum,CREA,BUN,blood WBC and liver tissue MDA contents of mercuric chloride+luteolin group were significantly decreased (P < 0.05),while blood RBC,HGB and liver tissue GSH contents were significantly elevated (P < 0.05).Liver,kidney pathological changes were attenuated obviously.The poisoning were characterized by inflammation and the occurrence of anemia when acute mercury poisoning occurred,liver and kidney showed different degrees of injury in mice.Luteolin could reduce the toxic effects of acute mercury poisoning on blood,liver and kidney.     

不同来源硒对异育银鲫的生物学效应研究

以异育银鲫为试验动物,研究不同来源的硒(蛋氨酸硒和纳米硒)对其生长性能、肌肉生化组成和谷胱甘肽过氧化物酶活性的影响。试验分为3个处理,日粮中添加硒浓度分别为0 mg/kg(对照组)、0.5 mg/kg(蛋氨酸硒)和0.5 mg/kg(纳米硒),每个处理3个重复。结果显示,与对照组相比,蛋氨酸硒和纳米硒均可显著提高异育银鲫的末重、相对增重率以及谷胱甘肽过氧化物酶活性(P<0.05),但是蛋氨酸硒处理组和纳米硒处理组间差异不显著。纳米硒处理组肌肉中硒含量显著高于其他两组(P<0.05),蛋氨酸硒处理组肌肉中硒含量显著高于对照组(P<0.05)。研究表明,饵料中添加一定浓度的蛋氨酸硒和纳米硒具有一定的生物学效应。     

Seroprevalence of chlamydial infection in dairy cattle in Guangzhou, southern China

Chlamydia spp. are obligate intracellular gram-negative bacteria that cause a wide range of significant diseases in humans and animals worldwide, resulting in significant economic losses. Chlamydial infection in cattle has been reported in many countries including China. However, there has been no survey of chlamydial infection of dairy cattle in Guangzhou, southern China. The objective of the present investigation was to examine the chlamydial seroprevalence in dairy cattle in Guangzhou, subtropical southern China by using an indirect hemagglutination assay (IHA). The overall seroprevalence of chlamydial infection in dairy cattle was 7.25% (29/400). Greater than or equal to eight-yr-old dairy cattle had the highest seroprevalence (10.34%), followed by those that were ≥ 6 years old or < 7 years old dairy cattle (10.20%), although there were no statistically significant differences among different groups (P > 0.05). Dairy cattle with 5 pregnancies had the highest seroprevalence (10.81%). These results indicate that chlamydial infection was present in dairy cattle in Guangzhou, subtropical southern China, and integrated strategies and measures should be executed to control and prevent chlamydial infection and disease outbreak in the study region.     

乳及乳制品中三聚氰胺检测方法的研究进展

本文综述乳及乳制品中三聚氰胺的分析检测方法,对其优缺点进行分析比较,并对前处理过程中出现的问题进行讨论.     

分娩前饲喂包被氯化钙对母猪产程和死胎的影响

本试验旨在研究分娩前饲喂包被氯化钙对母猪产程和死胎的影响。选取913头妊娠109 d丹系长×大和大×长二元杂交母猪,根据体况和胎次接近的原则分成3组。对照组于妊娠109 d至分娩当天每天饲喂基础饲粮,试验Ⅰ组和试验Ⅱ组在基础饲粮的基础上分别额外饲喂25和50 g/d包被氯化钙。饲养试验从妊娠109 d到分娩当天。试验过程中每天进行粪便评分,于分娩当天记录试验母猪产程、产仔数和死胎数,并采集尿液和血液分别用于测定尿液pH和血清钙离子浓度。结果表明:1)总产仔数和分娩前饲喂包被氯化钙对母猪死胎数无显著交互作用(P>0.05);试验Ⅰ组和试验Ⅱ组死胎数分别为0.31和0.28头,显著低于对照组的0.44头(P<0.05),但试验Ⅰ组和试验Ⅱ组无显著差异(P>0.05)。2)试验Ⅰ组母猪产程相较于对照组显著缩短(P<0.05),而试验Ⅱ组产程相较于对照组无显著差异(P>0.05);3组母猪胎盘排出时间之间无显著差异(P>0.05)。3)3组母猪妊娠109 d和分娩当天尿液pH之间无显著差异(P>0.05);妊娠110 d到妊娠113 d,对照组和试验Ⅰ组尿液pH无显著差异(P>0.05),试验Ⅱ组尿液pH显著低于对照组和试验Ⅰ组(P<0.05)。与对照组相比,试验组母猪血清钙离子浓度显著提高(P<0.05),妊娠112 d和分娩当天粪便评分显著降低(P<0.05);3组母猪便秘率之间无显著差异(P>0.05)。综上所述,母猪妊娠109 d至分娩前饲喂25 g/d包被氯化钙可显著缩短产程,减少死胎,而且该效果不受母猪总产仔数的影响,该调控效果与其提高血液钙离子浓度有关。     

