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101.
Assessment of method reliability and determination of reference intervals for rotational thromboelastometry in horses
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102.
103.
Oluwaseun A. Mustapha Matthew A. Olude Samuel Ezekiel Johannes Seeger Simone A. Fietz James O. Olopade 《Anatomia, histologia, embryologia》2019,48(5):486-497
The Greater cane rat (GCR, Thyronomys swinderianus) is a precocial rodent predominantly found within Africa. Economic and scientific interests have led to several research efforts towards the domestication and better understanding of the biology and development of this rodent. Despite these efforts, information on the pre‐natal development of this rodent is currently lacking. This study characterises distinct developmental milestones including skin pigmentation, emergence and distributions of hairs, calvarium consistency, teeth eruption, development of appendages, sensory organs and external genitalia in the pre‐natal GCR and assesses quantitative body parameters, that is body weight, body and crown–rump lengths across its entire gestation length (gestation days [GDs] 10‐140). Using these external features, we provide baseline reference ontogenetic scales for GCR embryos and fetuses, employable for stage, age and sex estimation of the pre‐natal GCR in future studies. We observed that the first evidence of an embryo was not seen before the end of the first trimester (GD50) and that the late second trimester (GD80‐GD100) marks the transition from embryogenesis to fetogenesis in the GCR. As both events occur at a much later developmental time point when compared to precocial non‐rodents including human, sheep and pig and slightly later when compared to other precocial rodents such as guinea pig, our data provide first indication that the pre‐natal GCR development might be associated with a reproductive delay. Together, this study expands our knowledge of the development and biology of the GCR, which will improve reproductive and breeding management, and native species conservation of this hystricomorph mammal. 相似文献
104.
Denise S. Okano Jurandy M. Penitente‐Filho Victor E. Gomez Len Paula P. Maitan Camila O. Silveira Bruna Waddington Edgar A. Díaz‐Miranda Eduardo P. da Costa Simone E. F. Guimares Jos D. Guimares 《Reproduction in domestic animals》2019,54(3):604-612
This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed‐time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analysed in the laboratory after thawing. Sperm motility and vigour were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H2O2 production were evaluated by flow cytometer. Relation among sperm characteristics and pregnancy rate of cows and heifers were evaluated by univariate and multivariate logistic regression. Subjective sperm motility and vigour did not affect the probability of pregnancy in cows or heifers. In univariate analysis for pregnancy in cows, sperm traits related to acrosome injury positively affected probability of pregnancy mainly when associated with plasma membrane integrity; H2O2 production seems to be less important than plasma membrane integrity in affecting probability of pregnancy. In multivariate analysis, sperm traits related to injured acrosome positively affected probability of cow and heifer pregnancies while intact acrosome was negatively related to cow pregnancy. Intact plasma membrane and high H2O2 production were positively related to cow pregnancy but negatively related to heifer pregnancy. Results suggest that a capacitation‐like status of the acrosome may benefit probability of pregnancy in cows. 相似文献
105.
High dose hypofractionated frameless volumetric modulated arc radiotherapy is a feasible method for treating canine trigeminal nerve sheath tumors
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106.
107.
Carolina Meira Fabiola B. Joerger Annette P.N. Kutter Andreas Waldmann Simone K. Ringer Stephan H. Böehm Samuel Iff Martina Mosing 《Veterinary anaesthesia and analgesia》2018,45(2):145-157
Objective
To compare the efficacy of three continuous positive airway pressure (CPAP) interfaces in dogs on gas exchange, lung volumes, amount of leak during CPAP and rebreathing in case of equipment failure or disconnection.Study design
Randomized, prospective, crossover, experimental trial.Animals
Ten purpose-bred Beagle dogs.Methods
Dogs were in dorsal recumbency during medetomidine–propofol constant rate infusions, breathing room air. Three interfaces were tested in each dog in a consecutive random order: custom-made mask (M), conical face mask (FM) and helmet (H). End-expiratory lung impedance (EELI) measured by electrical impedance tomography was assessed with no interface (baseline), with the interface only (No-CPAP for 3 minutes) and at 15 minutes of 7 cmH2O CPAP (CPAP-delivery). PaO2 was assessed at No-CPAP and CPAP-delivery, partial pressure of inspired carbon dioxide (PICO2; rebreathing assessment) at No-CPAP and the interface leak (ΔPleak) at CPAP-delivery. Mixed-effects linear regression models were used for statistical analysis (p < 0.05).Results
During CPAP-delivery, all interfaces increased EELI by 7% (p < 0.001). Higher ΔPleak was observed with M and H (9 cmH2O) in comparison with FM (1 cmH2O) (p < 0.001). At No-CPAP, less rebreathing occurred with M (0.5 kPa, 4 mmHg) than with FM (1.8 kPa, 14 mmHg) and with H (1.4 kPa, 11 mmHg), but also lower PaO2 was measured with M (9.3 kPa, 70 mmHg) than with H (11.9 kPa, 90 mmHg) and FM (10.8 kPa, 81 mmHg).Conclusions and clinical relevance
All three interfaces can be used to provide adequate CPAP in dogs. The leak during CPAP-delivery and the risk of rebreathing and hypoxaemia, when CPAP is not maintained, can be significant. Therefore, animals should always be supervised during administration of CPAP with any of the three interfaces. The performance of the custom-made M was not superior to the other interfaces. 相似文献108.
