M gene evolution of canine coronavirus in naturally infected dogs

Two stray pups (A and B), three and five months old, respectively, both naturally infected with canine coronavirus (CCoV), were studied for 180 days. The virus was detected intermittently in the pups' faeces by PCR for periods of 156 and 146 days, respectively. Sequence analysis of a fragment of the gene encoding the M protein revealed that the viruses detected at the onset of the infection were very similar to typical strains of CCoV, whereas from 42 days after infection in pup A and 40 days after infection in pup B the viruses had nucleotide and amino acid mutations resembling sequences in feline coronavirus.     

Phenological and pomological properties of promising walnut (Juglans regia L.) genotype with cluster fruiting from selected native population in Oltenia,Romania

Genetic Resources and Crop Evolution - Lateral fruiting in walnut is an important feature and many breeding programs follow this criterion. Following the evaluation of walnut genotypes from Padea...     

中美农产品贸易对中国农业发展影响的实证分析

根据1993—2011年中美两国农产品贸易总额和中国农业总产值的数据,采用协整分析、Granger因果关系检验和误差修正模型对中美农产品贸易与中国农业发展的关系进行了实证分析。结果表明,中美农产品贸易与中国农业发展存在长期的均衡关系;中美农产品贸易总额是中国农业发展的Granger原因;从短期来看,当短期波动偏离长期均衡时,误差修正项以0.371的力度作反向修正,将非均衡状态恢复到均衡状态。     

中美农业技术进步贡献率比较分析与展望

作为衡量经济增长方式的主要方法,全要素生产率对经济增长贡献大小的核算已成为学界的研究热点,与之相关的实证方法有增长核算法、随机前沿法和DEA-Malmquist生产率指数法。利用3种实证方法和FAO数据库中1980—2007年中美农业相关数据,考察了中关农业技术进步情况,通过对技术进步贡献率的比较分析,找出中关农业技术进步的差距,为中国农业发展指明方向。     

Assembly of feline calicivirus-like particle and its immunogenicity

Virus-like particles (VLPs) were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of feline calicivirus strain F9 (FCV-F9). The FCV VLPs were morphologically and antigenically similar to the native virus and contained a single capsid protein with a molecular weight of approximately 60 kDa that reacted with FCV antiserum. Moreover, following immunization of rabbits, VLPs were able to elicit neutralizing antibodies against several FCV strains isolated from clinical samples. Our preliminary results showed that FCV-VLP could be considered a candidate vaccine against FCV infections.