2017年北京市猪伪狂犬病血清学调查

为了解北京市猪伪狂犬病野毒感染情况,2017年从北京市7个区157个养猪场（户）,采集5 469份猪血清样品,采用猪伪狂犬病病毒（PRV）gE抗体酶联免疫检测试剂盒进行血清学调查。结果显示：北京市7个区均有不同程度的PRV野毒感染,平均样品阳性率为53.36%。其中：经产母猪阳性率最高（58.28%）,其次是育成猪（57.55%）、育肥猪（54.49%）,后备母猪阳性率最低（37.46%）;散养户的样品阳性率较高,为66.42%,规模猪场的阳性率较低,为47.71%。调查结果表明,北京市PRV野毒感染较为严重,对猪场采取伪狂犬病净化和综合防控措施势在必行。     

PERT网络模型在家禽规模养殖项目管理中的运用

本文针对农业科技专家大院草科鸡规模养殖项目运行和管理中存在的问题,就优化农业科技项目管理与提高项目运行的效用和功能,提出采用PERT网络模型管理农业科技项目的思路,并对项目管理过程、方法及注意事项等问题提出了建议。本文指出,运用科学的管理方法制定合理的项目运行计划,是进行科技项目组织管理、科学地配置资源和促进科研项目顺利完成的重要途径。     

短穗兔耳草的克隆生长特征

以调查统计的方法研究了高寒草甸匍匐茎植物短穗兔耳草Lagotis brachystachya无性系的生长特征、形态特征以及能量分配规律,研究结果表明:匍匐茎只有1条的短穗兔耳草最多,占60．47％,匍匐茎有4条的短穗兔耳草只占6．98％。在牧草生长盛期,短穗兔耳草无性系的基株高度4．03 cm,根长为9．11 cm,叶数为7．98;分株高度0．85 cm,根长2．73 cm,叶数为3．08;匍匐茎的茎生叶数为12．54,长度为15.14 cm,匍匐茎比节间重为2．18 mg／cm,粗度为0．97 mm,不同构件的干质量呈现出基株>匍匐茎>分株>茎生叶的规律。随着匍匐茎数目的增多,短穗兔耳萆无性系的基株根长逐渐增加,用于克隆繁殖的能量投资也有增加的趋势,其他参数都没有明显的变化规律。     

不同干燥方法对苜蓿相同区段营养价值影响的研究

取同一地块现蕾期紫花苜蓿,分成上1/3、下2/3和全株苜蓿3部分样品,每种样品都分别采用自然通风晒干、室内通风阴干和60～65℃人工烘干3种不同的方式进行干燥。每个样品都进行干物质、粗蛋白、粗脂肪、粗纤维等营养成分的测定,结果表明:粗蛋白含量以晒干方式的最高(P<0.05);苜蓿干草中粗脂肪类物质的含量受干燥中温度的影响较大,随温度升高粗脂肪含量有增大的趋势;苜蓿不同的区段粗纤维含量均以风干干燥方式的最高(P<0.05)。     

凋亡素基因真核表达载体的构建及在人肿瘤细胞中的表达

为探讨凋亡素对肿瘤的基因治疗效果,构建了凋亡素基因的真核表达载体。将凋亡素基因重组入 ｐｃ Ｄ Ｎ Ａ３ 载体,并通过脂质体介导凋亡素在人喉癌、人肺癌细胞系中表达;通过 Ｒ Ｔ Ｐ Ｃ Ｒ 在转染细胞中检测到了凋亡素的 ｍ Ｒ Ｎ Ａ,这为应用凋亡素进行肿瘤的基因治疗奠定了基础。