This study presents a comparative analysis of protected and exploited Mediterranean Sea food webs using standardized ecological models of two ecosystems: the Central-North Adriatic Sea (highly exploited) and the Miramare Natural Marine Reserve (Northern Adriatic Sea, protected since 1986). Food webs are analysed in terms of structural and functional traits including trophic levels, transfer efficiency, trophic role of species and keystoneness, trophic spectra and other synthetic ecological indicators. Our results highlight similarities between food webs of the Adriatic Sea, differences that are likely due to the impact of fishing and differences that should be related to other factors. Analysis of biomass distribution along functional groups and of trophic spectra indicates that protected area presents higher mean trophic level of the community, higher biomasses, lower production and generally lower transfer efficiency than fished area. Our results indicate that measures of food web complexity (system omnivory index and ascendency), as well as pelagic/demersal and fish/invertebrates biomass ratios are higher in the protected area than in exploited ecosystem due to fishing-induced changes. Thus, this study highlights that marine protected areas can efficiently contribute to maintain structural and functional traits of marine ecosystems. 相似文献
109.
Cacciò SM Antunovic B Moretti A Mangili V Marinculic A Baric RR Slemenda SB Pieniazek NJ 《Veterinary parasitology》2002,106(4):285-292
The morphologically small Babesia species isolated from naturally infected dogs in Europe, Japan, and US are described as Babesia gibsoni despite the fact that molecular techniques show that they should be assigned to two or three separate taxons. The morphologically large Babesia isolated from dogs in Europe, Africa, and US were generally classified as B. canis until it was proposed to distinguish three related, albeit genetically distinct subspecies of this genus, namely B. canis canis, B. canis rossi, and B. canis vogeli. The insight into the molecular taxonomy of canine piroplasms is, however, limited because only partial small subunit ribosomal RNA (ssrRNA) sequence data exist for two species from the B. canis group. In this work, we molecularly characterised natural Babesia infections in 11 dogs from Croatia, France, Italy, and Poland. These infections were diagnosed as caused by B. canis canis and B. canis vogeli based on the analysis of the complete sequence of the ssrRNA genes. Phylogenetic analysis confirmed that the large Babesia species of dogs belong the to the Babesia sensu stricto clade, which includes species characterised by transovarial transmission in the tick vectors and by exclusive development inside the mammalian host erythrocytes. The new data facilitate the reliable molecular diagnosis of the subspecies of B. canis. 相似文献
110.
Forest structure and live aboveground biomass variation along an elevational gradient of tropical Atlantic moist forest (Brazil) 总被引:1,自引:0,他引:1
Luciana F. Alves Simone A. Vieira Marcos A. Scaranello Plinio B. Camargo Flavio A.M. Santos Carlos A. Joly Luiz A. Martinelli 《Forest Ecology and Management》2010
Live aboveground biomass (AGB) is an important source of uncertainty in the carbon balance from the tropical regions in part due scarcity of reliable estimates of live AGB and its variation across landscapes and forest types. Studies of forest structure and biomass stocks of Neotropical forests are biased toward Amazonian and Central American sites. In particular, standardized estimates of aboveground biomass stocks for the Brazilian Atlantic forest are rarely available. Notwithstanding the role of environmental variables that control the distribution and abundance of biomass in tropical lowland forests has been the subject of considerable research, the effect of short, steep elevational gradients on tropical forest structure and carbon dynamics is not well known. In order to evaluate forest structure and live AGB variation along an elevational gradient (0–1100 m a.s.l.) of coastal Atlantic Forest in SE Brazil, we carried out a standard census of woody stems ≥4.8 cm dbh in 13 1-ha permanent plots established on four different sites in 2006–2007. Live AGB ranged from 166.3 Mg ha−1 (bootstrapped 95% CI: 144.4,187.0) to 283.2 Mg ha−1 (bootstrapped 95% CI: 253.0,325.2) and increased with elevation. We found that local-scale topographic variation associated with elevation influences the distribution of trees >50 cm dbh and total live AGB. Across all elevations, we found more stems (64–75%) with limited crown illumination but the largest proportion of the live AGB (68–85%) was stored in stems with highly illuminated or fully exposed crowns. Topography, disturbance and associated changes in light and nutrient supply probably control biomass distribution along this short but representative elevational gradient. Our findings also showed that intact Atlantic forest sites stored substantial amounts of carbon aboveground. The live tree AGB of the stands was found to be lower than Central Amazonian forests, but within the range of Neotropical forests, in particular when compared to Central American forests. Our comparative data suggests that differences in live tree AGB among Neotropical forests are probably related to the heterogeneous distribution of large and medium-sized diameter trees within forests and how the live biomass is partitioned among those size classes, in accordance with general trends found by previous studies. In addition, the elevational variation in live AGB stocks suggests a large spatial variability over coastal Atlantic forests in Brazil, clearly indicating that it is important to consider regional differences in biomass stocks for evaluating the role of this threatened tropical biome in the global carbon cycle. 相似文献