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731.
Jose Alberto Acosta Angel Faz Silvia Martínez-Martínez Joselito Modancia Arocena 《Journal of Soils and Sediments》2014,14(4):819-828
Purpose
Green areas in urban parks are special ecosystems because of social and ecological benefits.Materials and methods
We investigated the ecological impacts of green spaces by comparing the properties of soils, with emphasis on metals, in green spaces and bare soil playgrounds in four urban parks in Murcia City (SE Spain).Results and discussion
Organic matter (OM) contents in green areas are higher than playground soils due to litter decomposition from grass. Cation exchange capacity in green areas ranged from 10 to 14 compared to 2 cmol+kg?1 in playground soils, showing the effect of OM (and clay) contents in green areas due to the presence of vegetation. Soil total metal contents in urban parks are below the environmental threshold values for soils in Spain and other European countries, and come from atmospheric deposition and fertilizers application. Diethylenetriaminepentaacetic acid (DTPA)-extractable soil Cd in green areas (4% of total Cd) is lower than in playground soils (58% of total Cd). We attributed the reduced DTPA- and water-extractable Cd, Co, Cr, Mn, and Mo to the formation of stable complexes between metals and OM produced from the continued decomposition of roots of grasses in green spaces.Conclusions
Grasses accumulate less metal than soils except in roots where Cr and Zn are higher than soils by as much as 31 and 17%, respectively. We conclude that grasses increased (up to 8×) soil OM content in green areas to significantly increase the ability of soils to accumulate nutrients for plants, and to retain and reduce the mobility and toxicity of metals in soils in urban parks. 相似文献732.
The spindle checkpoint delays sister chromatid separation until all chromosomes have undergone bipolar spindle attachment. Checkpoint failure may result in chromosome mis-segregation and may contribute to tumorigenesis. We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Depletion of Hec1 impaired chromosome congression and caused persistent activation of the spindle checkpoint, indicating that high steady-state levels of Mad1/Mad2 complexes at kinetochores were not essential for checkpoint signaling. Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint-deficient cells. 相似文献
733.
Ehrlichia canis has a worldwide distribution, but clinical manifestations may vary geographically. We selected 129 dogs to determine prevalence of ehrlichiosis in dogs with anemia, thrombocytopenia, or ticks presented to a Veterinary Teaching Hospital in South Brazil. Of the 129 dogs, 68 carried the brown dog tick (Rhipicephalus sanguineus), 61 had thrombocytopenia (platelet count <150,000/microl), and 19 had anemia (PCV < 22%). Twenty dogs fulfilled more than one inclusion criteria. Ehrlichiosis was diagnosed by positive amplification of ehrlichial DNA by PCR using primers ECC and ECB that amplify a sequence of the 16S rRNA gene. Presence of E. canis was confirmed by cleavage of the amplified DNA using endonucleases HaeIII and AvaI. Fourteen of 68 (21%) dogs with ticks had ehrlichiosis, whereas 12 of 61 (20%) dogs presented with thrombocytopenia and 4 of 19 (21%) anemic dogs had ehrlichiosis. Similar results were obtained in dogs with thrombocytopenia and anemia (one of eight positive) and in dogs with thrombocytopenia and ticks (two of seven positive). All four dogs with anemia and ticks, and the dog that fulfilled all inclusion criteria yield no amplification of ehrlichial DNA by PCR. Based on our results, one in each five dogs infested by the brown dog tick, with anemia or thrombocytopenia had ehrlichosis. Contrary to widespread believe, ehrlichiosis was not the main cause for thrombocytopenia in our region. 相似文献
734.
Jesús Hernndez Eneida Soto-Canevett Araceli Pinelli-Saavedra Mnica Resendiz Silvia Y. Moya-Camarena Kirk C. Klasing 《Veterinary immunology and immunopathology》2009,131(1-2):9-16
In order to analyze the effect of vitamin E on Th1 and Th2 cytokine production, porcine peripheral blood mononuclear cells (PBMC) were isolated from healthy pigs (n = 8) and cultured with either 0, 10, 50, or 100 μM of vitamin E (α-tocopherol). PBMC were stimulated with PHA for either, 24 h to determine: (a) the concentration of tocopherol incorporated into the cell membrane, (b) cytokine production and (c) Th1 and Th2 regulators gene expression; or 72 h to determine the proliferation of PBMC. Vitamin E was incorporated into the PBMC in a dose dependent manner, giving as a result a high proliferation of cells irrespective of the dose of vitamin E used. Regarding cytokine production, vitamin E consistently decreases the mRNA expression and the percentage of cells producing IL-10. Vitamin E did not influence the production of IFN-γ but the lowest level of vitamin E (10 μM) was sufficient to maximally increase the proportion of cells producing IL-2, to diminish IL-4, and discreetly increase the mRNA expression of TBX21 vs. GATA3. In conclusion, our results revealed that vitamin E is able to suppress IL-10 production and to influence the production of IL-2, IL-4, and maybe TBX21. Vitamin E clearly has immunomodulatory effects, though further work in vivo to determine the physiological nature of these effects is warranted. 相似文献
735.
Roberta Teta Germana Esposito Karishma Kundu Mariano Stornaiuolo Silvia Scarpato Antonino Pollio Valeria Costantino 《Marine drugs》2022,20(4)
In this study, a strain of Anabaena flos-aquae UTEX 1444 was cultivated in six different concentrations of iron (III). Cultures were extracted with organic solvents and analyzed using our dereplication strategy, based on the combined use of high-resolution tandem mass spectrometry and molecular networking. The analysis showed the presence of the siderophores’ family, named synechobactins, only in the zero iron (III) treatment culture. Seven unknown synechobactin variants were present in the extract, and their structures have been determined by a careful HRMS/MS analysis. This study unveils the capability of Anabaena flos-aquae UTEX 1444 to produce a large array of siderophores and may be a suitable model organism for a sustainable scale-up exploitation of such bioactive molecules, for the bioremediation of contaminated ecosystems, as well as in drug discovery. 相似文献
736.
- Group membership is a key attribute of animal societies and central to the study of social structure in several taxa. However, social structure analyses are sensitive to the way data are collected and associations defined.
- In this study, a time–space method was used to investigate the social structure of common bottlenose dolphins Tursiops truncatus observed and photographed across 7 years in the semi-enclosed Gulf of Corinth, Greece. Instead of adopting traditional group definitions, individuals were considered as being members of the same group if photographed within a specific time and space window. This approach can be applied post hoc across studies and can offer advantages under challenging sampling conditions (e.g. when dealing with groups spread over vast areas or when group membership is otherwise hard to assess).
- Dolphins were mostly found around coastal cage aquaculture facilities farming European sea bass Dicentrarchus labrax and gilthead seabream Sparus aurata.
- Dolphins formed clusters largely or entirely composed of individuals of the same sex, suggestive of sex-based homophily. Habitat partitioning was not detected: there was substantial spatial overlap among dolphin clusters, with all individuals using a relatively small area in the northern portion of the Gulf, where most of the productive fish farms were located. Associations between females were stronger than those between males, and daughters tended to stay in the group of their mothers.
- Sex-based social clustering may allow females and calves to limit interactions with potentially aggressive males, while individuals of both sexes benefit from prey concentrated around fish farms.
- Adaptation to foraging around farms can result in trade-offs between the costs and benefits of nourishment and social interaction. This may have both positive or negative effects on the animals that should be considered in the context of ensuring their favourable conservation status.